Fluorescence MicroscopyFluorescence MicroscopyFluorescence MicroscopyFluorescence Microscopy
Cells are stained with Cells are stained with fluorescent dyes called fluorescent dyes called fluorochromes.fluorochromes.
Uses UV light as energy source.Uses UV light as energy source.
Fluorochromes absorb UV light Fluorochromes absorb UV light
and emit visible light.and emit visible light.
Produces green to yellow colorProduces green to yellow color
Digital Microscope SystemDigital Microscope System
Automated system: Auto-focus, auto-aperture, auto-light……
Electron MicroscopyElectron MicroscopyElectron MicroscopyElectron Microscopy
Uses a beam of electronsUses a beam of electrons Very high magnification of up to 100,000 X.Very high magnification of up to 100,000 X.
Transmission electron microscope (TEM):Transmission electron microscope (TEM):• Two-dimensional images Two-dimensional images
• View internal structuresView internal structures
Scanning electron microscope (SEM):Scanning electron microscope (SEM):• Three-dimensional imagesThree-dimensional images
• View external structuresView external structures
TEM and SEM ComparedTEM and SEM ComparedTEM and SEM ComparedTEM and SEM Compared
Scanning Electron Microscope Transmission Electron Microscope
Transmission Electron MicroscopyTransmission Electron MicroscopyTransmission Electron MicroscopyTransmission Electron Microscopy
(a )a virus and )b( a protozoan. E. coli
Scanning Electron MicroscopyScanning Electron MicroscopyScanning Electron MicroscopyScanning Electron Microscopy
Paramecium Aspergillus