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CORE FACILITIES AND OTHER SERVICES AVAILABLE ON THE ANSCHUTZ MEDICAL CAMPUS AND AFFILIATED SITES

Prepared by the Department of Medicine Research Office

Last updated 8/20/2015

For corrections, additions, updates, or to report broken links please contact:

Sheryl Hartmann sheryl.hartmann@ucdenver.edu

303-724-1786

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CONTENTS

ACCORDS: Adult and Child Center for Health Outcomes Research and Delivery Service Qualitative Core Research Training Program Dissemination and Implementation Program (CRISP) Education Program Community Engagement and Outreach Practice-Based Research Network (PBRN) Health Informatics and m-Health Biostatistics and Analysis

Altitude Research Center (ARC)

Animal Care and Use Program Animal Care and Use Program (IACUC) Office of Laboratory Animal Resources (OLAR)

Barbara Davis Center for Diabetes Service Centers Autoantibody/HLA Flow Cytometry Islet

CardioVascular BioImaging Core Vascular Imaging Cardiac Imaging Technique Development Educational Services Colorado Genetics Laboratory Prenatal Testing Postnatal Testing Cancer Testing Tissue Testing Fluorescence in situ Hybridization (FISH) Chromosomal Microarray

Center for Advancing Professional Excellence (CAPE)

Center for NeuroScience (CNS) and Rocky Mountain Neurological Disorders (RMND) Microscopy Core (Core A) - RMND

Advanced Light Microscopy Core Facility Electron Microscopy Center

Targeting Vector Core (Core B) - RMND Zebrafish Transgenic Core (Core C) - RMND Machine Shop (Core D) - RMND Animal Behavior Core - CNS Real Time QPCR Core - CNS Optogenetics Core - CNS

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Center for Surgical Innovation

ClinImmune Labs Colorado Cord Blood Bank Stem Cell Lab Flow Cytometry Histocompatability

Colorado Biostatistics Consortium (CBC) in the Colorado School of Public Health

Colorado Clinical and Translational Sciences Institute (CCTSI) Network of Translational Technologies (NeTT) Biostatistics, Epidemiology, & Research Design (BERD) Child & Maternal Health Clinical & Translational Research Centers (CTRC) Colorado Profiles Pragmatic Trials & Dissemination/Implementation Research Regulatory Knowledge & Support Research Ethics Consult Service Research Studio Program Translational Informatics Education, Training, & Career Development (ETCD)

Colorado Translational Research Imaging Center (C-TRIC) in the Department of Radiology

Denver Genetic Laboratories

Department of Pathology Autopsy Colorado Molecular Correlates Lab (CMOCO) Cytology Clinical Lab Histology Prostate Diagnostic Lab Surgical Pathology

Environmental Health and Safety

The Evaluation Center

Gates Center for Regenerative Medicine Histology (Morphology and Phenotyping) Core iPSC Core Transgenics and Gene Targeting Core Molecular Genetics Analysis Core Gates Biomanufacturing Facility

Health Sciences Library

JFK Partners

Office of Grants and Contracts

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Skaggs School of Pharmacy Core Facilities Mass Spectrometry Medicinal Chemistry High Throughput and High Content Screening Core Facility Computational Chemistry and Biology Core Facility

Statistical Consulting Services in the College of Liberal Arts and Sciences

Structural Biology and Biochemistry Program Core Facilities Biophysics Computational Biology NMR Structural Biology Facility Peptide and Protein Chemistry Facility Xray Crystallography Structural Biology Shared Resource at CU Cancer Center Biological Mass Spectrometry Facility

Proteomic Services Metabolomic Services

Systems Biology Core Facility

University of Colorado Cancer Center Biostatistics & Bioinformatics Cancer Clinical Trials Office Small Animal Imaging Service Flow Cytometry Functional Genomics Genomics and Microarray Human Tumor Model-Based Databank Molecular Pathology

Cytogenetics Preanalytical Evaluation & Molecular Testing Services

Pharmacology Protein Production/MoAB/Tissue Culture Research Informatics Tissue Biobanking & Processing

Biorepository Service Laser Capture Microdissection Service

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Barbara Davis Center for Diabetes Service Centers

Description: Our Research Division offers four different Service Centers: Autoantibody/HLA Service Center, Cytometry Service Center, Islet Service Center, and Molecular Biology Service Center - all located on the 4th floor of the Barbara Davis Center for Diabetes at the University of Colorado School of Medicine on the Anschutz Medical Campus in Aurora, Colorado.

NOTE: Website is constructed so that each link goes to the home page but opens a new window

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Autoantibody/HLA Islet Autoantibody Tests: Insulin Autoantibodies (mIAA); GAD65 Autoantibodies (GAA); IA-2 Autoantibodies (IA-2A); ZnT8 Autoantibodies (ZnT8A)

Other Autoantibody Tests: Transglutaminase Autoantibodies (TG); 21-Hydroxylase Autoantibodies (Hyd21); HLA Typing

Flow Cytometry Services: Cytometer training; real-time sample collection assistance and trouble shooting; technical consultation

Islet Services: Harvesting; animal protocol

ClinImmune Labs Description: ClinImmune Labs is a highly efficient academic business that integrates the highest quality laboratory services and products with its scholarly mission to contribute to the science of and education about stem cell therapy, histocompatibility and immunogenetics. Our company views a very important component of its business as consultative. Our staff provides education and technical advice to the research and medical community on stem cell processing and storage, histocompatibility testing, analysis and sequencing issues.

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Colorado Cord Blood Bank Description: The University of Colorado Cord Blood Bank (UCCBB), a major component of ClinImmune Labs, has a seventeen year history of collecting, processing, banking and distributing cord blood for human transplantation. UCCBB is a public cord blood bank, which has consented 30,000 women and banked over 8,500 cord blood units, of which more than 725 have been transplanted at over 150 different transplant centers in the United States and abroad. UCCBB is a major provider of Hispanic cord blood units to patients in the United States, Mexico, and Central and South America.

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Stem Cell Lab Description: ClinImmune Labs is accredited by the Foundation for Accreditation of Cellular Therapy (FACT) and provides hematopoietic stem cell processing for the University of Colorado Hospital Bone Marrow Transplantation Program. By combining the infrastructure for stem cell and cord blood processing, ClinImmune Labs achieves significant economies of scale that ensure cost-effective services for our clients.

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Flow Cytometry Description: The facility provides instrumentation, personnel, and expertise to assist users in multiparametric flow cytometry and fluorescence-activated cell sorting (FACS) for both research and clinical applications. The Flow Cytometry Facility follows Clinical Laboratory Improvement amendments (CLIA) guidelines and is certified by the College of American Pathologists (CAP).

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Histocompatability Description: Initial consultations provided for test selection, cost effectiveness, sample and turn-around time requirements; immunogenetics testing; transplant Immunology teleconferences; customized requisitions and clear, concise reports; electronic data transfer; additional test interpretation consults upon request; donor search strategy support; analysis of HLA allele frequency; HLA epitope analysis as part of population studies

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University of Colorado Cancer Center

Description: The University of Colorado Cancer Center offers cancer research facilities that are unparalleled in the Rocky Mountain region. Our Shared Resources provide a wide variety of specialized resource support to Cancer Center members to promote cancer research and enable the exploitation of cancer research opportunities.

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Biostatistics & Bioinformatics Description: The University of Colorado Cancer Center Biostatistics and Bioinformatics Shared Resource (BBSR) provides quantitative and information science support for the planning, design, analysis and presentation of basic science, clinical and epidemiological investigations by Cancer Center members

Services: • Consultation on study design • Consultation on sample size and power • Development of data collection, storage, and quality control procedures • Data analysis including genomic and proteomic data • Collaboration on manuscript and oral presentation preparation and grant proposal development

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Cancer Clinical Trials Office Purpose: The Cancer Clinical Trials Office (CCTO) provides support to clinical investigators on all activities related to the successful conduct of clinical trial research. This is the cornerstone to translate basic science knowledge of the cancer process into clinically useful treatment for patients.

Services: • Regulatory submission and monitoring • Protocol development • Budget development and contract negotiation • Enrollment of eligible patients • Data collection and monitoring • Specimen procurement and processing • Adverse event reporting • Coordination of patient treatment • Training and education of clinical and regulatory staff • Training of Phase I oncology fellows • General good clinical practice training • Maintenance of the CU Cancer Center Clinical Trials Database • Quality assurance; protocol review and monitoring • PQASC-audit schedule and data safety management plan

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Small Animal Imaging Service Purpose: The major goal of the small animal imaging program is establishment of novel imaging approaches to neuroscience, cancer, inflammation, developmental therapeutics using animal models and pharmacodynamic endpoints. State-of-the-art imaging facilities are extremely expensive and require advanced technical personnel.

Services: • MRI/CT/PET • Optical imaging • Metabolomics • Irradiation

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Flow Cytometry Description: Using flow cytometric analysis, high-speed cell sorting, and multiplexed fluorescent microsphere assays, the Flow Cytometry Shared Resource (FCSR) can perform a wide range of assays supporting cancer research, including cell cycle, cell proliferation, apoptosis, cell viability, cell signaling, stem cell detection, fluorescent protein analysis and cell phenotyping. These assays are crucial to understand how cancer derails normal cellular processes to cause disease.

FCSR has two sites, one at the University of Colorado Anschutz Medical Campus and one at National Jewish Health. The FCSR also serves as the Flow Cytometry Core for the Gates Stem Cell Center and the CU Skin Diseases Research Cores. We provide a consultation to help those CU Cancer Center members with little flow cytometry or immunology background to design appropriate experiments.

Services: • Flow cytometry analysis • Cell sorting • Luminex system • Vicell and cytospin • Education

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Functional Genomics Goals: The FGSR goal is to promote cancer-related research using Functional Genomics by: • Facilitating access to tools to investigate gene function at a genome-wide scale. • Providing protocols and expertise for the use of these genomic tools. • Promoting scientific exchange and discussion in the field of Functional Genomics.

Services: We provide lentiviral vectors encoding short hairpin RNAs (shRNAs) from the human and mouse TRC libraries. These libraries were originally created by the RNAi Consortium at the Broad Institute (MIT), and are now commercialized by SIGMA-Aldrich under the brand name MISSION® shRNA. • Glycerol bacterial stock • Plasmid DNA • Lentiviral suspension

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Genomics and Microarray Services: The Genomics and Microarray Shared Resource at University Of Colorado Denver Cancer Center is an advanced, state-of-the-art DNA and Protein microarray and Next Generation (NextGen) DNA sequencing technology center providing crucial research support for investigators interested in using:

• Next generation sequencing o Illumina HiSeq 2000/2500 sequencing o Illumina MiSeq sequencing o LifeTech IonPGM,sequencing

• DNA microarray o Illumina BeadArrays o Agilent Microarrays o Affymetrix GeneChips® Microarray or Plate Arrays

• Proteomics o Somalogic SomaScan

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Human Tumor Model-Based Databank

Description: The Human Tumor Model-based Databank Shared Resource assists University of Colorado Cancer Center investigators to establish their advanced disease specific models, and will develop a coordinated effort to create an –omics database that can be used for integrative analyses and queried remotely. Currently there are several-characterized disease-specific direct patient tumor models (DPTM), totaling close to 250 unique types. While it would be redundant to replicate this, a coordinated effort to give access to investigators to the multi-platform genomics data that is being generated is a unique opportunity.

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Molecular Pathology Description: The three complementary services in the Molecular Pathology Shared Resource provide fundamental technologies for CU Cancer Center researchers, including DNA sequencing, biospecimen evaluation and preparation, and cytogenetics analysis. Having expertise in these molecular research essentials greatly facilitates cancer research in Colorado.

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Cytogenetics Experience: We have experience with commercially available and laboratory developed probes, such as: • Chromosome enumeration probes • Painting probes • Probes for detection of chromosomal rearrangements • Single copy or locus-specific probes • Nick-translated DNA fragments

Services: • Single and dual color fluorescence in situ hybridization (FISH) assays • Multicolor-FISH assay • Unbanded chromosome analysis • Giemsa-trypsin (GTG) banded chromosome analysis

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Preanalytical Evaluation & Molecular

Testing Services

Description: The Pre-analytical Evaluation and Molecular Testing (PEMT) Service ensures the use of adequate and accurately classified tumor and ancillary specimens for molecular testing, and the application of specific predictive and prognostic molecular tests to appropriate tumor types for correlation with outcomes.

Services: • Retrieval of appropriate materials for testing (fluids, blocks or slides) from biospecimen archives • Review of histological slides to confirm diagnosis and ensure adequacy of specimen • Selection of appropriate technology for nucleic acid extraction (microdissection or coring of the block) • Preparation of sections for DNA sequencing (including for use with multiplex platforms [SNaPshot]

and whole exome isolation [Sureselect] and other molecular tests • Extraction of DNA from microdissected cells or tissue services • Testing of DNA by appropriate method • Entry of test results in database developed by the Research Informatics Shared Resource for

analysis and future correlation with outcomes • Tissue grossing, processing and embedding; cutting paraffin & frozen sections • H and E and special staining • Tissue array block creation • Immunohistochemistry, including antibody optimization

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Pharmacology Description and Mission: The CU Cancer Center Pharmacology Shared Resource (PSR), located at Flint Animal Cancer Center at Colorado State University in Fort Collins, Colo. was established to work with researchers and clinicians in a collaborative manner to design and implement studies to measure xenobiotics (drugs, toxicants, natural products, etc.) in biological systems and matrices.

• We are an analytical laboratory focused on drug measurement. • We help investigators with study design, sample collection, sample analysis and data

implementation. The mission of PSR is to assist in the prospective design of studies to assess drug exposure in biological systems, to measure drug levels using validated analytical assays, and to analyze, model and interpret the results.

Services: • Analyte quantitation • Compound or metabolite identification • Pharmacology study support • Pharmacokinetic and pharmacodynamic modeling; physiological based pharmacokinetic modeling • Population pharmacokinetic modeling • Metabolism studies • Drug transporter studies

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Protein Production/MoAB/Tissue Culture

Description: The Protein Production, Monoclonal Antibody, Tissue Culture Shared Resource (PPSR) supports basic and translational research at CU Cancer Center Members with validated cell lines, preparation of hybridomas, production of monoclonal antibodies, isolation and titering of baculovirus expressing recombinant proteins, and large scale preparation of recombinant proteins and monoclonal antibodies.

PPSR provides centralized services to produce cancer-related proteins for CU Cancer Center members to study their mechanism of action in identified drugs and cancer-related biomarkers.

Protein Services: • Mammalian system

o Phase I: Gene synthesis and subcloning o Phase II: Pilot protein expression o Phase III: Protein expression

• Baculovirus System o Phase I: Gene synthesis, subcloning, and baculovirus paration o Phase II: Protein expression and purification o Phase III: Larger scale protein expression and purification

Monoclonal Antibody Services: • Custom hybridoma services/monoclonal antibody production • Cell line repository/general cell culture services • Hyclone on-site FBS/media supply center

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Research Informatics Mission: • To provide secure, reliable, high-quality research databases and technology solutions that

facilitate cancer research for CU Cancer Center members. • To advance the field of cancer research informatics through local research and collaboration with

regional and national partners. We focus on solving specific problems for specific studies that can be implemented in an overarching research informatics infrastructure. This architecture extends the breadth of research possible at the CU Cancer Center and makes research as efficient as possible for all members.

Services: • Strategic informatics planning for individual grants and the CU Cancer Center as a whole • Analytics, design and selection or creation of new software systems • Database integration and data warehousing • Grid-based data sharing • Data extraction and report generation • Database quality assurance • Web site design and construction • Web/database server administration

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Tissue Biobanking & Processing

Description: The TBPSR provides personnel and expertise for biospecimen collection, processing and distribution; laser capture micro dissection (LCM); creation of tissue microarrays, and imaging and image analysis platforms.

The facility has more than 12,000 square feet of total workspace with 100 freezer bays and is fully equipped with an Arcturus XT LCM instrument with IF and UV capabilities, three Beecher Tissue Arrayers, an Aperio XT and two Syncroscopy tissue imaging systems and supporting computer systems.

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Biorepository Service Description: The TBPSR provides services for the collection, processing, annotation, storage, and distribution of biospecimens for research use.

Services: • Modular collection kits for biospecimen collection that allow for standardized collection and specimen

annotation from on and off-site locations • Body fluid processing and aliquoting • Surgical specimen procurement by specialized staff stationed in the pathology suite • Tissue fixation and embedding • Specimen archiving and inventory • Tissue microarray development and creation • Distribution of specimen and associated data for research projects • Provide remnant archival material from surgical pathology specimens for research projects • Imaging and image analysis (Aperio XT and Syncroscopy tissue imaging systems)

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Laser Capture Microdissection

Service

Description and Services: Laser Capture Microdissection (LCM) is a technique that allows scientists to lift specifically targeted cells from a section of tissue, leaving behind unwanted cells that could contaminate the molecular purity of subsequent analysis. This technology, which was developed in the late 1990s at NIH in collaboration with bioengineering and cancer pathology groups, is precise, accurate and semi-automated.

LCM uses a laser beam to activate a special transfer film. The film bonds to cells that have been identified and targeted by microscopy. From this film, macromolecules can be isolated and studied in downstream analyses.

An infrared (IR) laser is used for single cells and small populations of cells, while an ultraviolet (UV) laser can be used for large populations of cells. Starting tissue sections (5-7um) can be frozen or fixed/paraffin embedded, however it is ideal to use frozen sections for most downstream applications.

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Gates Center for Regenerative Medicine

Our comprehensive Center is open to all investigators within the University of Colorado system and beyond who are interested in stem cell research, including members affiliated with both the UC Anschutz Medical and Boulder campuses, Colorado State University, and the Colorado School of Mines.

Our Center is housed on the 8th floor of the Research Complex I on the Anschutz Medical Campus, the largest, new biomedical and clinical campus in the United States.

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Histology (Morphology and Phenotyping) Core

Objective: The objective of the Histology (Morphology and Phenotyping Core) is to provide investigators with the tools and expertise to process and analyze tissue samples. Although all tissue samples can be processed by the Core, the Core particularly specializes in skin samples.

Services: • Assistance with skin harvesting • Preparation of paraffin blocks • Sectioning of paraffin and frozen blocks • Standard H&E histology

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iPSC Core Services: • Isolation, amplification and cryo-preservation of of primary fibroblast from human or mouse skin

biopsy (for other cell types, please contact the Core) • Reprogramming of mouse and human primary cells into iPS cells (cells from other species can be

utilized as well, please inquire) • The iPS Core utilizes both lentiviral and sendai viral transduction methods which can be used to

routinely reprogram mouse embryonic fibroblasts (MEFs) or human primary fibroblast to a stem cell like state (iPSC) by over-expression of the four transcription factors Oct 4, Sox 2, Klf 4 and c-Myc (Yamanaka, 2006)

Validation and Characterization Services: • Embryoid body formation • Immunocytochemistry staining for pluripotency markers (Oct 4, Nanog, TRA-160, SSEA3, SSEA40) • Karyotyping • Mycoplasma testing

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Transgenics and Gene Targeting Core

Services: • Embryo cryopreservation and rederivation • Generation of ES cell lines from customer-provided mouse lines • Gene targeting in mouse ES cells • Mouse ES cell or iPS cell injections into mouse blastocysts • Pronuclear injections (conventional and BAC transgenes) • Zinc finger nuclease (ZFN)-mediated transgenesis • ZFN-mediated gene knockout in fertilized mouse eggs • Sperm and embryo cryo-preservation • In vitro fertilization

Laboratory Services: • DNA purification • Karyotyping and mycoplasma testing of stem cells

Miscellaneous Services: • Mating, weaning, tattooing and tail biopsies • Timed pregnancies

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Molecular Genetics Analysis Core

Services: • Consultation • DNA preparation and QC • Genotyping • NA sequencing • RNA expression analysis

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Gates Biomanufacturing Facility

Services: • Process development • Scale up • Manufacturing of both cell therapy- and protein-based products, to cGMP standards • Process documentation

Facilities: • Cell therapy manufacturing • Protein manufacturing; • Cryogenic and material storage • Utilities • Quality control lab • Cell development lab • Protein development lab • Quality assurance

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Structural Biology and Biochemistry Program Core Facilities

The following are shared facilities which are operated by the Program in Structural Biology and Biochemistry within UCD's School of Medicine Graduate School.

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Biophysics Description and Expertise: The Biophysics Shared Resource Facility is a user facility owned and operated by The Program in Structural Biology and Biochemistry at the University of Colorado. We opened our doors in early 2001 and have since served a wide variety of researchers on diverse projects in both academia and industry.

We currently have resources to study biomolecular structure and thermodynamics with surface plasmon resonance phenomonen, CD/ORD, ITC, DSC, fluorescence spectroscopy, and analytical ultracentrifugation (AUC). Mass spectroscopy, LC/MS/MS, HPLC, amino acid analysis are also available.

The Facility provides professional collaborations, training, and instrument time to researchers associated with both nonprofit organizations and private institutions. After a user is trained, they may use the instruments independently.

Instruments and Services: • Analytical ultracentifugation • Circular dichroism/optical rotary dispersion • Surface plasmon resonance • Differential scanning calorimetry • Isothermal titration calorimetry • Fluoresence spectroscopy • Amino acid analysis

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Computational Biology Description: The Computational Biology Shared Resource is not a facility in the physical sense. It is a service center with computer support provided by the Facility Manager, Mr. David Farrell.

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NMR Structural Biology Facility Description: The NMR Shared Resource Facility provides access to state of the art instrumentation to researchers at UCD and its affiliated institutions for the study of the structure and function of biologically important molecules implicated in the development and progression of diseases, including cancer, diabetes, aging, and infectious disease organisms.

Services: • Experimental design and training • Solution screening for molecular interactions • Solution analysis of small molecules • Structure analysis of proteins and nucleic acids

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Peptide and Protein Chemistry Facility

Description: Facility personnel help users choose and design experiments using appropriate chemistry/instrumentation, to obtain the necessary data, and interpret the results. The Facility also provides instruction for graduate students and postdoctoral fellows for the use of HPLC and LC/MS approaches.

Services: • Peptide synthesis • Purification and peptide/protein composition analysis • Mass spectrometry for academic and industrial clients

Instruments: • Beckman 6300 AA analyzer • Agilent 1100 HPLC systems • Agilent SL/XCT Electrospray MS systems • Advanced Chemtech peptide synthesizer

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Xray Crystallography Structural Biology Shared Resource at CU Cancer Center

Description: The X-ray crystallography facility was set up in 1999. The facility is fully equipped for biomolecular crystallization, crystal screening, data collection, data processing, structure-determination and model building. It currently has a a Rigaku Micromax 007 high frequency microfocus X-ray generator, a VariMax high flux optic with adjustable divergence, an AFC11 4-axis goniometer, a Pilatus 200K 2D area detector, and an Oxford Cobra cryo-system. HKL-3000R is used for data collection and processing and is run on a Linux CentOS Dell Precision Workstation.

Software: • hkl (denzo and scalepack): Data processing. Current version 1.96.6; • d*trek: Data processing. Current version DTREK72; • Chooch: A program for deriving anomalous scattering factors from X-ray fluorescence spectra; • CCP4: everything in crystallgoraphy except for data processing. Current version 4.1; • Epmr: A molecular replacement program • Replace: Another molecular replacement program • Shelx: Structural determination and refinement for small molecule. Also useful in finding heavy atom

positions as well as refinement of high resolution protein structures. Current version SHELX-97; • Solve and Resolve: MIR/MAD structure determination and solvent flattening. Current version 2.01; • CNS: structure determination and refinement. Current version 1.1; • O: model building. Current version 8.0; • Grasp: Graphical Representation and Analysis of Structural Properties; • Molscript and Bobscript: Graphical display of molecular 3D structures and electron densities; • Ribbons: Graphical representation of molecular 3D structures

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Biological Mass Spectrometry Facility

Overview: The goal of the UCD Biological Mass Spectrometry Facility is to provide investigators with the capabilities to identify, characterize and quantify biomolecules present in tissues, cells and biological fluids. The Facility houses both Proteomics and Metabolomics Cores, which aims to assist members with solving difficult or previously intractable problems in biomedical research. Methods for biomolecule isolation, separation, quantification, identification and bioinformatics analysis, together with expert guidance in study design, are integrated into expertise offered by the Facility. The Facility has access to diverse analytical technologies thereby allowing investigators to adopt multiple strategies and to independently verify their findings. The Facility also provides training in proteomic and metabolomic analysis and experimental design.

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Proteomic Services Services: • Protein identification from coomassie and silver stained gel bands • Protein identification from complex mixtures • Sample cleanup with C18 ZipTip or MW cut off filter • Protein quantitation: proteome wide (SILAC or label-free) and targeted analysis (MRM) • Post-translation modification analysis: phosphorylation, acetylation, methylation, and ubiquitination • Phosphopeptide affinity purification • Multidimensional protein and peptides fractionation • Depletion of IgG/albumin and high abundance proteins (albumin, IgG, IgA, IgM, antitrypsin,

transferrin, haptoglobin, fibrinogen, alpha2-macroglobulin, alpha1-acid glycoprotein, apolipoprotein AI & AII, complement C3, and transthyretin) from plasma/serum/CSF/mesenteric lymph samples to enrich low abundant proteins

• Intact molecular weight measurement

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Metabolomic Services Description: Metabolomic studies are performed on cells and media, biological fluids (blood, plasma, cerebrospinal fluid, etc.), tissues, organs, and tumor xenograft sections.

Services: • Single metabolite identification • General metabolomics screening • Relative quantification of metabolite levels • Targeted metabolic pathway analysis • Processing and validation of acquired data using metabolite databases and/or authentic standards • The core maintains a metabolite library of >650 authentic standards used for in-house matching of

retention times, MS fragmentation spectra, and relative quantification

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Skaggs School of Pharmacy Core Facilities

The Drug Discovery Initiative Core Facilities are housed at the Skaggs School of Pharmacy and Pharmaceutical Sciences on the University of Colorado Anschutz Medical Campus, offering specialized resources, services, and expertise to researchers across the USA and the world.

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Mass Spectrometry Description: The facility utilizes advanced mass spectrometry instrumentation and techniques to assist researchers with their proteomic and small molecule research.

Instrumentation: • Bruker Omniflex: A Bench top MALDI-TOF system suitable for both intact proteins and peptides • Waters Quattro LC: Triple quadrapole system suited for small molecule work and quanitation

experiments • Waters Q-Tof 2: Hybrid Quadrapole-Time of Flight instrument for intact proteins as well as small

molecule, exact mass work • Bruker HCT: Ion Trap equipped for nano-flow, high sensitivity proteomics experiments • ABI 4800 Plus: MALDI-TOF/TOF capable of acquiring high sensitivity, high mass accuracy data in

very short time frames. It has the additional advantage over standard MALDI-TOF of being able to perform MS/MS

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Medicinal Chemistry Description: The Medicinal Chemistry Core Facility can provide a range of services related to chemical services or drug lead development. The Medicinal Chemistry Core Facility is part of the Colorado Clinical and Translational Sciences Institute, CCTSI, an integrated and interdisciplinary statewide academic home for clinical and translational sciences.

Services: • Chemical synthesis • Assistance in lead compound identification and optimization • Structure-activity relationship (SAR) analysis • Improvement of chemical properties associated with ADME and/or toxicity issues • Literature searching

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High Throughput and High Content Screening Core Facility

Description: The High Throughput and High Content Screening (HTS/HCS) Core Facility specializes in chemical biology as it relates to drug discovery, biological probe discovery, and phenotypic characterization of small-molecule effects on cellular systems. In addition to housing the instrumentation and robotics required for traditional HTS, the Core houses a cutting-edge High Content image-based system for next-generation small-molecule discovery research and analysis in fixed or live cells and cellular systems.

The HTS/HCS Core is designed as a resource for the screening of small-molecule compound libraries in any cellular or biochemical assay system.

Automation Instruments: • 3-arm Janus Liquid Handling Robot • BioTek ELx405 Plate Washer • BioTek MultiFlo Bulk Liquid Dispenser HTS Assay Technology • EnVision Plate Reader HCS Assay Technology • Operetta High-Content Imaging and Analysis System

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Computational Chemistry and Biology Core Facility

Description: The Computational Chemistry and Biology (CCB) Core Facility provides a wide array of advanced tools and services for computational-based simulations and modeling of chemical and biological systems.

Services: • Equipment and software • Macromolecule modeling and analysis • Molecular simulations and quantum molecular mechanics • X-ray crystallography • Rational small molecule drug design, receptor-ligand interactions, and pharmacophore modeling • Predictive drug absorption • Molecular visualization

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ACCORDS: Adult and Child Center for Health Outcomes Research and Delivery Service

Description: ACCORDS replaces the SOM Center previously entitled “COHO.” The new name highlights the focus on the entire life spectrum as well as on “delivery science,” encompassing comparative effectiveness, patient-centered outcomes and implementation and dissemination research, which is in line with key funding sources. The Center encompasses both a Child Program (previously called “COR”) as well as an Adult Program which are fully integrated to share key administrative and research resources, thereby achieving economies of scale. Infrastructure support is provided jointly from the Children’s Hospital Colorado (CHC) and the Dean’s Office, SOM.

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Qualitative Core Objectives: The Core provides mentorship, training, and research assistance to faculty, investigators, and trainees interested in qualitative research. Core-affiliated faculty are available for mentorship and consultation on qualitative research methods, theory, and application.

Key Activities: The Core provides regular training on a variety of qualitative methods to interested members of the university community and community partners. These methods include, but are not limited to, in-depth interviews, key informant interviews, focus groups, ethnography, and grounded theory. The Core provides resources, including research staff trained in qualitative data collection and analysis, to faculty and investigators whose work involves qualitative components. The Core also supports the Qualitative Research Methods Forum (QRMF), an inter-institutional forum for qualitative health researchers in Colorado.

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Research Training Program Objectives: • To support and expand research training fellowship opportunities in T3-T4 translational research.

This encompasses the following research areas: health services, outcomes, comparative effectiveness, pragmatic trials and patient-centered research

• To provide work-in-progress groups for selected junior faculty to support their early development and progression to funding

• To provide consultation to Divisions or Departments who wish to develop a strong focus in outcomes or health services research

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Dissemination and Implementation Program (CRISP)

Program Objectives: • To lead as well as collaborate with others on implementation science and pragmatic research grants

and publications, at both local and national levels • To train researchers and practitioners in the above areas using various approaches, including on-line

resources • To assist applied researchers and practitioners in the development of interventions, study designs,

and evaluation plans to address priority areas for population impact • To identify, develop and provide practical measures and assessment approaches to support and

advance the above activities

Key Activities: • Provide trainings and presentations on implementation science topics in collaboration with the

ACCORDS Education Program, the CCTSI, and the VA IHD QUERI program • Collaborate and consult with other organizations (e.g., VA, Denver Health, KP) on grants, with initial

focus on local partnerships • Build upon and expand the CRISP website and create additional on-line resources and tools.

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Education Program Mission: The mission of the educational program is to foster an active learning community on state-of-the-art outcomes and community translational research methods and application.

Our educational focus is on methods in health outcomes research, health services research, dissemination and implementation (D/I) science, comparative effectiveness and patient-centered outcomes research, and delivery science.

Objectives: • Develop and provide high-value face-to-face educational workshops and on-line learning resources • Provide a monthly forum for discussion and dialogue on Insights and innovation in improving health,

continuing a dialogue with our ever-growing network of senior and junior faculty and staff • Increase funding potential (end-goal) • Establish a national educational presence and stay up-to-date on state-of-the-art methods at the

national level • Expand the reach of ACCORDS • Participate in national research agenda-setting conferences, committees and review panels

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Community Engagement and Outreach

Description: The Community Engagement core utilizes an innovative community-academic partnership structured and designed to optimize and mobilize our individual and collective capacity to promote innovation, collaboration and facilitation of quality, community-relevant science. Objectives: • Provide administrative infrastructure and formal opportunity for community-academic collaborations • Enhance public trust in the research enterprise • Target efforts to reduce health disparities • Increase investments in community based and translational research • Educate academic researchers and community members about the principles and methodology of

community engaged research

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Practice-Based Research Network (PBRN)

Description: Colorado Public Health PBRN (COPHPBRN) is a network of Colorado’s local public health agencies, state health department and researchers at the Colorado School of Public Health and the University of Colorado School of Public Affairs with a focus on Public Health Services and Systems Research. The network is housed at the Colorado Association of Local Public Health Officials (CALPHO) and co-directed with faculty from the Colorado School of Public Health.

Key Activities: PBRNs are ideally suited for studies that require engagement of primary care medical practices such as pediatric, family and internal medicine practices. These studies are in partnership with our member practices and seek to serve goals for not only completing the research, but also providing evidence on how to support primary care practice delivery and leave the practice with improvement strategies and new information. Studies focused on recruiting patients for a specific treatment or test are often not the most appropriate use of a PBRN.

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Health Informatics and m-Health

Objectives:To provide support and service to investigators regarding essential concepts in health informatics that are critical to their research and to the acquisition of funding. The core provides knowledge and expertise in the areas of data integration and combining disparate data sources, data harmonization, data modeling, database management, use of electronic health record data, clinical decision support, mobile health, data governance, and privacy and security.

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Biostatistics and Analysis Description: The Biostatistics and Analysis Core is made up of both Doctoral and Master’s level biostatisticians, epidemiologists, and data analysts. Areas of expertise include pragmatic trial design (cluster randomized designs, stepped wedge design, and multilevel modeling), as well as missing data in longitudinal studies, repeated events models, database design, and SAS programming.

Key Activities: • Provide study design, methodology and data analysis support • Collaborative research with health outcomes researchers • Instruction in quantitative methods in implementation research • Develop and publish original research in health services, and/or biostatistical methodology

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The Evaluation Center Services: The Evaluation Center offers a full range of evaluation services to our clients, including designing and conducting evaluations, integrating qualitative and quantitative analyses, and disseminating findings in a useful and meaningful way. We will work with you to develop a comprehensive plan that meets your unique needs, which will help you learn about and improve your program, as well as providing a rigorous and objective analysis of your program’s progress and effectiveness. • Design evaluation plans • Assess implementation and monitor progress • Measure outcomes and impact • Consulting services • Communicate findings

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Center for NeuroScience (CNS) and Rocky Mountain Neurological Disorders (RMND)

CNS Description: Members of the Center for NeuroScience may be students, postdoctoral fellows, residents or faculty of the University of Colorado, as well as the hospital affiliates of the SOM; including the University of Colorado Hospital, The Children’s Hospital, Denver Health Medical Center, the Denver VA Medical Center and National Jewish Hospital, with an interest in neuroscience. Individual Members must be actively involved in the conduct of clinical, research, or educational activities integral to the mission of the center.

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Microscopy Core (Core A) - RMND

Advanced Light Microscopy Core

Facility

Description: The Advanced Light Microscopy Core (ALMC), houses a variety of microscopes including a Zeiss 510 confocal microscope that will be equipped with a metadetector for simultaneous detection of multiple emission wavelengths with funds provided by our NINDS grant. The facility also includes a deconvolution microscope, a total internal reflection (TIRF) microscope and a rotating disk confocal.

Mission: The mission of the Advanced Light Microscopy Core is to facilitate labs from School of Medicine at University of Colorado Denver with access to advanced imaging research instrumentation at a reasonable price.

The core is currently housed within two buildings on the Anschutz Medical Campus: on 7th floor in Research 1 North and on 7th floor in Research 2. Training and support is provided for all ALMC instruments at no charge.

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Electron Microscopy Center

Description: The Electron Microscopy Center in the University of Colorado School of Medicine is a multi-user/service facility, administered by the Department of Cell and Developmental Biology. The mission of the EM Center is to provide biological-biomedical researchers with state-of-the-art instrumentation; conventional and energy-filtering transmission electron microscopy; and image analysis on a recharge basis.

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Targeting Vector Core (Core B) - RMND

Description: The core specializes in generating gene targeting and transgenic mouse models. The core has been serving the University of Colorado community since 2005. In that time, we have made over forty vectors including knock-in, knock-out, conditional knock-out, traditional transgenic and BAC transgenic. We have also developed specialized vector models for knock-in at the ROSA26 locus. For gene targeting models, our success rate for correct ES cell targeting is 95% and for germline transmission is 100%. The core also offers additional support services such as ES cell screening, BAC purification and mouse genotyping.

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Zebrafish Transgenic Core (Core C) - RMND

Description: The NINDS P30 Center Zebrafish Core has three Specific Aims: • Create transgenic lines of zebrafish that express different fluorescent proteins in specific populations

of pre- and postsynaptic neurons • Create transgenic zebrafish strains that express genetically-encoded calcium indicator dyes in

specific neuronal populations • Maintain transgenic and wild type zebrafish strains for the UCD neuroscience community

During the first year of the grant, the fish facility at the Fitzsimons campus is being optimized and we are focusing on Aim 3. In subsequent years, Aims 1 and 2 will be a priority. The Core can cover the expenses associated with breeding wild type fish for pilot experiments.

NOTE: Link point to tab for Core B so you can click the link for Core C

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Machine Shop (Core D) - RMND

Equipment: 1 manual milling machine with digital readout; 1 CNC milling machine (full 3-axis automation); 2 Lathes (one high-precision); 3 wood/metal-cutting band saws; 1 table saw; 1 precision surface grinder; 1 drill press; 1 12" disk/6" belt sander; 1 52" shear; extensive tooling & precision measuring devices

The shop routinely stocks a modest amount of material (acrylic, aluminum, brass, hardware) for small projects. Shop labor is provided at no cost to members of the Neuroscience Program.The shop labor rate is $46.30/hour for members of the Neuroscience Program and $89.78 for all others. Charges are based upon project time and material costs. Payment for services is made with a current university speedtype number.

NOTE: Link point to tab for Core B so you can click the link for Core D

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Animal Behavior Core - CNS Description and Services: The Animal Behavior Core provides investigators with access to high-quality behavioral testing equipment for mice and rats as well as consultation in all steps (from planning to publishing) of behavioral studies. In addition, the Animal Behavior Core provides: • Experimental strategy development: test selection, protocol design, and power analysis for proper

selection of animal numbers. • Behavioral testing: on-site training, pilot testing, and coordination with the animal facility. • Behavioral data analysis: tracking software, statistical analysis, presentation of results. • New task development: the Animal Behavior Core staff is available to help develop or institute other

behavioral tasks tailored to meet the needs of a specific project.

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Real Time QPCR Core - CNS Description: The core provides real time quantitative PCR services, including gene expression and copy number variation (CNV) analysis. Gene expression analysis is done using the relative standard curve method and an appropriate endogenous control.

• Gene Expression: The listed price is for initial consultation with client, reverse transcription and gene expression of your samples using real time PCR and data analysis and review. he assay (primer/probe) for your gene of interest is NOT included. This must be ordered separately by the client. All samples are run in triplicates.

The plate set-up price includes the set up of a 96-well plate, with all necessary controls. To the plate det-up price add the price per sample based on how many samples you will be running. A maximum of 8 samples can be run on a 96-well plate. Another plate set-up fee will be charged for each additional 96-well plate needed to accommodate your number of samples.

• CNV: The plate set-up price is for initial consultation with client, analysis of your samples and data review. The assay (primer/probe) for your gene of interest is NOT included. This must be ordered separately by the client. To the plate set-up price add the price per sample based on how many samples you will be running. A maximum of 11 samples can be run on a 96-well plate. Another plate set-up fee will be charged for each additional 96-well plate needed to accommodate your number of samples. All samples are run in triplicates.

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Optogenetics Core - CNS Description and Services: The Optogenetics Pilot Program falls under the Department of Physiology and Biophysics. The goal is to facilitate the incorporation of optogenetics methods into existing research programs at the Anschutz Medical Campus. Optogenetics encompasses a set of methods by which cellular function can be manipulated with light, allowing unprecedented spatiotemporal control of a rapidly expanding set of biological activities. We hope to make this set of tools more accessible to novice users on campus via the following services: • Design and fabrication of light sources and delivery devices for in vivo/vitro optical stimulation. • Consultation on adopting/optimizing the use of optogenetic methods • Construction and maintenance of a confocal microscope for ex vivo and in vitro optogenetics and

electrophysiology experiments • Design and production of custom hardware components • Supply equipment to assist optogenetic experiments

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CardioVascular BioImaging Core Goals: Dedicated research facility for the acquisition and analysis of state of the science ultrasound imaging of the heart and vasculature. The Core allows researchers the ability to comprehensively assess vascular and cardiac health using the most advanced ultrasound and echo technology available. Investigators will also benefit from a centralized imaging processing center.

Partners:The Colorado Clinical Translational Science Institute (CCTSI), Center for Women’s Health Research (CWHR), Vascular Biology Initiative (VBI), and the Division of Cardiology and Department of Bioengineering.

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Vascular Imaging Imaging Techniques: • Endothelial function using flow-mediated dilation (FMD) of conduit arteries including the brachial,

femoral and radial arteries. • Arterial stiffness/compliance of central and peripheral arteries including the carotid artery. • Carotid intimal medial thickness (c-IMT), a surrogate measure of atherosclerosis • Quantitative assessment of smooth muscle cell function using dilation response to nitroglycerine. • Arterial blood flow of peripheral arteries including carotid, femoral, and brachial.

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Cardiac Imaging Description: Latest echo technology, including real-time three-dimensional (3D) and 4D, and speckle tracking imaging. Real-time 3D and speckle tracking echocardiography are new technologies that give accurate measures of regional and global cardiac function. These technologies rival information obtained by more expensive modalities (like cardiac MRI) and have been used to detect subtle changes in both ventricles and atria in many disease processes. Together these ultrasound-based modalities provide non-invasive and cost-effective measures of CV health which can detect preclinical dysfunction, inform prognosis, assess risk, track responses to CV therapy, and assess CV side effects of non-CV therapies. Additionally, when combined with vascular assessments, these tools provide researchers with a complete evaluation of cardiac-vascular coupling that is affected by many disease states.

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Technique Development Assistance in: • Appropriate research design including cardiovascular imaging outcomes to be assessed, sample size

determination, etc. • Development of new imaging approaches and techniques. Specific targets include:

o impedance, to quantify total vascular load o local stiffness, to quantify local mechanical changes and remodeling o wall shear stress, to link vascular mechanics to mechanistic signaling o ventricular-vascular coupling, to measure heart reserve function o 3D strain, to measure regional myocardial contractility in all 3 directions with single beat analysis

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Educational Services Description: Faculty members are available to interact with junior faculty members who utilize cardiovascular imaging techniques in their studies for necessary mentorships and collaborations to ensure the research success of trainees and junior faculty. Additionally, the Core offers the Core facility as a resource available to TL1, KL2, BIRWCH and individual K scholars, where they can learn to perform cardiovascular imaging techniques important for clinical research in cardiovascular health. Interested student/fellows and junior faculty will have the opportunity to work under the guidance of Drs. Moreau and Dorosz and the trained sonographers. They will receive formal instruction in appropriate study design and technical aspects to imaging and data analysis so that they can become proficient in cardiovascular image acquisition and/or analysis offered through the Core. In addition to technical skill training, the core will integrate other formal didactics and educational opportunities at UCD to assist with student/fellow training and junior faculty career development.

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Colorado Genetics Laboratory Description: Colorado Genetics Laboratory (CGL) is the premiere lab for cytogenetics and cytogenomics in the Rocky Mountain region. We are nationally recognized for excellence and provide state-of-the-art cytogenetic testing, as well as full interpretation of results and excellent customer service. Our Laboratory Directors and Genetic Counselors are available to discuss appropriate testing and test interpretation. In-service presentations and clinical conference support are also available.

Based in Denver, CGL offers full service laboratory testing in prenatal, postnatal, cancer cytogenetics, fluorescence in situ hybridization (FISH), and chromosomal microarray (CMA).

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Prenatal Testing Tests: • Amniotic fluid chromosome analysis • Chorionic villi sample (CVS) chromosome analysis • Percutaneous umbilical blood sample (PUBS) chromosome analysis • Fluorescence in situ hybridization (FISH) • Culture for biochemical or molecular testing • Culture and freeze • Alpha-fetoprotein (AFP) and acetylcholinesterase (AchE)

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Postnatal Testing Tests: • Chromosomal microarray • Blood chromosome analysis • High resolution chromosome analysis • FISH for microdeletion/microduplication syndromes • Tissue testing

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Cancer Testing Tests: • Bone marrow chromosome analysis • Blood chromosome analysis for acquired abnormalities • Solid tumor or lymph node chromosome analysis • FISH for chromosome abnormalities in cancer • FISH for chimerism studies

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Tissue Testing Tests: • Pregnancy loss microarray • FISH for chromosome abnormalities common in pregnancy loss • Tissue chromosome analysis • Culture for molecular or biochemical testing • Culture and freeze

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Fluorescence in situ Hybridization (FISH)

Tests: • Trisomy screen by FISH for detection of trisomy 21, 18 and 13, and enumeration of sex

chromosomes • FISH for microdeletion/microduplication syndromes • FISH for gender determination • FISH for chromosome abnormalities common in pregnancy loss • FISH for chromosome abnormalities in cancer • FISH for chimerism studies in bone marrow transplant patients

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Chromosomal Microarray Tests: • Pediatric and Adult (Postnatal) CytoSNP-850K Microarray • Pediatric and Adult (Postnatal) CytoSNP-850K Microarray plus 5 cell chromosome analysis • Pregnancy Loss Microarray • Prenatal Microarray (coming soon)

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Denver Genetic Laboratories DNA tests: • Gene mutation analysis and DNA panels Biochemical tests: • Metabolite tests on urine, serum, plasma, and cerebrospinal fluid • Enzyme activity assays on fibroblasts and leukocytes Molecular genetics tests: • Mitochondrial enzyme activity assays and blue native page on fibroblasts and biopsy tissues

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Department of Pathology Description: The Department of Pathology at CU is a “hybrid” basic science and clinical department based at the Anschutz Medical Campus in Aurora, a northeastern suburb of Denver, Colorado. The Department seeks to establish more active partnerships through the community, our region and our profession as we grow to provide value based medicine, focused on the origins of human disease.

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Autopsy Service is located at University of Colorado Hospital Home

Colorado Molecular Correlates Lab (CMOCO)

Description: state-of-the-art facility, located within the University of Colorado Department of Pathology. The CMOCO Laboratory is dedicated to the development and implementation of predictive, prognostic and diagnostic molecular biomarker testing that will permit selection of cancer treatments with targeted therapy allowing for the best in personalized medicine.

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Cytology Description: The Cytopathology Laboratory at the University of Colorado is a full-service laboratory accredited by both the College of American Pathologists (CAP) and the Joint Commission (JC). We provide routine screening and diagnostic testing of gynecological, non-gynecological, and fine needle aspiration specimens.

It is our mission to provide our clients with the highest possible quality service using continuous quality control and internal personnel training procedures.

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Clinical Lab Service is located at University of Colorado Hospital Home

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Histology Description: The histology / immunohistochemistry laboratory, located within the University of Colorado Department of Pathology, is a state-of-the-art facility ready to serve all of your tissue processing needs. With an experienced staff, up-to-date equipment, and a wide menu of tests available, we can offer timely and comprehensive services for clients of all sizes.

Services: • Full services for frozen tissues • Immunofluorescence • In situ hybridization • Immunohistochemical staining

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Prostate Diagnostic Lab Description: The Prostate Diagnostic Lab serves as the central pathology site for many large clinical studies sponsored by the NIDDK, SWOG, and private industry. We also serve as the central core lab for Biology of the PCPT Program and the MAPP Research Network.

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Surgical Pathology Service is located at University of Colorado Hospital Home

Altitude Research Center (ARC) Mission: Improving life through research on how hypoxia affects health and performance

Goal: Developing distinguished research programs in four key areas: • Epidemiology of altitude problems in travelers and the impact of hypoxia on common diseases in

residents of altitude • Clinical outcomes research • Integrative physiology • Cell, molecular and genomic mechanisms of hypoxia

Programs: • Epidemiology • Clinical outcomes research • Integrative physiology • Cellular, molecular, and genetic biology

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Systems Biology Core Facility Description: The Systems Biology Core Facility provides strategic consulting and integrated technologies for analysis of a wide range of biomolecules. We offer collaborations and core services for the University of Colorado Denver community and researchers worldwide in

• proteomics • metabolomics • nucleic acid analysis

Our technologies include nanoscale liquid chromatography tandem mass spectrometry (LC-MS/MS) on quadrupole and hybrid linear ion traps; multiplexed, microscale ELISA-based protein arrays; quantitative real-time PCR and redox separation

Services: • Shotgun proteomics • Isoelectric focusing • Two-dimensional gel separation • Targeted proteomics • Multiplexed microscale ELISA • Preparative-scale HPLC • One-dimensional gel separation • Metabolic analysis • Glycan analysis • Quantitative PCR

Instrumentation: • Quadrupole ion trap mass spectrometer • Hybrid triple quadrupole/linear ion trap mass spectrometer • Digital proteome fractionator for highly reproducible isoelectric focusing • MesoScale discovery system • Coularray • Equipment for multiplexed one and two-dimensional gel electrophoresis

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Center for Advancing Professional Excellence (CAPE)

Vision: Our vision is to develop the safest, most effective, most compassionate and highest quality health care professionals and teams through education, assessment, innovation and research.

Mission: Educate; advance scholarship and research; impact patient care; reach out to and support community

Services: Standardized patient program; teaching assistant program; simulation; remediation and test preparation; faculty and fellow development; digital production and filming

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Center for Surgical Innovation Description: The Center for Surgical Innovation (CSI) at the University of Colorado is a multidisciplinary surgical training center dedicated to promoting educational courses and development of leading edge surgical techniques and technologies. We are affiliated with the Department of Surgery in the CU School of Medicine.

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Colorado Translational Research Imaging Center (C-TRIC) in the Department of Radiology

Description: C-TRIC is a comprehensive research imaging center on the University of Colorado Anschutz Medical Campus (CU-AMC). Established in 2010 from SIRC funds and the support of the CU departments, the goal of C-TRIC is to create for the campus a collaborative, research imaging environment by bringing together researchers from different disciplines with imaging scientists, and providing the organizational structure and a state-of-the art imaging facility that maximizes creative translational discovery.

Services: • Human imaging • Animal imaging • Optical imaging • Quantitative image analysis • Imaging informatics • Imaging education

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Environmental Health and Safety Description: Environmental Health and Safety (EHS) supports the University's policy to minimize the impact on the general population as a result of its activities through the development, tracking and implementation of several management programs and services. Some of the programs and services that are available through EHS are training, auditing, emergency response, biological, chemical and radioactive waste management as well as occupational health monitoring services.

Primary Areas of Service: • Biosafety • Chemical materials and waste management • Compliance assistance • Green tagging program • Occupational health • Radiation safety • Safety • Shipping and receiving hazardous materials • Training

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Animal Care and Use Program The Office of Laboratory Animal Resources believes that the purpose of animal research is to contribute to the enhancement of human and animal health. Animal use is a privilege that requires close adherence to a clear and defined code of ethics to ensure all animals are treated as humanely and judiciously as possible. All research and teaching projects are required to consider alternatives to animal use and to utilize those alternatives when they can result in sound scientific conclusions.

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Animal Care and Use Program (IACUC)

Description: The Institutional Animal Care and Use Committee (IACUC) is responsible for the oversight and evaluation of the Institution's animal care and use program, procedures and facilities, ensuring compliance with the recommendations in the Guide for the Care and Use of Laboratory Animals, Animal Welfare Act and PHS Policy.

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Office of Laboratory Animal Resources (OLAR)

OLAR Description: The Mission of the Office of Laboratory Animal Resources (OLAR) is the advancement of innovative medical research by utilizing leading edge technology and a compassionate/knowledgeable staff who provide quality care for and enhance the lives of animal research models.

OLAR Facilities: The Center for Comparative Medicine has two surgical suites and can accommodate up to six large animal surgeries at one time. Our skilled veterinary technician staff has experience with induction, anesthetic maintenance, recovery, and post-operative care of a wide variety of species including dogs, cats, rabbits, pigs, rats, mice, ground squirrels, and chinchillas. Our knowledgeable staff can also train people to perform a variety of sterile surgical techniques and procedures.

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Colorado Biostatistics Consortium (CBC) in the Colorado School of Public Health

Description: The CBC resides in the Department of Biostatistics and Informatics (DBI) in the Colorado School of Public Health. Our goal is to facilitate the Department's collaborative mission. The CBC along with the DBI strive to maintain adequate capacity and expertise for biostatistics consultation and to seed collaboration development with researchers at the University of Colorado Anschutz Medical Campus, its affiliates, and the surrounding community.

Available Resources: • Grant preparation • Study design • Sample size • REDCap • Data analysis • Education

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Colorado Clinical and Translational Sciences Institute (CCTSI)

Description: CCTSI supports translational research by providing resources, integrating facilities and services, developing innovative research tools, and catalyzing interdisciplinary research programs. Must join the CCTSI to use the services and resources - membership is free.

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Network of Translational Technologies (NeTT)

NOTE: The following resources are described and linked elsewhere in this document under the School, Department, or Center that they are most closely affiliated with. However, CCTSI can provide investigators access to these facilities at subsidized rates: • Advanced Light Microscopy Core Facility (ALMC) • Colorado Translational Research Imaging Center (C-TRIC) • Gene-Targeted Vector Generation • Genomics and Microarray Core • Transgenic and Gene Targeting Core • Metabolomics • Proteomics Consortium • Pharmacology Core • Medicinal Chemistry Core • Biobank Consortium

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Biostatistics, Epidemiology, & Research Design (BERD)

Description: We provide statistical collaboration and resources to CCTSI investigators and trainees. The BERD is funded collaboratively by CCTSI, Colorado Biostatistics Consortium (CBC), and the Children's Research Institute (RI). We facilitate clinical and translational research through:

• Statistical consultation and collaboration • Improved statistical literacy through seminars and short courses • Providing access to advanced statistical tools

CCTSI subsidizes the cost of approximately 40 hours of work for project development, small projects, and for support of CCTSI training/pilot grant awardees. Through support from School of Medicine, we are also able to provide some free collaboration through the BERD SEED program.

Biostatistics Training and Education: • Biostatistics Short Course • Biostats 101 Seminar Series – archived videos • Biostatistics for Non-Statisticians Seminar Series – archived videos • How much Biostatistics/Epidemiology training do you need?

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Child & Maternal Health Description: The overall goal of the CMH Program is to support and promote clinical and translational research in children of all ages, pregnant women, and the mother-child dyad to improve child health and prevent diseases, thus preempting adverse outcomes that increase disease burden and the cost of health care over the life course.

Resources, Funding, and Training Opportunities: • Children's Hospital Colorado Clinical and Translational Research Center (CTRC) • Perinatal Clinical and Translational Research Center (CTRC) • Child and Maternal Health Pilot Grant Program • Children's Hospital Colorado Research Institute • TL1 Pre-Doctoral Training Award • KL2 Research Scholar Award

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Clinical & Translational Research Centers (CTRC)

Description: Provides infrastructure, resources, and dedicated space to conduct clinical research. Available resources include research nursing, nutrition, informatics, statistics, regulatory and compliance support, and core laboratory services. All services are provided as fee-for-service resources. Only IRB-approved protocols can request CTRC services.

Resources: • Blood draws including those for pharmacokinetic (PK) studies • Whole room calorimetry and dedicated facilities for sleep studies • Infusion and medication administration • Measurement of insulin sensitivity via OGTT, IVGTT, MTT, and hyperinsulinemic-euglycemic clamp • Nursing and advanced practice assistance for research procedures such as biopsies and

bronchoscopies • Body composition measurement via DEXA, BOD POD, and PEA POD • Collection of fetal and maternal biological samples including cord blood • Lab tests for metabolites, hormones, cytokines, flow cytometry, etc. • Cardiovascular imaging (Echo services) • Provision of standardized meals for intervention and energy balance studies • Measurement of dietary intake and energy expenditure • Access to statistical support • Access to software for data management and confidential questionnaires administration • Assistance with ethical protocol design and research participant advocacy

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Colorado Profiles Description: The “Colorado PROFILES” search engine for biomedical research expertise and networking provides tools to help investigators and students find experts, potential collaborators or mentors, and to view past and present research networking of our investigators.

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Pragmatic Trials & Dissemination/Implementation Research

Description: Catalyzing and supporting the development of pragmatic trials, comparative effectiveness research methods, and implementation science to achieve increased efficiency and economies of scale. We aim to foster a learning community and serve as a conduit for active dissemination of resources from NIH and other national organizations.

We are partnered with the Adult and Child Center for Health Outcomes Research and Delivery Science (ACCORDS), funded by the University of Colorado School of Medicine and Children’s Hospital Colorado.

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Regulatory Knowledge & Support

Description: The Regulatory Knowledge and Support Core (RKSC) of the CCTSI provides a support structure to deal with the complexities of research regulatory compliance.

Services and Resources • Clinical research support center • Regulatory training and education • Regulatory and compliance consultation services • Human research subject advocate (RSA) • Participant rights • Safety monitoring (DSMPs) • Regulatory resources • Tools and forms

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Research Ethics Consult Service

Description: The Research Ethics Consult Service is a free service sponsored by the CCTSI. It is available to all biomedical and behavioral researchers at the University of Colorado Denver, as well as its clinical affiliates, who seek advice about ethically complex aspects of their research.

Services: Examples of the kind of questions that the support service might assist you with include, but are not limited to, the following: • How do you know if the consent process that you are anticipating using for your research is the most

appropriate one, given special characteristics of my research population? • Should you do anything about the fact that your research is controversial in the eyes of many in the

public? • How should you determine how far to go in your efforts to minimize the risks of your research,

especially if it might compromise your ability to test your hypotheses? • How can you assure that your research team is honoring the privacy of our research participants,

given the access we have to confidential information?

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Research Studio Program Description: The Studio Program offers a dynamic and integrative way of increasing and improving the quality of clinical and translational research. A Studio session is a structured and collaborative roundtable discussion bringing together relevant research experts to help investigators with specific questions at a specific stage in the research process. The "Research and Development Studio" process is based on models from industry that demonstrate that multidisciplinary content experts can increase research impact.The service is free and funded by the CCTSI.

Consultation Types: • Hypothesis generation • Study design • Implementation • Grant review • Analysis and interpretation • Manuscript review • Translation

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Translational Informatics Description: Translational informatics applies information science to help turn research data into knowledge: it incorporates academic disciplines including artificial intelligence, cognitive science, computer science, information science, and social science. The general goal of informatics is to develop systems that assist users by making it easy to store, retrieve, and use data, information, and knowledge.

Services and Resources: • REDCap (Research Electronic Data Capture) • Bioinformatics tools and techniques:

o High-Throughput Genetic Analysis o Brief educational videos on a variety of bioinformatics topics o AMC bioinformatics journal club

• Data sharing • Data management plan tool

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Education, Training, & Career Development (ETCD)

Description: The Education, Training, and Career Development (ETCD) program provides clinical-translational scientists and trainees with knowledge, training, and career skills. ETCD offerings span critical periods, from the beginning of research training at the pre-doctoral level through senior faculty. The aim of the ETCD is to create a robust local workforce and a national leadership pool for clinical-translational research.

Opportunities: • Clinical science graduate program • Clinical faculty scholars program (CFSP) • KL2 (K12) research scholar awards • TL1 (T32) pre-doctoral fellowships • Clinical research training for investigators and research assistants • Colorado mentoring training (CO-Mentor) program • Denver student training in research science (STaRS) program • Leadership for innovative team science (LITeS) • K to R transition (KTR) program • ATLAS.ti short course • Summer undergraduate minority mentoring in translational science (SUMMiT) program • Pre K program

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Office of Grants and Contracts Description: The mission of Grants and Contracts is to provide service to principal investigators and administrators for the purpose of obtaining and administering extramural funds in compliance with University and sponsor guidelines.

Services: • Budget review • Grant application and contract proposal review • Contract review, negotiation, and acceptance; • Subrecipient agreement development, negotiation, and acceptance • Providing advice on sponsor and University requirements

Responsibilities: • Financial reporting and standard invoicing (e.g. does not include non-governmental clinical trial

invoicing which is done by academic units) • Providing advice on sponsor and University requirements • Acting as a liaison with sponsor contract/financial representatives • Cash management • Acting as a liaison for audits of sponsored activity

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Statistical Consulting Services in the College of Liberal Arts and Sciences

Description: We provide quantitative consulting of many kinds to the university community, to industry and government in the Denver metropolitan area, and to state, federal, and international agencies. For university graduate students engaged in thesis research this service is free, all others pay on a sliding scale. Our consultants range from university professors with thirty years of consulting experience down to PhD- and MS-level graduate students, who work on selected projects under faculty supervision.

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Health Sciences Library In addition to the usual library services (check-out, books, e-books, research materials, databases, journals, etc.), the library offers quite a few technical resources including:

• Borrow laptops and devices • Take classes • Use one of the 56 workstations • Ask a question online • Professional literature search • Reserve a group study room • Reserve a meeting room • Use the services at the Writing Center

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JFK Partners Description: JFK Partners is an interdepartmental program of the departments of Pediatrics and Psychiatry of the University of Colorado School of Medicine located at the Anschutz Medical Campus in Aurora, Colorado. Designated as Colorado's University Center of Excellence in Developmental Disabilities (UCEDD) and Leadership Education in Neurodevelopmental Disabilities (LEND) Program, JFK Partners has collaborative relationships with numerous organizations that are a part of Colorado's developmental disability and special health care needs communities.

Publications and Products: • A Guidebook for Child Welfare and Part C Agencies • Autism and Down Syndrome Fact Sheet • Care Coordination Resource Guide • Denver Developmental Screening Tool • ENRICH Early Intervention "Reunion Videos" • Facing Your Fears Facilitators Manual • Gone Through Any Changes Lately? (DVD) • Just Being Kids DVD and Facilitators Guide • Mental Health Consultation in Early Care and Education: A Resource and Sustainability Toolkit for

Providers • Navigation Guide: Early Childhood Mental Health • Promoting Physical Fitness in Children & Adolescents with Disabilities: A Parent's Guide &

Community Members • Resources for First Responders that Encourage Optimal Interactions with People with Developmental

Disabilities • Toilet Training for Children with Special Needs (DVD) • Online courses • Archived webinars

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