today: inquiry 2 and lab safety

Today: Inquiry 2 and Lab Safety...

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Why use a sample instead of a population?

•Logistics

•Cost

•Time

Samples:

Random- each member of population has an equal chance of being part of the sample.

or

Representative- ensuring that certain parameters of your sample match the population.

Replicates:

Technical vs Experimental

Technical replicate- one treatment is divided into multiple samples.

Experimental replicate- different, replicate, treatments are done to different samples.

Testing blood sugar levels after eating a Snickers:

Testing blood sugar levels after eating a Snickers:

Divide a participants blood into 3 samples and test blood sugar in each sample.

Technical or Experimental replicate?

Testing blood sugar levels after eating a Snickers:

Test 3 different people.

Technical or Experimental replicate?

Testing blood sugar levels after eating a Snickers:

Test the same person on 3 different days.

Technical or Experimental replicate?

What sample size do you need?

What sample size do you need?

It depends on the error you expect.

To determine an appropriate sample size, you need to estimate a few parameters.•Means•Standard Deviation

•Power: The probability that an experiment will have a significant (positive) result, that is have a p-value of less than the specified significance level (usually 5%).

This calculator will help you determine the appropriate sample size:

http://www.stat.ubc.ca/~rollin/stats/ssize/n2.html

What sample size do you need?

It depends on the error you expect.

(So it is impossible to predict with 100% accuracy before the experiment is carried out.)

Inquiry 2 -Who?

•Groups of 3-5 people

Inquiry 2 -Who?

•Groups of 3-5 people

•Divide work

Inquiry 2 -Who?

•Groups of 3-5 people

•Divide work

•Be clear about responsibilities and expectations

•Leave enough time to get everything done without panic

Inquiry 2 -What?

Incredibly, amazingly, fantastically, critically important, essential information has been posted on the class webpage.

•Lab Safety

•Supplies

•Order Forms

•Updated Proposal and Report Formats

Inquiry 2 -What?

•May use gadgets, chemicals, kits, etc

-https://uteach.utexas.edu/direct

-Analytical/physical chemistry storeroom(order form)

-Media kitchen (via chem storeroom)

-Research Streams

CAS registry numbers:

unique identifiers for most chemicalshttp://www.cas.org/expertise/cascontent/registry/regsys.html

Inquiry 2 -Where and When?

You can work in PAI 4.14 during your scheduled lab times, and/or during my other lab times.

M 12-3pm

M 3-6pm

Th 12-3pm

Th 3:30-6pm

Inquiry 2 -Where and When?

You can work during the your scheduled lab times, and/or during my other lab times.

Outside of lab times, you may only work when a TA is present. These times will be posted weekly.

Inquiry 2 -Where?

Lab Safety

Inquiry 2 -Where?

Lab Safety

•Closed toed shoes, sleeves, legs covered to knees, hair out of harms way

Inquiry 2 -Where?

Lab Safety

•Closed toed shoes, sleeves, legs covered to knees, hair out of harms way

•NO FOOD in lab

Inquiry 2 -Where?

Lab Safety

•Closed toed shoes, sleeves, legs covered to knees, hair out of harms way

•NO FOOD in lab

•Be aware of what you are doing ans working with

Inquiry 2 -Where?

Lab Safety

•Closed toed shoes, sleeves, legs covered to knees, hair out of harms way

•NO FOOD in lab

•Be aware of what you are doing ans working with

•Wash hands before leave lab

Inquiry 2 -Where?

Waste Disposal

You are responsible for the safe disposal of all waste you generate.

Inquiry 2 -Where? Waste Disposal

Inquiry 2 -Where?

Waste Disposal

You are responsible for the safe disposal of all waste you generate.

This includes chemical waste and biological waste.

If you make a mistake, spill something, have an accident… tell someone immediately.

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Inquiry 2 -How? Proposal

Mostly the same as inquiry 1

2 additional parts

Inquiry 2 -How? Proposal

Mostly the same as inquiry 1

2 additional parts

In the Experiment section

Include detailed information on equipment and reagents to be used.

Where will you get them

Needed order forms

Inquiry 2 -How? Proposal

Mostly the same as inquiry 1

2 additional parts

Add a Responsibilities sectionDescribing who will do what…

Inquiry 2 -How? Proposal

Proposals will not be approved until sources for all equipment and/or chemicals are secured.

Orders will be placed during lab, after your proposal is approved.

Inquiry 2 -How? Written Reports

Similar to inquiry 1, but add a Responsibilities section

Inquiry 2 -How? Written Reports

Similar to inquiry 1, but add a Responsibilities section

Everyone should participate in writing, some may write the original text, while others work on figures/tables, and others edit.

Inquiry 2 -How? Oral Reports

•Should be 15 to 20 minutes long

Inquiry 2 -How? Oral Reports

•Should be 15 to 20 minutes long

•Include all of the basic information from the written report.(Do not simply read your written report)

Inquiry 2 -How? Oral Reports

•Should be 15 to 20 minutes long

•Include all of the basic information from the written report.(Do not simply read your written report)

•Everyone will present some part of the oral report

Inquiry 2 -How? Oral Reports

•Should be 15 to 20 minutes long

•Include all of the basic information from the written report.(Do not simply read your written report)

•Everyone will present some part of the oral report

•Should be visually engaging

My group sucks, and now I am going to fail.

Don’t have a cow man.Your will be graded as agroup and also only on

your sections.

Inquiry 2 proposals are due in lab on Th 10/1 or M 10/5

•Homework #4 due now:What was the hardest part of inquiry 1, and how will you make this easier for inquiry 2?

This weekend: Pecan Street Arts Festival(http://www.oldpecanstreetfestival.com)

Next week: Research Ethics