dna clean & concentrator

I DCC. Do you?

DCC?

A Guide toDNA Clean-up & Concentration

Featuring...The DNA Clean & Concentrator™ (DCC™):

The Most Comprehensive Solution for DNA Purification from Diverse Sources

2

DCC?Dallas

Cowboys Cheerleaders?

DNA Clean-up & Concentration Overview .................................................. 3-5Applications of Zymo Research's DNA Clean-up Technologies

High Quality DNA from PCR, Enzymatic Reactions & Other Sample Sources .. 6, 7Oligo Clean-up with COMPLETE Removal of Nucleotides & Salts ...................... 10Genomic & Large DNA Clean-up in Minutes ........................................................ 11ChIP DNA Isolation in 2 Minutes .......................................................................... 12Boost DNA Recoveries from Agarose Gels to >80% ............................................ 16Reusable DNA Sequencing Clean-up for Long, High-quality Reads ................... 18DNA Too Dirty for PCR? Clean it with OneStep! .................................................. 19

SpotlightQuick, Simple cDNA Clean-up with the DCC™ Following Reverse-transcription .... 9

DNA Clean-up FAQs ....................................................................................... 14Ordering Information ................................................................................ 20, 21DNA Clean-up Guide ...................................................................................... 22

www.zymoresearch.com 3

DNA Clean-up from Diverse Sample Sources

Ideal For...

Next-Gen & Sanger SequencingTransfection

TransformationDNA Ligation

CloningArrays

Enzymatic Digestion/Modification

SpinWash Elute

Ultra-Pure DNA

PCR, Enzymatic Reactions, Desalting, and Other Routine Clean-up Removal of enzymes, primers, dyes, nucleotides, salts, etc.

Genomic DNA Clean-up DNA purification from any impure preparation.

Gel DNA RecoveryDNA from TBE and TAE buffered agarose gels.

Oligo Clean-upPurification of oligonucleotides with the complete removal of dNTPs, and salts.

Sequencing Reaction CleanupRemoval of enzymes, nonincorporated dyes, primers, dNTPs, and salts.

PCR Inhibitor RemovalRemoval of polyphenolic compounds including melanin, humic acid, fulvic acid, and others.

4 www.zymoresearch.com

Use the Right Tool for the Job!

Zymo’s Innovative Column Design Ensures...

6 μl minimal elution

0 μl wash residue carryover

Weighed Down by Impure DNA?

PCR efficiency can be limited by excess salts such as guanidinium, KCl, and NaCl, ionic detergents including sodium deoxycholate, sarkosyl, SDS, and alcohols like ethanol, isopropanol, and phenol. These PCR inhibitors usually affect amplification through interaction with DNA or interference with the DNA polymerase. Also, polyphenolic substances (e.g., melanin & humic acid) from impure preparations can chelate Mg2+ and inhibit PCR. Many commercial products available today result in some amount of inhibitor carryover.

Purposeful in design and robust in function, the scientists at Zymo Research have pioneered the first low elution Zymo-Spin™ column. This innovative spin column allows purification of up to 5 µg of DNA in as little as 6 µl eluate with no buffer retention or carryover. Manufactured exclusively by Zymo Research Corp. Accept no imitations!

Zymo-Spin™ IC shown, Cat. No. C1004-50, C1004-250


Question Your Spin Column!

VS.

MinElute® Spin column

2-3 μl Carryover!

Zymo-Spin™ Column

Micro Elution Tip

Zymo-Spin™ Columns Ensure No Buffer Retention

Unleash the BEST DNA!

High quality, inhibitor-free DNA is crucial to ensure success with qPCR, DNA ligation/cloning, sequencing, arrays, etc. The scientists at Zymo Research have developed the most comprehensive technologies for DNA clean-up and concentration from any preparation. Core to these products is the total removal of salts/alcohol from samples with uniquely designed spin columns and plates that ensure complete elution with no binding/wash buffer carryover. Coupled with uniquely formulated buffers these technologies assure the purification of high quality DNA without the inclusion of inhibitors.

Inhibitors may...

Be present in the original sample, such as tissues, blood, soil, plants, etc.

Result from inadequate removal during DNA isolation

Be introduced by the DNA extraction technique used


High Quality DNA from PCR, Enzymatic Reactions & Other Sample Sources

6 μl minimal elution with 0 wash residue carryover!

Product Binding Capacity

Elution Volume Preps Cat. #

DNA Clean & Concentrator™-5 (uncapped) 5 µg / prep. ≥ 6 µl

50 D4003

200 D4004

DNA Clean & Concentrator™-5 (capped) 5 µg / prep. ≥ 6 µl

50 D4013

200 D4014

ZR-96 DNA Clean & Concentrator™-5 5 µg / well ≥ 10 µl2 x 96 D4023

4 x 96 D4024

Highlights

Quick, 2 minute recovery of ultra-pure DNA that is free of salts and contaminants.

DNA can be eluted in as little as 6 µl and is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transformation, restriction digestion, etc.

DescriptionThe DNA Clean & Concentrator™-5 (DCC™-5) and ZR-96 Clean & Concentrator™-5 (ZR-96 DCC™-5) offer reliable methods for DNA purification from PCR, restriction digests, RT reactions, and other impure preparations. They can be used for the efficient removal of DNA polymerases, modifying enzymes, dyes, salts, dNTPs, etc.

Pure & Consistent DNA Recovery with the DCC™-5. Eluted DNA was analyzed using a Nanodrop® spectrophotometer. Spectra show DNA to be highly pure with consistent recoveries of >90% of input.

DNA amplicons can be efficiently purified and concentrated using the DCC™.

MOriginal

PCRPurified

PCR

600

300200

100

PCRProduct

Primers High efficiency DNA recovery using the DCC™-5. Equivalent amounts of DNA were purified using the DCC™-5 or MinElute® Kit and analyzed by electrophoresis in a 0.8% (w/v) agarose/TAE/EtBr gel.

M

3 kb

2 kb1.5 kb

1 kb

DCC™ MinElute®

7

I DNA Clean & Concentratein a 6 µl eluate.



DNA Clean & Concentrator™-25 (uncapped) 25 µg / prep. ≥ 25 µl

50 D4005

200 D4006

DNA Clean & Concentrator™-25 (capped) 25 µg / prep. ≥ 25 µl

50 D4033

200 D4034

DNA Clean & Concentrator™-100 100 µg / prep. ≥ 150 µl25 D4029

50 D4030

DNA Clean & Concentrator™-500 500 µg / prep. ≥ 2 ml

10 D4031

20 D4032

Other Available Formats...

Did You Know? The DNA Clean & Concentrator™ is recommended in Illumina’s Nextera™ Sample Prep and Infinium® HD FFPE Restore workflows.

Many DNA isolation methods include buffers containing salts and ionic detergents used during purification. If improperly removed, these compounds can hinder highly sensitive DNA analyses including Next-Gen sequencing, arrays, and qPCR.

Zymo Research's Zymo-Spin™ technology is designed to ensure no buffer carryover, making the DNA Clean & Concentrator™ ideal for DNA clean-up prior to Next-Gen sequencing.

8

Will you DCC with me?

In a lab or in a cab?

For PCR purification

or cDNA isolation.

For cleaning up impure

preparations and

For excellent ligations.

Featured DCC™ Citations

1. Li, Ning et al. (2010) Whole genome DNA methylation analysis based on high throughput sequencing technology. Methods, 52 (3), 221-232.

2. Khoo, Sok et al. (2011) Acquiring genome-wide gene expression profiles in Guthrie card blood spots using microarrays. Pathology, 61 (1), 1-6.

3. Lee, Eun-Joon et al. (2011) Targeted bisulfite sequencing by solution selection and massively parallel sequencing. Nucleic Acids Research, 39(19), e127, doi:10.1093/nar/gkr598.

4. Ferguson, Annabel A. et al. (2009) Retrofitting ampicillin resistant vectors by recombination for use in generating C. elegans transgenic animals by bombardment. Plasmid, 62, 140-145.

5. Wieland, Markus et al. (2009) Investigation of mRNA quadruplex formation in E.coli. Nature Protocols, 4, 1632-1640.

6. Hedges, Dale et al. (2009) Exome sequencing of a multigenerational human pedigree. PLos ONE, 4 (12), e8232.

7. Mehta, Manan M. et al. (2012) A transposase strategy for creating libraries of circularly permuted proteins. Nucleic Acids Research, 1-8, doi: 10.1093/nar/gks060.

8. Cho, M. et al. (2010) Quantitative selection of DNA aptamers through microfluidic selection and high-throughput sequencing. PNAS, 107 (35), 15373-15378.

9. Bi, W. et al (2008) Rapid prenatal diagnosis using uncultured amniocytes and oligonucleotide array CGH. Prenatal Diagnosis, 28 (10), 943-949.

10. Malik, Saafan Z. et al. (2011) Identification of potentially neuroprotective genes upregulated by neurotrophin treatment of ca3 nuerons in the injured brain. Journal of Neurotrauma, 28 (3), 415-430.


Quick, Simple cDNA Clean-up with the DCC™ Following Reverse-transcription

The DCC™ kit can be used to effectively clean and concentrate first-strand cDNA following reverse-transcription (RT) in the presence/absence of fluorescent dyes. Unincorporated free nucleotides and fluorescent derivatives are efficiently removed using the DCC™, and the recovered cDNA may be used directly for microarray analysis, second-strand cDNA synthesis, or indirect labeling with a fluorescent dye such as NHS-ester Cy3 or Cy5.

The protocol below is for the purification of first-strand cDNA following RT using the DCC™.

A. Hydrolysis● Add 10 μl 0.5 M EDTA and 10 μl 1 N NaOH to 40-50 μl of an RT

reaction.

● Incubate at 65°C for 15 minutes.

B. Clean-up with the DCC (or Oligo Clean & Concentrator™)*

● Add 490 μl (7 volumes) of DNA Binding Buffer to the hydrolysis reaction above.Note: Neutralization following the hydrolysis reaction is not necessary. The DNA Binding Buffer can effectively neutralize the NaOH in the reaction.

● Mix well.

● Load the sample to a provided Zymo-Spin™ Column in a Collection Tube. Centrifuge for 30 seconds. Discard the flow-through.

● Add 200 μl DNA Wash Buffer and spin 30 seconds. Add another 200 μl DNA Wash Buffer and spin for an additional minute.

● Add 10 μl water to the column, place the column into a new tube, wait for one minute. Elute the cDNA by brief centrifugation. Note: Modified TE (10 mM Tris-HCl, pH 8.5, 0.1 mM EDTA) or 0.1 M sodium bicarbonate, pH 9.0 can also be used for elution.

* Alternatively, for short cDNAs >16 nt [e.g., expressed sequence tags (EST)] use the Oligo Clean & Concentrator™ (see page 10) in conjunction with the protocol above.

SPOTLIGHT

Did You Know? NuGen recommends Zymo Research's DNA Clean & Concentrator™-25 (Cat. No. D4005) for purification of amplified cDNA with their WT-Ovation™ Pico RNA Amplification System.


Highlights

Quick (2 minute) recovery of ultra-pure DNA oligonucleotides with complete removal of enzymes, nucleotides, dyes, and salts.

Zero retention spin columns with minimal (≥6 µl) elution volumes.

Eluted DNA is well suited for use in PCR, probe hybridization, sequencing, ligation, etc.

DescriptionThe Oligo Clean & Concentrator™ provides a streamlined method for efficient recovery and clean-up of oligonucletides (≥16 nt) from labeling (radioactive, biotin, DIG, etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos (<6 mer), dyes, enzymes, and salts are effectively removed by the clean-up procedure.

Oligo Clean-up with COMPLETE Removal of Nucleotides & Salts

The Oligo Clean & Concentrator™ facilitates >90% recovery of ssDNA oligonucleotides.

60 mer40 mer30 mer20 mer16 mer0.0

20.0

40.0

60.0

80.0

100.0

Per

cent

(%) R

ecov

ery

Oligo Clean & Concentrator™

Supplier Q



Oligo Clean & Concentrator™ 10 µg / prep. ≥ 6 µl50 D4060

200 D4061

ZR-96 Oligo Clean & Concentrator™ 10 µg / prep. ≥ 10 µl2 x 96 D4062

4 x 96 D4063

Works with RNA Too!


Did You Know?

Zymo Research provides the only spin columns optimized for binding and eluting genomic and large DNAs.

The image to the right is an electron micrograph showing the uniform porosity of the spin column matrix featured in the Genomic DNA Clean & Concentrator™. This unique binding matrix facilitates maximum recovery of high molecular weight DNAs (up to 10 μg into as little as 10 μl eluate).

Illumina recommends the Genomic DNA Clean & Concentrator™ be used to ensure DNA quality prior to input into the Nextera™ DNA Sample Prep Kit.

Genomic & Large DNA Clean-up in Minutes

High molecular weight DNA is efficiently purified using the Genomic DCC™. Porcine gDNA (~35-50 kb), T4 phage DNA (170 kb), and lambda phage DNA (48.5 kb) were purified using the Genomic DCC™. Samples are resolved in a 0.8% TAE/agarose/EtBr gel. The size marker “M” is a 1 kb ladder (Zymo Research).

Lambda phage DNA (48.5 kb) is effectively recovered from various concentrations of starting material using the Genomic DCC™.



Genomic DNA Clean & Concentrator™ 10 µg / prep. ≥ 10 µl25 D4010

100 D4011

Highlights

Perfect for genomic and large DNA clean-up from impure preparations.

Low volume elution (≥10 µl) of ultra-pure DNA in 5 min.

Eluted DNA is ideal for PCR, restriction endonuclease digestion, Sanger and Next-Gen sequencing.

DescriptionUnlike other columns that can physically trap and shear large DNAs [e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga) DNA, etc.], Zymo Research has developed a unique spin column that is featured in the Genomic DNA Clean & Concentrator™. The Genomic DCC™ allows recovery of ultra-pure, large-sized (100 bp to ≥200 kb) DNA from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion).




ChIP DNA Clean & Concentrator™ (uncapped) 5 µg / prep. ≥ 6 µl 50 D5201

ChIP DNA Clean & Concentrator™ (capped) 5 µg / prep. ≥ 6 µl 50 D5205

ZR-96 ChIP DNA Clean & Concentrator™ 5 µg / well ≥ 10 µl2 x 96 D5206

4 x 96 D5207

Highlights

High detergent tolerance (≤1% SDS or ≤5% sarkosyl).

Minimal elution volumes (≥6 μl) for highly concentrated DNA.

Eluted DNA is ideal for PCR, arrays, ChIP-Seq, and other highly sensitive molecular applications.

DescriptionThe Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator™ provides a hassle-free method for the rapid purification and concentration of high quality DNA from any step in a standard ChIP protocol. This includes samples that have undergone reverse cross-linking, Proteinase K or RNase A digestion, mechanical or nuclease-mediated DNA shearing, and samples eluted from chromatin-antibody-bead complexes.

ChIP DNA Isolation in 2 Minutes

Quantitative recovery of DNA from cell lysates using the ChIP DCC™. Purification of 5, 15, and 30 µl yeast cell lysate. Analysis of eluted DNA by Nanodrop spectrometry demonstrates that the amount of DNA recovered is proportional to the lysate volume.

350300250200150100

500

Con

cent

ratio

n (n

g/µl

)

Cell Lysate (µl)5 15 30

Yeast ChIP PCR Analysis. Galactose (GAL) genes, GAL 7 and 10, were induced in Saccharomyces cerevisiae. A ChIP assay was performed using an antibody specific for RNA polymerase II. DNA was purified by ChIP DCC™ following reverse cross-linking and Proteinase K digestion. PCR products were subsequently analyzed by agarose gel electrophoresis.

GAL 7

Galactose Induction- + - +

GAL 10

Try it and you may see how wonderful it is - The DCC™!

13

Did You Know?

DNA fragmented with a sonicator or nebulizer is purified using the DNA Clean & Concentrator™-25 prior to Roche Nimblegen’s Sequence Capture Arrays (2.1 M and 385 K arrays).

Watch the DCC™ Video!

14

DNA Clean-up FAQs

Q: What is the lowest amount of DNA I can purify using the DCC™-5?A: The DCC™-5 is able to recover <1 ng DNA.

Q: What is the smallest sample volume I can use with the DCC™-5?A: There is no lower limit. For samples volumes less than 50 μl adjust volume with water to a final volume of 50 μl, then add DNA binding buffer as recommened in the protocol.

Q: I have a large sample volume, can the spin column be reloaded?A: The column has a 700 μl capacity and can be reloaded 2-3 times as needed.

Q: For DNA clean-up, can I use Zymo's columns with Qiagen’s buffers?A: Yes, Zymo-Spin columns are compatible with Qiagen’s buffer systems.

(Ordering information is on page 19.)

The Zymo-Spin™ IC columns are compatible with Qiagen's MinElute® buffer system. 2 µg of a 50 bp ladder was isolated using the MinElute® kit or with the MinElute® kit with Zymo-Spin™ IC columns. Equal volumes of eluate were analyzed by electrophoresis in a 2% agarose gel stained with ethidium bromide.

Q: What can I use to elute my DNA from the spin column?A: DNA can be eluted with Zymo Research's DNA Elution Buffer (10 mM Tris-HCl, pH 8.5,

0.1 mM EDTA), TE, or water.

Did You Know?

If samples seem degraded when resolved in an agarose gel, this may be due to acidification of the 6X loading dye. Loading dyes are a rich carbon source (sucrose, glycerol, etc.) that can sustain microbial growth resulting in acidification over time. This can cause the appearance of sub-bands in DNA. Therefore, it is recommended to supplement loading dyes with 1 mM EDTA to prevent microbial growth and acidification.

MinElute® Column Zymo-Spin™ ICC

15

“This kit is the best product I ever had. Comparing to Qiagen, this kit is more efficient, easier and faster to use.”

M.K., Technical University of Lodz, Institute of Technical Biochemistry

“Zymo Kits blew me away, they work like a charm. I LOVE THEM!!!”P.K., Western Michigian University

“It was very easy to use. My inexperienced student was able to do it without guidance.”P.W., University of Pittsburg, Cell Biology

“It worked. PCR inhibitors were gone.”S.J., MLL GmbH, Molekulargenetik

“It is easy to use. And the yield is quite high.”H.G., Emory University, Anesthesiology

They DCC... Do you?

This is great, this DCC™.

Now I'll take it all for me!


DNA fragments recovered from an agarose gel using the Zymoclean™ Gel DNA Recovery Kit. Lanes: M: DNA Ladder; 1-5: individual ladder DNA fragments.

M 1 2 3 4 5

23 kb9 kb

2 kb

500 bp

Blunt-ended ligation of DNA fragments purified using the Zymoclean™ Large Fragment DNA Recovery Kit. Fragments from plasmid DNA digested with PvuII were purified, then mixed and ligated for the times indicated in the figure (above).

Pvulldigest

Recovered byZymoclean

Blunt-EndLigation

3.0 kb -

330 bp -



Zymoclean™ Gel DNA Recovery Kit (uncapped) 5 µg / prep. ≥ 6 µl

50 D4001

200 D4002

Zymoclean™ Gel DNA Recovery Kit (capped) 5 µg / prep. ≥ 6 µl

50 D4007

200 D4008

ZR-96 Zymoclean™ Gel DNA Recovery Kit 5 µg / well. ≥ 15 µl

2 x 96 D4021

4 x 96 D4022

There’s a Zymoclean™ for Large (>20 kb) DNAs too!

Zymoclean™ Large Fragment DNA Recovery Kit 10 µg / prep. ≥ 10 µl

25 D4045

100 D4046

Highlights

Rapid (15 minute) recovery of ultra-pure DNA from agarose gels.

Unique column design permits DNA elution into minimal volumes (≥ 6 μl).

Eluted, ultra-pure DNA is ideal for direct use in all applications such as DNA ligation, sequencing, labeling, PCR, microarray, transformation, restriction digestion, etc.

DescriptionThe Zymoclean™ Gel DNA Recovery Kit raises the bar in DNA purification from TAE or TBE-buffered agarose gel slices. Coupling robust agarose dissolving technology with efficient spin column adsorption of DNA, the Zymoclean™ Gel DNA Recovery Kit obtains the highest DNA yields commercially available.

Boost DNA Recoveries from Agarose Gels to >80%


Did You Know? Most commercially available DNA gel extraction kits recover only 20-40% of input DNA.

Curious if yours falls into this category? Compare what you’re currently using with the Zymoclean™ Gel DNA Recovery Kit. Order a sample for evaluation today!

Ensuring Optimal Gel DNA Recovery with Zymoclean™

1 AgaroseZymoclean™ is compatible with both low-melting and general purpose TAE- and TBE-buffered agarose gels. Make sure to trim as much excess agarose from the excised DNA as possible. Avoid processing more than 400 mg of total agarose.

2

3

Fully Dissolve AgaroseEnsure agarose is fully dissolved in ADB prior to loading into a spin column. Undissolved agarose may clog the spin columns, inhibiting DNA recovery.

4

Dissolving TemperatureDissolve agarose gel slices between 37o-55oC for optimal results. If dissolved at a higher temperature (above 60oC) DNA is denatured which may affect recovery.

DNA ElutionDNA can be eluted with Zymo Research's DNA Elution Buffer (10 mM Tris-HCl, pH 8.5, 0.1 mM EDTA), TE, or water. If using water, ensure the pH is ≥6.0.


Reusable DNA Sequencing Clean-up for Long, High-quality Reads

Sequencing chromatogram of pGEM® DNA generated using an ABI 3730XL DNA Analyzer® with or without DNA clean-up using ZR DNA Sequencing Clean-up Kit™. DNA was labeled with ABI BigDye® v3.1 Terminators and cleaned using the ZR DNA Sequencing Clean-up Kit™.

ZR DNASequencingClean-up Kit

No Clean-up



ZR DNA Sequencing Clean-up Kit™ 5 µg / prep. ≥ 6 µl

50 D4050

200 D4051

ZR-96 DNA Sequencing Clean-up Kit™ 5 µg / well ≥ 15 µl2 x 96 D4052

4 x 96 D4053

Highlights

Rapid (2 minute) recovery of ultra-pure DNA from post-cycle sequencing reactions.

Unique column design permits minimal elution volumes (≥ 6 μl) for highly concentrated DNA. No more time consuming precipitations or size exclusion resins.

Eluted DNA is ready for dye blob-free sequencing.

DescriptionThe ZR DNA Sequencing Clean-up Kit™ provides the simplest method for rapid removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants often interfere with the quality and signal strength of sequencing data. In particular, unincorporated dyes can result in dye peaks (“dye blobs”) which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software.




OneStep™ PCR Inhibitor Removal Kit Variable 50 - 200 µl 50 D6030

OneStep - 96™ PCR Inhibitor Removal Kit Variable 50 - 100 µl 2 x 96 D6035

Add Sample

OneStep™ PCR Inhibitor Removal Kit Procedure

Inhibitor-Free DNA (or RNA)

$

Highlights

For high quality DNA that is free of enzymatic inhibitors including polyphenolics, humic/fulvic acids, tannins, melanin, etc.

Fast, one-step procedure for cleaning impure DNA samples prior to PCR, sequencing, RT, etc.

DescriptionThe OneStep™ PCR Inhibitor Removal Kit is a truly novel product that provides a single step method for elimination of contaminants in DNA/RNA preparations that inhibit downstream enzymatic reactions such as PCR and RT. Cleanup is simple! Just apply, spin, and elute a sample from the column.

DNA is efficiently amplified by PCR following humic acid removal with the OneStep™ PCR Inhibitor Removal Kit. Equivalent amounts of DNA were analyzed in a 2.0% (w/v) agarose/TAE/EtBr gel. The ladder is a 100 bp DNA marker (Zymo Research). PCR was performed using ZymoTaq™ PreMix (Zymo Research).

Ladder Controls Non-Treated Treated300 bp

200 bp

100 bp

Product

DNA Too Dirty for PCR? Clean it with OneStep!

Works with RNA Too!


Ordering Information

Product Description Preps Cat. No.

DNA Clean-up

DNA Clean & Concentrator™-5

Clean and concentrate up to 5 µg DNA into ≥6 µl elution volume in as little as 2 minutes with no wash residue carryover.

5020050

200

D4003 (uncapped)D4004 (uncapped)D4013 (capped)D4014 (capped)

DNA Clean & Concentrator™-25

Clean & concentrate 25 µg of DNA into ≥25 µl elution volume in as little as 2 minutes with no wash residue carryover.

5020050

200


ZR-96 DNA Clean & Concentrator™-5

Quick (15 minute), high-throughput recovery of up to 5 µg pure DNA into 10-15 µl minimum elution volume allows for highly concentrated DNA.

2 x 964 x 96

D4023D4024

Genomic DNA Clean & Concentrator™

Quick (5 minute) clean-up of up to 10 µg high molecular weight DNA (≥200 kb) from any enzymatic reaction or impure preparation without precipitations.

25100

D4010 (capped)D4011 (capped)

Zymoclean™ Gel DNA Recovery Kit

Purify DNA from high and low-melting agarose gels in minutes.

5020050

200


ZR-96 Zymoclean™ Gel DNA Recovery

Kit

High-throughput DNA purification from high and low-melting agarose gels.

2 x 964 x 96

D4021D4022

Zymoclean™ Large Fragment DNA Recovery Kit

Purify high molecular weight DNA (≥20 kb) from high and low-melting agarose gels in minutes.

25100

D4045 (capped)D4046 (capped)

OneStep™ PCR Inhibitor Removal Kit

Fast, one-step procedure for removal of PCR inhibitors such as polyphenolics, humic/fulvic acids, melanin, etc. for successful PCR and other downstream applications.

502 x 96

D6030D6035

ZR DNA Sequencing Clean-up Kit ™

Simplest, most reliable method for dye-terminator removal from DNA.

50200

D4050 D4051

ZR-96 DNA Sequencing Clean-

up Kit ™

Simplest, most reliable method for dye-terminator removal from DNA.

2 x 964 x 96

D4052D4053

Featured Columns & Plates

Zymo-Spin™ I The Zymo-Spin™ I columns can be used either in microcentrifuges or on vacuum manifolds for the purification of DNA and/or RNA. Up to 5 µg DNA or RNA can be eluted in ≥ 6 µl. Capacity is 800 µl.

50250

C1003-50C1003-250

Zymo-Spin™ IC 50250

C1004-50C1004-250

Zymo-Spin™ I-96 Plate

The Zymo-Spin I-96™ Plate can be used in centrifuges for the large-scale (i.e., 96-well) purification of DNA and/or RNA. Up to 5 μg DNA or RNA can be eluted in ≥ 10 μl per well. Capacity is 1.1 ml per well.

2 plates C2004

For other column and plate technologies, please visit www.zymoresearch.com

Plasmid DNA Purification

Zyppy™

Plasmid Miniprep KitPellet-Free™ plasmid DNA purification in less than 10 minutes. Recover up to 25 µg DNA in as little as 30 µl.

50100400800

D4036D4019D4020D4037

Zyppy™-96 Plasmid Miniprep

The fastest and simplest high-throughput method for plasmid purification.

2 x 964 x 968 x 96

D4041D4042D4043

Zyppy™ -96 Plasmid MagBead Miniprep High-throughput method for automated plasmid purification.

2 x 964 x 968 x 96

D4100D4101D4102

Zyppy™

Plasmid Midiprep KitPellet-Free™ plasmid DNA purification in 15 minutes in a 150 µl minimum elution volume.

2550

D4025D4026

ZR Plasmid MiniPrep™-Classic

Plasmid DNA purification in minutes: (alkaline lysis/spin column format for low 30 µl elution volume).

100400800

D4015D4016D4054

Zyppy™ Plasmid Maxiprep Kit

A unique method for spin-column purification of high quality, endotoxin-free plasmid DNA in minutes.

1020

D4027D4028


Product Description Preps Cat. No.

Genomic DNA Purification

Quick-gDNA™ MiniPrep

Easy purification of genomic DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, tissue, swabs or cultured cells in as little as 15 minutes without the use of Proteinase K or organic denaturants.

5020050

200


ZR-96 Quick-gDNA™Simple, high-throughput (96-well) purification of DNA from whole blood, plasma, serum, body fluids, buffy coat, lympho-cytes, tissue, swabs, or cultured cells in about 30 minutes.

2 x 964 x 96

10 x 96

D3010D3011D3012

ZR-Genomic DNA™-Tissue MiniPrep

For high quality DNA purification from solid tissues (e.g., tail snips, ear punches, adipose tissue, etc.), body fluids, cultured cells, buccal cells, FFPE tissues, hair, and other biological sources using Proteinase K.

50200

D3050D3051

Environmental DNA Purification Kits

Unique BashingBead™ technology allows isolation of DNA from samples refractory to conventional lysis procedures including tough-to-lyse tissues, soil samples, feces, plants, seeds, insects, bacteria, yeast, filamentous fungi, unicellular and filamentous algae, and protozoa.

Please visit www.zymoresearch.com

RNA Purification

RNA Clean & Concentrator™-5

Clean and concentrate up to 5 µg RNA into ≥6 µl elution volume in as little as 5 minutes with no wash residue carryover.

50200

R1015 R1016


Clean & concentrate 25 µg of RNA into ≥25 µl elution volume in as little as 5 minutes with no wash residue carryover.

50100

R1017R1018


Clean & concentrate 125 µg of RNA into ≥100 µl elution volume in as little as 10 minutes with no wash residue carryover.

25 R1019

ZR-96 RNA Clean & Concentrator™

Simple, high-throughput recovery of up to 25 µg pure RNA into 10 µl minimum elution volume allows for highly concentrated DNA.

2 x 96 R1080

Direct-zol™ RNA MiniPrep

Quick (10 minute), spin column purification of up to 50 µg into 25 µl high-quality total RNA directly from TRI-Reagent®, TRIzol®, or similar.

5020050

200

R2050R2052 R2051 (w/ TRI-Reagent®)

R2053 (w/ TRI-Reagent®)

Quick-RNA™ MicroPrep

Easy purification of total RNA from cultured cells (1 to 105) and solid tissue samples in as little as 10 minutes without the use of Proteinase K or organic denaturants.

50200

R1050R1051

Quick-RNA™ MiniPrep


50200

R1054R1055

Quick-RNA™ MidiPrep


25 R1056

ZR-96 Quick-RNA™ Simple, high-throughput (96-well) purification of RNA from a wide range of cell types and tissue in about 30 minutes.

2 x 964 x 96

R1052R1053

™ - Trademarks of Zymo Research Corporation. Nextera™ and Infinium® are trademarks of Illumina, Inc. MinElute® is a trademark of Qiagen, GmbH. WT-Ovation™ Pico RNA Amplification System is a trademark of NuGen, Inc. TRI-Reagent® and TRIzol® are registered trademarks of Molecular Research Center, Inc.

100% Satisfaction Guaranteed

Zymo Research is committed to the highest standard of quality and assures your satisfaction with its products.

ISO 9001:2008 Certified

EpigeneticsTHE

COMPANY™

Since 1994, Zymo Research has been offering innovative, quality, and easy-to-use tools for DNA/RNA purification. Now, as “The Epigenetics Company”, Zymo Research offers a wide range of products and services for epigenetics research. From industry leading bisulfite technologies to state of the art services for genome-wide DNA methylation and hydroxymethylation analysis, Zymo Research provides the most consolidated and regarded products in the industry.


DN

A C

lean-u

p G

uid

e

DN

A from

ChIP

DN

A from...

PC

R,

Restriction D

igests,R

everse Transcriptions,P

roteinase K D

igests,D

ye Labeling,A

ny Cell-free R

eactions/Lysates

PC

R Inhibitor

Rem

oval Including...P

olyphenolicsH

umic A

cidsFulvic A

cidsTanninsM

elanin

Sequencing R

eaction C

lean-up

Genom

ic DN

A C

lean &

Concentrator ™

Oligo

Clean &

C

oncentrator ™

Zymoclean

Gel D

NA

Recovery K

it ™

Large Fragment

Zymoclean

Gel D

NA

Recovery K

it ™

PC

R, C

hIP-S

eq, Arrays,

Next-G

en sequencing, etc.S

equencing

ChIP D

NA

Clean &

Concentrator ™

OneS

tep™

PC

R Inhibitor

Rem

oval Kit

ZR D

NA

Sequencing

Clean-up K

it ™

PC

R, Ligation, Transfection, Transform

ation, Sanger &

Next-G

en Sequencing, R

estriction Digests, A

rrays, Labeling &

Other D

NA M

odifications

DN

A

Clean &

C

oncentrator ™

DN

A fromA

garose Gels

gDN

A from

Impure

Preparations

Oligo

Clean-up


USA: (888) 882-9682INTL: (949) 679-1190Fax: (949) 266-9452

Your questions, comments, and suggestions are always [email protected]

Please recycle this newsletter

Find us on Facebook & follow us on Twitter for the latest product releases, special offers, and more!

NEW! Lab Essentials Set

Cat. No. D4000-LE

The Lab Essentials Set Includes

DNA Clean & Concentrator™ (DCC™) Zyppy™ Plasmid MiniPrep Zymoclean™ Gel DNA Recovery Kit

Buy the Set & Save!

17062 Murphy Ave.

Irvine, CA 92614, U

SA

I DC

C. D

o y

ou?

dna clean & concentrator

Documents

dna purification

impure dna

genomic dna

dna polymerase

g of dna

reusable dna sequencing

genomic large dna

technologieshigh quality