“how to do a good experiment” · 2018-04-01 · 2) isolate your variable(s) good scientific...
TRANSCRIPT
“How to do a Good Experiment”
Marc S. Mendonca, Ph.D.
Since before the time of the Ancient Greeks, thousands of years ago, people have tried to find out more about the world around them, wondering how
and why things work.
Scientists come up with many great ideas to show how things work, but for an idea to become accepted, it has to be tested.
The tool scientists use to test their theories is called the scientific method. Whether you are studying stars, caterpillars or medicines, this method
remains the same.
If you have an idea, or a question, you have to be able to prove it and give evidence so that other scientists can check and test your results.
Full reference:Martyn Shuttleworth (Mar 19, 2008). How to Conduct Science Experiments. Retrieved from Explorable.com: https://explorable.com/conduct-science-experiments
1) Pick a Scientific Topic
Experiments whose results cause
sweeping scientific paradigm
shifts are very, very rare.
(Sorry)
The vast majority of experiments
answer small (focused), specific
questions.
Science is built upon the
accumulation of data from
countless experiments.
Pick a topic or an unanswered
question with a small, testable
scope.
Science moves really fast!
Serious scientific research requires you do
extensive background reading (before you plan
your experiments). Deep Dive Into the Literature
Have past experiments (i.e. the published literature)
answered the question you want to study? If yes,
then what questions have been left unanswered in
your area of research interest?
What is your overarching Hypothesis?
(The BIG IDEA)
Narrow your research focus to one testable problem
at a time!
Deep Dive Into the Literature
• Actively read a book(s) that surveys the entire field that can act as a
jumping-off point
• Talk to people (e.g. your advisor, postdocs) who are well-versed in the
field and get their suggestions for what to look at
• Use technology… Find all-around resources like Wikipedia and use
those as jumping-off points
• For academic reading, focus on reading review articles which
summarize findings
GO TO SEMINARS!!!!!
How to begin?
Deep Dive Into the Literature
You should be reading ……….
Deep Dive Into the Literature
You should be reading on a regular basis!
Deep Dive Into the Literature
You should be reading on a regular basis!
Carefully read one paper every day!!
Deep Dive Into the Literature
Read the primary literature in your area of interest
Start with papers that have high impact/citation numbers
Read “deeply” i.e. the older literature!!
Find the “original” papers in your area of interest.
(these are frequently not the highly cited papers)
Read technique papers!!!!
2) Make a hypothesis
Your hypothesis should be a
quantitative declarative sentence and a
prediction of the experimental results.
Informed by the results of literature
research and perhaps some
preliminary data you or your lab have
gathered
Remember you are testing your
hypothesis: (it could be wrong, either
way you learn from good experiments)
2) Isolate your variable(s)
Good scientific experiments test specific, measurable
parameters called variables.
In general, when you perform an experiment for a range
of values for the variable you are testing
(dose response)
When doing experiments try to adjust only the specific
variable(s) you are testing for
3) Plan you data collection
Know what data you want to
collect and when!
If you do not know the time scale
cover short and
long ranges of time.
Design a table to write down data,
time points, etc.
(Note when things went well and
when “the wheels came off” and
things did not go so well)
4) Be Methodical!!
Carefully plan what reagents you are
going to use and how you will use
them!
Make sure reagents are vetted, fresh,
and at proper temperature and pH.
Keep your experiments to a
schedule!
(do not assume 15 minutes or 30
minutes or 1 day will not matter)
5) Record your data and procedures
Record “all” your data and procedures
immediately into your lab book.
(outliers too)
Repeat experiments, three to five
times. Quantitate your endpoints,
calculate means ± s.d. . Use
appropriate statistical methods to test
for significance.
If appropriate, plot your data (look for
visual trends)
Plot trend lines, best fit curves, etc.
6) Analyze data from each experiment,
organize and state your results,
develop your conclusions.
Was your hypothesis correct?
Were there observable trends in the data?
Were there any unexpected data or trends?
Do you have unanswered questions that can
be the basis of future experiments?
Write up your results into a meeting abstract
and build a poster or talk at a meeting.
Write up a draft of your paper.
Radiation Research
Volume 186, Number 5, November 2016 ISSN 0033–7587
In this issue: Review by Mary Sproull and Kevin Camphausen, “State-of-the-Art Advances in Radiation Biodosimetry for Mass Casualty Events Involving Radiation Exposure”
On the cover:Tilton et al., “Identification of Differential Gene Expression Patterns after Acute Exposure to High and Low Doses of Low-LET Ionizing Radiation in a Reconstituted Human Skin Tissue”
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Submit and publish your best stuff in:
Radiation Research
So…. Why is only 20% of the published
biomedical literature reproducible?
Good Science for “Dummies”
YOU MUST TAKE CONTROL OF YOUR EXPERIMENTS!!
A control is a set of base values against which you use to
compare your experimental results/data from your experiment.
Without these you have no idea whether your results are
higher, lower, or “not statistically different”.
YOU MUST TAKE CONTROL OF YOUR EXPERIMENTS!!
CONTROLS, CONTROLS, CONTROLS…..
A control is a set of base values against which you use to
compare your experimental results/data from your experiment.
Without these you have no idea whether your results are
higher, lower, or “not statistically different”.
YOU MUST TAKE CONTROL OF YOUR EXPERIMENTS!!
Because CONTROLS ARE SO………
Because CONTROLS ARE SO………
BORING!!!!!
To cut or not to cut………
To cut or not to cut………
Quiet, the tumor cells are sleeping…
Quiet, the tumor cells are sleeping…
Quiet, the tumor cells are sleeping…
Quiet, the tumor cells are sleeping…
Quiet, the tumor cells are sleeping…
Quiet, the tumor cells are sleeping…
Quiet, the tumor cells are sleeping…
Quiescent Cells
=
Stem Cells
CONTROLS = SCIENTIFIC RIGOR
CONTROLS = SCIENTIFIC RIGOR
VET YOUR REAGENTS
VET YOUR REAGENTS
VET YOUR REAGENTS
VET YOUR REAGENTS
CONTROLS = SCIENTIFIC RIGOR
ARE YOUR CELLS WHAT YOU
THINK THEY ARE?
ARE YOUR VECTORS, PROBES, & ANTIBODIES
WHAT YOU THINK THEY ARE?
VET YOUR REAGENTS
Deep Dive Into the Literature
Write a NIH Type Specific Aims Page on Your Research Idea