COST Action MP1301

FTM/Faculty of Technology and Metallurgy

FTMFaculty of Technology and MetallurgyUniversity of BelgradeKarnegijeva 411000 Belgrade - SERBIA

� Complete denomination: Faculty of Technology and Metallurgy, University of Belgrade

� Location (city, country): Belgrade, Serbia

� Dean: Prof. Dr Djordje Janackovic

� Contact person in NEWGEN: Prof. Dr Bojana Obradovic

� Working Group involvment: WG4

� Staff: Dr Jasmina Stojkovska, Jovana Zvicer, Mina Jovanovic,

Dr Djordje Veljovic, Andrea Osmokrovic

� Research topics: biomimetic bioreactors, skeletal tissue engineering,

nanocomposite hydrogels

� Researchers expertises: bioreactor design and operation,

biomaterial evaluation under biomimetic conditions

GENERAL PRESENTATION

COST Action MP1301

BIOMIMETIC BIOREACTORS

FTM/Faculty of Technology and Metallurgy

Biomimicking articular cartilage:perfusion: 10 – 100 µm/sdynamic compression: 337.5 µm/s, 0.1 - 1 Hz, 5-10 % strain

Bioreactor with dynamic compression Perfusion bioreactors

medium

Bioreactor with hydrostatic pressures

up to 30 bar; continuous perfusion

actuator

specimenoil

membranemicrometerscrew

loadsensor

diaphragm

sample

medium in

medium out

COST Action MP1301

Novel Ag/alginate nanocomposites

FTM/Faculty of Technology and Metallurgy

Production of Ag/alginate hydrogels starting from the Ag/alginate solution:

Production of novel Ag/alginate nanocomposites with incorporated silver nanoparticles (AgNPs)

Electrochemical synthesis of AgNPs in alginate solution:

alginatesolution

Ca(NO3)2

+

syringepump

needleAg/alginatemicrobeads

peristalticpump

needleCa(NO3)2

Ag/alginatesolution Ag/alginate

microfibers

Novel Ag/alginate nanocomposites

500 µm 500 µm

Ag/alginate microfibersinitial dried

500 µm

Ag/alginate microbeadsinitial dried

500 µm

Ag/alginate solutions with controlled AgNPs:concentration

low high

y = 3.1406x

R2 = 0.9989

0

0.2

0.4

0.6

0.8

1

1.2

1.4

0 0.1 0.2 0.3 0.4

c [mM]

abso

rban

ce a

t 40

5 n

m

TEM image

size

microbeads microfibers

Bioreactor studies:- biomechanical characterization- Ag release- cell-biomaterial interactions- performance under biomimetic conditions

Hydrothermal synthesis of AgNPs in alginate solutions

AgNP synthesis by chemical reduction in alginate/honey solutions

COST Action MP1301

CYTOTOXICITY STUDIES IN BIOREACTOR CULTURES

FTM/Faculty of Technology and Metallurgy

dynamic compression:1h on/ 1 h off, 0.56 Hz, 10 % straincontinuous perfusion:0.18 ml/min, 15 µm/s

cell/alginate microbead

isolate cells or

Ag/alginate microbeads

explants

strong cytotoxicity in 2D culturesno cyctotoxicity in 3D cultures consistent with in vivo results

3D tissue culture systems provide more reliable functional evaluation of nanomaterials

COST Action MP1301

CHARACTERIZATION OF GELLAN-GUM HYDROGELS REINFORCED WITH BIOACTIVE-GLASS

FTM/Faculty of Technology and Metallurgy

In collaboration with dr Sasa Novak, Jozef Stefan Institute, Slovenia

Hydroxyapatite formation in 2 % w/w gellan-gum discs with 2 % w/w of bioactive-glass (composition: 70 % SiO2, 30 % CaO) over 14 days of continuous perfusion of simulated body fluid

Zvicer J., Gantar A., Veljovic Dj., Novak S., Obradovic B., Evaluation of nano-particulate bioactive-glass reinforced gellan-gum hydrogel regarding the formation of hydroxyapatite under shear stress, Seventeenth Annual Conference YUCOMAT 2015, 31.08- 4.09, 2015, Herceg Novi, Montenegro, Programme & The Book of Abstracts, p. 87

initial

14 d

� Flow rate 1.13 ml/min� SBF exchange every 48 h� 37 oC

RAMAN apectroscopy: characteristic pick for HAp1 at ̴ 960 nm was obtained in all checked spots

FEG-SEM

sample cross-section

COST Action MP1301

FACILITIES

FTM/Faculty of Technology and Metallurgy

Laboratory for animal cell cultivation: biohazard laminar hood, CO2 incubator, microscopes, biomimetic bioreactors

Biomaterial characterization: UV-Vis spectroscopy, XRD, SEM, FTIR antimicrobial activity