Cell Structure & Function Inquiry Lab

Lab Packet

Stage

Stage clip

Light source

Body tube

Low-power objectiveRevolving nosepiece

Objective lenses

Ocular,Eyepiece

High-power objective

Base

Arm

Coarse adjustment knob

Fine adjustment knob

Diaphragm

1.

2.

3. 4.5.6.

7.

8.

9.10.

11.

12.

13.

14.

• Prepared Slides: slides that are purchased from a company and are labeled, stained, and ready for viewing. They DON’T require a cover slip. Used for viewing dead cells and organisms.

• Blank Slides: slides that you must prepare in order to view live specimens. Usually requires you to make a wet mount. Also REQUIRES a cover slip

Cover Slip

Blank Slide

Wet MountsMaking a Wet Mount• Obtain a blank slide• Use forceps or a pipette to

place your specimen in the middle of the slide

• Use pipette to add 1 drop of water (if specimen isn’t already contained in a liquid such as pond water)

• Cover with a cover slip

1

2

3 4

How to Focus a Microscope

1. Always start with Low Power• Place the slide on the stage and fasten with

stage clips• Rotate objective lens to low power (smallest)• Use course focus knob to bring image into focus

2. Medium Power• Rotate objective lens to medium power (2nd smallest

objective)• Use course focus knob to bring image into focus

3. High Power• Rotate objective lens to high power (largest objective)• Use FINE FOCUS knob to bring image into focus

NEVER USE COURSE FOCUS KNOB WHEN IN HIGH POWER BECAUSE IT CAN DAMAGE THE

MICROSOCOPE!!!!!

Cheek Cells

Yogurt Cells

YogurtBacteria

Bacterial Highly MagnifiedThey come in different shapes (Rod, Coccus) and may travel alone or in groups

Eukaryotic & Prokaryotic Cells

Cell Membrane

Animal Cell

Plant Cell

Parts 2 & 3

• In this section you will be using your smartphone and/or the class computers to look up information.

• Be sure to take notes on the internet sources you use because you will be REQUIRED TO CITE THEM in MLA format in Part 3

• Directions for MLA citations are on the next page.

MLA Format – In-text Citations• WEB SITES: There are times when some elements of information about a source will be

missing. For Web and periodical articles, an author’s name is sometimes omitted because the authorship is considered to be the organization hosting or publishing the article.

– For corporate authorship, include the name of the organization.• Example 1: When the polishing error was discovered, the Hubble mirror was described as “needing

glasses” (NASA 234).

– For Web sites that lack page numbers, do not make them up or use the page numbers on a printout. List only the author’s last name.

• Example 2: The award is based on the results of Web-based surveys, limiting the survey sample to those who have Internet access (Doe).

– No human or corporate author for a Web article, use an abbreviated title as the reference. Use the first part of the Web article title, so that it refers clearly to the title in the Works Cited. For an article titled, “New Research Shows Americans Getting Too Little Vitamin D,” the in-text citation might look like this:

• Example 3: New studies have concluded that Americans are getting an insufficient amount of Vitamin D, in part because of too little time spent outdoors (“New Research”).

– If you must list the website name in the signal phrase that appears before a quote in your text, - do not include URL addresses in-text. Provide partial URL’s, like MSNBC.com not http://www.msnbc.com.

Sperm Cell

Neuron

Root Cell

Parenchyma Cell

Bacillus Bacteria

Spirochete Bacteria

Euglena

Dinoflagellate