cell based assays presentation v3_03_2012
DESCRIPTION
Update presentation on "increasing returns in drug discovery by harnessing the power of cells". Includes images/data/pubs of differentiating human sensory and dopaminergic neurons from hNP1 neural progenitors + osteoblasts and chondrocytes from human mesenchymal stem cells. Our platforms are ideal for high throughput screening and other drug discovery processes.TRANSCRIPT
Increasing Returns in Drug Discovery
Harnessing the Power of CellsTM
Executive Overview
Focus:Provide pre-clinical development tools and methods to enable successful
outcomes in early phase drug discovery. Failure is success when determined early.*
*90% of lead candidates identified by current in vitro systems fail to become drugs
Value Summary: Physiologically relevant human stem-derived terminally differentiated
cells, cellular systems and primary cell lines
Better in vitro screening of targets and molecules = Reduced animal model use Shorten development timeline Lower downstream costs
*Expanding hN2 Primary Human Neurons stained with our chicken polyclonal antibody to Vimentin and DAPI (blue)
Executive Overview-V1-30-2013
MSCGro™ Media vs Alternative
Why Consider our Solutions Research proven solutions used by large Pharmas, Biotechs,
Academic and Government Labs. 2500+ customers Publications/Testimonials
Lower per well costs-we can aggressively discount cells and media
Customer success focused Expert technical support Detailed protocols and methods Quick replacements and reorders Large expert resource network
Published expert input Non confidential; customer feedback and data sharing
through social media outlets and blogs
Executive Overview-V2-30-2013
ApplicationsHigh throughput and high content screening
Compound Testing Toxicology Studies Discovery
Cell expansion, differentiation and migration studies
In Vivo cell Imaging
Images: To demonstrate the capabilities of the NIR-FLIVO® 747 apoptosis tracer, adult wild-type Balb/c mice were either inoculated with HSV-1 virus, which is known to induce apoptosis in the brain, or given a sham treatment. Seven days after viral inoculation, the mice were injected intravenously with either the NIR-FLIVO® 747apoptosis tracer (cat. KF17368), the NIR-FLIVO® 747 free dye (cat. KF17370, DyLight®747), or no reagent.
Images: (A) UCB Derived hMSCs cultured using our low Serum MSCGro media and then differentiated using our Oetsogenic Differentiation Media.
Executive Overview-V3-03-2013
Tools and Capabilities
Testimonial: got 10 million cells total after extraction from the tissue. At Day 4 they all developed long axons. Thank you so much for the replacement." Dr. Lidia Gardner, University of Tennessee HSC.
Human Mesenchymal Stem Cells-Green Fluorescent-Labeled
hDopamingergic Neurons Derived using hGNDF
Cell Based Assays Tools Include:
• Growth-Differentiation Factors
• High-Performance Media
• Transfection Kits
• Markers• 3-D
Fibers/ECMs
hNP1 Neural Progenitors-100% Confluency
hN2 Human Neurons (mixed) stained with MAP-2 , Courtesy ofDr. Gerry Shaw, UF
FITC LabeledhChondrocytes
FITC LabeledhOsteoblasts
3-D Solutions
Migration AssaysCourtesy of EssenBiosciences
Coming soon!SPIO-LabeledCells for in-vivoImaging
Executive Overview-V3-03-2013
Tools and Capabilities
Images: Phase contrast images of the cultures before and after the sensory neuron induction. A) hNP1 culture before sensory induction. B) hNP1 culture 10 days after sensory induction. C) hNP1 culture 30 days after sensory induction. Neuronal clusters and axonal bundles, which resemble rat DRG cell cultures, were typically observed. D) For comparison, an image of a rat embryonic DRG cell culture at 7 DIV is provided.
Executive Overview-V3-03-2013
Example hNP1 Neural Progenitors to Sensory Neurons: Xiufang Guo, Severo Spradling, Maria Stancescu, Stephen Lambert, James J. Hickman. Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1. Biomaterials, In Press, Corrected Proof,Mar 2013.doi:10.1016/j.biomaterials.2013.02.061.
TM
Tools and Capabilities
Key Product Category Links:
Antibodies-Markers-internally developed markers for neuroscience, immune response, cancer, Autoimmune disease and stem cell research
Apoptosis Research Reagents -includes: detection kits, antibodies and proteins
Proteins Recombinant and natural proteins-includes a wide selection of cell growth factors.
Transfection & Reagents-Gene Expression Analysis, engineered cell lines for custom applications
Cell Based Assay-Cells, Media and 3-D Solutions, stem cell potency/toxicity
Executive Overview-V2-30-2013
Competitive advantages
Human Stem cell-derived: less animal studies, highly reproducible, cost-effective, bulk cells/systems of in-vivo replicates as in-vitro, defined systems.
Expertise in Stem Cell Differentiation: Substantial experience in differentiation, trans-differentiation and characterization
NSC & MSC-derived cell systems: Neurons, DA and mixed, 5-HT, ACh, NE, GABA-nergic, etc. MSC-derived chondrocytes, osteoblasts and endothelial cells.
IPSC-based cell-line generation platform: The classic four-gene method of Grudin and Yamanaka, the 2012 Nobel Prize winners, can be replaced by over-expression of Oct 3/4 only in adult stem cells & Vitro Biopharma has a proprietary position (with licensing opportunities). IPSCs platform provides capacity for any human cell lineage.
Transfection Expertise: This allows us to provide custom engineered cell systems for HTP screening.
Cell labeling: Stem cell/derivative labeling by fluorescent, magnetic and nanoparticle-based probes. Ideal for in-vivo imaging, HTP and marker analysis.
Technical Support: Substantial technical support in all phases of projects.
Executive Overview-V2-30-2013
Our Approach
Clear Understanding of where Neuromics/Vitro Biopharma could have a positive impact.
More intense in vitro screening of compounds and targets. Better candidates for downstream processes. Tools for in vivo assays
Propose solutions based on this understanding
Follow up, tweak and improve
Pete Shuster CEO and Owner Direct Phone: 612-801-1007 [email protected]
Executive Overview-V2-30-2013