dr. fernando naclerio phd. department of life & sports ... · naclerio et al. non published...
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New Insights on the Use of Protein Supplements for Health and Sport
Dr. Fernando Naclerio PhD. Department of Life & Sports Science
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Diet: “food that is customarily consumed” (Matarese, & Pories, 2014)
SUPPLEMENTATION Providing nutrients over and above their
habitual consumption
“Training Induced Suboptimal Nutrition (TISN)”
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ISSNps (2017)
1.4 2 1.6 1.2
ACSMps (2016)
~3* 0.8
Protein Needs for Athletes (g.kg-1.d-1)
?
Hypo-energetic intake
Muscle mass Adaptation to training
1.8
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To counteract training induced suboptimal nutrition
• Favour optimal training adaptations (volume, intensity, density frequency, duration)
For who?
• Optimise tissue remodelling (increase lean mass) to withstand the exercise related mechanical stress
• Support
• Metabolic demands • Whole body (plasma) proteins turnover • Immune function
Adapted from Phillips, BJN 2012
Why athletes need more proteins?
?
or
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Pattern of Daily Protein ingestion
Protein per meal x meal frequency
7 11 15 18 19 - 20 22 23
Adapted from Phillips, 2014; Morton et al. 2015; ISSNps, 2017
≥0.25 g/kg per meal 0.4 g/kg 0.4 g/kg
5 x 0.25 = 1.25 2 x 0.4 = 0.8
= ~2 g/kg
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Are protein supplements needed to optimise nutrition and training outcomes in athletes?
YES NO
Etc….
Vs.
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Animals & plants proteins sources
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High Quality is determined by - AAs composition - Bioavailability
- Digestibility (AAs kinetic)* - Chemical integrity - Free from nutrient interference
What makes a protein a better one?
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Naclerio et al. non published data Department of Life and Sports Science U of Greenwich
AMINO ACID PROFILE FROM DIFFERENT SOURCES
0
5
10
15
20
25
30
0
10
20
30
40
50
60
70
80
90
100
Whey Isolate
(optipep)
Calcium
caseinate
Whole egg
powder
Bovine
colostrum
Hydrolised
100% All Beef
Hydrolised
HydroBEEF®
Hempt prot.
conc.
Pea Isolate Soy isolate Brown Rice
prot. conc.
g/1
00 g
BC
AA
& L
euci
ne
g/1
00 g
EA
A
EAA
Quality
Leucine
BCAA
-Leu
--Met & Cist
- Hist -Hist -Hist -Met & Cist -Met & Cist
-Trip
--Met & Cist
-Lys
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What else makes a protein source a better food?
Whey Isolate (Isolac) vs Beef Hydrolysate (100% All-Beef)
-100
100
300
500
700
900
1,100
1,300
1,500
Calcium Sodium Potasium Magnesium Phosphorius
mg
/10
0g
pro
du
ct
0
5
10
15
20
25
Iron-hemo Zinc Selenium
mg
/10
0g
pro
du
ct
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Effects of protein administration in athletes
Registered trials at ClinicalTrials.gov, U.S.
National Institutes of Health
Investigation 1 Identifier: NCT02425020
Investigation 2 Identifier: NCT02675348
Investigation 3 Identifier: NCT02954367
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Randomised Controlled Trial Using Parallel groups
Supple
ments
TEST 1
PRE TEST 2 POST
F
RAN
8 weeks
8 weeks
Vs. Vs.
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0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
Beef Whey CHO
mm
Vastus M Thickness
(differences from baseline)
P=0.29 d=0.48
P=0.06 d=1.93
P=0.092 d=1.04
Naclerio et al Eur J Appl Physiol (2017) 117:267–277
Recreationally Trained Males & Females (n=27)
*
Beef + Orange Juice
Whey + Orange Juice
Malt+ Orange Juice
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*p=0.025 for Beef from baseline (pre) to baseline post
Long-term changes in the concentration alpha-defensins HNP1-3 (n=27)
Naclerio et al Eur J Appl Physiol (2017) 117:267–277
0
0.5
1
1.5
2
2.5
3
3.5
4
Pre Workout 1 Pre Workout 24
Alp
ha
- d
efen
sin
s H
NP
1-3
(μ
g/
mL
)
Beef
Whey
CHO
* + Orange Juice
+ Orange Juice
+ Orange Juice
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Resistance Trained Males (n=24)
Naclerio et al IJSNEM doi: 10.1123/ijsnem.2017-0003
-1.0
1.0
3.0
5.0
7.0
9.0
Beef Whey CHO
del
ta s
core
s (
%)
Fat-free mass (kg)
P=0.028
d=1.83
P= 1.00 d=0.02
* *
P=0.034
d=1.97
Beef + Orange Juice
Whey + Orange Juice
Malt+ Orange Juice
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Key points
Supplementation with a carbohydrate-protein (beef) post workout beverage may optimise resistance training outcomes and long-term immune related adaptations in resistance trained athletes.
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Naclerio et al JACN doi: 10.1080/07315724.2017.1335248
Master Trained Triathletes (n=24)
*p<0.05
-20
-15
-10
-5
0
5
10
15
20
Beef Whey CHO
del
ta s
core
s (
%)
* *
d= 1.2 d= -1.5 d= -1.69
Muscle Thickness (mm)
Beef + Water Whey water Malt+ water
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Changes in serum ferritin concentration (ng/mL) pre and post a 10-week nutritional intervention in
male masters (40 to 60 Yr) triathletes (n=24)
-100
-80
-60
-40
-20
0
20
40
60
80
100
Beef Whey CHO
del
ta s
core
s (
%)
P=0.0012 d=2.6
P=0.38 d=0.70
P=0.43 d=-062
Naclerio et al JACN doi: 10.1080/07315724.2017.1335248
Beef + Water Whey water Malt+ water
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Ingesting a post-workout hydrolysed beef protein beverage can be effective in preserving thigh muscle mass and in improving iron status in male master-age triathletes.
Key point
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Blend Proteins
Whey
Caseine Calostrum
Soy
Beef
Pea
hempt
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Males runner post workout beverage (n=27)
Abstract ECSS, 2017 Moreno et al and manuscript in progress
LEAN MASS
*p=<0.05 d>0.8
8
8.5
9
9.5
10
10.5
11
11.5
12
PROT CHO PROT CHO
Le -leg-LEAN Right-leg-LEAN
Lean
Mass(kg)
pre
Post-10-wk
0
2
4
6
8
10
12
PROT CHO PROT CHO
Le -leg-LEAN Right-leg-LEAN
pre
Post-10-wk
* tP=0.065 d=0.56
vs. + Orange Juice + Orange Juice
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Abstract ECSS, 2017 Moreno et al and manuscript in progress
Trunk FAT MASS
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
Kg
Total Trunk FAT Pre
Post
0.0
0.1
0.2
0.3
0.4
0.5
PROT CHO
Kg
Viceral FAT Pre
Post
*
*
*p=<0.05 d>0.8
Males runner post workout beverage (n=27)
vs. + +
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Key point
Ingesting a post-workout blend (beef + whey + CHO) promotes lean mass accretion and reduces trunk fat in young endurance cross country runners.
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Effects of Multi-ingredients on Recovery from Resistance Training
Vs.
59g
CHO 37g
PROT Whey Beef
16g 8.6 7.4
FAT 0.8g
GLUT 2.8g
FAM Pre T Rest W1
W2
W3+T1
T2 T3
Washout
W1
W2
W3+T1
T2 T3
Rest Training + post-workout Supp. n= 6 (REC) n=6 (CHO)
Rest Training + post-workout Supp. n= 6 (REC) n=6 (CHO)
Week 1 Week 2 Week 3 Week 4 & 5 Week 6
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Effects a Multi-ingredient on Recovery from Resistance Training
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0.40
0.45
0.50
Pre tranining Post workout 24 h post workout48 h post workout
Hei
gh
t (m
)
RECOV
CHO
100
120
140
160
180
200
220
240
260
280
300
Pre tranining Post workout 24 h post workout 48 h post workout
Pea
k t
orq
ue
(60
deg
ree/
sec)
RECOV
CHO
*
* p<0.05 to baseline and between groups
Vertical Jump
Isokinetic Strength
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Effects a Multi-ingredient on Recovery from Resistance Training
0
1
2
3
4
5
6
7
24 hr post workout 48 post workout
VA
S s
core
RECOV
CHO
* p< 0.05; **p<0.01
**
**
0.00
2.00
4.00
6.00
8.00
10.00
12.00
Post workout 1 Post Workout 2 Post workout 3
RECOV
CHO
*
Global RPE (15 min post-s)
DOMS
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Key point
Post workout multi-ingredients would improve recovery and the perception of effort after resistance training.
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Conclusion
The ingestion of protein-based supplements is a valid nutritional strategy for favouring optimal nutrition in athletes
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Dr. Fernando Naclerio
Programme Leader MSc Strength & Conditioning
[email protected] Fernando.naclerio.5 @FNaclerio http://www.fernandonaclerio.com
Thank you very much for your attention!