banea ioana. within hospitals, a proper decontamination of surfaces and hands of the staff is...
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Banea Ioana
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within hospitals, a proper decontamination of surfaces and hands of the staff is mandatory, considering the need of nosocomial infections prevention Disinfectants – strong bactericidal activity
Used on surfaces Antiseptics – low bactericidal/bacteriostatic activity
Used on hands, lesions
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The comparison of antibacterial activity of various antiseptics and disinfectants that are commonly used in hospitals
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we analysed the activity of four type of antiseptics
Clorhexin Pliwa derm Iodine Alcohol
four disinfectants Triton rapid Pliwa big extra Roditzin Pliwa lemon fresh
On seven different bacterial species
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Serial binary dilutions
(100 μl)
Decontam
inants
(200 μl)
standard CLSI microdilution method. In microtiter plates, binary
dilutions were made of each decontaminant to a final volume of 100 μl.
0.5 McFarland bacterial suspensions
10 μl were inoculated in 9990 μl Muller-Hinton broth.
100 μl were inoculated into each of the plate wells.
incubated at 35°C for 24 hours.
0.5 McFarland 9990 μl M-H
10 μl
100 μl
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For each bacterial species and for each decontaminant, the last dilution were no growth was found was noted.
MIC
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From the last three dilutions without growth > spot inoculation
MBC
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S. aureus E. faecalis E. coliP.
aeruginosaShigella Salmonella Clostridium
Clorhexin 32 4 16 2 16 4 32
Pliwa derm 32 4 4 16 8 8 64
Iodine 128 128 64 64 32 64 256
Alcohol 8 4 4 16 4 8 4
Triton rapid 256 4096 256 256 4096 4096 4096
Pliwa big extra 4096 4096 512 512 4096 4096 4096
Roditzin 256 16 16 32 8 16 256
Pliwa lemon fresh
512 512 128 16 256 256 512
The highest the dilution factor …•The strongest the decontaminant•More sensitive bacteria
Anti
sep
tics
Dis
infe
ctants
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Rank Name Type Component 1 Component 2 Componenet 3
1 Pliwa big extra disinfectant amine Quaternary ammonium salt polyether
2 Triton rapid disinfectant amine polieter
3 Pliwa lemon fresh disinfectant alcohol Quaternary ammonium salt
4 Iod antiseptic iodine
5 Roditzin disinfectant amine alcohol, phosphates enzymes, surfactants
6 Pliwa derm antiseptic alcohol
7 Clorhexin antiseptic alcohol clorhexidin digluconate
8 Alcool antiseptic alcohol
Pote
ncy
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To disinfectants Pseudomonas aeruginosa Escherichia coli
To antiseptics Pseudomonas aeruginosa Escherichia coli Shigella Salmonella Gram-negative
bacteria
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The disinfectants have strong antibacterial activity there are some bacterial species which resist better than
other, especially if these substances are diluted too much.
The non-alcohol based decontaminants are the most potent For the decontamination of skin, other antiseptics than
alcohol are recommended.
The most resistant bacteria are the Gram-negative
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