lab 1 powerpoint

INSTRUCTOR – Mrs. Barnacle (Bartha)

Office – Schoo-Bemis 208 Office hours: Mon, Wed & Friday 10-11;

Tues & Thurs 11-12

E-mail – [email protected]

If you can not make it to my office hours, the best way to get in touch with me is by email. I will usually respond within a couple of hours, except on weekends and holidays.

Welcome to Bioscience 123 Lab!

Make sure that you check Moodle daily. You will have a pre-lab quiz every week posted on Moodle. All of your worksheets also will be posted on Moodle.

Forum Rules:• Inappropriate language and bullying will not be tolerated

• If you forget to write something, please go back and edit the same post, do not create a new post

• If you are concerned about something written on a forum, please email me immediately

• Please do not ask me a question via the forum. Email me instead.

Pre-Lab Quiz Rule:• The quiz is to be done alone; cheating will not be tolerated

• Please do not leave the quiz open all day. When you are finished taking the quiz, please submit your answers and close the screen.

•If you are having any technically issues with a quiz, please email me right away.

BioscienceGeneral rules

Please turn off your cell phone and put it away. Cells phones are not to be used in lab unless otherwise noted by the instructor.

There are times that you can use the cell phone to take photos during a particular lab. Ask before using a cell phone as a camera.

There is no food or drink allowed in thelab. That includes water bottles andchewing gum.

RULES OF THE LAB

BioscienceGeneral rules

Do not be late – If you come to lab late, you will not be able to make up any

missed work or quizzes.

Do not cheat – Any plagiarism will result

in an automatic zero for the lab for allparties involved.

Wear proper attire (closed toe shoes, long pants, tie long hair back, no tank tops). Wear goggles and gloves when instructed

• 5% of your grade will be participation of the iclicker Questions. You must answer all clicker questions to receive credit and you must get 75% of the questions correct.

• 20% of your grade will be determined by weekly pre-lab quizzes. - They are to be done on Moodle and are to be done alone.

• 20% of your grade will be determined by a in class post-lab quizzes. These will be given

the week after you have the lab and will be about 5 questions.

• 30% of your grade will be determined by worksheets. Theses worksheets are to be handed in the following week after lab. Unless otherwise instructed, the worksheets are used as a guide for the experiments. Fill out the worksheets along with reading the lab manual.

• 15% of your grade will be determined by attendance and participation.

• 10% of your grade will be determined by weekly interpretations. Theses are to be handed in either at the end of lab or the following week (as instructed by me).

BioscienceGrading

iclicker Questions: In class post-lab Quiz:Online pre-lab Quiz:

5%20%20%

Worksheets: Attendance/Participation:Weekly Interpretations:

30%15%10%

I highly suggest that you take notes during the lab lecture.

BioscienceSafety

Basic lab safety rules1. Locate all safety equipment –emergency shower, eye wash stations, emergency electric and gas shutoff valves, first aid supplies

2. Identify all emergency evacuation routes.

3. Closed shoes are required. No open sandals, flip-flops, or any other type of shoe fails to cover the toes. Try not to wear loose-fitting shirts or pants.

4. Tie back long hair

5. No bags on the tops of the lab bench

6. Do not eat, drink, apply make-up, or remove contacts - No gum, candy, etc.

BioscienceSafety BE CAREFUL AND USE COMMON SENSE!

Basic lab safety rules7. No smoking in the building

8. Always be conscious of your hands – do not bite nails, touch eyes, etc.

9. Goggles and gloves are to be worn when instructed by me. - We will be working with chemicals that have potential to harm your eyes/skin and ruin your clothes (don’t wear something to lab that you don’t mind getting stained).

10. Waste disposal – Do not put harmful chemicals (e.g. organic solids/liquids) in the sinks. ASK IF YOU ARE UNSURE ABOUT WASTE DISPOSAL

11. Be careful when handling glass and other sharps - Never use cracked or broken glassware, never force glassware into a waste container, etc.

12. Wash your hands before leaving the lab – you may have been careful, but your neighbor may not be.

BioscienceSafety BE CAREFUL AND USE COMMON SENSE!

Lab 1: Data Collection Key Lab equipment

erlenmeyer flask Beaker Pipette

scale Test tube Graduated cylinder

Lab 1: Data Collection

Procedure 1.4: The effect of food type on yeast growth

Scientific Method

•Make insightful observations•Pose and clarify testable questions•Formulate hypothesis•Do experiments to gather data•Quantify the data•Test the hypothesis•Refine hypothesis and re-test•Answer the question and make conclusions

Which is the null hypothesis?

A.Rainfall volume is higher when the temperature is above 70 degrees outside.

B.There is no significant difference between rainfall volume and outside temperature.



Which hypothesis will you use for your experiment? Which is testable?

A.Null Hypothesis

B.Alternative Hypothesis

C.Neither

D.Both

Now write your null and alternative hypothesis on your worksheet.



Purpose of the experiment:Add glucose to one test tube of yeast, add protein to another test tube of yeast and

measure the amount of CO2 produced. As yeast grow, they release CO2. The amount of

growth is proportional to and can be measured by the amount of CO2 given off.

The independent variable in this experiment is ________ and the dependent variable is ______.

a. Food molecule; waterb. water; CO2c. CO2; Food moleculed. Food molecule; CO2

The amount of CO2 produced is DEPENDENT on the amount of food molecule (independent variable) added.



Experiments need replications to verify naturally occurring variation of the results

Each group will be conducting the same experiment; therefore there will 6 replications.

Purpose of a control: To validate that the results are due only to the treatment (independent) variable.

Each group will get three test tubes (instead of 12 as noted in the lab book). Test tube 1 will contain 5mLs of 5% glucose; test tube 2 will contain

5mLs of 5% protein and test tube three will contain 5mL of tap water.

Follow procedure 1.4, steps 5 to 9


Accuracy vs. Precision

A B

In the above picture, (A) represents _____ while picture (B) represents_______.

A.Precise, AccurateB.Accurate, PreciseC.Accurate, AccurateD.Precise, Precise

Accuracy- True ValuePrecision- exactness and reproducibility


Procedure 1.5: Quantify and summarize the data

Analyze the data

1. Measure CO2 production in mm.2. Calculate the mean CO2 production among the 6 groups.3. Subtract the mean of CO2 production in the control from the mean of the CO2 production in the glucose test tube. Do the same for the Protein test tube. 4. Record the range of mms of both the Glucose test tube and Protein test tube.5. Calculate the SD for Glucose and Protein

Testing a Hypothesis

Verify that the means are significantly different by calculating that means plus or minus ½ of the SD do not overlap. Fill out your calculations and answer the questions. Is your null hypothesis accepted or rejected?

lab 1 powerpoint

Education