blood tube guide 08 2012 final
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blood tube investigation guideTRANSCRIPT
DIRECTORATE of LABORATORY MEDICINE
Blood Tube Guide April 2012
DLM Client Services 276 4062/4428
Clinical Biochemistry
Lab 276 4375 Specialist Lab 276 4699
Duty Biochemist; Bleep 4375 Paediatric Core Laboratory 701 2233 Paediatric Duty Biochemist; 701 2255
Haematology Coag, Counts, Red cell 276 4030 Molecular Diagnostics 276 4809
Transfusion 276 4400
Immunology 276 6440
Microbiology 276 8788/8854/3961
Virology
276 8788/8854
Other Useful Numbers:
Molecular Genetics Laboratory
276 -6122 Cytogenetics
76 6737 Transplantation Laboratory
276-6397
You can also visit DLM website at:
www.cmft.nhs.uk/info-for-health-professionals/laboratory-medicine.aspx
DIRECTORATE of LABORATORY MEDICINE
ABBREV
TEST DEPARTMENT TUBE TOP COLOUR FOR ADULTS
TUBE TOP COLOUR FOR PAEDIATRIC / NEONATAL
ACTH Biochemistry Green IN ICE CONTACT LAB
Orange 4.0 ml top
APTT Activated partial thromboplastin time
Haematology Light blue
ALT Alanine transaminase Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Albumin Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Aldosterone Biochemistry Green – urgent to lab White Plain Clotted 2.0ml ALP Alkaline phosphatase Biochemistry Yellow Orange 1.3ml or
Green Neonatal 0.8ml Alkaline phosphatase
isoenzymes Biochemistry Yellow or red Orange 1.3ml or
Green Neonatal 0.8ml AFP Alphafetoprotein Biochemistry Yellow Orange 1.3ml or
Green Neonatal 0.8ml Alpha-1 Antitrypsin Biochemistry Yellow White Plain Clotted 2.0ml Amiodarone Biochemistry Yellow White Plain Clotted 2.0ml Ammonia Biochemistry Purple
IN ICE. CONTACT LAB*.
Red top 1.3ml or EDTA
Amitriptyline Biochemistry Yellow White Plain Clotted 2.0ml Amylase Biochemistry Yellow Orange 1.3ml or
Green Neonatal 0.8ml Amylase isoenzymes Biochemistry Yellow or red Orange 1.3ml or
Green Neonatal 0.8ml Androstenedione Biochemistry Yellow White Plain Clotted 2.0ml ACE Angeotensin converting
enzyme
Biochemistry Yellow White Plain Clotted 2.0ml
Anion-Gap Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
ENA Antibody to ENA Immunology Yellow
Anticoagulant Control Haematology Light blue
ANA Anti-nuclear antibody Immunology Yellow
Antioxidant profile Biochemistry CONTACT LAB BEFORE COLLECTION
Antithrombin Haematology Light blue
AP100 AP100 Immunology Red – URGENT TO LAB
APO-E genotype
Apolipoprotein-E genotype Biochemistry Purple
AST Aspartate transaminase Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
BCR-ABL Molecular Diagnostics Centre (Haematology)
4 x Purple
BHCG (Tumour Marker) Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Bicarbonate Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Bile acid Biochemistry Yellow
B12 Haematology Red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml
DIRECTORATE of LABORATORY MEDICINE
Bilirubin Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Blood count Haematology Purple
Blood grouping+Antibody screen
Haematology Pink
Red Top
Bone Markers CTX ,PINP
Biochemistry Purple Yellow
Ca-125,Ca19-9,Ca15-3,CEA (tumour markers)
Biochemistry Yellow White Plain Clotted 2.0ml
C3 C3 Immunology Yellow
C4 C4 Immunology Yellow
CRP C Reactive protein Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Caeruloplasmin Biochemistry Yellow White Plastic Plain Clotted 5.0ml, always ordered with copper
Calcitonin Biochemistry Yellow – URGENT TO LAB ON ICE
White Plain Clotted 2.0ml
Calcium Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Carbamazepine Biochemistry Yellow White Plain Clotted 2.0ml
Carbohydrate deficient transferrin
Biochemistry Yellow
CD4 CD4 Immunology Purple
Cell Markers Immunology Purple
CH100 Immunology Red – URGENT TO LAB
Chloride Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Cholesterol Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Cholinesterase Biochemistry Yellow White Plain Clotted 2.0ml
Chromagranin A and B Biochemistry Purple
Chromosome Analysis Cytogenetics
CIH inhibitor Immunology Light blue URGENT TO LAB
Clomipramine Biochemistry Yellow
Coagulation disorders Haematology Light Blue
Coagulation screen Haematology Light Blue
Cold agglutinins screen Haematology Pink + purple at 37’C By arrangement olny
Copper Biochemistry Navy blue White Plastic Plain Clotted 5.0ml, usually ordered with caeruloplasmin unless for nutrition monitoring
C -peptide Biochemistry Yellow – URGENT TO LAB ON ICE
Orange 1.3ml or Green Neonatal 0.8ml
Cord blood investigations
Haematology 1 pink (maternal) 1 pink (cord)
Cortisol Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
CRP C-reactive protein Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Creatinine Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
CK Creatine kinase Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Cryoglobulins Immunology Red CONTACT LAB ext 6440
DIRECTORATE of LABORATORY MEDICINE
CTX Crosslaps (bone marker) Biochemistry Purple
Cyclosporin Biochemistry Purple Purple top
D-dimer Haematology Light blue
11-deoxycortisol Biochemistry Yellow – KEEP ON ICE URGENT
White Plain Clotted 2.0ml
DHEA sulphate Biochemistry Yellow White Plain Clotted 2.0ml
DIC screen Haematology Light blue
Differential leucocyte count Haematology Purple
Digoxin Biochemistry Yellow White Plain Clotted 2.0ml
Direct Antibody Test (DAT) Haematology Pink Red Top
DNA studies Molecular Genetics Laboratory
Purple EDTA
Purple EDTA
Dothiepin Biochemistry Yellow
Down's Syndrome Screen Biochemistry Yellow
U&E Electrolytes Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
EP Electrophoresis Immunology Yellow
EOP Erythropotin Haematology Red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml
ESR Erythrocyte sedimentation rate
Haematology Purple Can be performed from blood count sample
Purple/ black top. Can be performed from blood count sample
Ethanol Biochemistry Grey or yellow Orange 1.3ml or Green Neonatal 0.8ml
Ethosuximide Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml
Ethylene glycol Biochemistry Yellow or Grey Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml
Factor V Leiden (& prothrombin) genotyping
Molecular Diagnostics Centre (Haematology)
Purple
Factor VIII Haematology Light blue
Factor assay (pre/post dose) Haematology Light blue
Ferritin Haematology Red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml
Fibrinogen estimation Haematology Light blue
FDP Fibrin degradation products (D-dimers)
Haematology Light blue
FK506 - Tacrolimus Biochemistry Purple Purple top
Flecainide Biochemistry Yellow
Folate (serum only) Haematology Red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml
Folate ( RBC) Haematology purple purple FSH Follicle Stim. Hormone Biochemistry Yellow Orange 1.3ml or
Green Neonatal 0.8ml free T4 Biochemistry Yellow Orange 1.3ml or
Green Neonatal 0.8ml FBC Full Blood Count (FBC) Haematology Purple
G6PD Haematology Purple
GGT Gamma-GT Biochemistry Yellow Orange 1.3ml or
DIRECTORATE of LABORATORY MEDICINE
Green Neonatal 0.8ml
Genetic leuk Molecular Diagnostics Centre (Haematology)
4 x Purple
Gentamicin Biochemistry Red top Orange 1.3ml or Green Neonatal 0.8ml
GHRH Biochemistry Red
GIFN Immunology Special 3 bottles sent with patient
Glandular fever Haematology Purple
Glucose Biochemistry Grey Yellow top 1.0ml
GTT Glucose Tolerance Test Biochemistry Grey Yellow top 1.0ml
HbA1c Glycated Haemoglobin Biochemistry Purple Purple top
Growth Hormone Biochemistry Yellow White Plain Clotted 2.0ml
Gut hormone Biochemistry Bottle from lab KEEP ON ICE –URGENT*
Purple top on ice
Haematinics Haematology Red + purple
Haematocrit Haematology Purple
HFE Haemochromatosis genotyping
Molecular Diagnostics Centre (Haematology)
Light blue or purple
Haemoglobin Haematology Purple
Haemoglobin F Haematology Purple
Haemoglobin A2 Haematology Purple
Haemoglobinopathy Haematology 2 x 5ml Purple purple
HbA1c Biochemistry Purple Purple top
Haemolytics Haematology purple purple
Haemophilia genetics or mutation screen
Molecular Diagnostics Centre (Haematology)
4 x 5 ml Purple
Hams test Haematology Purple
Haptoglobin Haematology Red
HCG Human chorionic gonadotropin
Biochemistry Yellow
Heparin Haematology Light blue
HLA B27 Transplantation Lab Purple (EDTA tube) Purple (EDTA tube)
HLA B57 typing for Abacavir hypersensitivity
Transplantation Lab Purple (EDTA tube) Purple (EDTA tube)
HLA antibodies renal recipient Transplantation Lab Red Top tube (Plain). No anticoagulant
Red/White Top tube (Plain) No anticoagulant.
HLA typing HSCT patient Transplantation Lab Purple (EDTA tube) Purple (EDTA tube)
Homocysteine Biochemistry Grey NOTE TIME ON BOTTLE and send immediately to lab. for rapid separation.
Orange 1.3ml or Green Neonatal 0.8ml
Hydroxypcortisol (17-OH cortisol)
Biochemistry Purple or yellow
Imipramine Biochemistry Yellow
Immunoglobulins: Immunology Yellow
IgA/IgG/IgM Immunology Yellow
IgE – allergen specific Immunology Yellow
IgE – total Immunology Yellow
IGF1 IGF1 ( Insulin like growth factor 1)
Biochemistry Red Orange 1.3ml or Green Neonatal 0.8ml
DIRECTORATE of LABORATORY MEDICINE
IgG/IgM antibody to ds-DNA Immunology Yellow
IgG/IgM anti-cardiolipin Immunology Yellow
IgG1/IgG2/IgG3/IgG4 subclass
Immunology Yellow
ID Immunodeficiency Immunology Purple
Immunofixation Immunology Yellow
Immunophenotyping Immunology Purple
Insulin Biochemistry Red- ON ICE + grey Orange 1.3ml or Green Neonatal 0.8ml ON ICE
Intrinsic factor antibodies Haematology Red
INR INR Haematology Light blue
Iron Saturation Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Iron Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Isoenzymes (Alkaline phosphatase/amylase iso-enymes)
Biochemistry Yellow or red Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml
Karotyping (chromosome analysis)
Molecular Genetics Laboratory
Green (lithium/Heparin) tube
KCT Haematology Light Blue
Kleihauer test Haematology Pink
Lactate Biochemistry Glucose tube (grey) SEND STRAIGHT TO LABORATORY
Yellow top 1.0ml
Lamotrigine Biochemistry Yellow White Plain Clotted 2.0ml
Lead levels Biochemistry Purple Red top 1.3ml PLASTIC EDTA
LH Luteinising Hormone Biochemistry Light green Orange 1.3ml or Green Neonatal 0.8ml
Lipids Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Lithium Biochemistry Yellow White Plain Clotted 2.0ml
Long chain fatty acids Biochemistry Purple NOTE: this is not the same test as free fatty acids
Lupus anticoagulant Haematology 2 x Light blue
Lymphocyte subset Immunology Purple
Magnesium Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Malarial parasites Haematology Purple
MCT Mast cell tryptase Immunology Yellow NOTE TIME ON BOTTLES AND REQUEST CARD
MCH Haematology Purple
MCHC Mean cell haemoglobin concentration
Haematology Purple
MCV Mean cell volume (MCV) Haematology Purple
DIRECTORATE of LABORATORY MEDICINE
Mercury levels Biochemistry Purple
Methotrexate Biochemistry Yellow White Plain Clotted 2.0ml
Mitochrondial DNA Molecular Genetics Laboratory
Monospot (Paul Bunnell) Haematology Purple
Neural tube defect screen Biochemistry Yellow
Oestradiol Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Orosomucoid Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Osmolality Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
PINP Procollogen type 1 (bone marker)
Biochemistry Yellow
PIIINP/P3NP
Procollogen type 3 (liver fibrosis marker)
Biochemistry Yellow
Paul Bunnell test (Monospot) Haematology Purple
Paracetamol Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
PTH Parathyroid hormone Biochemistry Purple Purple top
PTHrp PTH related peptide Biochemistry Contact lab for special tube
Purple top
PCR/ viral load Virology Purple EDTA
Phenobarbitone Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml
Phenytoin Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml
Phosphate Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Plasma Metadrenalines Biochemistry Purple – URGENT TO LAB IMMEDIATELY
Plasma osmolality Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Plasma renin Biochemistry green sent immediately to lab
Purple top tube
Plasma viscosity Haematology 4 x purple
Platelet aggregation / function Haematology 6 x light blue (By appt)
Platelet aggregation/function + nucleotides
Haematology 8 x light blue (By appt)
Porphyrins Biochemistry Purple top + urine. Contact lab. Before collection and send straight to lab. Protecting samples from light.
Purple top + urine. Contact lab. Before collection and send straight to lab. Protecting samples from light.
Potassium Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Pregnancy testing (blood)
Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
DIRECTORATE of LABORATORY MEDICINE
Progesterone Biochemistry Yellow White Plain Clotted 2.0ml
Prolactin Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Propranolol Biochemistry Yellow
PSA, FPSA
Prostate Specific Antigen ( Free or Total)
Biochemistry Yellow
Protein S (free) Haematology 2 x Light blue
Protein C Haematology 2x Light blue
Protein - total Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Prothrombin time Haematology Light blue
Prothrombin (& Factor V Leiden) genotyping
Molecular Diagnostics Centre (Haematology)
1 x Purple
Quantiferon Immunology Contact LAB
QHCG Quantitative HCG (For Blood pregnancy test / ectopic pregnancy)
Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Renin Biochemistry dark green send immediately to lab (NOT on ice)
Purple
Reticulocytes count Haematology Purple Can be performed from blood count sample
RF Rheumatoid factor Immunology Yellow
Salicylate Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Selenium Pancreatic Laboratory Red White Plastic Plain Clotted 5.0ml
Serology bacteria / viral Virology Red
Serum and red cell folate Haematology Purple + red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml and purple top.
SHBG Biochemistry Yellow White Plain Clotted 2.0ml
Sickle test Haematology Purple
Sirolimus Biochemistry Purple Purple top
Sodium Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
FK506 Tacrolimus Biochemistry Purple Purple top
Testosterone Biochemistry Yellow White Plain Clotted 2.0ml
Thalassaemia Haematology Purple
Theophylline Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Thrombophilia screening
Haematology 2 x Light Blue
Thyroglobulins Biochemistry Yellow
TFT, T3, T4, TSH
Thyroid Function Test Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml
TPO Thyroid peroxidase Immunology Yellow
TIBC Total Iron Binding Capacity
Biochemistry Not available for adults, see transferrin saturation
Orange 1.3ml or Green Neonatal 0.8ml
Transferrin saturation Biochemistry Yellow
DIRECTORATE of LABORATORY MEDICINE
Transferrin Biochemistry Yellow
Triglycerides Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
TropT Troponin T Biochemistry Yellow White Plain Clotted 2.0ml
Tumour markers
CA 15- 3,CA 19–9,CA 125, CEA
Biochemistry Yellow White Plain Clotted 2.0ml
Urate Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Urea Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Uric Acid Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml
Valproic Acid/Valproate Biochemistry Yellow White Plain Clotted 2.0ml
Virology Serology Virology Red
Virology – PCR/viral load Virology Purple
Vitamin B12 Haematology Red
Vitamin C Biochemistry 64067 prior to collection to alert lab to collect samples from OPD
2 x green
Vitamin D Biochemistry Yellow White Plain Clotted 2.0ml
Vitamin E Biochemistry Yellow or red White Plain Clotted 2.0ml
Von Willebrand Haematology 2 x light blue
Von Willebrand genetics or mutation screen
Molecular Diagnostics Centre (Haematology)
4 x light blue
Zinc Biochemistry Navy blue White Plastic Plain Clotted 5.0ml
DIRECTORATE of LABORATORY MEDICINE
Coagulation Specimen Requirement Guide
Coagulation Test Blue Top 4.5ml
Number of Specimens
COAG Profile
DIC Screen (includes Fibrinogen and D-Dimer/FDP)
D-DIMER (FDP)
HEPARIN Control
INR
Any combination of these tests can be performed on 1
sample
DLE (Lupus Screen) 2
THROMBOPHILIA Screen (includes Anti-thrombin 3 AT111, Protein C, Protein S, Factor V Leiden F5L, Prothrombin Gene Variant PGV) 2
FACTOR Assays (multiple) 1
Von Willebrand Screen (includes Factor 8 assay F8C, Ristocetin Co Factor RCF, Von Willebrand antigen VWAG. 2
Platelet Aggregation/Nucleotides
By appointment only, please check with the coagulation laboratory. Ext 12123.
For any queries contact the Coagulation lab. On ext 12123.