lecture 25 web: pollev.com/ucibio text: to: 37607 type in: 169964
TRANSCRIPT
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Lecture 25
Web: pollev.com/ucibio
Text: To: 37607Type in: 169964 <your
question>
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Drug therapy
THF metabolism inhibitors would have strongest effect on:
A. Stem cells
B. Cancer cells
C. All cells
D. All dividing cells
E. No cells
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Purines Uric acid
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Pyrimidines MeMalonyl semialdehyde
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Salvage pathways
GMP
IMP
AMP
HGPRT
APRT
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The future
Disease phenotype treatmentDrug design & developmentProtein engineering
Industrial applications
Diagnostics
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Metabolic engineering
Engineer a whole pathway?Large scale production?
Biosynthesis:- Fuel- Nutraceuticals
What else?!?!!
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Example of metabolic engineering
Patnaik, R. and Liao, J. (1994). "Engineering of Escherichia coli central metabolism for aromatic metabolite production with near theoretical yield". Appl. Environ. Microbiol. 60(11):3903-3908
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Example of metabolic engineering
Patnaik, R. and Liao, J. (1994). "Engineering of Escherichia coli central metabolism for aromatic metabolite production with near theoretical yield". Appl. Environ. Microbiol. 60(11):3903-3908
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Example of metabolic engineering
Patnaik, R. and Liao, J. (1994). "Engineering of Escherichia coli central metabolism for aromatic metabolite production with near theoretical yield". Appl. Environ. Microbiol. 60(11):3903-3908
Contr
ol Pps
Pps
* Pps
#
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-Omics = Metabolomics!
ENTIRE collection of metabolites!“Metabolome”
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Metabolome & drugs
Kaddurah-Daouk, Rima, Bruce S. Kristal, and Richard M. Weinshilboum. "Metabolomics: a global biochemical approach to drug response and disease." Annu. Rev. Pharmacol. Toxicol. 48 (2008): 653-683.
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Discovering diagnostic markers
Kenny, Louise C., et al. "Novel biomarkers for pre-eclampsia detected using metabolomics and machine learning." Metabolomics 1.3 (2005): 227-234.
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Differentiating disease conditions
Kaddurah-Daouk, Rima, Bruce S. Kristal, and Richard M. Weinshilboum. "Metabolomics: a global biochemical approach to drug response and disease." Annu. Rev. Pharmacol. Toxicol. 48 (2008): 653-683.
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The Human Metabolome project
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Synthetic biology – “creating” life…
“Build” a genome from scratch?What is “minimal” genome?
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…and beyond!
Protein + metabolic engineering
Any compound, any time!
“Download” vaccine to personal biofactory?!