Compound MicroscopeCompound MicroscopeCompound MicroscopeCompound Microscope

How to properly carry the microscope

• Two-dimensional images • View internal structures

With a binocular microscope, adjust oculars for both eyes!

Stereoscopic (Dissecting) Microscope

• Three-dimensional images• View external structures

Stereoscopic close-up of finger

Microsurgery being performed with a stereoscope

Fluorescence MicroscopyFluorescence MicroscopyFluorescence MicroscopyFluorescence Microscopy

Cells are stained with Cells are stained with fluorescent dyes called fluorescent dyes called fluorochromes.fluorochromes.

Uses UV light as energy source.Uses UV light as energy source.

Fluorochromes absorb UV light Fluorochromes absorb UV light

and emit visible light.and emit visible light.

Produces green to yellow colorProduces green to yellow color

Digital Microscope SystemDigital Microscope System

Automated system: Auto-focus, auto-aperture, auto-light……

Electron MicroscopyElectron MicroscopyElectron MicroscopyElectron Microscopy

Uses a beam of electronsUses a beam of electrons Very high magnification of up to 100,000 X.Very high magnification of up to 100,000 X.

Transmission electron microscope (TEM):Transmission electron microscope (TEM):• Two-dimensional images Two-dimensional images

• View internal structuresView internal structures

Scanning electron microscope (SEM):Scanning electron microscope (SEM):• Three-dimensional imagesThree-dimensional images

• View external structuresView external structures

TEM and SEM ComparedTEM and SEM ComparedTEM and SEM ComparedTEM and SEM Compared

Scanning Electron Microscope Transmission Electron Microscope

Transmission Electron MicroscopyTransmission Electron MicroscopyTransmission Electron MicroscopyTransmission Electron Microscopy

(a )a virus and )b( a protozoan. E. coli

Scanning Electron MicroscopyScanning Electron MicroscopyScanning Electron MicroscopyScanning Electron Microscopy

Paramecium Aspergillus