Learning how to use the Learning how to use the microscopemicroscope

(COPY NOTES)1.There are 2 kinds of microscopes:

A. Simple: one lensone lens (magnifyingmagnifying glass)

B. Compound (Complex): 2 or more lenses

Light Microscope

Parts of the MicroscopeParts of the Microscope

1. Revolving Revolving NosepieceNosepiece

Parts of the MicroscopeParts of the Microscope

2. 2. Lens/ObjectiLens/Objectiveve

Parts of the MicroscopeParts of the Microscope

3. 3. StageStage

Parts of the MicroscopeParts of the Microscope

4. 4. Disc Disc DiaphragmDiaphragm

(controls amount (controls amount of of lightlight))

Parts of the MicroscopeParts of the Microscope

5. 5. Light Light source:source:

Lamp or lightLamp or light

Parts of the MicroscopeParts of the Microscope

6. 6. BaseBase

Parts of the MicroscopeParts of the Microscope

7. 7. EyepieceEyepiece

Parts of the MicroscopeParts of the Microscope

8. 8. ArmArm

Parts of the MicroscopeParts of the Microscope

9. 9. Stage ClipsStage Clips

Parts of the MicroscopeParts of the Microscope

10. 10. Coarse Coarse Focus/Focus/

AdjustmentAdjustment

Parts of the MicroscopeParts of the Microscope

11. 11. Fine Fine Focus/Focus/

AdjustmentAdjustment

Calculating MagnificationCalculating Magnification

MultiplyMultiply lens powers together! lens powers together!

• EyepieceEyepiece: 10 X10 X by itself

• Low PowerLow Power: : 44X; redred color; and a TOTAL magnification of 40 X40 X mainly used for large objects/scanninglarge objects/scanning

• Medium PowerMedium Power: : 1010 X, X, yellowyellow color; TOTAL magnification of 100 X100 X

• High PowerHigh Power: : 4040 X; X; blueblue color; and a TOTAL magnification of 400 X; 400 X; mainly used for smaller mainly used for smaller objects/detailsobjects/details

Parts of the Microscope

1.

2.

3.

4.5.

6.

7.

8. 9.

10.

11.

Eyepiece

Revolving Nosepiece

Objective LensStage Clips

StageDiaphragm

Lamp/Light

Base

Arm

Coarse Focus

Fine Focus

Microscope Skills

When you are ready to When you are ready to start:start:

3.Make sure microscope is on LOWLOW power!

4.Put stage all the way UP (you focus by slowly moving stage down)

5.Check the light setting (should be open)

Other Things to Consider:Other Things to Consider:

6.Carrying the Microscope:

Always use 2 hands!Always use 2 hands!

Other Things to Other Things to Consider:Consider:

7.Never use the COARSE FOCUS KNOB while on HIGHHIGH power.

8. When putting the 8. When putting the microscope away (at the microscope away (at the

end of class):end of class):A. It should be on LOWLOW power.B. The stage should be all the

way DOWNDOWN (do this with the coarse focus knob!)

C. The light switch should be OFFOFF

9.TROUBLESHOOTING9.TROUBLESHOOTINGWhat if you can’t get your object What if you can’t get your object

into focus?into focus?

A. Check that you have one of the lenses clicked into place

B. Check that your disc diaphragm is allowing light to pass through (not closed)

TROUBLESHOOTINGTROUBLESHOOTING(CONTINUED)(CONTINUED)

• C. Clean off your lenses using LENS PAPERLENS PAPER. (Also, dirty slides are hard to focus on; so clean them, too!)

• D. GO BACK TO LOW POWER AND START OVER!

• Use a MAGNETICMAGNETIC field to bend beams of light; used to see things at much higher level of detail

• Can magnify images up to 1,000,0001,000,000 times!

There are 2 kinds:

• SCANNING ELECTRON MICROSCOPES (SEM): Allows you to see the SURFACESSURFACES of objects

There are 2 kinds:

• TRANSMISSION ELECTRON MICROSCOPES (TEM): Allow you to see THROUGHTHROUGH an object.

Electron Microscope Images:Electron Microscope Images:

http://www.pbrc.hawaii.edu/bemf/microangela/

Microscopes by Brainpop