ige test serology
TRANSCRIPT
Immunoglobulin E Disease
Group 23H-Medical Technology
#8 Carla, Glenn Christian#9 Cervantes, Andrea Lourdes
#10 Cheung, Terence#11 Cruz, Carl Dustin
#12 Dantes, Ralph Laurence#13 De Asis, Ana Mikaela
Clinical Manifestation
caused by the release of preformed and newly
synthesized mediators from mast cells and basophils.
Symptoms depends on route of administration.
Anaphylaxis
Most severe type of allergic reaction
Acute reaction with simultaneous involvement of multiple organs
Triggered by glycoproteins or large polypeptide
Agents: Induce Anaphylaxis
• Bees• Wasp • Hornets
Venom
• Peanuts • Dairy products• Shellfish
Foods
• PenicillinDrugs
Signs
Laryngeal edema
Bronchospasm
Skin manifestation (hives)
Diarrhea
Intractable shock
Rhinitis
Most common form of allergy
Symptoms include • runny nose • nasal congestion• itching of the eyes and nose
Airborne• Pollen• mold spores• animal dander• dust
Complication
Sinusitis
Eustachian tube dysfunction
Otitis media
Food allergies
limited to gastrointestinal tract
Diarrhea
Vomiting
Cramping• Cause hives on the skin • Rhinitis • Eczema
Procedure
Open the foil pouch by tearing at the notch and remove the test device and dropper.
Holding the dropper vertically, add four drops of serum or directly to the sample well "S" of the test device.
Read the result at five minutes.
Procedure
Wait for 5 minutes.
Principle of IgE testing
-utilizes the "sandwich"
immunodetection
principle dye-
conjugated polyclonal antibody against
human IgE
-Labeled antibody-dye
conjugate binds to the human IgE molecules present in
the specimen forming an
antigen-antibody-dye
complex
-purple band in control zone "C"
indicates the test is
functioning properly
-purple band in the test zone "T"
indicates IgE present
along with positive
control band
Results and Interpretation
Negative Result
Results and Interpretation
Positive Result
Results and Interpretation
Invalid Result
Thank You!