immunology and serology - · pdf fileimmunology and serology dvt2153 ... • louis...
TRANSCRIPT
![Page 1: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/1.jpg)
IMMUNOLOGY AND SEROLOGY
DVT2153Lecture I
Universiti Malaysia kelantanFaculty oF veterinary medicine
Dr. Erkihun Aklilu2/13/2012 1
![Page 2: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/2.jpg)
IMMUNOLOGY
Immunology:
The study of organisms’ body protection from foreigninvading/disease causing microorganisms.
The study of mechanisms by which an animals/humanbody is protected from infectious agents or foreignsubstances.
A science that deals with the immune system and the cell-mediated and humoral aspects of immunity and immuneresponses (Merriam Webster Dictionary)
2/13/2012 2
![Page 3: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/3.jpg)
ImmunIty
What is immunity?
Immunis (Latin) for "exempt“
Exempt: Free from an obligation or liability imposed on others.
Immunity: the state of being exempted(excluded/freed/absolved/excused /relieved). E.g. from disease/infection.
2/13/2012 3
![Page 4: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/4.jpg)
Brief History of Immunology 12th century Chinese observation
Deliberately infected infants with scabs fromsmallpox lesions: Variolation– Less mortality– Protection from further infection
Concepts of variolation spread to Europe
2/13/2012 4
![Page 5: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/5.jpg)
Brief History of Immunology
Rinderpest (Cattle Plague): Heavy loss ofcattle population through out Europe forhundreds of years
Vague similarity of lesions between Smallpoxand Rinderpest diseases Possibility of variolation suggested (1794) Soaking of small strings in the nasal discharge of
an infected cow Insert into an incision in the dewlap of healthy
animals- The practice became very popular2/13/2012 5
![Page 6: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/6.jpg)
Brief History of Immunology In 1798, Edward Jenner (an English Physician) demonstrated
that a scab from a cowpox lesion can replace smallpox scabs for variolation.
Dramatically reduced the risks of variolation with smallpox dramatically
This procedure was called: Vaccination (from ‘Vacca’ for cow in Latin).
Eventually led to the eradication of smallpox from the world in 1970s
2/13/2012 6
![Page 7: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/7.jpg)
Brief History of Immunology Once variolation was accepted similar
approaches were used
Ovination: To protect sheep from sheep pox
Bovine pleuropneumonia: A piece of tissuefrom infected lung was inserted into anincision in the tail- tail falls off and theanimal gets immuned
2/13/2012 7
![Page 8: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/8.jpg)
Brief History of Immunology
• Louis Pasteur (1879) investigated Fowl cholera(caused by Pasteurella multocida)
• Birds inoculated with old P. multocida cultures wereresistant when challenged by the same bacteria fromfresh culture. Louis Pasteur’s Investigation.ppt
• A breakthrough!
• Pasteur also developed Anthrax vaccine, Rabiesvaccine
2/13/2012 8
![Page 9: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/9.jpg)
Brief History of Immunology Daniel Salmon and Theobald Smith from US
demonstrated that dead organisms can also beused for immunization.
Heat-killed isolates of Salmonella choleraesuis(Bacillus suipestifer) were used to protect pigeonsfrom hog cholera
Von Behring and Kitasato from Germany showedthat filtrates from cultures of Clostridium tetanicould protect animals from tetanus.
2/13/2012 9
![Page 10: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/10.jpg)
The Immune System• The immune system is composed of many
interdependent cell types that collectively protectthe body from infectious agents (bacteria,parasite, fungi, virus) and from the growth oftumor cells.
2/13/2012 10
![Page 11: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/11.jpg)
The Immune System
The immune system must be able to:differentiate between material that is a normalcomponent of the body (“self”) and materialthat is not native to the body “nonself”
In vertebrates a highly specialized receptors fordiscriminating between ”self” and “nonself”body components
2/13/2012 11
![Page 12: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/12.jpg)
The Immune System
Specialized receptors for discriminating between ”self” and “nonself” substances
2/13/2012 12
![Page 13: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/13.jpg)
The Immune System Produces coordinated response to the introduction of
foreign substances or antigens into the body Organizationally divided into two complementary arms:
– The Innate (Native or Natural) Immune System– The Adaptive (Acquired or Specific) Immune System
2/13/2012 13
![Page 14: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/14.jpg)
Innate Immune System Provides the body’s early line of defense against
microbial invaders
Comprises four types of definitive barriers
– Anatomic or Physical (skin, mucous membranes)
– Physiologic (temperature, pH, and chemicals such aslysozyme, complement, and some interferons)
– Phagocytic (monocytes, neutrophils, macrophages)
– Inflammatory events
2/13/2012 14
![Page 15: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/15.jpg)
Innate Immune SystemCommon characteristics of innate immune
defenses
– Present intrinsically (with or without previousstimulation)
– Have limited specificity for shared structures ofmicrobes
– Are not enhanced in activity via repeated exposure
– Have limited diversity of expression
2/13/2012 15
![Page 16: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/16.jpg)
Adaptive/Acquired Immune System
• Once the barriers of the immune response havebeen breached, the adaptive immune system isactivated
• Acquired immune system is activated inantigen-specific fashion to eliminate antigensand provide lasting protection
2/13/2012 16
![Page 17: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/17.jpg)
Adaptive/Acquired Immune System
Components of the Adaptive Immune System
Lymphocytes • T cells and B cells• Plasma cells (end cells of B-lymphocyte differentiation)
Antigen-presenting cells (Macrophages, B cells, and dendritic cells)
2/13/2012 17
![Page 18: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/18.jpg)
Adaptive/Acquired Immune System
Common Characteristics
Specific for particular antigen and are specialized toprovide the best protection
Diverse in their specificity
Enhanced with each repeated exposure (i.e. expressimmunologic memory)
Capable of self/non-self recognition
Self-limiting
2/13/2012 18
![Page 19: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/19.jpg)
Comparisons Between Innate Immune System and Adaptive Immune System
2/13/2012 19
![Page 20: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/20.jpg)
Interaction Between Innate Immune System and Adaptive Immune System Innate and adaptive immune systems do not
operate independently of each other:
– Phagocytic cells process and display antigen tofacilitate stimulation of specific T-lymphocytes
– Macrophages secrete immunoregulatory molecules(cytokines), which help trigger the initiation of specificimmune responses
– T-lymphocyte produce cytokines, which enhance themicrobial activities of phagocytes
2/13/2012 20
![Page 21: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/21.jpg)
Interaction Between Innate and Adaptive Immune Systems
Antibodies produced by plasma cells bind topathogens and activate the complement systemto result in the destruction of the invaders
Antibodies produced by B-lymphocytes bindto pathogens and assist with phagocytosis(opsonization)
2/13/2012 21
![Page 22: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/22.jpg)
Interaction Between Innate and Adaptive Immune Systems
2/13/2012 22
![Page 23: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/23.jpg)
SerologyWhat is Serology?
The branch of laboratory medicine that studiesblood serum for evidence of infection and otherparameters by evaluating antigen-antibodyreactions in vitro
Serology is the scientific study of blood serum. Inpractice, the term usually refers to the diagnosticidentification of antibodies in the serum (possibleto detect antigens as well)
2/13/2012 23
![Page 24: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/24.jpg)
Serology Serology as a science began in 1901.
Austrian American immunologist KarlLandsteiner (1868-1943) identifiedgroups of red blood cells as A, B, and O.
From that discovery came therecognition that cells of all types,including blood cells, cells of the body,and microorganisms carry proteins andother molecules on their surface that arerecognized by cells of the immunesystem.
2/13/2012 24
![Page 25: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/25.jpg)
History of Serology
Karl Landsteinar (1868-1943) An Austrian physician,
Landsteiner played an integralpart in the identification ofblood groups.
He demonstrated thecatastrophic effect of transfusingwith the wrong type of blood,
2/13/2012 25
![Page 26: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/26.jpg)
2/13/2012 26
![Page 27: IMMUNOLOGY AND SEROLOGY - · PDF fileIMMUNOLOGY AND SEROLOGY DVT2153 ... • Louis Pasteur (1879) investigated Fowl cholera ... Louis Pasteur’s Investigation.ppt](https://reader033.vdocuments.us/reader033/viewer/2022051406/5ab6e4a97f8b9a156d8e48e0/html5/thumbnails/27.jpg)
Applications of Serology HIV testing Serum HCG (pregnancy) Tests for hepatitis antigens and
antibodies Antibodies to bacteria Hepatitis serology Various animal diseases (e.g
Brucellosis) For epidemiological studies Differentiate vaccinated vs
unvaccinated/infected animals2/13/2012 27