chapter ten - serology
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Chapter Ten - Serology. FRSC 8104 Criminalistics II Professor Bensley. FRSC 8104 – Serology Objectives. Describe the basic components of blood Convey the techniques for collecting and preserving blood evidence at a crime scene. - PowerPoint PPT PresentationTRANSCRIPT
Chapter Ten - SerologyFRSC 8104
Criminalistics IIProfessor Bensley
FRSC 8104 – Serology Objectives1. Describe the basic components of blood 2. Convey the techniques for collecting and
preserving blood evidence at a crime scene.
3. List and describe various forensic tests used to characterize a stain as blood.
4. Understand the concept of antigen-antibody interaction and how it is applied to the origin determination of blood.
FRSC 8104 – Serology Objectives
5. List the A-B-O antigens and antibodies found in the blood for each of the four blood types: A, B, AB, and O.
6. Understand and describe how whole blood is typed.7. Describe the structure of components of semen and
understand the laboratory tests used to characterize seminal stains.
8. Understand the concept of “secretor” status and explain the relevance of such in forensic casework.
9. Calculate basic population genetic problems from forensic casework scenarios.
FRSC 8104 – Serology OutlineI. Introduction
A. What is serology?B. Three main goals of Forensic SerologyC. What is proteomics?
II. BloodA. Formed Elements
1. Erythrocytes (RBC)2. Leukocytes (WBC)
a. Lymphocytesb. Monocytesc. Granulocytes
3. Platelets
B. Fluid (Native Plasma)1. Serum = Plasma - fibrinogen
FRSC 8104 – Serology Outline
http://home.comcast.net/~itpaware/platelets.html
http://www.pennmedicine.org/health_info/bloodless/000209.html
FRSC 8104 – Serology OutlineC. Collection of Blood EvidenceD. Factors Affecting Blood EvidenceE. Presumptive Tests for Blood
1. Introduction – Catalytic Tests2. General Reaction3. False positives and False negatives4. Color Tests
a. Kastle-Meyerb. Tetramethylbenzidinec. Leucomalachite Green
FRSC 8104 – Serology Outline5. Other Presumptive Tests
a. Chemiluminescent Tests (Luminol)b. Fluorescent Tests (Fluorescein)
http://www.webpagedreams.com/ABPD/Investigation.htm
FRSC 8104 – Serology OutlineF. Confirmatory Tests for Blood
1. Crystal Testsa. Takayama Test
FRSC 8104 – Serology OutlineIII. Antigens and Antibodies, Etc.
A. Antigens1. Definition2. Types3. Requirements
B. Antibodies1. Definition2. Types3. Structure4. Classes
C. Antigen/Antibody ReactionsD. Antisera
FRSC 8104 – Serology OutlineAntiserum - nounplural noun: antisera
a blood serum containing antibodies against specific antigens, injected to treat or protect against specific diseases.
FRSC 8104 – Serology OutlineIV. Origin Determination Examinations
A. False PositivesB. False NegativesC. Ring Precipitin Test
http://diverge.hunter.cuny.edu/~weigang/Lecture-syllabus.html
FRSC 8104 – Serology OutlineD. Ouchterlony Double Diffusion
http://www.nfstc.org/pdi/Subject02/pdi_s02_m02_02_b.htm
FRSC 8104 – Serology OutlineV. Blood Group Systems
A. ABO System1. Introduction2. Genetics
http://bloodcenter.stanford.edu/about_blood/blood_types.html
FRSC 8104 – Serology OutlineVI. Body Fluids
A. Seminal Fluid1. Pipeline Effect
a. Pre-Sperm – Cowper’s Glandb. Sperm-Rich – Prostate Glandc. Post-Sperm – Seminal
Vasicular Secretion
2. Spermatozoaa. Morphologyb. Productionc. Their Fate
CHRISTMAS TREE STAIN
FRSC 8104 – Serology Outline
http://www.stlouisco.com/LawandPublicSafety/PoliceDepartment/CrimeLaboratory/BiologyDNAUnit
Nuclear Fast Red and Picroindigocarmine (green)
3. Seminal Plasma4. Prostatic Acid Phosphatase
a. Introductionb. Testing Procedures
5. Choline6. Spermine
a. Barberios Test7. P30 (PSA)
FRSC 8104 – Serology Outline
B. Saliva1. Introduction2. Secretors vs. Non-Secretors3. Formation
a. Parotid Glandb. Submandibular (Submaxillary)
Glandc. Sublingual Glands
4. Composition5. Functions6. Testing
a. Amylase – Phedebas Test
FRSC 8104 – Serology Outline
C. Secretor SystemD. Lewis System
1. Introduction2. Genetics
E. Urine1. Composition2. Properties3. Testing Procedures
FRSC 8104 – Serology Outline