Comparative analysis of aberration yields induced by radiation with different LET
(Linear Energy Transfer)
University of Warsaw Jakub Pietrzak UW
University of Gdańsk Magdalena Rojek Monika Szymanska
AGH University of Science and Technology Anna Kaminska Dominika Kedzierska Ewa Lagudza
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The practice purposes:
Theoretical introduction to cytogenetic methods used in biodosimetry
Instructions of safety rules of work with human blood samples.
To learn how to irradiate human lymphocytes and culture them under experimental conditions.
Be able to carry out a simple experiment and analysis with irradiated cells
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SCHEME OF THE EXPERSCHEME OF THE EXPERIIMENTMENT
Incubation for the standard metaphase analysis
colcemid
47h
Fixation
Staining with Giemsa50h
Standard light
microscope
Radiation
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Our experiment
Picture 2 Lymphocyte http://en.wikipedia.org/wiki/Image:SEM_Lymphocyte.jpg
1. Irradiation of blood samples with different LET radiation
2. Lymphocytes culture (48 hours)
Picture 1 Bloodhttp://en.wikipedia.org/wiki/Lymphocyte
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3. Lymphocytes fixation
4. Giemza staining
5. Analysis of chromosomes aberrations
Picture 3 Chromosomeshttp://humogef.ifj.edu.pl/zaklad/foto13.jpg
Picture 4 Chromosomes 2http://humogef.ifj.edu.pl/zaklad/foto13.jpg
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Dic
Centric ring
Acentric ring
deletion
ace
Unstable aberrations - dicentrics, acentric fragments and centric and acentric rings in Giemsa
stained metaphase
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gamma protons C12 Li7 B11
LET (keV/um)
0 0.3 0.5 12 20 55
ab
err
ati
on
fre
qu
en
cy
0
100
200
300
400
sum of all kinds of aberration per 100 cellsnumber of dicentrics per 100 cells
The increase of aberration yields with increase of radiation LET in human peripheral blood lymphocytes is observed.
RESULTS:
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Acknowledgements
Special thanks to:
Dmitri Vladimirovich Fursaev
Wladyslaw Chmielowski, Roman Zawodny
Marta Deperas-Kaminska, Katia Zaytseva