enzymes models of action
TRANSCRIPT
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Enzyme Action
Learning objective: to describe different models for
enzyme action and to investigate factors which control the
rate of an enzyme reaction
18th October
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Lock and Key
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Lock and Key
However certain substances can bind to the enzyme at sites other than the
Active site and modify its activity (inhibitors/co-factors)
Idea that the enzyme is flexible
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Induced Fit
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Enzyme reactions
enzyme + substrate enzyme-substrate complex
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Enzyme reactions
enzyme + substrate enzyme-substrate complex
E +S ES
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Enzyme reactions
enzyme + productenzyme-substrate complex
E +PES
enzyme + substrate enzyme-substrate complex
E +S ES
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nzyme activity
How fast an enzyme is wor!in"Rate of Reaction
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nzyme activity
How fast an enzyme is wor!in"Rate of Reaction
Rate of Reaction # Amount of substrate chan"ed (or amount product formed)
in a "iven period of time$
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% a t e o f %
e a c t i o n
nzyme activity
&ariable you are loo!in" at
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nzyme activity
'our &ariables
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nzyme activity
'our &ariables
Temperature
pH
Enzyme Concentration
Substrate Concentration
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% a t e o f %
e a c t i o n
Temperature
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% a t e o f %
e a c t i o n
Temperature
* , .
o - denatures
- o
Increase in Activity
0o - inactive
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ffect of heat on enzyme activty
If you heat the protein above its optimal temperature
bonds brea!meanin" the protein loses it secondary and tertiary structure
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ffect of heat on enzyme activty
1enaturin" the protein
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ffect of heat on enzyme activty
1enaturin" the protein
A2I& 3I2 HA453 3HA6
37 38932%A2 47 :745% 'I23
ven if temperature lowered ; enzyme can
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% a t e o f %
e a c t i o n
pH
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% a t e o f %
e a c t i o n
pH
, * . = > ?
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% a t e o f %
e a c t i o n
pH
, * . = > ?
4arrow pH optima
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% a t e o f %
e a c t i o n
pH
, * . = > ?
4arrow pH optima
WHY?
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% a t e o f %
e a c t i o n
pH
, * . = > ?
4arrow pH optima
1isrupt Ionic bonds - 3tructure
ffect char"ed residues at activesite
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% a t e o f %
e a c t i o n
Enzyme Concentration
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% a t e o f %
e a c t i o n
Enzyme Concentration
nzyme oncentration
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% a t e o f %
e a c t i o n
Substrate Concentration
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% a t e o f %
e a c t i o n
Substrate Concentration
3ubstrate oncentration
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