ista – variety committee...committee members chair 1 berta killermann de-germany vice-chair 2 ana...
TRANSCRIPT
ISTA – Variety Committee
Day, 19 June 2010 Agenda Point (16:00-17:00)
... the Technical Committee for Species andVariety Testing
Report of …
Activities
Working program
Special projects
Berta Killermann
Committee members
Chair 1 Berta Killermann DE-Germany
Vice-chair 2 Ana Laura Vicario AR-Argentina
Members 3 Emanuela Casarini IT-Italy
4 Rainer Knoblauch DE-Germany
5 Kae-Kang Hwu TW-Taiwan
6 Cheryl Dollard CA-Canada
7 David Zhang FR-France
8 Chiara Delogu IT-Italy
9 Ulf Kjellström SE-Sweden
10 Eszter Timar HU-Hungary
11 Jerzy Drzewiecki PL-Poland
12 Hedwich Teunissen NL-Netherland
13 Savitha H. Reddy IN-India
14 Kalyn Brix-Davis US-United States
15 Wendy Carnegie CA-Canada
16 Michael Lovelace US-United States
Working Group – DNA-based methods
Leader 1 Emanuela Casarini IT-Italy Group leader
Co-workers 2 Ana Laura Vicario AR-Argentina soybean
3 Kae-Kang Hwu TW-Taiwan rice
4 Cheryl Dollard CA-Canada
5 Cesar Olsina AR-Argentina
6 Daniel Perry CA-Canada wheat
7 Marie-Jose Cote CA-Canada
8 Elisa Vieira BR-Brazil
9 Kalyn Brix-Davis US-United States
10 Kunusoth Keshavulu IN-India
11 Eun-Hee Soh KR-Korea
12 Kim Kenward CA-Canada
13 Benjamin Kaufman US-United States maize
14 Christelle Forzy FR-France
15 Chiara Delogu IT-Italy
16 Fengge Wang CN-China
17 Gaspar Malone AR-Argentina
Activities - WG DNA-based methods
2009
First ISTA Workshop on Molecular Markers for Variety Identity and Purity at the
University in Bologna, Italy (June 2009)
Comparative Tests (microsatellites - SSRs)
3rd CT for maize, wheat, soybean and rice were run
2010 and later
Comparative Tests (microsatellites , SSRs)
4th CT for soybean and wheat are running
First PT for DNA-based methods is planned for maize (under discussion)
handling of reference-material?
performance of the PT? (a classical PT or a performance based approach)
Development of an accreditation standard (including PTs)
a classical accreditation standard
performance based approach, like for GMO testing
Working Group – electrophoresis
Leader 1 Chiara Delogu IT-Italy
Co-workers 2 Rainer Knoblauch DE-Germany
3 Jerzy Drzewiecki PL-Poland
4 Savitha H. Reddy IN-India
5 Maria Alicia Loray AR-Argentina
6 Kalyn Brix-Davis US-United States
7 Kunusoth Keshavulu IN-India
8 Ravi Age IN-India
9 Yujia Wu US-United States
10 Berta Killermann DE-Germany
! We are looking for further co-workers !
Activities – WG electrophoresis
2009
Comparative Tests
Improvement of ISTA A-PAGE method for the verification of Triticum
Test leader Ana Laura Vicario (Argentina)
Design 3 wheat samples (ISTA method)
3 wheat samples (in-house method)
Participating labs Rainer Knoblauch - LTZ - Germany
M. Alicia Loray - INASE - Argentinia
Berta Killermann - LfL - Germany
Chiara Delogu - ENSE - Italy
Trevor Mazutinec - Bio Vision Seed Lab - Canada
Silvia Zanetti - ART - Switzerland
Jerzy Drzewiecki - IHAR - Poland
Status quo data analysis (STACOM)
forward a Rules proposal in 2010
Activities – WG electrophoresis
2010
Comparative Tests
SDS-PAGE for the discrimination of Triticum and xTriticosecale
Test leader Chiara Delogu (ENSE, Italy)
Participating labs we are looking for
Status quo test plan and the technical protocol will be presented
and discussed in the open VARCOM meeting
IEF for the discrimination of Triticum and xTriticosecale
Test leader not known at present
Status quo under discussion
SDS-PAGE, IEF for the discrimination of Festuca and Poa
Status quo under discussion
Activities – WG Electrophoresis
2010
ISTA Pre-Congress Workshop on Species and Variety Testing – electrophoresis
(Federal Plant Variety Office, Hanover, Lower Saxony, Germany)
Activities – WG Electrophoresis
Activities – WG Electrophoresis
Working Group – conventional methods
Leader 1 Michael Lovelace US-United States
Co-workers 1 Benno Voit DE-Germany
3 Wendy Carnegie CA-Canada
4 Berta Killermann DE-Germany
5 Gerry Hall UK-United Kingdom
6 ...
! We are looking for further co-workers !
Activities – WG conventional methods
2010 and later
Engagement in this field of work is of less interest
old fashioned methods but we urgently need this methods
Change in the leadership
where should we focus our activities in future ?
how can we handle this ?
how should we organize this working group ?
What can be done – how can we reach these aims?
development of a questionnaire concerning the need and development for
methods of variety control in laboratories and/or in field plots
catch new challenges (look for methods for testing herbicide tolerances on
seedlings by means of germination tests - greenhouse trials)
comparative tests
...
Projects for the future
Establishment of a database for an inventory of methods for species and variety
testing
electronical database (highly sophisticated)
working sheets (ring binder)
Revision of the existing four handbooks with the aim to have only one handbook
in loose-sheet format with 4 – 5 chapters and a harmonisation in the method
description
Electrophoresis Testing
Rapid Chemical Identification Techniques
Growth Chamber – Greenhouse Testing Procedures: Variety Identification
Laboratory Tests for Variety Determination with fungal Pathogens
DNA-based Methods (new capter?)
Organisation of further ISTA Workshops on Species and Variety Testing
list of possible local organizers (homepage)
list of lecturers (homepage)
safe time and money
Requests in 2009 / 2010
Can you give support (documents, statistics) for conducting an in-house
proficiency test round on hybrid purity testing (06/2009 India)
Assistance in testing maize seed intended for export, in the case when it is not
processed (07/2009 Serbia)
Assistance for bying an appropriate equipment for IEF (07/2009 Czech Republic)
Informations about the status quo and the future aims about the use of DNA
marker technology (SSR, etc.) for hybrid purity testing and why only IEF methods
are incorporated in the Rules – don`t you feel that with the recent advancements
and automation DNA technologies could be more rapid, reliable and economic?
(08/2009 India)
Assistance for bying the correct chemicals for A-PAGE (11/2009 Czech Republic)
How can we analyse the purity of rice varieties? (11/2009 Uganda)
Are methods existing to differentiate varieties of soybean seed lots related to
seedling or hypocotyl length? (12/2009 Argentina)
How can I test the measurement uncertainty in electrophoresis methods?
(02/2010 Serbia)
How can I become a member of the variety committee, especially in the working
group DNA-based methods?
Acknowledgement
Ana Laura VicarioKalyn Brix Davis
Chiara Delogu
Hedwich Teunissen
Cheryl Dollard
Kae-Kang Hwu
Emanuela Casarini
Eszter Timar
Rainer Knoblauch
Jerzy Drzewiecki
Savitha H. Reddy
Wendy Carnegie Ulf Kjellström
David Zhang
Berta Killermann
Michael Lovelace
Open Meeting of the Variety Committee
If you have any
questions
ideas
comments or …
please contact any of us or join the open meeting of the Variety Committee
Date: June 19th
Time: 19:00 – 20:30
Room: Meeting Room 2, 1st Floor
(Isabellensaal, rechts)
if you want to become a
member
co-worker of the Variety
Committee