dna extraction
TRANSCRIPT
Extracting DNA from onions
Dr : Tahsin ShoalaEMAN Mohamed
-ALL OF US KNOW THAT “ DNA” , OR DEOXYRIBONUCLEIC ACID, IS THE HEREDITARY MATERIAL IN HUMANS AND OTHER ORGANISMS INCLUDING
PLANTS.
-MOST DNA IS LOCATED IN THE CELL NUCLEUS (WHERE IT IS CALLED NUCLEAR DNA) , BUT A SMALL AMOUNT OF DNA CAN ALSO BE FOUND IN THE MITOCHONDRIA (WHERE IT IS CALLED MITOCHONDRIAL DNA OR MTDNA).
Perface
DNA is surrounded by nuclear membrane , cell membrane and cell wall ,as well, in plant cells.
-I Observed that:
1 -DNA is found in the nucleus of membrane-bound cells. The membranes are lipid and protein in composition. It could be broken down using laundry detergent.
2 -DNA is a polymer made up of repeating chains of nucleotides. The sugar and phosphate components of DNA (the backbone) are both readily soluble in water.
Cont
3-The phosphate groups on the outside of DNA carry a negative charge. These negative charges are attracted to and are neutralized by cations such as sodium. When sodium is added to DNA it forms a protective "shell" around it. On the other hand, protein molecules precipitate from solution in the presence of salt.
4 -DNA is insoluble in ethanol (ethyl alcohol). As ethanol is added to a solution containing DNA, the DNA will come out of solution and stick to whatever is around.
Hypothesis
" If I could mix DNA with NaCl salt , Ethanol and laundary detergent Then i could extract
DNA and see it clearly“.
-And from this point I decided to conduct an experiment to extract the DNA from “Plant tissue”
-I choose “Onion” for my experiment as its cost , abundance and low starch content .
Apparatus
1 -Onions . 2 -Ethanol.
3 -Dish washing liquid . 4 -NaCl Salt.5 -test tubes .
6 -Beakers .7 -Water.
8-cutting board / funnel.Water bath. 9-
10 -knife.11 -blender.
procedure# I do procedure as indicated in the picture …
conclusion
I could extract DNA as indicated : Now