blood tube guide 08 2012 final

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DIRECTORATE of LABORATORY MEDICINE Blood Tube Guide April 2012 DLM Client Services 276 4062/4428 Clinical Biochemistry Lab 276 4375 Specialist Lab 276 4699 Duty Biochemist; Bleep 4375 Paediatric Core Laboratory 701 2233 Paediatric Duty Biochemist; 701 2255 Haematology Coag, Counts, Red cell 276 4030 Molecular Diagnostics 276 4809 Transfusion 276 4400 Immunology 276 6440 Microbiology 276 8788/8854/3961 Virology 276 8788/8854 Other Useful Numbers: Molecular Genetics Laboratory 276 -6122 Cytogenetics 76 6737 Transplantation Laboratory 276-6397 You can also visit DLM website at: www.cmft.nhs.uk/info-for-health-professionals/laboratory-medicine.aspx

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Page 1: Blood Tube Guide 08 2012 Final

DIRECTORATE of LABORATORY MEDICINE

Blood Tube Guide April 2012

DLM Client Services 276 4062/4428

Clinical Biochemistry

Lab 276 4375 Specialist Lab 276 4699

Duty Biochemist; Bleep 4375 Paediatric Core Laboratory 701 2233 Paediatric Duty Biochemist; 701 2255

Haematology Coag, Counts, Red cell 276 4030 Molecular Diagnostics 276 4809

Transfusion 276 4400

Immunology 276 6440

Microbiology 276 8788/8854/3961

Virology

276 8788/8854

Other Useful Numbers:

Molecular Genetics Laboratory

276 -6122 Cytogenetics

76 6737 Transplantation Laboratory

276-6397

You can also visit DLM website at:

www.cmft.nhs.uk/info-for-health-professionals/laboratory-medicine.aspx

Page 2: Blood Tube Guide 08 2012 Final

DIRECTORATE of LABORATORY MEDICINE

ABBREV

TEST DEPARTMENT TUBE TOP COLOUR FOR ADULTS

TUBE TOP COLOUR FOR PAEDIATRIC / NEONATAL

ACTH Biochemistry Green IN ICE CONTACT LAB

Orange 4.0 ml top

APTT Activated partial thromboplastin time

Haematology Light blue

ALT Alanine transaminase Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Albumin Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Aldosterone Biochemistry Green – urgent to lab White Plain Clotted 2.0ml ALP Alkaline phosphatase Biochemistry Yellow Orange 1.3ml or

Green Neonatal 0.8ml Alkaline phosphatase

isoenzymes Biochemistry Yellow or red Orange 1.3ml or

Green Neonatal 0.8ml AFP Alphafetoprotein Biochemistry Yellow Orange 1.3ml or

Green Neonatal 0.8ml Alpha-1 Antitrypsin Biochemistry Yellow White Plain Clotted 2.0ml Amiodarone Biochemistry Yellow White Plain Clotted 2.0ml Ammonia Biochemistry Purple

IN ICE. CONTACT LAB*.

Red top 1.3ml or EDTA

Amitriptyline Biochemistry Yellow White Plain Clotted 2.0ml Amylase Biochemistry Yellow Orange 1.3ml or

Green Neonatal 0.8ml Amylase isoenzymes Biochemistry Yellow or red Orange 1.3ml or

Green Neonatal 0.8ml Androstenedione Biochemistry Yellow White Plain Clotted 2.0ml ACE Angeotensin converting

enzyme

Biochemistry Yellow White Plain Clotted 2.0ml

Anion-Gap Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

ENA Antibody to ENA Immunology Yellow

Anticoagulant Control Haematology Light blue

ANA Anti-nuclear antibody Immunology Yellow

Antioxidant profile Biochemistry CONTACT LAB BEFORE COLLECTION

Antithrombin Haematology Light blue

AP100 AP100 Immunology Red – URGENT TO LAB

APO-E genotype

Apolipoprotein-E genotype Biochemistry Purple

AST Aspartate transaminase Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

BCR-ABL Molecular Diagnostics Centre (Haematology)

4 x Purple

BHCG (Tumour Marker) Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Bicarbonate Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Bile acid Biochemistry Yellow

B12 Haematology Red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml

Page 3: Blood Tube Guide 08 2012 Final

DIRECTORATE of LABORATORY MEDICINE

Bilirubin Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Blood count Haematology Purple

Blood grouping+Antibody screen

Haematology Pink

Red Top

Bone Markers CTX ,PINP

Biochemistry Purple Yellow

Ca-125,Ca19-9,Ca15-3,CEA (tumour markers)

Biochemistry Yellow White Plain Clotted 2.0ml

C3 C3 Immunology Yellow

C4 C4 Immunology Yellow

CRP C Reactive protein Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Caeruloplasmin Biochemistry Yellow White Plastic Plain Clotted 5.0ml, always ordered with copper

Calcitonin Biochemistry Yellow – URGENT TO LAB ON ICE

White Plain Clotted 2.0ml

Calcium Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Carbamazepine Biochemistry Yellow White Plain Clotted 2.0ml

Carbohydrate deficient transferrin

Biochemistry Yellow

CD4 CD4 Immunology Purple

Cell Markers Immunology Purple

CH100 Immunology Red – URGENT TO LAB

Chloride Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Cholesterol Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Cholinesterase Biochemistry Yellow White Plain Clotted 2.0ml

Chromagranin A and B Biochemistry Purple

Chromosome Analysis Cytogenetics

CIH inhibitor Immunology Light blue URGENT TO LAB

Clomipramine Biochemistry Yellow

Coagulation disorders Haematology Light Blue

Coagulation screen Haematology Light Blue

Cold agglutinins screen Haematology Pink + purple at 37’C By arrangement olny

Copper Biochemistry Navy blue White Plastic Plain Clotted 5.0ml, usually ordered with caeruloplasmin unless for nutrition monitoring

C -peptide Biochemistry Yellow – URGENT TO LAB ON ICE

Orange 1.3ml or Green Neonatal 0.8ml

Cord blood investigations

Haematology 1 pink (maternal) 1 pink (cord)

Cortisol Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

CRP C-reactive protein Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Creatinine Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

CK Creatine kinase Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Cryoglobulins Immunology Red CONTACT LAB ext 6440

Page 4: Blood Tube Guide 08 2012 Final

DIRECTORATE of LABORATORY MEDICINE

CTX Crosslaps (bone marker) Biochemistry Purple

Cyclosporin Biochemistry Purple Purple top

D-dimer Haematology Light blue

11-deoxycortisol Biochemistry Yellow – KEEP ON ICE URGENT

White Plain Clotted 2.0ml

DHEA sulphate Biochemistry Yellow White Plain Clotted 2.0ml

DIC screen Haematology Light blue

Differential leucocyte count Haematology Purple

Digoxin Biochemistry Yellow White Plain Clotted 2.0ml

Direct Antibody Test (DAT) Haematology Pink Red Top

DNA studies Molecular Genetics Laboratory

Purple EDTA

Purple EDTA

Dothiepin Biochemistry Yellow

Down's Syndrome Screen Biochemistry Yellow

U&E Electrolytes Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

EP Electrophoresis Immunology Yellow

EOP Erythropotin Haematology Red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml

ESR Erythrocyte sedimentation rate

Haematology Purple Can be performed from blood count sample

Purple/ black top. Can be performed from blood count sample

Ethanol Biochemistry Grey or yellow Orange 1.3ml or Green Neonatal 0.8ml

Ethosuximide Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml

Ethylene glycol Biochemistry Yellow or Grey Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml

Factor V Leiden (& prothrombin) genotyping

Molecular Diagnostics Centre (Haematology)

Purple

Factor VIII Haematology Light blue

Factor assay (pre/post dose) Haematology Light blue

Ferritin Haematology Red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml

Fibrinogen estimation Haematology Light blue

FDP Fibrin degradation products (D-dimers)

Haematology Light blue

FK506 - Tacrolimus Biochemistry Purple Purple top

Flecainide Biochemistry Yellow

Folate (serum only) Haematology Red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml

Folate ( RBC) Haematology purple purple FSH Follicle Stim. Hormone Biochemistry Yellow Orange 1.3ml or

Green Neonatal 0.8ml free T4 Biochemistry Yellow Orange 1.3ml or

Green Neonatal 0.8ml FBC Full Blood Count (FBC) Haematology Purple

G6PD Haematology Purple

GGT Gamma-GT Biochemistry Yellow Orange 1.3ml or

Page 5: Blood Tube Guide 08 2012 Final

DIRECTORATE of LABORATORY MEDICINE

Green Neonatal 0.8ml

Genetic leuk Molecular Diagnostics Centre (Haematology)

4 x Purple

Gentamicin Biochemistry Red top Orange 1.3ml or Green Neonatal 0.8ml

GHRH Biochemistry Red

GIFN Immunology Special 3 bottles sent with patient

Glandular fever Haematology Purple

Glucose Biochemistry Grey Yellow top 1.0ml

GTT Glucose Tolerance Test Biochemistry Grey Yellow top 1.0ml

HbA1c Glycated Haemoglobin Biochemistry Purple Purple top

Growth Hormone Biochemistry Yellow White Plain Clotted 2.0ml

Gut hormone Biochemistry Bottle from lab KEEP ON ICE –URGENT*

Purple top on ice

Haematinics Haematology Red + purple

Haematocrit Haematology Purple

HFE Haemochromatosis genotyping

Molecular Diagnostics Centre (Haematology)

Light blue or purple

Haemoglobin Haematology Purple

Haemoglobin F Haematology Purple

Haemoglobin A2 Haematology Purple

Haemoglobinopathy Haematology 2 x 5ml Purple purple

HbA1c Biochemistry Purple Purple top

Haemolytics Haematology purple purple

Haemophilia genetics or mutation screen

Molecular Diagnostics Centre (Haematology)

4 x 5 ml Purple

Hams test Haematology Purple

Haptoglobin Haematology Red

HCG Human chorionic gonadotropin

Biochemistry Yellow

Heparin Haematology Light blue

HLA B27 Transplantation Lab Purple (EDTA tube) Purple (EDTA tube)

HLA B57 typing for Abacavir hypersensitivity

Transplantation Lab Purple (EDTA tube) Purple (EDTA tube)

HLA antibodies renal recipient Transplantation Lab Red Top tube (Plain). No anticoagulant

Red/White Top tube (Plain) No anticoagulant.

HLA typing HSCT patient Transplantation Lab Purple (EDTA tube) Purple (EDTA tube)

Homocysteine Biochemistry Grey NOTE TIME ON BOTTLE and send immediately to lab. for rapid separation.

Orange 1.3ml or Green Neonatal 0.8ml

Hydroxypcortisol (17-OH cortisol)

Biochemistry Purple or yellow

Imipramine Biochemistry Yellow

Immunoglobulins: Immunology Yellow

IgA/IgG/IgM Immunology Yellow

IgE – allergen specific Immunology Yellow

IgE – total Immunology Yellow

IGF1 IGF1 ( Insulin like growth factor 1)

Biochemistry Red Orange 1.3ml or Green Neonatal 0.8ml

Page 6: Blood Tube Guide 08 2012 Final

DIRECTORATE of LABORATORY MEDICINE

IgG/IgM antibody to ds-DNA Immunology Yellow

IgG/IgM anti-cardiolipin Immunology Yellow

IgG1/IgG2/IgG3/IgG4 subclass

Immunology Yellow

ID Immunodeficiency Immunology Purple

Immunofixation Immunology Yellow

Immunophenotyping Immunology Purple

Insulin Biochemistry Red- ON ICE + grey Orange 1.3ml or Green Neonatal 0.8ml ON ICE

Intrinsic factor antibodies Haematology Red

INR INR Haematology Light blue

Iron Saturation Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Iron Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Isoenzymes (Alkaline phosphatase/amylase iso-enymes)

Biochemistry Yellow or red Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml

Karotyping (chromosome analysis)

Molecular Genetics Laboratory

Green (lithium/Heparin) tube

KCT Haematology Light Blue

Kleihauer test Haematology Pink

Lactate Biochemistry Glucose tube (grey) SEND STRAIGHT TO LABORATORY

Yellow top 1.0ml

Lamotrigine Biochemistry Yellow White Plain Clotted 2.0ml

Lead levels Biochemistry Purple Red top 1.3ml PLASTIC EDTA

LH Luteinising Hormone Biochemistry Light green Orange 1.3ml or Green Neonatal 0.8ml

Lipids Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Lithium Biochemistry Yellow White Plain Clotted 2.0ml

Long chain fatty acids Biochemistry Purple NOTE: this is not the same test as free fatty acids

Lupus anticoagulant Haematology 2 x Light blue

Lymphocyte subset Immunology Purple

Magnesium Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Malarial parasites Haematology Purple

MCT Mast cell tryptase Immunology Yellow NOTE TIME ON BOTTLES AND REQUEST CARD

MCH Haematology Purple

MCHC Mean cell haemoglobin concentration

Haematology Purple

MCV Mean cell volume (MCV) Haematology Purple

Page 7: Blood Tube Guide 08 2012 Final

DIRECTORATE of LABORATORY MEDICINE

Mercury levels Biochemistry Purple

Methotrexate Biochemistry Yellow White Plain Clotted 2.0ml

Mitochrondial DNA Molecular Genetics Laboratory

Monospot (Paul Bunnell) Haematology Purple

Neural tube defect screen Biochemistry Yellow

Oestradiol Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Orosomucoid Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Osmolality Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

PINP Procollogen type 1 (bone marker)

Biochemistry Yellow

PIIINP/P3NP

Procollogen type 3 (liver fibrosis marker)

Biochemistry Yellow

Paul Bunnell test (Monospot) Haematology Purple

Paracetamol Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

PTH Parathyroid hormone Biochemistry Purple Purple top

PTHrp PTH related peptide Biochemistry Contact lab for special tube

Purple top

PCR/ viral load Virology Purple EDTA

Phenobarbitone Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml

Phenytoin Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml

Phosphate Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Plasma Metadrenalines Biochemistry Purple – URGENT TO LAB IMMEDIATELY

Plasma osmolality Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Plasma renin Biochemistry green sent immediately to lab

Purple top tube

Plasma viscosity Haematology 4 x purple

Platelet aggregation / function Haematology 6 x light blue (By appt)

Platelet aggregation/function + nucleotides

Haematology 8 x light blue (By appt)

Porphyrins Biochemistry Purple top + urine. Contact lab. Before collection and send straight to lab. Protecting samples from light.

Purple top + urine. Contact lab. Before collection and send straight to lab. Protecting samples from light.

Potassium Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Pregnancy testing (blood)

Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Page 8: Blood Tube Guide 08 2012 Final

DIRECTORATE of LABORATORY MEDICINE

Progesterone Biochemistry Yellow White Plain Clotted 2.0ml

Prolactin Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Propranolol Biochemistry Yellow

PSA, FPSA

Prostate Specific Antigen ( Free or Total)

Biochemistry Yellow

Protein S (free) Haematology 2 x Light blue

Protein C Haematology 2x Light blue

Protein - total Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Prothrombin time Haematology Light blue

Prothrombin (& Factor V Leiden) genotyping

Molecular Diagnostics Centre (Haematology)

1 x Purple

Quantiferon Immunology Contact LAB

QHCG Quantitative HCG (For Blood pregnancy test / ectopic pregnancy)

Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Renin Biochemistry dark green send immediately to lab (NOT on ice)

Purple

Reticulocytes count Haematology Purple Can be performed from blood count sample

RF Rheumatoid factor Immunology Yellow

Salicylate Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Selenium Pancreatic Laboratory Red White Plastic Plain Clotted 5.0ml

Serology bacteria / viral Virology Red

Serum and red cell folate Haematology Purple + red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml and purple top.

SHBG Biochemistry Yellow White Plain Clotted 2.0ml

Sickle test Haematology Purple

Sirolimus Biochemistry Purple Purple top

Sodium Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

FK506 Tacrolimus Biochemistry Purple Purple top

Testosterone Biochemistry Yellow White Plain Clotted 2.0ml

Thalassaemia Haematology Purple

Theophylline Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Thrombophilia screening

Haematology 2 x Light Blue

Thyroglobulins Biochemistry Yellow

TFT, T3, T4, TSH

Thyroid Function Test Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml

TPO Thyroid peroxidase Immunology Yellow

TIBC Total Iron Binding Capacity

Biochemistry Not available for adults, see transferrin saturation

Orange 1.3ml or Green Neonatal 0.8ml

Transferrin saturation Biochemistry Yellow

Page 9: Blood Tube Guide 08 2012 Final

DIRECTORATE of LABORATORY MEDICINE

Transferrin Biochemistry Yellow

Triglycerides Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

TropT Troponin T Biochemistry Yellow White Plain Clotted 2.0ml

Tumour markers

CA 15- 3,CA 19–9,CA 125, CEA

Biochemistry Yellow White Plain Clotted 2.0ml

Urate Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Urea Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Uric Acid Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml

Valproic Acid/Valproate Biochemistry Yellow White Plain Clotted 2.0ml

Virology Serology Virology Red

Virology – PCR/viral load Virology Purple

Vitamin B12 Haematology Red

Vitamin C Biochemistry 64067 prior to collection to alert lab to collect samples from OPD

2 x green

Vitamin D Biochemistry Yellow White Plain Clotted 2.0ml

Vitamin E Biochemistry Yellow or red White Plain Clotted 2.0ml

Von Willebrand Haematology 2 x light blue

Von Willebrand genetics or mutation screen

Molecular Diagnostics Centre (Haematology)

4 x light blue

Zinc Biochemistry Navy blue White Plastic Plain Clotted 5.0ml

Page 10: Blood Tube Guide 08 2012 Final

DIRECTORATE of LABORATORY MEDICINE

Coagulation Specimen Requirement Guide

Coagulation Test Blue Top 4.5ml

Number of Specimens

COAG Profile

DIC Screen (includes Fibrinogen and D-Dimer/FDP)

D-DIMER (FDP)

HEPARIN Control

INR

Any combination of these tests can be performed on 1

sample

DLE (Lupus Screen) 2

THROMBOPHILIA Screen (includes Anti-thrombin 3 AT111, Protein C, Protein S, Factor V Leiden F5L, Prothrombin Gene Variant PGV) 2

FACTOR Assays (multiple) 1

Von Willebrand Screen (includes Factor 8 assay F8C, Ristocetin Co Factor RCF, Von Willebrand antigen VWAG. 2

Platelet Aggregation/Nucleotides

By appointment only, please check with the coagulation laboratory. Ext 12123.

For any queries contact the Coagulation lab. On ext 12123.