Comparative analysis of aberration yields induced by radiation with different LET

(Linear Energy Transfer)

University of Warsaw Jakub Pietrzak UW

University of Gdańsk Magdalena Rojek Monika Szymanska

AGH University of Science and Technology Anna Kaminska Dominika Kedzierska Ewa Lagudza

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THE DREAM TEAM

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Laboratory of Radiation Biology

Marta Deperas- Kaminska (supervisor) Ekaterina Zaytseva

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The practice purposes:

Theoretical introduction to cytogenetic methods used in biodosimetry

Instructions of safety rules of work with human blood samples.

To learn how to irradiate human lymphocytes and culture them under experimental conditions.

Be able to carry out a simple experiment and analysis with irradiated cells

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SCHEME OF THE EXPERSCHEME OF THE EXPERIIMENTMENT

Incubation for the standard metaphase analysis

colcemid

47h

Fixation

Staining with Giemsa50h

Standard light

microscope

Radiation

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Our experiment

Picture 2 Lymphocyte http://en.wikipedia.org/wiki/Image:SEM_Lymphocyte.jpg

1. Irradiation of blood samples with different LET radiation

2. Lymphocytes culture (48 hours)

Picture 1 Bloodhttp://en.wikipedia.org/wiki/Lymphocyte

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3. Lymphocytes fixation

4. Giemza staining

5. Analysis of chromosomes aberrations

Picture 3 Chromosomeshttp://humogef.ifj.edu.pl/zaklad/foto13.jpg

Picture 4 Chromosomes 2http://humogef.ifj.edu.pl/zaklad/foto13.jpg

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Dic

Centric ring

Acentric ring

deletion

ace

Unstable aberrations - dicentrics, acentric fragments and centric and acentric rings in Giemsa

stained metaphase

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gamma protons C12 Li7 B11

LET (keV/um)

0 0.3 0.5 12 20 55

ab

err

ati

on

fre

qu

en

cy

0

100

200

300

400

sum of all kinds of aberration per 100 cellsnumber of dicentrics per 100 cells

The increase of aberration yields with increase of radiation LET in human peripheral blood lymphocytes is observed.

RESULTS:

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Acknowledgements

Special thanks to:

Dmitri Vladimirovich Fursaev

Wladyslaw Chmielowski, Roman Zawodny

Marta Deperas-Kaminska, Katia Zaytseva

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THANKS FOR YOUR ATTENTION