chaur-tsuen lo ph. d. national formosa universitypolicies)no.3... · (b) production and formulation...
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Chaur-Tsuen Lo Ph. D.
National Formosa University
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Generally, the basic requirements of a successful system of biological control of plant pests are
(a) an effective biocontrol agent, (b) production and formulation methods that give
rise to high yields of biomass consisting of appropriate efficacious propagules of high viabilityand stability, and
(c) delivery systems that provide a conducive milieuand minimize growth of competitive microflora.
Harman, 1996
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何謂優良有效之生物體或菌株Whats is an effective biocontrol agent ?
Rhizosphere (phyllosphere) competence Promote plant growth Reduce plant diseases
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Promote plant growth
Negative control Root growth well
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CK Pa Pa+M Pa+Tr
Inhibit the pathogens and reduce the disease
1 nM
5 nM
10 nM
20 nM
EC
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Producing the high quality biomass with viability, vigor and stability materials
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木黴菌WP成品 木黴菌OL成品
木黴菌GG成品
Formulation
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傳輸體系 delivery system
Right thing(適當的材料或物質) Right form (適當的製劑或型態) Right time (適當的時機) Right target (適當的病原) Right dose (適當的用量) Right method (適當的方法)
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Crop(作物)
拮抗菌Biocontrol agent Pathogen病原菌
A successful biocontrol requires considerable understanding of cropping system; disease epidemiology; the biology, ecology, and population dynamics of biocontrol organisms; and the interactions among these variables.
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Microbial pesticides for insects
1. Entomopathogenic fungi
2. Baculoviruses
3. Bacillus thuringiensis (Bt)
Pheromones and attractants for insects
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Metarhizium anisopliae , Nomuraea rileyi , Beauveria bassiana and Verticillium lecanii were the frequent species in Taiwan.
Some species has been ready for use on insecticide
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Field trials
The larva of Trichoplusia ni was infected by Nomuraea rileyi in cabbage field.
The noctuid larva was infected by Beauveria bassiana after treated with B. bassiana spore suspension.
adding speader-sticker
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Solid-state fermenter
Oil-based recovery system developed for separation and concentration of hydrophobicconidia
Control of the紅胸葉蟲Brontispa longissima with M. anisopliae on華盛頓椰子Washingtonia filiferra
Liquid inoculation system
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Virulant baculoviruses have been isolated from lepidopterous insects.
Spodoptera exigua nuclear polyhedrosis virus, SeNPV
Spodoptera litura nuclear polyhedrosis virus, SlMNPV
Both laboratory pathogenicity tests and field efficacy trials were conducted with encouraging results.
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Green onion Spodoptera exigua
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核多角體病毒之開發利用開發昆蟲病毒量產技術,協助農友有效解決青蔥甜菜夜蛾及斜紋夜蛾等重要害蟲危害問題,使化學農藥減量使用,生產安全優質農產品
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Bacillus thuringiensis(Bt), is the most
popular microbial insecticide registered in
Taiwan , gave satisfactory results against some
agricultural insect pests and mosquitos.
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22Source: TACTRI
Screening of local Bt strains in Taiwan
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Pheromones and attractants
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Development and Application of Semiochemicals
Monitoring Mass trapping
Disruption
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Cylas formicanus elegantulus
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Development of Microbial Fungicides
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Bacillus subtilis
Bacillus amyloliquefacien
Trichoderma virens and harzianum
Streptomyces spp.
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Bacillus amyloliquefaciens Ba-BPD1 produces antibiotic substances, such as iturin and surfactin, has antimicrobial capacity for inhibiting the fungal or bacterial growth.
It is also a soil bacterium belonging to plant growth-promoting rhizobacteria (PGPR) with phosphate-solubilizing, siderphore and IAA production capability.
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To evaluate Ba-BPD1 as a biopesticide, several pathogens like Glomerella cingulata, Botrytis cinereaand so on, were chosen for bioassay, greenhouse potting test and field test, the result showed that Ba-BPD1 do have potential to develop as a bio-control agent.
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To evaluate Ba-BPD1 as a biofertilizer, cabbage and tomato were chosen for potting test, the result showed that Ba-BPD1 can improve the plant growth significantly, it indicated that Ba-BPD1 can not only control the disease of plant, it also can enhance the harvest of crops .
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Taiwan patent / United States Patent
Ba-BPD1
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Trichoderma harzianum
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Rhizosphere competence
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Trichoderma
Check
Check Trichoderma
Growth stages of lily
Primaryroots
Secondary root
Field trials and treatment
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Control root rot disease of melon
check Treated Trichoderma
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Bacillus thuringiensis subspecies kurstaki strain E-911
Technology Transfer and Registration before get into market
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Commercial products by different company in Taiwan
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Acknowledgements
Dr. Hsieh ,F-C provides several useful pictures
and some student’s technical support