lactic acid microbiota in mariona rossell garcia postpartum cow · shigella neonatal calf faeces...

INTRODUCTION The bacteria genera most frequently isolated are: Lactic acid microbiota in postpartum cow and her calf Mariona Rossell Garcia June 2017 OBJECTIVES Determination of lactic acid bacteria in cow’s colostrum and milk, and the faeces of their calves Comparison of the isolated microbiota 3 dairy farms Osona 115 colostrum and milk samples 31 faeces samples MATERIALS AND METHODS Agar Man Rogosa Sharpe with cysteine (MRS+C) Different genera of lactic acid bacteria Specific for Bifidobacterium spp. Aerobic conditions Anaerobic conditions Microaerophile conditions Agar Man Rogosa Sharpe (MRS) 37ºC 24 - 48 h Incubation conditions Identification methods Gram Stain Catalase and Oxidase Test API 50 CHL RESULTS CONCLUSIONS ✴ The genera isolated are Bifidobacterium spp., Lactobacillus spp. and Lactococcus spp. No many differences between samples and farms ✴ The counts of the genera Bifidobacterium are higher in colostrum while the counts of Lactobacillus and Lactococcus are higher in milk ✴ The presence of the same genera between meconium and faeces supports the hypothesis of a non sterile gastrointestinal tract of the neonate ✴ Exists a marked variability between individuals and cow’s quarters. For a representative sample of the individual, should be sampled each of the quarters Isolation frequency 0% 7,4 % 14,8 % 22,2 % 29,6 % 37 % Lactic acid bacteria genera Bifidobacterium spp. Lactobacillus spp. Lactococcus spp. Colostrum Milk Faeces Graphic 1. Lactic acid bacteria genera isolated in colostrum, milk and faces samples 32,95 % 35,29 % 33,33 % 34,09 % 35,29 % 36,67 % 32,95 % 29,41 % 30,00 % Colostrum and Milk Kocuria Staphylococcus Streptrococcus Lactic acid bacteria Bifidobacterium, Lactobacillus, Lactococcus and Leuconostoc PROBIOTICS ?? Lactobacillus Bifidobacterium Escherichia Shigella Faeces NEONATAL CALF DIARRHEA Bifidobacterium spp – Lactobacillus spp. and Lactococcus spp. Lactobacillus plantarum Lactococcus lactis Colostrum = Milk Faeces Probiotics’ features Meconium Possible maternal origin Bifidobacterium spp. is more frequently isolated in milk samples, but its counts are higher in colostrum Colostrum and milk samples: High variability between cow’s quarters and between individuals Faeces samples: High variability between individuals No many differences between farms Graphic 2. Representative box plot of cows’ quarters count of each individual, sorted by farms Farm 1 Farm 2 Farm 3

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INTRODUCTION The bacteria genera most frequently isolated are:

Lactic acid microbiota in

postpartum cow and her calf

Mariona Rossell Garcia

June 2017

OBJECTIVES Determination of lactic acid bacteria in cow’s colostrum and milk, and the faeces of

their calves Comparison of the isolated microbiota

3 dairy farmsOsona

115 colostrum and milk samples 31 faeces samples

MATERIALS AND METHODSAgarManRogosaSharpewithcysteine(MRS+C)

Differentgeneraoflacticacidbacteria

SpecificforBifidobacteriumspp.

Aerobicconditions

Anaerobicconditions

Microaerophileconditions

AgarManRogosaSharpe(MRS)

37ºC24-48h

Incubationconditions Identificationmethods

GramStainCatalaseandOxidaseTest

API50CHL

RESULTS

CONCLUSIONS ✴ The genera isolated are Bifidobacterium spp., Lactobacillus spp. and Lactococcus spp. No many differences between samples and

farms ✴ The counts of the genera Bifidobacterium are higher in colostrum while the counts of Lactobacillus and Lactococcus are higher in milk ✴ The presence of the same genera between meconium and faeces supports the hypothesis of a non sterile gastrointestinal tract of the

neonate ✴ Exists a marked variability between individuals and cow’s quarters. For a representative sample of the individual, should be sampled

each of the quarters

Isol

atio

n fre

quen

0 %

7,4 %

14,8 %

22,2 %

29,6 %

37 %