como utilizar o genbank
TRANSCRIPT
Bioinformática
COMO UTILIZAR O GENEBANKNCBI -National Center for Biotechnology Information
Costa de Oliveira, AntonioMaia, Luciano Carlos da
Centro de Genômica e FitomelhoramentoFAEM - Universidade Federal Pelotas
id1845656 pdfMachine by Broadgun Software - a great PDF writer! - a great PDF creator! - http://www.pdfmachine.com http://www.broadgun.com
BLAST - Basic Local Alignment Search ToolAltschul et al, 1990
BLASTn
Nucleotide query x Nucleoide DataBaseSearch 5� and/or reverse complement sequence
Exercicio 1
NCBI -National Center for Biotechnology Information
Exercicio 1: Como utilizar o BLAST no NCBI (on-line).BLASTn (nucleotideos x nucleotideos)
A. Dada uma sequencia de nucleotideos, verificar se existem sequencias homologase quais as informaçoes referenciadas para suas �homologias�
A1. Interpretando o E-valueA2. Interpretaçao detalhada dos alinhamentos com melhor E-value
A3. Existem informaçoes genomicas para os homologos?BACs?Genoma completo?
Qual a estrutura deste gene?promoterintronsexons (UTR, CDS)
A4. Existem informações de homologias em bancos de dados de regioesexpressas?
A5. Exitem informações sobre a validação experimental destes homologos?
ATGGGGGTGACGAAGGCGGAGGCGGTTGCCGCGACGGGGAAGGTGGTGGACGACATCGAGGCGCTCGCCGATCTCAGAAAGGAGCCAGCATGGAAAAAGTTTTTGTCCCACATTGGACCAGGCTTTATGGTCTGTTTGGCTTACCTCGATCCTGGAAATATGGAGACTGATCTACAAGCTGGTGCCAATCACAAATATGAGCTTCTTTGGGTGATTTTGATTGGCCTGATCTTTGCACTGATTATACAATCATTATCGGCTAATCTTGGAGTGGTGACAGGGCGCCATCTTGCTGAGCTGTGCAAGACTGAATATCCAGTATGGGTGAAAACCTGCTTATGGCTGCTAGCAGAACTAGCTGTGATTGCTTCCGATATTCCAGAAGTTATAGGTACAGGATTTGCTTTCAACCTTTTGTTCCACATACCAGTGTGGACCGGGGTTCTCATTGCTGGCTCTAGCACGCTTCTTCTTCTTGGACTGCAAAGATATGGGGTAAGGAAACTGGAGGTTGTGGTCGCGCTATTGGTGTTTGTCATGGCAGGGTGCTTCTTCGTGGAGATGAGCATAGTCAAGCCTCCGGTTAATGAGGTCCTTCAAGGATTATTCATCCCCAGGCTCAGTGGGCCCGGTGCCACAGGAGACTCCATTGCCCTTCTTGGGGCTCTTGTAATGCCGCACAATCTATTCTTGCACTCTGCCCTGGTGCTGTCGAGGAATACACCCGCATCAGCAAAAGGAATGAAGGATGCCTGTAGGTTCTTCCTCTTTGAGAGTGGGATAGCTCTTTTCGTGGCTCTGCTTGTCAACATTGCAATCATCTCTGTCTCCGGCACTGTATGTAATGCAACCAACCTTTCACCAGAAGATGCTGTAAAATGCAGCGACCTTACATTGGACTCCTCATCCTTCCTCCTCAGGAATGTGCTAGGAAAGTCTAGTGCGACAGTGTACGGTGTAGCACTCTTGGCTTCTGGACAGAGCTCGACCATTACTGGCACTTATGCTGGGCAATATGTTATGCAGGGGTTCCTAGACATCAAAATGAAACAGTGGCTTCGGAACCTGATGACACGCAGCATTGCCATTGTGCCTAGCTTAATCGTCTCCATCATTGGAGGGTCTAGTGGCGCCGGTCGTCTCATCGTCATTGCATCGATGATTCTATCCTTTGAGCTACCATTTGCTCTGATCCCTCTTCTCAAGTTCAGCAGCAGCAGCAACAAGATGGGTGAAAACAAGAACTCCATCTATATTGTTGGATTCTCCTGGGTGCTGGGGTTCGTCATCATCGGGATAAACATCTACTTCCTCAGCACAAAGCTGGTCGGCTGGATCCTCCACAACGCGCTCCCGACATTCGCCAACGTGCTCATCGGCATCGTGCTGTTCCCGCTCATGCTGCTCTACGTCGTCGCCGTGATCTACCTGACGTTCAGGAAGGACACCGTCAAGTTCGTGTCTCGTCGTGAGCTGCAGGCCGGCGACGACACCGAGAAAGCACAGGTAGCCACCTGCGTCGCCGACGAGGACAGCAAAG AGCCACCCGTGTAG ATGGGGGTGACGAAGGCGGAGGCGGTTGCCGCGACGGGGAAGGTGGTGGACGACATCGAGGCGCTCGCCGATCTCAGAAAGGAGCCAGCATGGAAAAAGTTTTTGTCCCACATTGGACCAGGCTTTATGGT
NCBI -National Center for Biotechnology Information
Exercicio 1: Como utilizar o BLAST no NCBI (on-line).BLASTn (nucleotideos x nucleotideos)
BLAST - Basic Local Alignment Search ToolAltschul et al, 1990
BLASTx
Nucleotide query x Protein DataBase (a.á)Search nucleotide sequence translated in all 6 reading frame
Exercicio 2
NCBI -National Center for Biotechnology Information
Exercicio 2: Como utilizar o BLAST no NCBI (on-line).BLASTx (nucleotideos x amino ácidos)
B. Dada uma sequencia de nucleotideos, verificar se existem sequencias proteicas(a.á) homologas e quais as informaçoes referenciadas para suas �homologias�
B1. Interpretando o E-valueB2. Interpretaçao detalhada dos alinhamentos com melhor E-value
B3. Existem informaçoes genomicas para os homologos?BACs?Genoma completo?
Qual a estrutura deste gene?promoterintronsexons (UTR, CDS)
B4. Existem informações de homologias em bancos de dados de regioesexpressas?
B5. Exitem informações sobre a validação experimental destes homologos?B6. Existem informação estrutural desta proteina? Cristalografia?
NCBI -National Center for Biotechnology Information
Exercicio 2: Como utilizar o BLAST no NCBI (on-line).BLASTx (nucleotideos x amino ácidos)
ATGGCGTCGAAGGAGGAGGTGGCCGTGGAGACGGTGGAGGGCGGAGCGGCGGCGGCGAAGGCGCCGTACTGGACCCGCCGCCGGCGCCGCTGCTGGACACGAGCGAGCTGGGTAAGTGGTCGCTGTACCGTGCCCTCATCGCGGAGTTCATGGCGACGCTCATCTTCCTCTACGTGAGCATCGCCACCGTGATCGGGTACAAGAACCAGAGGGCCACCGTCGACGCGTGCACCGGCGTCGGCTACCTCGGCGTGGCGTGGTCGTTCGGTGCCACCATATTCGTCCTCGTCTACTGCACCGGCGGCGTCTCCGGCGGGCACATCAACCCGGCGGTGACGCTGGGCCTCTTCTTCGGGCGGAAGCTGTCGCTCGTCCGCACCGTGCTGTACGTCGTGGCGCAGTGCCTCGGCGCCATCGCCGGCGCCGGCATCGTCGGCACGTTCATCCTCGTCTACACCGTCTTCTCCGCCACCGACCCCAAGCGCACCGCCCGCGACTCCTTCATCCCCGTACTCGTGCCGCTGCCGATCGGGTTCGCGGTGTTCGTCGTGCACCTGGCGACGATTCCGATCACCGGCACGGGCATCAACCCGGCGAGGAGCCTCGGCGCCGCCGTGCTGTACAACCAGCACGCAGCTTGGAAAGACCACTGGATCTTCTGGGTGGGGCCGGTGATCGGGGCGTTCTTGGCGGCGGCGTACCACAAGCTGGTGCTGCGCGGCGAGGCCGCCAAGGCGCTCAGCTCGTTCAGGAGCACCAGCGTGACGGCGTGA
BLAST - Basic Local Alignment Search ToolAltschul et al, 1990
BLASTp
Protein (a.á) query x Protein (a.á) DataBase
Exercicio 3
NCBI -National Center for Biotechnology Information
Exercicio 3: Como utilizar o BLAST no NCBI (on-line).BLASTp (aminóácidox aminoácidos)
LGVPSVKHMFPFCDSSSPMDLPLYQQLQLSPSSPKTDQSSSFYC YPCSPPFAAADASFPLSYQIGSAAAADATPPQAVINSPDLPVQALMDHAPATATELGACASGAEGSGASLDRAAAAARKDRHSKICTAGGMRDRRMRLSLDVARKFFALQDMLGFDKASKTVQWLLNTSKSAIQEIMADDASSECVEDGSSSLSVDGKHNPAEQLGGGGDQKPKGNCRGEGKKPAKASKAAATPKPPRKSANNAHQVPDKETRAKARERARERTKEKHRMRWVKLASAIDVEAAAASGPSDRPSSNNLSHHSSLSMNMPCAAAELEERERCSSPLSNRSAGRMQEITGASDVVLGFGNGGGGYGDGGGNYYCQEQWELGGVVFQQNSRFY
BLAST - Basic Local Alignment Search ToolAltschul et al, 1990
TBLASTn
Protein (a.á) query x Nucleotide DataBaseTranslated in all Reading Frame
Exercicio 4
NCBI -National Center for Biotechnology Information
Exercicio 4: Como utilizar o BLAST no NCBI (on-line).TBLASTn (aminóácidox nucleotideos)
LGVPSVKHMFPFCDSSSPMDLPLYQQLQLSPSSPKTDQSSSFYC YPCSPPFAAADASFPLSYQIGSAAAADATPPQAVINSPDLPVQALMDHAPATATELGACASGAEGSGASLDRAAAAARKDRHSKICTAGGMRDRRMRLSLDVARKFFALQDMLGFDKASKTVQWLLNTSKSAIQEIMADDASSECVEDGSSSLSVDGKHNPAEQLGGGGDQKPKGNCRGEGKKPAKASKAAATPKPPRKSANNAHQVPDKETRAKARERARERTKEKHRMRWVKLASAIDVEAAAASGPSDRPSSNNLSHHSSLSMNMPCAAAELEERERCSSPLSNRSAGRMQEITGASDVVLGFGNGGGGYGDGGGNYYCQEQWELGGVVFQQNSRFY
BLAST - Basic Local Alignment Search ToolAltschul et al, 1990
TBLASTx
Nucleotide translated query x Nucleotide DataBase (Nucleotide translated query)Translated in 6 all Reading Frame x Translated in 6 all Reading Frame
Exercicio 5
NCBI -National Center for Biotechnology Information
Exercicio 5: Como utilizar o BLAST no NCBI (on-line).TBLASTn (aminóácidox nucleotideos)
ATGGAAACTGGCAACGTTGTGAATGCACAACCTGAATTATCAGGCATAATTGATGGATCCAAGAGCTATATGTACGAGCAGTTATGGAAACTCTGTGCGGGACCTTTGTGTGATATTCCAAAACTTGGAGAAAATGTTTATTACTTTCCTCAAGGCAATATAGAGCTTGTTGATGCATCTACAAGAGAAGAGTTGAATGAGTTACAACCAATTTGTGATCTTCCTTCTAAGCTTCAATGCCGTGTTATTGCAATTCATCTTAAGGTGGAAAACAATAGTGATGAGACTTATGCTGAGATCACTTTGATGCCAGATACAACTCAAGTTGTAATCCCTACTCAGAGTGAAAATCAATTTAGGCCATTAGTTAATTCCTTTACGAAGGTTTTAACGGCCTCTGATACAAGCGCTTATGGTGGATTCTTTGTTCCGAAAAAACATGCCATTGAATGTCTCCCTCCACTGCCTCTACCGGCACAAGAACTTCTTGCTAAAGATCTCCATGGTAATCAATGGAGATTCAGACACAGCTATAGAGGTACACCGCAAAGACATTCGTTAACAACTGGTTGGAATGAATTCACAACATCGAAAAAGTTGGTTAAAGGAGATGTTATTGTATTCGTTAGGGGAGAGACTGGAGAGTTACGAGTTGGTATCAGACGAGCAAGACATCAACAAGGGAATATACCTTCATCAATAGTATCAATAGATTGTATGAGACATGGGGTAATTGCTTCCGCAAAGCATGCTCTTGATAACCAATGTATATTCATTGTGGTTTACAAGCCAAGTATCAGGTCAAGTCAATTTATTGTCAGTTACGACAAATTCTTAGACGCTATGAACAATAAGTTCATTGTCGGTTCAAGATTTACAATGCGGTTTGAGGGTGATGATTTCTCTGAAAGAAGATATTTTGGGACAATTATTGGAGTTAATGATTTCTCTCCTCATTGGAAGTGTTCAGAGTGGCGCAGCTTAGAAGTTCAGTGGGATGAATTTGCATCATTTTCGAGACCTAATAAAGTGTCACCCTGGGAGATCGAACATTTAATGTCTGCGTTAAATGTTCCGCGATCATCTTTGCTCAAGAACAAGCGTTTACGAGAAGTTAATGAATTCGGACAAGAAATTGGACAACTAAGCGTCGCCTCCCCGATGAATACTTCTCTTCGTTATCGTGATACAACTGAAGATGCTATGAATCCTTCTAGATTGCTAATGAGCTACCCTGTCCAACCAATGCCCAAACTAAATTACAATAACCAAATGGTTACACAAATAGAAGAAAATATAACAACCAAGGCAGTCACTAATTTTAGGCTGTTTGGAGTCTCTCTAGCCATTCCTCTAGTGATCAAAGATCCCATTGAAGAAATTGGCTCGGATATTTCGAAACTTACTGAAGGAAAAAAGTTTGGTCAAAGCCAAACTTTGAGATCACCAATAGAGATCCAAAGCAAGCAGTTTGGTTCTACTAGAACTTGTACCAAAGTTCAAATGCAAGGTGTAACAATAGGAAGAGCTGTGGATTTAAGTGTTCTTAATGGATATGATCAGCTAATACTAGAATTGGAGAAGCTCTTTGATCTCAAAGGCCAACTACAAACTCGCAACCAATGGAAAATAGCTTTCACAGATAGTGACGGGTATGAGATGCTTGTTGGAGACGATCCATGGCCTGAATTCTGCAAAATGGTGAAGAAGATATTGATATATTCAAAAGAGGAGGTCAAAAACCTAAAGTCCAGTAAGAGTCTCTCAAGTTGA
??????????????
1) BUSCAR HOMOLOGIAS (BLAST)JULGAR QUAL SEQUENCIAS SAO AS MAIS PARECIDAS
2) DESCRICAO DA FUNÇÃO DO GENE/PROTEÍNA
3) EXISTE SEQUENCIAS CONFIRMANDO A EXPRESSÃO DE HOMOLOGOSDESTAS SEQUENCIAS??
UNIGENE
4) EXISTE INFORMAÇÃO DA VALIDAÇÃO EXPERIMENTAL DOS HOMOLOGOSDESTA SEQUENCIA? EM QUE ESPECIE? SAO RESPONSIVOS A QUAIS FATORES?
GEOPROFILE
5) EXPLIQUE COMO QUANTO E PARA QUE ESSE GENE PODE SER UTILIZADOCOMO MARCADOR MOLECULAR!!!
TRABALHO PARA PRÓXIMA AULA
RESULTADO DO TRABALHO:
1)OS DADOS SERAO APRESENTADOS NA PROXIMA AULA POR CADA DUPLA
2) APRESENTAR EM 15 MIN NO POWERPOINT
TRABALHO PARA PRÓXIMA AULA
Bioinformática
COMO UTILIZAR O GENEBANKNCBI -National Center for Biotechnology Information
Costa de Oliveira, AntonioMaia, Luciano Carlos da
Centro de Genômica e FitomelhoramentoFAEM - Universidade Federal Pelotas
id2118734 pdfMachine by Broadgun Software - a great PDF writer! - a great PDF creator! - http://www.pdfmachine.com http://www.broadgun.com
BLAST - Basic Local Alignment Search ToolAltschul et al, 1990
BLASTn
Nucleotide query x Nucleoide DataBaseSearch 5� and/or reverse complement sequence
Exercicio 1
NCBI -National Center for Biotechnology Information
Exercicio 1: Como utilizar o BLAST no NCBI (on-line).BLASTn (nucleotideos x nucleotideos)
A. Dada uma sequencia de nucleotideos, verificar se existem sequencias homologase quais as informaçoes referenciadas para suas �homologias�
A1. Interpretando o E-valueA2. Interpretaçao detalhada dos alinhamentos com melhor E-value
A3. Existem informaçoes genomicas para os homologos?BACs?Genoma completo?
Qual a estrutura deste gene?promoterintronsexons (UTR, CDS)
A4. Existem informações de homologias em bancos de dados de regioesexpressas?
A5. Exitem informações sobre a validação experimental destes homologos?
ATGGGGGTGACGAAGGCGGAGGCGGTTGCCGCGACGGGGAAGGTGGTGGACGACATCGAGGCGCTCGCCGATCTCAGAAAGGAGCCAGCATGGAAAAAGTTTTTGTCCCACATTGGACCAGGCTTTATGGTCTGTTTGGCTTACCTCGATCCTGGAAATATGGAGACTGATCTACAAGCTGGTGCCAATCACAAATATGAGCTTCTTTGGGTGATTTTGATTGGCCTGATCTTTGCACTGATTATACAATCATTATCGGCTAATCTTGGAGTGGTGACAGGGCGCCATCTTGCTGAGCTGTGCAAGACTGAATATCCAGTATGGGTGAAAACCTGCTTATGGCTGCTAGCAGAACTAGCTGTGATTGCTTCCGATATTCCAGAAGTTATAGGTACAGGATTTGCTTTCAACCTTTTGTTCCACATACCAGTGTGGACCGGGGTTCTCATTGCTGGCTCTAGCACGCTTCTTCTTCTTGGACTGCAAAGATATGGGGTAAGGAAACTGGAGGTTGTGGTCGCGCTATTGGTGTTTGTCATGGCAGGGTGCTTCTTCGTGGAGATGAGCATAGTCAAGCCTCCGGTTAATGAGGTCCTTCAAGGATTATTCATCCCCAGGCTCAGTGGGCCCGGTGCCACAGGAGACTCCATTGCCCTTCTTGGGGCTCTTGTAATGCCGCACAATCTATTCTTGCACTCTGCCCTGGTGCTGTCGAGGAATACACCCGCATCAGCAAAAGGAATGAAGGATGCCTGTAGGTTCTTCCTCTTTGAGAGTGGGATAGCTCTTTTCGTGGCTCTGCTTGTCAACATTGCAATCATCTCTGTCTCCGGCACTGTATGTAATGCAACCAACCTTTCACCAGAAGATGCTGTAAAATGCAGCGACCTTACATTGGACTCCTCATCCTTCCTCCTCAGGAATGTGCTAGGAAAGTCTAGTGCGACAGTGTACGGTGTAGCACTCTTGGCTTCTGGACAGAGCTCGACCATTACTGGCACTTATGCTGGGCAATATGTTATGCAGGGGTTCCTAGACATCAAAATGAAACAGTGGCTTCGGAACCTGATGACACGCAGCATTGCCATTGTGCCTAGCTTAATCGTCTCCATCATTGGAGGGTCTAGTGGCGCCGGTCGTCTCATCGTCATTGCATCGATGATTCTATCCTTTGAGCTACCATTTGCTCTGATCCCTCTTCTCAAGTTCAGCAGCAGCAGCAACAAGATGGGTGAAAACAAGAACTCCATCTATATTGTTGGATTCTCCTGGGTGCTGGGGTTCGTCATCATCGGGATAAACATCTACTTCCTCAGCACAAAGCTGGTCGGCTGGATCCTCCACAACGCGCTCCCGACATTCGCCAACGTGCTCATCGGCATCGTGCTGTTCCCGCTCATGCTGCTCTACGTCGTCGCCGTGATCTACCTGACGTTCAGGAAGGACACCGTCAAGTTCGTGTCTCGTCGTGAGCTGCAGGCCGGCGACGACACCGAGAAAGCACAGGTAGCCACCTGCGTCGCCGACGAGGACAGCAAAG AGCCACCCGTGTAG
NCBI -National Center for Biotechnology Information
Exercicio 1: Como utilizar o BLAST no NCBI (on-line).BLASTn (nucleotideos x nucleotideos)
BLAST - Basic Local Alignment Search ToolAltschul et al, 1990
BLASTx
Nucleotide query x Protein DataBase (a.á)Search nucleotide sequence translated in all 6 reading frame
Exercicio 2
NCBI -National Center for Biotechnology Information
Exercicio 2: Como utilizar o BLAST no NCBI (on-line).BLASTx (nucleotideos x amino ácidos)
B. Dada uma sequencia de nucleotideos, verificar se existem sequencias proteicas(a.á) homologas e quais as informaçoes referenciadas para suas �homologias�
B1. Interpretando o E-valueB2. Interpretaçao detalhada dos alinhamentos com melhor E-value
B3. Existem informaçoes genomicas para os homologos?BACs?Genoma completo?
Qual a estrutura deste gene?promoterintronsexons (UTR, CDS)
B4. Existem informações de homologias em bancos de dados de regioesexpressas?
B5. Exitem informações sobre a validação experimental destes homologos?B6. Existem informação estrutural desta proteina? Cristalografia?
NCBI -National Center for Biotechnology Information
Exercicio 2: Como utilizar o BLAST no NCBI (on-line).BLASTx (nucleotideos x amino ácidos)
ATGGCGTCGAAGGAGGAGGTGGCCGTGGAGACGGTGGAGGGCGGAGCGGCGGCGGCGAAGGCGCCGTACTGGACCCGCCGCCGGCGCCGCTGCTGGACACGAGCGAGCTGGGTAAGTGGTCGCTGTACCGTGCCCTCATCGCGGAGTTCATGGCGACGCTCATCTTCCTCTACGTGAGCATCGCCACCGTGATCGGGTACAAGAACCAGAGGGCCACCGTCGACGCGTGCACCGGCGTCGGCTACCTCGGCGTGGCGTGGTCGTTCGGTGCCACCATATTCGTCCTCGTCTACTGCACCGGCGGCGTCTCCGGCGGGCACATCAACCCGGCGGTGACGCTGGGCCTCTTCTTCGGGCGGAAGCTGTCGCTCGTCCGCACCGTGCTGTACGTCGTGGCGCAGTGCCTCGGCGCCATCGCCGGCGCCGGCATCGTCGGCACGTTCATCCTCGTCTACACCGTCTTCTCCGCCACCGACCCCAAGCGCACCGCCCGCGACTCCTTCATCCCCGTACTCGTGCCGCTGCCGATCGGGTTCGCGGTGTTCGTCGTGCACCTGGCGACGATTCCGATCACCGGCACGGGCATCAACCCGGCGAGGAGCCTCGGCGCCGCCGTGCTGTACAACCAGCACGCAGCTTGGAAAGACCACTGGATCTTCTGGGTGGGGCCGGTGATCGGGGCGTTCTTGGCGGCGGCGTACCACAAGCTGGTGCTGCGCGGCGAGGCCGCCAAGGCGCTCAGCTCGTTCAGGAGCACCAGCGTGACGGCGTGA
BLAST - Basic Local Alignment Search ToolAltschul et al, 1990
BLASTp
Protein (a.á) query x Protein (a.á) DataBase
Exercicio 3
NCBI -National Center for Biotechnology Information
Exercicio 3: Como utilizar o BLAST no NCBI (on-line).BLASTp (aminóácidox aminoácidos)
LGVPSVKHMFPFCDSSSPMDLPLYQQLQLSPSSPKTDQSSSFYC YPCSPPFAAADASFPLSYQIGSAAAADATPPQAVINSPDLPVQALMDHAPATATELGACASGAEGSGASLDRAAAAARKDRHSKICTAGGMRDRRMRLSLDVARKFFALQDMLGFDKASKTVQWLLNTSKSAIQEIMADDASSECVEDGSSSLSVDGKHNPAEQLGGGGDQKPKGNCRGEGKKPAKASKAAATPKPPRKSANNAHQVPDKETRAKARERARERTKEKHRMRWVKLASAIDVEAAAASGPSDRPSSNNLSHHSSLSMNMPCAAAELEERERCSSPLSNRSAGRMQEITGASDVVLGFGNGGGGYGDGGGNYYCQEQWELGGVVFQQNSRFY
BLAST - Basic Local Alignment Search ToolAltschul et al, 1990
TBLASTn
Protein (a.á) query x Nucleotide DataBaseTranslated in all Reading Frame
Exercicio 4
NCBI -National Center for Biotechnology Information
Exercicio 4: Como utilizar o BLAST no NCBI (on-line).TBLASTn (aminóácidox nucleotideos)
LGVPSVKHMFPFCDSSSPMDLPLYQQLQLSPSSPKTDQSSSFYC YPCSPPFAAADASFPLSYQIGSAAAADATPPQAVINSPDLPVQALMDHAPATATELGACASGAEGSGASLDRAAAAARKDRHSKICTAGGMRDRRMRLSLDVARKFFALQDMLGFDKASKTVQWLLNTSKSAIQEIMADDASSECVEDGSSSLSVDGKHNPAEQLGGGGDQKPKGNCRGEGKKPAKASKAAATPKPPRKSANNAHQVPDKETRAKARERARERTKEKHRMRWVKLASAIDVEAAAASGPSDRPSSNNLSHHSSLSMNMPCAAAELEERERCSSPLSNRSAGRMQEITGASDVVLGFGNGGGGYGDGGGNYYCQEQWELGGVVFQQNSRFY
BLAST - Basic Local Alignment Search ToolAltschul et al, 1990
TBLASTx
Nucleotide translated query x Nucleotide DataBase (Nucleotide translated query)Translated in 6 all Reading Frame x Translated in 6 all Reading Frame
Exercicio 5
NCBI -National Center for Biotechnology Information
Exercicio 5: Como utilizar o BLAST no NCBI (on-line).TBLASTn (aminóácidox nucleotideos)
ATGGAAACTGGCAACGTTGTGAATGCACAACCTGAATTATCAGGCATAATTGATGGATCCAAGAGCTATATGTACGAGCAGTTATGGAAACTCTGTGCGGGACCTTTGTGTGATATTCCAAAACTTGGAGAAAATGTTTATTACTTTCCTCAAGGCAATATAGAGCTTGTTGATGCATCTACAAGAGAAGAGTTGAATGAGTTACAACCAATTTGTGATCTTCCTTCTAAGCTTCAATGCCGTGTTATTGCAATTCATCTTAAGGTGGAAAACAATAGTGATGAGACTTATGCTGAGATCACTTTGATGCCAGATACAACTCAAGTTGTAATCCCTACTCAGAGTGAAAATCAATTTAGGCCATTAGTTAATTCCTTTACGAAGGTTTTAACGGCCTCTGATACAAGCGCTTATGGTGGATTCTTTGTTCCGAAAAAACATGCCATTGAATGTCTCCCTCCACTGCCTCTACCGGCACAAGAACTTCTTGCTAAAGATCTCCATGGTAATCAATGGAGATTCAGACACAGCTATAGAGGTACACCGCAAAGACATTCGTTAACAACTGGTTGGAATGAATTCACAACATCGAAAAAGTTGGTTAAAGGAGATGTTATTGTATTCGTTAGGGGAGAGACTGGAGAGTTACGAGTTGGTATCAGACGAGCAAGACATCAACAAGGGAATATACCTTCATCAATAGTATCAATAGATTGTATGAGACATGGGGTAATTGCTTCCGCAAAGCATGCTCTTGATAACCAATGTATATTCATTGTGGTTTACAAGCCAAGTATCAGGTCAAGTCAATTTATTGTCAGTTACGACAAATTCTTAGACGCTATGAACAATAAGTTCATTGTCGGTTCAAGATTTACAATGCGGTTTGAGGGTGATGATTTCTCTGAAAGAAGATATTTTGGGACAATTATTGGAGTTAATGATTTCTCTCCTCATTGGAAGTGTTCAGAGTGGCGCAGCTTAGAAGTTCAGTGGGATGAATTTGCATCATTTTCGAGACCTAATAAAGTGTCACCCTGGGAGATCGAACATTTAATGTCTGCGTTAAATGTTCCGCGATCATCTTTGCTCAAGAACAAGCGTTTACGAGAAGTTAATGAATTCGGACAAGAAATTGGACAACTAAGCGTCGCCTCCCCGATGAATACTTCTCTTCGTTATCGTGATACAACTGAAGATGCTATGAATCCTTCTAGATTGCTAATGAGCTACCCTGTCCAACCAATGCCCAAACTAAATTACAATAACCAAATGGTTACACAAATAGAAGAAAATATAACAACCAAGGCAGTCACTAATTTTAGGCTGTTTGGAGTCTCTCTAGCCATTCCTCTAGTGATCAAAGATCCCATTGAAGAAATTGGCTCGGATATTTCGAAACTTACTGAAGGAAAAAAGTTTGGTCAAAGCCAAACTTTGAGATCACCAATAGAGATCCAAAGCAAGCAGTTTGGTTCTACTAGAACTTGTACCAAAGTTCAAATGCAAGGTGTAACAATAGGAAGAGCTGTGGATTTAAGTGTTCTTAATGGATATGATCAGCTAATACTAGAATTGGAGAAGCTCTTTGATCTCAAAGGCCAACTACAAACTCGCAACCAATGGAAAATAGCTTTCACAGATAGTGACGGGTATGAGATGCTTGTTGGAGACGATCCATGGCCTGAATTCTGCAAAATGGTGAAGAAGATATTGATATATTCAAAAGAGGAGGTCAAAAACCTAAAGTCCAGTAAGAGTCTCTCAAGTTGA
??????????????
1) BUSCAR HOMOLOGIAS (BLAST)JULGAR QUAIS SEQUENCIAS SAO AS MAIS PARECIDAS!
2) DESCRICAO DA FUNÇÃO DO GENE/PROTEÍNA
3) EXISTEM SEQUENCIAS CONFIRMANDO A EXPRESSÃO PARA OS HOMOLÓGOS DESTA SEQUENCIA??
VER SE EXISTEM HOMOLÓGOS NO UNIGENE (cDNA)
4) EXISTEM INFORMAÇÕES DA VALIDAÇÃO EXPERIMENTAL DOS HOMOLOGOSDESTA SEQUENCIA? EM QUE ESPECIE? SÃO RESPONSIVOS A QUAIS FATORES?
GEOPROFILE
5) EXPLIQUE COMO, QUANTO E PARA QUE ESSE GENE (OU SEQUENCIA GENOMICA) PODERA SER UTILIZADO/A COMO MARCADOR MOLECULAR!!!
TRABALHO PARA PRÓXIMA AULA
RESULTADO DO TRABALHO:
1)OS DADOS SERÃO APRESENTADOS NA PRÓXIMA AULA POR CADA DUPLA
2) APRESENTAR EM 15 MINUTOS NO POWERPOINT
TRABALHO PARA PRÓXIMA AULA