expanding your risk management toolbox - on- farm tools
TRANSCRIPT
Expandingyourriskmanagementtoolbox-on-farmtoolsforwaterqualityandpathogenmonitoring
FCOResearchConferenceFebruary1,2017
(GF20251FCO-HMGA)
Objective:
● DevelopPRACTICALmethodsthatgrowerscanuseto:● Trackmicrobialwaterquality–manageRISK● Monitorwatertreatmentsystemperformance● Proactivelymanagewaterqualitythroughoutthewholeproductionsystem
ConcernsforVegetable&HerbGrowers● Foodsafetyinproductionandprocessing● Regulations● Plantpathogensgoingbackontocropland
ConcernsforGreenhouseFlowerGrowers● Plantpathogensgoingintoproductionareasfromfreshorrecycledwater
● Treatmentsystemperformance
Generalwaterquality: Totalaerobicbacteria
3MPetrifilms
● 3MPetrifilmYeast&Mold
● Standardplatingmethods
● Sani-Checkdipslides
● AgDiateststrips
●DNAMultiscan(UofGLabServices)
Toolsforfungalplantpathogens
Sitecode F2Crop CutmixedSystem RecirculatingTreatment UV
DNAMultiscanscores
F2-3 F2-3 F2-3 F2-3 F2-3 F2-4 F2-4 F2-4 F2-4 F2-4Cistern1 Cistern1 Cistern1 Cistern1 Cistern1 Cistern2 Cistern2 Cistern2 Cistern2 Cistern214-May-12 5-Jun-12 5-Jul-12 1-Aug-12 15-Aug-12 14-May-12 5-Jun-12 5-Jul-12 1-Aug-12 15-Aug-12
Botrytiscinerea 1 0 0 0 0 0.00 1 1 0 0 0 0.00 -Fusariumoxysporum 1 0 0 1 1 1.00 0 1 1 1 0 0.50 50F.solani 1 0 0 1 1 1.00 1 1 1 1 1 1.00 0Phytophthorasp. 1 0 0 1 0 0.50 2 0 0 0 0 0.00 100P.cactorum 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -P.capsici 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -P.cinnamomi 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -P.cryplogea 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -P.drechsleri 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -P.fragariae 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -P.infestans 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -P.nicotianae 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -Pythiumsp. 3 1 1 1 1 1.00 4 3 1 1 0 0.50 50P.aphanidermatum 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -P.dissotocum 6 1 2 3 3 3.00 6 8 1 1 0 0.50 83P.irregulere 1 0 0 0 0 0.00 0 0 0 0 0 0.00 -P.polymastum 0 0 0 0 0 0.00 0 1 0 0 0 0.00 -P.sylvaticum 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -P.ultimum 1 0 0 0 0 0.00 0 0 0 0 0 0.00 -Rhizoctoniasolani 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -Sclerotiniasp. 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -Thielaviopsisbasicola 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -Verticilliumalbo-atrum 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -Verticilliumdahliae 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -V.dahliae(verlongisporum) 0 0 0 0 0 0.00 0 0 0 0 0 0.00 -
TargetOrganism
Average(August)
TreatedLeachorRunoffwater-untreatedSampleCode
Average(August)
SampleNameSamplingDate
Averagescore
reduction(Aug)%
Untreatedrecirc.water Treatedwater
OOPS!!
2weeksforresults
DNAMultiscantesting
Plantpathogenremovalbywoodchipbioreactor–DNAMultiscantesting
TargetOrganism
UntreatedSumpWater WoodchipBioreactorTreated
22May12
5June12
5July12
1Aug12
15Aug12
12June13
5Sept13
22May12
5June12
5July12
1Aug12
15Aug12
12June13
5Sept13
Botrytis 2 3 1 0 1 0 0 0 0 0 0 0 0 0Fusarium 0 1 1 1 2 1 1 0 0 0 0 0 0 1Phytophthora 0 1 0 0 0 1 0 0 0 0 0 0 1 0Pythium 0 5 4 5 3 10 1 0 0 0 0 0 1 0Rhizoctonia 0 0 0 0 0 0 0 0 0 0 0 0 0 0Olpidium 0 0 0 0 0 0 0 0 0 0 0 0 0 0Sclerotinia 0 0 0 0 0 0 0 0 0 0 0 0 0 0Thielaviopsis 0 0 0 0 0 0 0 0 0 0 0 0 0 0Verticillium 0 0 0 0 0 0 0 0 0 0 0 0 0 0
$175-$225each
$2-$3each+time
3MPetrifilmforyeast&mold
2-3daystoresults
ProjectCooperatorsCooperator Crops Irrigationsystems Recirc? treatment
Greenhouses(7)
❑Cut❑Potted❑Bedding❑Vegetable
❑Flood•floor,•trough,•Dutchtray❑Drip❑Overhead
YES
❑UV(5)❑Clothfilter(7)❑Peroxide(2)❑Copper❑Chlorinedioxide❑ECA❑Ozone❑WoodchipBioreactor❑Constructedwetland
Vegetablegrowers/Washers
(8)
❑Carrots,beets,parsnips❑Greens❑Asianveg
❑Overheadirrigation❑Widerangeofwashingsystems
Some Nothingtoeverything!
Results-
ToolboxforMicrobialwaterqualityassessment
● 3MPetrifilms-ameasureofRISK● Totalyeast&mold–riskoffungalpathogens● Totalaerobicplatecount(bacterial)–generalwaterquality;riskofbiofilmdevelopmentinpipesanddrippers
● E.coliandtotalcoliforms(onlyiffoodsafetyisaconcern)
● DNAmultiscan–identifiesWHATisthere
Results-
Baselinemicrobialwaterqualitydata(2years)
● treatmentsystemperformanceandgeneralwatermanagement● 7greenhousesystems● 8vegetableproductionandprocessingsystems
● >3000Petrifilmanalyses!!
Typicalresults:treatmentsystemperformance
ECAtreatment
TotalY
east&M
old(cfu
/ml)
1
10
100
1000
27-Jul-19
11-Oct-19
26-Dec-19
11-Ma
r-20
26-May-20
TYM-pretreatmentTYM-posttreatment
Typicalresults:changesoverseasonandmanagement
Freshwatercisterns
cfu/
ml
1
10
100
1000
10000
100000
1000000
10000000
13-Apr-19
28-Aug-19
12-Jan-20
28-May-20
12-Oct-20
TYMbackcisternAerobicbackcisternTY&MfrontcisternAerobicfrontcistern
On-sitecomparisons
Freshwatercistern
cfu/
ml
1
10
100
1000
10000
100000
1000000
10000000
12-Apr-19
4-Sep-19
27-Jan-20
20-Jun-20
12-Nov-20
TYMbackcisternAerobicbackcisternTY&MfrontcisternAerobicfrontcisternAerobiconsiteTY&Monsite
ImplicationsforgrowersTo
talY
east&M
old(C
FU/m
l)
0
1
10
100
1000
10000
TotalDNAMultiscanScore
0 8 15 23 30C1-recirc C2-recircpostUV C3-pond-postUV PondCollectionPit
IncreasedRisk
LowRisk
RISKDetermination
• Lowcounts • Consistentresults • TreatmentsystemOK • ScoutinglooksOK • Trackchangesinlevelswith
watersourceschanges(e.g.pondvsroof)
• Highcounts • Inconsistentresults • Unusualspikesindata • SendforDNAmultiscan? • Extrascoutingforissues? • Check/maintaintreatmentequipment? • Cleantanks,includingfeedtanks
Equipmentalternatives
$159atAmazon.ca$373atAmazon.com
OR
ORRoomtemperatureforAerobicandY&M
OR
Otherparameters
● Teststrips/metersforsanitizerresidualsandotherchemicals● peroxide● chlorine–free&total● chlorinedioxide ● pH● ammonia● nitrate● phosphate
NextSteps
● Workshops–February:● Niagaraarea● HollandMarsharea
● OrderingsuppliesandequipmentthroughFCO● SOONplease!!!!!
● Contactus:● AnnHuber,SRG;[email protected]● JeanineWest,FCO;[email protected]
Acknowledgements● FlowersCanada(Ontario)Inc.,● HollandMarshGrowersAssociation&LSGBCUFproject,● SteeringCommitteeMembers,and● OurGrowers!!
ThisprojectwasfundedinpartthroughGrowingForward2(GF2),afederal-provincial-territorial-initiative.The
AgriculturalAdaptationCouncilassistsinthedeliveryofGF2inOntario.