SpeedVac SPD1010 ConcentratorJiarong (JR) ZhouInjectables

IntroductionUV-Vis SpectrophotometerVerification of SpeedVac SPD1010 ConcentratorO UV-Vis as Analytical

InstrumentNew EquipmentO Functionality?O Applications?O Limits?

SpeedVac SPD1010 Concentrator

CentrifugeO Prevents bumpingVacuum PumpO Creates a vacuumThermal HeatO ConvectionRadiant Cover (RC)O Heats by radiationRefrigerated Vapor TrapO -50°C

ParametersPreHeatO 45°CRC On/OffTemperatureO 45°C ~ 80°C (±5°C)O No HeatHeat/Run TimeVacuum PressureO 5.1 torr ~ 30.0 torr (±0.1 torr)Vacuum Ramp RateO 1 ~ 5

Purpose & ProceduresReplicating a Possible ScenarioAspirin in 50/50 H2O/MeOHAnalytical λO Calibration curvePrepared samples below LOQO 0.4µg/mL Aspirin in 50/50 H2O/MeOHMethod DevelopmentO Bumping/FoamingO Over DryingO Denaturing

5mg/mL

0.5mg/mL

0.05mg/mL

Calibration Curve

0.00 0.05 0.10 0.15 0.20 0.250.00

0.20

0.40

0.60

0.80

1.00

1.20

f(x) = 5.68948717948718 x − 0.0281391025641026R² = 0.999809070143822

Aspirin in 50/50 H2O/MeOH

Concentration (mg/mL)

Abso

rban

ce

0.4µg/mL

Partially Concentrated & Combined

Concentrated & Reconstituted with 1mL Solvent

Absorbance (AU) 0.0020 0.0393 0.0142

Concentration (µg/mL) <LOQ 11.846 7.435

ResultsLoss of Sample!O Bumping/FoamingO Uneven drying?1.5mL Samples

It Works!O Able to dry/concentrate samplesQualitative vs Quantitative

ReferencesRoger ParksAnalytical Chemistry 2.0DailyMed – Bayer Aspirin TabletGenevac – All About BumpingIntegrated SpeedVac® System SPD1010/SPD2010 Instruction ManualThermo Scientific Savant SPD1010 and SPD2010 SpeedVac® ConcentratorsValidation of Analytical Procedures: Text and Methodology

Questions?