The beauty of science

Last classClass policies, etc.PCRVNTRsVisualizing DNADNA is not colored; Cant see in solution.Run mixture through an__________ solution is like JellO. Liquid when hot, solid(ish) when cold--Separate pieces based onSeparating DNAAgarose gel is porous____________ in gel allow DNA to pass through________ DNA passes easily, ____ DNA less easilyAgarose gelhttp://www.youtube.com/watch?v=2UQIoYhOowM

Loading dyeWhy do you need a buffer?Why do you add glycerol?Why do you need the dye?Seeing the DNAEtBr + UV light =Gel/buffer contains ETHIDIUM BROMIDE (EtBr)EtBr in DNA + UV light =Regions in gel with DNA will show a BRIGHT bandhttp://wikidoc.org/index.php/Agarose_gel_electrophoresis

Estimating size of DNASee DNA. But size?

200 bp300 bp400 bp500 bp600 bp1000 bp2000 bphttp://wikidoc.org/index.php/Agarose_gel_electrophoresis

Changing gel parametersIncrease voltageIncrease percentage of gelWhy?Watch out!Increase time of runLAB 2Gel will be poured for youLoad and run gelCheck resultsLab 3 onwards = pour your own gelHow do we study life?I think X BECAUSE YDesign an experiment to PROVE XExperiment:Predict outcomes if X is TRUEPredict outcomes if X is FALSEDo experiment, observe resultsResults; Therefore X is TRUE/FALSEUnexpected results/observations = Discovery!Restriction endonucleasesRE is an enzyme!----RE cutting is random!Designing experimentsTube10X Buffer (L)RE (L)Water (L)1N HCl (L)11.01.0-8.00.021.01.01.07.00.031.01.0-7.01.041.01.01.06.01.051.01.0-6.02.061.01.01.05.02.0Designing experimentsForm a hypothesisDesign an experimentPredictions?LAB 2Design experiment to test ONE variable- Time- Buffer- Enzyme concentrationDiscuss with partner, other groups & TAGenerate experimental designInclude controls!