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H U M A S P R O G R A M M A N U A L
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HUMAS PROGRAM MANUAL
WATER ANALYZER
Analysis Manual
H U M A S P R O G R A M M A N U A L
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CategoryHUMAS
ProgramMethod
Range
(mg/L)
Program
NO.
Page
NO.
Aluminum HS-Al ECR 0.01~0.3 5000 2
Cadmium HS-Cd Dithizone 0 ~ 0.5 6100 6
Chloride,LR HS-Cl-L Mercuric Thiocyanate 1~20 5100 10
Chloride,HR HS-Cl-H Mercuric Thiocyanate 20~60 5101 13
Chlorine, Free HS-Cl2(Free) DPD 0.1~2 4310 16
Chlorine, Total HS-Cl2(T) DPD 0.1~2 4300 19
Chromium, Hexavalent HS-Cr(+6) Diphenylcarbazide, DPC 0.1~1 4410 22
Chromium, Total HS-Cr(T) Diphenylcarbazide, DPC 0.1~1 4400 25
Cobalt HS-Co PAN 0.05~2 6400 29
COD(Chemical Oxygen Demand),UR COD-UR Reaction Digestion 5~40 1000 33
COD(Chemical Oxygen Demand),LR COD-LR Reaction Digestion 15~150 1001 37
COD(Chemical Oxygen Demand),MR COD-MR, HR Reaction Digestion 50~1500 1002 41
COD(Mn)(Chemical Oxygen Demand),UR COD-Mn-U Reaction Digestion 0.2~3 1502 45
COD(Mn)(Chemical Oxygen Demand),LR COD-Mn-L Reaction Digestion 0.6~20 1500 49
COD(Mn)(Chemical Oxygen Demand),HR COD-Mn-H Reaction Digestion 20~100 1501 53
Color HS-Color Platium Cobalt 50~1000° 6600 57
Copper HS-Cu Bathocuproine 0.1~5 4000 58
Cyanide HS-CN DP 0.005 ~ 0.5 4900 62
Detergents HS-ABS Crystal violet 0.01~0.3 6200 66
Fluoride HS-F SPANDS 0.1~1.5 4700 70
Hardness(Ca)HS-HD(Ca)(Mg) Calmagite 0.1~4
700073
7001Hardness(Mg)
Hydrogen peroxide,LR HS-H2O2 Titanium sulfate 0.5~30 5300 77
Hydrogen peroxide,HR HS-H2O2 Titanium sulfate 3~300 5301 80
Iodine HS-I2 DPD 0.1~2 5200 83
Iron, Ferrous HS-Fe(+2) Phenanthroine 0.1~5 4110 86
Iron, Total HS-Fe(T) Phenanthroine 0.1~5 4100 89
Manganese HS-Mn Periodate Oxidation 0~ 20 4800 92
Nickel HS-Ni PAN 0.05~2 6500 95
Nitrogen, Ammonia,UR HS-NH3(N)-U Cyanuric acid 0.03~1 2800 99
Nitrogen, Ammonia,LR HS-NH3(N)-L Nessler 0.2~6 2810 103
Nitrogen, Ammonia,HR HS-NH3(N)-H Nessler 2~60 2811 107
Nitrogen, Nitrate, HS-NO3(N)-L Cadmium reduction 0.2~5 2200 111
Nitrogen, Nitrate HS-NO3(N)-H Brucine 1~15 2210 115
Nitrogen, Nitrate,CA HS-NO3(N)-CA Chromatropic acid 0.5~30 2220 119
Nitrogen, Nitrite,LR HS-NO2(N)-L NED 0.1~1 2400 122
Nitrogen, Nitrite,HR HS-NO2(N)-H FSS 5~150 2410 126
Nitrogen, Total,UR HS-TN-U Cadmium Reduction 0.2~5 2000 129
Nitrogen, Total,LR HS-TN-L(CA) Chromatropic acid 1~50 2020 134
Nitrogen, Total,HR HS-TN-H(CA) Chromatropic acid 10~100 2021 139
Orthophosphate,LR HS-PO4(P)-L Ascorbic acid 0.01~3 3020 144
Orthophosphate,HR HS-PO4(P)-H Molybdo vanadate 1~15 3030 147
Phenol HS-Phenol Direct Photometric 0.1~10 4500 150
Phosphorus, Total,LR HS-TP-L Ascorbic acid 0.01~3 3000 153
Phosphorus, Total,HR HS-TP-H Molybdo vanadate 1~15 3010 158
Silica-L HS-Si-L Molydosilicate 0.5~20 5500 163
Silica-H HS-SI-H Molybdosilicate 20~100 5510 166
Sulfate,LR HS-SO4-L Gravimetric 5~50 4600 169
Sulfate,HR HS-SO4-H Gravimetric 10~70 4601 172
Sulfide HS-S Methylene blue 0.5~5 5400 175
Turbidity HS-Turb. Absorption 0~400FTU 6700 179
Zinc HS-Zn Zincon 0.1~5 4200 180
■ CATEGORIES
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Aluminium
Method 5000ECR Method
Al(0.01 ~ 0.3mg/L)Scope and Application : water and wastewater
Al (0.01 ~ 0.3mg/L)
1) Add 5ml of distilled water to a sample cell (round glass vial) to prepare reagent blank test.
2) Add 5ml of sample to another sample cell (round glass vial) to be tested using a precise pipet.
3) Add 2ml of S1 to each cell. Cap tightly and shake it up and down slowly for 10 times to mix them.
4) Add one of Powder Pillow(P) to each cell, cap tightly and shake them up and down slowly for 10 times to dissolve the P.
5) Add 1ml of S2 to each cell.
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Aluminium
Aluminium
5) Add five drops of S3 to the only reagent blank cell, contained distilled water. Cap tightly and shake them up and down for 10 times.
6) After 5 minutes, wipe the cell and place it into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.
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Aluminium
Aluminium
InterferencesIf sample to be tested is colorful, other than transparent, it can affect to the actual data.
Floating substance in the sample works as an interference to the test. For the best result, reading of the result should be performed within 5minutes after testing. If fluoride, polyphosphate and alkalinity in the sample, they work as an interference. If there is some sulfate in the sample, the concentration is lower than 2,000 ㎎/L, it does not work as an interference Sample Collection and Preservation
Collect samples using an plastic container and analyze it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated nitric acid to pH of 2~5 (about 1.5㎖ per liter). It can be stored for 6 months at room temperature. Before, analyzing, adjust pH to neutralization with sodium hydroxide solution(5N). In case of that sample contains suspended substances, separate the substance from the samples. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of nitric acid and 4ml of hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 0.08㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Make a standard solution to check accuracy and calibration by commercial standard solution, 1,000mg/L.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.01㎎/L, so test should be performed within appropriate concentration range.
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Aluminium
Item Test No. Cat. No.
HS-Al Set 100/set 27010
Aluminium
Contents
Description Quantity Requiredper test Unit Cat. No.
Al Powder Pillow (P) 1~2 100Ea/set 27010-10
Al Solution - 1 (S1) - 1bottle/set 27010-20
Al Solution - 2 (S2) - 1bottle/set 27010-21
Al Solution - 3 (S3) - 1bottle/set 27010-22
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Aluminium
Cadmium
Method 6000Dithizone
Cd(0.01 ~ 0.5mg/L)Scope and Application : water and wastewater
Cd (0.01 ~ 0.5mg/L)
1) Add 5ml of distilled water to sample cell (round glass vial) to prepare blank test.
2) Add 5ml of sample to another sample cell (round glass vial) to be tested using a precise pipet.
3) Add 1ml of S1 to each sample cell, cap tightly and shake it up and down slowly for 10 times to mix them.
4) Add 0.2ml of S2 to each sample cell, cap tightly and shake it up and down slowly for 10 times to mix them.
5) Add one of powder pillow(P) to each sample cell and shake vigorously until the reagent is completely dissolve.
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Cadmium
Cadmium
6) Wait 2 minutes for maximum color development. At end of 2 minute waiting, clean and insert the vial into cell holder to read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual .
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Cadmium
Cadmium
InterferencesIf sample to be tested is colorful, other than transparent, it can affect to the actual data.
Floating substance in the sample works as an interference to the test. It is necessary to filtering for the best result. A strong oxidizing and reducing agent in the sample will interfere. The substance in the below table lie below the limit at which the determination is interfered with.
Substance Conc.(mg/L) Substance Conc.(mg/L) or %
Aluminum 25 Magnesium 1000
Calcium 1000 Ammonium 1000
Lead 100 Nickel 0.01
Cobalt 1 Nitrite 0.1
Chromium(Cr2O7) 100 Phosphorous 100
Copper 10 Zinc 1
Manganese 50 Na2SO4 1%
NH4+ 100 NaNO3 10%
Iron(3+) 1 NaCl 1%
Sample Collection and PreservationCollect samples using an acid-cleaned glass and plastic container and analyze it
promptly for good result. It can preserve with nitric acid 65%(1ml/1L sample)
Accuracy and PrecisionWhen 0.25㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dilute commercial cadmium standard solution containing 0.1, 0.25 and 0.5 mg/L.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.01㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-Cd Set 25/set 41010
Cadmium
Cadmium
Contents
Description Quantity Requiredper test Unit Cat. No.
Cd Solution(S1) - 1bottle/set 41010-20
Cd Solution(S2) - 1bottle/set 41010-21
Cd Powder Pillow(P) 1~2 25Ea/set 41010-10
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Cadmium
Chloride
Method 5100Mercuric Thiocyanate Method
Cl-L(1 ~ 20mg/L)Scope and Application : water and wastewater
Cl-L (1 ~ 20mg/L) 1) Add 2.5ml of distilled water to sample cell(round glass vial) to prepare reagent blank test.
2) Add 2.5ml of sample to another sample cell(round glass vial) to be tested using a precise pipet.
3) Add 5ml of S-1 to each kit, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix them.
4) Add 0.5ml of S-2 to each kit, which is distilled water and sample add. Cap
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tightly and shake it up and down slowly for 10 times to mix it.
5) Add 1ml of S-3 to each kit, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.
6) Wait 5 minutes for maximum color development. At end of 5 minute waiting, insert the vial into cell holder to read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.
Chloride
Chloride
InterferencesIf sample to be tested is colorful, other than transparent, it can
affect to the actual data. Floating substance in the sample works as an interference to the test. It is necessary to filtering for the best result. A strong oxidizing and reducing agent in the sample will interfere.
Sample Collection and PreservationCollect samples using an acid-cleaned glass and plastic
container and analyze it promptly for good result. It can be stored for 28 days at room temperature.
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
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Chloride
Item Test No. Cat. No.
HS-Cl-L Set 100/set 35012
Standard Solution Dissolve 0.261g of NaCl(Sodium Chloride) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 1㎎/L, so test should be performed within appropriate concentration range.
Chloride
Contents
Description Quantity Requiredper test Unit Cat. No.
Cl-L Solution(S-1) - 1bottle/set 35012-20
Cl-L Solution(S-2) - 1bottle/set 35012-21
Cl-L Solution(S-3) - 1bottle/set 35012-22
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Chloride
Chloride
Method 5101Mercuric Thiocyanate Method
Cl-H(20 ~ 60mg/L)Scope and Application : water and wastewater
Cl-H (20 ~ 60mg/L)
1) Add 2.5ml of distilled water to sample cell(round glass vial) to prepare reagent blank test.
2) Add 2.5ml of sample to another sample cell(round glass vial) to be tested
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using a precise pipet.
3) Add 5ml of S-1 to each kit, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix them.
4) Add 0.5ml of S-2 to each kit, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.
5) Add 1ml of S-3 to each kit, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.
6) Wait 5 minutes for maximum color development. At end of 5 minute waiting, insert the vial into cell holder to read the result. Please refer to chapter 4. HUMAS Program of
Instrument Manual for test procedure.
Chloride
Chloride
InterferencesIf sample to be tested is colorful, other than transparent, it can affect to the actual data.
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Chloride
Floating substance in the sample works as an interference to the test. It is necessary to filtering for the best result. A strong oxidizing and reducing agent in the sample will interfere.
Sample Collection and PreservationCollect samples using an acid-cleaned glass and plastic container and analyze it
promptly for good result. It can be stored for 28 days at room temperature.
Accuracy and PrecisionWhen 30㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.261g of NaCl(Sodium Chloride) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 20㎎/L, so test should be performed within appropriate concentration range.
Chloride
Contents
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Item Test No. Cat. No.
HS-Cl-H Set 100/set 35014
Description Quantity Requiredper test Unit Cat. No.
Cl-H Solution(S-1) - 1bottle/set 35014-20
Cl-H Solution(S-2) - 1bottle/set 35014-21
Cl-H Solution(S-3) - 1bottle/set 35014-22
Chloride
Free Chlorine
Method 4310DPD Method
Cl2(Free)(0.1 ~ 2mg/L)
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Scope and Application : water and wastewater
Cl2(Free) (0.1 ~ 2mg/L)
1) Add 10ml of distilled water to sample cell(round glass vial) to prepare reagent blank test.
2) Add 10ml of sample to another sample cell(round glass vial) to be tested
using a precise pipet.
3) Add one of Powder Pillow(P) to the only sample cell, contained sample to be tested, cap and shake it vigorously to dissolve the reagent for 15 seconds.
4) After 2 minutes, wipe the cells and place it into cell holder and read the result. Please refer to chapter 4.
HUMAS Program of Instrument Manual for test procedure.
Free Chlorine
Free Chlorine
Interferences
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Free Chlorine
1) Oxidizer, Br2, ClO2, I2 and O3 in the sample will interfere to the test.2) High buffered sample is need to adjust pH to 6~7.3) When oxidized manganese(Mn4+, Mn7+) presents in the sample, perform following.
① Adjust pH to between 6~7. ② Add 3 drops of potassium iodide (30g/L). ③ Mix it and leave for one minute. ④ Add 3 drops of sodium arsenite(5/L). ⑤ Test the sample treated by above procedure. ⑥ For the best result, subtract the value measured by above treatment from
the result measured original testing procedure. Sample collection and Preservation
Rinse thoroughly the container(avoid plastic bottle) with deionized or distilled water. Analyze the sample immediately after collection because free chlorine is strong oxidizing agent and it is unstable in natural water. Do not use the same sample for testing of free and total chlorine. When sampling from a tap water, let the water flow for at least 5 minutes to ensure a representative sample. And don't make a bubble in the sample.
Accuracy and PrecisionWhen 1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.891g of KMnO4(Potassium Permanganate) in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎,so test should be performed within appropriate concentration range.
Free Chlorine
Contents
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Item Test No. Cat. No.
HS-Cl2(Free) Set 100/set 10030
Description Quantity Requiredper test Unit Cat. No.
Cl2(Free) Powder Pillow(P) 1~2 100Ea/set 10030-10
Free Chlorine
Chlorine, Total
Method 4300
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DPD MethodCl2(T)(0.1 ~ 2mg/L)
Scope and Application : water and wastewater
Cl2(T) (0.1 ~ 2mg/L)
1) Add 10ml of distilled water to sample cell(round glass vial) to prepare reagent blank test.
2) Add 10ml of sample to another sample cell(round glass vial) to be tested using a precise pipet.
3) Add one of Powder Pillow(P) to the only sample cell,
contained sample to be tested, cap and shake it vigorously to dissolve the reagent for 15 seconds.
4) After 2 minutes, wipe the cells and place it into cell
holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.
Chlorine, Total
Chlorine, Total
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Chlorine, Total
Interferences1) Oxidizer, Br2, ClO2, I2 and O3 in the sample will interfere to the test.2) High buffered sample is need to adjust pH to 6~7.3) When oxidized manganese(Mn4+, Mn7+) presents in the sample, perform following.
① Adjust pH to between 6~7. ② Add 3 drops of potassium iodide (30g/L). ③ Mix it and leave for one minute. ④ Add 3 drops of sodium arsenite(5/L). ⑤ Test the sample treated by above procedure. ⑥ For the best result, subtract the value measured by above treatment from
the result measured original testing procedure. Sample collection and Preservation
Rinse thoroughly the container(avoid plastic bottle) with deionized or distilled water. Analyze the sample immediately after collection because free chlorine is strong oxidizing agent and it is unstable in natural water. Do not use the same sample for testing of free and total chlorine. When sampling from a tap water, let the water flow for at least 5 minutes to ensure a representative sample. And don't make a bubble in the sample.
Accuracy and PrecisionWhen 1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.891g of KMnO4(Potassium Permanganate) in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎,so test should be performed within appropriate concentration range.
Chlorine, Total
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Item Test No. Cat. No.
HS-Cl2(T) Set 100/set 10020
Contents
Description Quantity Requiredper test Unit Cat. No.
Cl2(T) Powder Pillow(P) 1~2 100Ea/set 10020-10
Chlorine, Total
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Chromium, Hexavalent
Method 4410Diphenylcarbazide Method
Cr(+6)(0.1 ~ 1mg/L)Scope and Application : water and wastewater
Cr(+6) (0.1 ~ 1mg/L)
1) Add 10ml of distilled water to sample cell(round glass vial) to prepare reagent blank test.
] 2) Add 10ml of sample to another sample cell(round glass vial) to be tested using a precise pipet.
3) Add Powder Pillow(P) to each cell, cap and shake them intensively for 15 seconds to mix them.
4) After 15 minute reaction, wipe the cells and place them into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test.
Chromium, Hexavalent
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Chromium, Hexavalent
Chromium, Hexavalent
Interferences1) High buffered sample is need to adjust pH to 6~7.2) If samples contain greater than 1mg/L Iron, it may will interfere.
3) If samples contain greater than 1mg/L Vanadium, it may will interfere.
Sample Collection and Preservation Collect samples using an acid-cleaned glass and plastic container and analyze it promptly for good result. It can be stored for 24 Hrs at 4℃ and it should be analyzed within 24 Hrs.
Accuracy and PrecisionWhen 0.5㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.283g of K2Cr2O7(Potassium Dichromate) which has been dried for more than 3 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution. Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-Cr(+6) Set 100/set 11010
Chromium, Hexavalent
Contents
Description Quantity Requiredper test Unit Cat. No.
Cr(+6) Powder Pillow(P) 1~2 100/set 11010-10
Chromium, Hexavalent
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Chromium, Total
Method 4400Diphenylcarbazide Method
Cr(T)(0.1 ~ 1mg/L)Scope and Application : water and wastewater
Cr(T) (0.1 ~ 1mg/L)
1) Add 10ml of distilled water to sample cell(round glass vial) to prepare reagent blank test.
2) Add 10ml of sample to another sample cell(round glass vial) to be tested using a precise pipet.
3) Add one of Powder Pillow-1(P1) to two sample cells respectively.
4) Add one of Powder Pillow-2(P2) to each sample cell, and cap the vials tightly and shake the cells vigorously for 30 seconds to mix them.
5) Place the vials in the reactor(HS-R 200) for 25 minutes at 100℃. (Make the reactor's temperature to 100℃ in advance)
6) After times over, cool the sample cells to room temperature with cold water or tap water.
7) Add one of Powder Pillow-3(P3) to each cell, and shake the vials up and down intensively for 30 seconds to mix them.
8) Add one of Powder Pillow-4(P4) to each cell, and shake the vials up and down intensively for 30 seconds to mix them. 9) Add one of Powder Pillow-5(P5) to each cell, and shake
the vials up and down intensively for 30 seconds to mix them.
Chromium, Total
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Chromium, Total
10) Wait 15 minutes for reaction.
11) Wipe the cells and place vials into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual.
Chromium, Total
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Chromium, Total
Chromium, Total
Interferences1) High buffered sample is need to adjust pH to 6~7.2) Large amount of organic materials in the samples will interfere to completed oxidize
Chromium(3+). Therefore, it should be digested before testing for the best result.
Sample Collection and Preservation Collect samples using an acid-cleaned glass and plastic container and analyze it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated nitric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 6 months at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N). ※ Compensation as adding the reagent Ex.) When sample which has 2ml of nitric acid sodium hydroxide add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 0.5㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.283g of K2Cr2O7(Potassium Dichromate) which has been dried for more than 3 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution. Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-Cr(T) Set 100/set 12010
Chromium, Total
Contents
Description Quantity Requiredper test Unit Cat. No.
Cr(T) Powder Pillow-1(P1) 1~2 100Ea/set 12010-10
Cr(T) Powder Pillow-2(P2) 1~2 100Ea/set 12010-11
Cr(T) Powder Pillow-3(P3) 1~2 100Ea/set 12010-12
Cr(T) Powder Pillow-4(P4) 1~2 100Ea/set 12010-13
Cr(T) Powder Pillow-5(P5) 1~2 100Ea/set 12010-14
Chromium, Total
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Cobalt
Method 6400PAN Method
Co(0.05 ~ 2mg/L)Scope and Application : water and wastewater
Cobalt (0.05 ~ 2mg/L)
1) Add 10ml of distilled water to a sample cell(round glass vial) to prepare reagent blank test.
2) Add 10ml of sample to another sample cell(round glass vial) to be tested using a precise pipet.
3) Add one of Powder Pillow-1(P1) to each cell, which is distilled water and sample add. Cap tightly and shake it vigorously to dissolve it.
4) Add 0.5ml of S to each cell. Cap tightly and shake it up and down slowly for 10 times to mix it.
5) Wait 15minutes for reaction.
Cobalt
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Cobalt
6) Add one of Powder Pillow-2(P2) to each cell, which is distilled water and sample add. Cap tightly and shake It gently to dissolve it.
7) After 1minute reaction, wipe the cells and place vials into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual.
Cobalt
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Cobalt
Interferences
Substance Conc.(mg/L) Substance Conc.(mg/L) or %
Al 60 K 500
Ca 1000(as CaCO3) Mg 400
Cd 30 Mn 45
Cl 8000 Mo 60
Complex reagents All range Na 5000
Cr(+3) 20 Pb 30
Cr(+6) 80 Zn 40
Cu 15 Buffer reagents Pre-treatment
F(-) 20
Ni All rangeFe(+2) All range
Fe(+3) 30
Sample Collection and Preservation
Take samples using a plastic container cleaned with acids and analyze it promptly for good result. If prompt analysis is not possible, add 5㎖ of concentrated nitric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 6 months at room temperature. Before analyzing, adjust pH of sample to neutral range. When pH of sample is higher than 8, sediments will be produced in the sample which work as a interfere. ※ Compensation as adding the reagent Ex.) When sample which has 5ml of nitric acid and sodium hydroxide add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1000㎖ + 5㎖ + 4 = 1009㎖○ Calculating compensation value = 1009/1000 =1.009○ Actual Concentration = 10㎎/L × 1.009 = 10.09㎎/L
Accuracy and PrecisionWhen 1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionA commercial standard solution(1000㎖/l) is recommend to dilute proper
concentration.
Detection LimitErrors are more frequent if the concentration of a sample is lower than 0.05㎎/L.
Cobalt
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Item Test No. Cat. No.
HS-Co Set 100/set 43010
Cobalt
Contents
Description Quantity Requiredper test Unit Cat. No.
Co Powder Pillow-1(P1) 1~2 100Ea/set 43010-10
Co Powder Pillow-2(P2) 1~2 100Ea/set 43010-11
Co Solution(S) - 1bottle/set 43010-20
Cobalt
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Chemical Oxygen Demand(CODCr)
Method 1000Reactor Digestion Method
COD-U(5 ~ 40mg/L)Scope and Application : water and wastewater
COD-UR (5 ~ 40mg/L)
1) To prepare the reagent blank test, add 2㎖ of distilled water to COD-U Kit.
2) Take 2㎖ of a sample to be tested using a precise pipet and add to different COD-U Kit.
3) Close it with cap and shake it up and down slowly for 10 times to mix it.
4) Put the kits to reactor(HS-R-200) and heat it for 2 hours at 150℃ for pre-treatment. Place the plastic cover on the reactor. (Make the reactor's temperature to 150℃ in advance)
Chemical Oxygen Demand(CODCr)
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Chemical Oxygen Demand(CODCr)
Chemical Oxygen Demand(CODCr)
5) After heating reaction, take the kits from the reactor and leave it on for 10 minutes at room temperature. Then, shake it up and down softly for 10 times to mix it. And cool the vials to room temperature, clean outside of the vials.
6) See Chapter 4. HUMAS Program of the Instrument Manual.
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Chemical Oxygen Demand(CODCr)
Chemical Oxygen Demand(CODCr)
InterferencesIf there is some chloride(Cl-) in the sample, the measured concentration can be higher
than the actual concentration. The concentration is lower than 2,500㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride.
Sample Collection and PreservationTake samples using a glass container and analyze it promptly for good result. If
prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 30㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1000㎖ + 2㎖ = 1002㎖○ Calculating compensation value = 1002/1000 =1.002○ Actual Concentration = 30㎎/L × 1.002 = 30.06㎎/L
Accuracy and PrecisionWhen 30㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionGrind Potassium hydrogen phthalate(KHP) in fine size and dry it for 2 hours at 110℃.
Then, the dried KHP of 0.425g is dissolved in distilled water of 1000㎖. It becomes standard solution of COD 500㎎/L and refrigerated at 4℃.
Detection LimitErrors are more frequent if the concentration of a sample is lower than 5㎎/L.
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Item Test No. Cat. No.
HS-COD-UR Set 25/set 01011
Chemical Oxygen Demand(CODCr)
Contents
Description Quantity Requiredper test Unit Cat. No.
COD-UR Kit 1~2 25Ea/box 01011-01
Chemical Oxygen Demand(CODCr)
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Chemical Oxygen Demand(CODCr)
Method 1001Reactor Digestion Method
COD-L(15 ~ 150mg/L)Scope and Application : water and wastewater
COD-L (15 ~ 150mg/L)
1) To prepare the reagent blank test, add 2㎖ of distilled water to COD-L Kit.
2) Take 2㎖ of a sample to be tested using a precise pipet and add to different COD-L Kit.
3) Close it with cap and shake it up and down slowly for 10 times to mix it.
4) Put the kits to reactor(HS-R 200) and heat it for 2 hours at 150℃ for pre-treatment. Place the plastic cover on the reactor. (Make the reactor's temperature to 150℃ in advance)
Chemical Oxygen Demand(CODCr)
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Chemical Oxygen Demand(CODCr)
Chemical Oxygen Demand(CODCr)
5) After heating reaction, take the kits from the reactor and leave it on for 10 minutes at room temperature. Then, shake it up and down softly for 10 times to mix it. And cool the vials to room temperature, clean outside of the vials.
6) See Chapter 4. HUMAS Program of the Instrument Manual and measure the sample.
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Chemical Oxygen Demand(CODCr)
Chemical Oxygen Demand(CODCr)
InterferencesIf there is some chloride(Cl-) in the sample, the measured concentration can be higher
than the actual concentration. The concentration is lower than 3,000㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride.
Sample Collection and PreservationTake samples using a glass container and analyze it promptly for good result. If
prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 100㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1000㎖ + 2㎖ = 1002㎖○ Calculating compensation value = 1002/1000 =1.002○ Actual Concentration = 100㎎/L × 1.002 = 100.2㎎/L
Accuracy and PrecisionWhen 100㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionGrind Potassium hydrogen phthalate(KHP) in fine size and dry it for 2 hours at 110℃.
Then, the dried KHP of 0.425g is dissolved in distilled water of 1000㎖. It becomes standard solution of COD 500㎎/L and refrigerated at 4℃.
Detection LimitErrors are more frequent if the concentration of a sample is lower than 15㎎/L.
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Item Test No. Cat. No.
HS-COD-LR Set 25/set 01012
Chemical Oxygen Demand(CODCr)
Contents
Description Quantity Requiredper test Unit Cat. No.
COD-LR Kit 1~2 25Ea/set 01012-01
Chemical Oxygen Demand(CODCr)
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Chemical Oxygen Demand(CODCr)
Method 1002Reactor Digestion Method
COD-M(50 ~ 1,500mg/L), COD-H(500~15,000mg/L)Scope and Application : water and wastewater
COD-M (50 ~ 1,500mg/L), COD-H (500 ~ 15,000mg/L)
1) To prepare the reagent blank test, add 2㎖ of distilled water to COD-M Kit. (In case of COD-H, add 0.2mL).
2) Take 2㎖ of a sample to be tested using a precise pipet and add to different COD-M Kit. (In case of COD-H, add 0.2mL).
3) Close it with cap and shake it up and down slowly for 10 times to mix it.
4) Put the kits to reactor(HS-R 200) and heat it for 2 hours at 150℃ for pre-treatment. Place the plastic cover on the reactor. (Make the reactor's temperature to 150℃ in advance)
Chemical Oxygen Demand(CODCr)
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Chemical Oxygen Demand(CODCr)
Chemical Oxygen Demand(CODCr)
5) After heating reaction, take the kits from the reactor and leave it on for 10 minutes at room temperature. Then, shake it up and down softly for 10 times to mix it. And cool the vials to room temperature, clean outside of the vials.
6) See Chapter 4. HUMAS Program of the Instrument Manual and measure the samples. ※When using COD-H Kit, measure the sample by COD-M Program and multiply the value by 10.
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Chemical Oxygen Demand(CODCr)
Chemical Oxygen Demand(CODCr)
InterferencesIf there is some chloride(Cl-) in the sample, the measured concentration can be higher
than the actual concentration. In case of COD-M, Cl- is under 3,000㎎/L, it does not work as an interference. n case of COD-H, Cl- is under 40,000㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride.
Sample Collection and PreservationTake samples using a glass container and analyze it promptly for good result. If
prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 1000㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ = 1,002㎖○ Calculating compensation value = 1,002/1,000 =1.002○ Actual Concentration = 1,000㎎/L × 1.002 = 1,002㎎/L
Accuracy and PrecisionWhen 500㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionGrind Potassium hydrogen phthalate(KHP) in fine size and dry it for 2 hours. Then,
the dried KHP of 0.425g is dissolved in distilled water of 1000㎖. It becomes standard solution of COD 500㎎/L and refrigerated at 4℃.
Detection LimitCOO-M : Errors are more frequent if the concentration of a sample is lower
than 50㎎/L.COD-H : Errors are more frequent if the concentration of a sample is lower
than 500㎎/L.
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Item Test No. Cat. No.
HS-COD-M Set 25/set 01013
HS-COD-H Set 25/set 01014
Chemical Oxygen Demand(CODCr)
Contents
Description Quantity Requiredper test Unit Cat. No.
COD-M Kit (50 ~ 1,500mg/L) 1~2 25Ea/set 01013-01
COD-H Kit (500 ~ 15,000mg/L) 1~2 25Ea/set 01014-01
Chemical Oxygen Demand(CODCr)
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Chemical Oxygen Demand(CODMn)
Method 1502Reactor Digestion Method
COD(Mn)-L(0.2 ~ 3mg/L)Scope and Application : water and wastewater
COD(Mn)-U (0.2 ~ 3mg/L)
1) To prepare the reagent blank test, add 5㎖ of distilled water to COD-Mn-U Kit.
2) Take 5㎖ of sample to be tested using a precise pipette and add to different COD-Mn-U Kit.
3) Add 0.6ml of S to each COD-Mn-U Kit(vial) using a precise pipet.
4) Close it with cap and shake it up and down slowly for 10 times to mix it.
Chemical Oxygen Demand(CODMn)
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Chemical Oxygen Demand(CODMn)
Chemical Oxygen Demand(CODMn)
5) Put the kit to reactor(HS-R 200) and heat it for 15 minutes at 100℃. Place the plastic cover on the reactor. (Make the reactor's temperature to 100℃ in advance)
6) After heating reaction, take the kits from the reactor and cool it down to room temperature using cold water such as tap water. And clean outside of the vials. (After cooling, it should be measured within 3 minutes. It is very important to the best results)
7) See Chapter 4. HUMAS Program of the Instrument Manual and measure the samples.
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Chemical Oxygen Demand(CODMn)
Chemical Oxygen Demand(CODMn)
Interferences
Substance Conc.(mg/L)
Sulfide All range
Calcium 100
Magnesium 200
Free chlorine 3.5
Other oxidants Ozone, Hydrogen peroxide
Sample Collection and PreservationCollect samples using a glass container and analyze it promptly for good result. If
prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ = 1,002㎖○ Calculating compensation value = 1,002/1,000 =1.002○ Correct Concentration = 10㎎/L × 1.002 = 10.02㎎/L
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from
the actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionGlucose(C6H12O6) in fine size and dry it for 1 hour. After cooling it to room
temperature in desiccator, the dried Glucose of 0.938g is dissolved in distilled water of 1000㎖. It becomes standard solution of COD 567㎎/L and refrigerated at 4℃.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.6㎎/L. So, test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-COD-Mn-U Set 25/set 01120
Chemical Oxygen Demand(CODMn)
Chemical Oxygen Demand(CODMn)
Contents
Description Quantity Requiredper test Unit Cat. No.
COD(Mn)-U Kit 1~2 25Ea/set 01120-01
COD(Mn)-U Solution(S) - 1bottle/set 01120-20
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Chemical Oxygen Demand(CODMn)
Method 1500Reactor Digestion MethodCOD(Mn)-L(0.6 ~ 20mg/L)
Scope and Application : water and wastewater
COD(Mn)-L (0.6 ~ 20mg/L)
1) To prepare the reagent blank test, add 3㎖ of distilled water to COD-Mn-L Kit.
2) Take 3㎖ of sample to be tested using a precise pipette and add to different COD-Mn-L Kit.
3) Add 0.6ml of S to each COD-Mn-L Kit(vial) using a precise pipet.
4) Close it with cap and shake it up and down slowly for 10 times to mix it.
Chemical Oxygen Demand(CODMn)
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Chemical Oxygen Demand(CODMn)
Chemical Oxygen Demand(CODMn)
5) Put the kit to reactor(HS-R 200) and heat it for 15 minutes at 100℃. Place the plastic cover on the reactor. (Make the reactor's temperature to 100℃ in advance)
6) After heating reaction, take the kits from the reactor and cool it down to room temperature using cold water such as tap water. And clean outside of the vials. (After cooling, it should be measured within 3 minutes. It is very important to the best results)
7) See Chapter 4. HUMAS Program of the Instrument Manual and measure the samples.
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Chemical Oxygen Demand(CODMn)
Chemical Oxygen Demand(CODMn)
InterferencesIf there is some chloride(Cl-) in the sample, the measured concentration can be higher
than the actual concentration. The concentration is lower than 200㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride.
Sample Collection and PreservationCollect samples using a glass container and analyze it promptly for good result. If
prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ = 1,002㎖○ Calculating compensation value = 1,002/1,000 =1.002○ Correct Concentration = 10㎎/L × 1.002 = 10.02㎎/L
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from
the actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionGlucose(C6H12O6) in fine size and dry it for 1 hour. After cooling it to room
temperature in desiccator, the dried Glucose of 0.938g is dissolved in distilled water of 1000㎖. It becomes standard solution of COD 567㎎/L and refrigerated at 4℃.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.6㎎/L. So, test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-COD-Mn-L Set 25/set 01110
Chemical Oxygen Demand(CODMn)
Contents
Description Quantity Requiredper test Unit Cat. No.
COD(Mn)-L Kit 1~2 25Ea/set 01110-01
COD(Mn)-L Solution(S) - 1Bottle/set 01110-20
Chemical Oxygen Demand(CODMn)
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Chemical Oxygen Demand(CODMn)
Method 1501Reactor Digestion Method
COD(Mn)-H(20 ~ 100mg/L)Scope and Application : water and wastewater
COD(Mn)-H (20 ~ 100mg/L)
1) To prepare the reagent blank test, add 0.5㎖ of distilled water to COD-Mn-H Kit.
2) Take 0.5㎖ of a sample to be tested using a precise pipet and add to different COD-Mn-H Kit.
3) Add 0.6ml of S to each COD-Mn-H Kit(vial) using a precise pipet.
4) Close it with cap and shake it up and down slowly for 10 imes to mix it.
Chemical Oxygen Demand(CODMn)
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Chemical Oxygen Demand(CODMn)
Chemical Oxygen Demand(CODMn)
5) Put the kit to reactor(HS-R 200) and heat it for 15 minutes at 100℃. Place the plastic cover on the reactor. (Make the reactor's temperature to 100℃ in advance)
6) After heating reaction, take the kits from the reactor and cool it down to room temperature using cold water such as tap water. And clean outside of the vials. (After cooling, it should be measured within 3 minutes. It is very important to the best results)
7) See Chapter 4. HUMAS Program of the Instrument Manual.
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Chemical Oxygen Demand(CODMn)
Chemical Oxygen Demand(CODMn)
InterferencesIf there is some chloride(Cl-) in the sample, the measured concentration can be higher
than the actual concentration. The concentration is lower than 600㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride.
Sample Collection and PreservationCollect samples using a glass container and analyze it promptly for good result. If
prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ = 1,002㎖○ Calculating compensation value = 1,002/1,000 =1.002○ Correct Concentration = 10㎎/L × 1.002 = 10.02㎎/L
Accuracy and PrecisionWhen 50㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionGlucose(C6H12O6) in fine size and dry it for 1 hour. After cooling it to room
temperature in desiccator, the dried Glucose of 0.938g is dissolved in distilled water of 1000㎖. It becomes standard solution of COD 567㎎/L and refrigerated at 4℃.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.6㎎/L. So, test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-COD-Mn-H Set 25/set 19014
Chemical Oxygen Demand(CODMn)
Contents
Description Quantity Requiredper test Unit Cat. No.
COD(Mn)-H Kit 1~2 25Ea/set 19014-01
COD(Mn)-H Solution(S) - 1bottle/set 19014-20
Chemical Oxygen Demand(CODMn)
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Color
Method No. 6600Platinum Cobalt
Color (50~1000°)Scope and Application : water and wastewater
Color (50 ~ 1000°)
1) Add 5ml of distilled water to sample cell(round glass vial) to prepare reagent blank test.
2) Add 5ml of sample to another sample cell(round glass vial) to be tested using a precise pipet.
3) Wipe place the vials into the cell holder to read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.
Color
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Copper
Method 4000Bathocuproine Method
Cu(0.1 ~ 5mg/L)Scope and Application : water and wastewater
Cu (0.1 ~ 5mg/L)
1) Add 5ml of distilled water to sample cell(round glass vial) to prepare reagent blank test.
2) Add 5ml of sample to another sample cell(round glass vial) to be tested using a precise pipet.
3) Add 0.3ml of S-1 to each sample cell, which is distilled water and sample add.Cap tightly and shake the vials up and down for 10 times to mix them.
4) Add 1ml of S-2 to each sample cell, which is distilled water and sample add. Cap tightly and shake the vials up and down for 10 times to mix them
5) Add 0.5ml of S-3 to each sample cell, which is distilled water and sample contained.
Copper
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Copper
5) Cap the vials and gently invert the vials for 10 times.
6) After 10 minute reaction, wipe place the vials into the cell holder to read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual .
Copper
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Copper
InterferencesThe following substances have been tested for interference and showed not work as
interference up to indicated levels.
Substance Conc.(mg/L) Substance Conc.(mg/L)
Al 100 Na 1000
Be 10 Zn 200
Cd 100 Cl- 1000
Ca 1000 F- 500
Cr(+6) 10 NO3 200
Cr(+3) 5 NO2 200
Iron(+2) 100 PO4 1000
Iron(+3) 100 SO4 1000
Li 500 Cl2 1
Ni 100 pH 4 ~ 7
Sample Collection and PreservationCollect samples using a glass and plastic container and analyze it promptly for good
result. If prompt analysis is not possible, add 2㎖ of concentrated nitric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N). In case of that pH of sample is higher than 6, copper will precipitate.※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L Accuracy and Precision
When 1㎎/L of standard solution is investigated, check it the difference from the actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.393g of CuSO4․5H2O(Cuperic Sulfate Pentahydrate) in 20ml of nitric acid(1+1), and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.
Copper
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Copper
Item Test No. Cat. No.
HS-Cu Set 100/set 15010
Copper
Contents
Description Quantity Requiredper test Unit Cat. No.
Cu Solution-1 (S1) - 1bottle/set 15010-20
Cu Solution-2 (S2) - 1bottle/set 15010-21
Cu Solution-3 (S3) - 1bottle/set 15010-22
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Cyanide
Method 4900DP Method
CN(0.005 ~ 0.5mg/L)Scope and Application : water and wastewater
CN (0.005 ~ 0.5mg/L)
1) Add 10ml of distilled water to CN Kit to prepare reagent blank test.
2) Add 10ml of sample to CN Kit to be tested using a precise pipet.
3) Add one of Powder Pillow-1(P1) to each kit, which is distilled water and sample add. Cap tightly and shake them vigorously to dissolve the reagent.
4) Place the vials in the reactor(HS-R 200) for 30 minutes at 120℃. (Make the reactor's temperature to 120℃ in advance).
Place the plastic cover on the reactor. After heating reaction, remove the kits from the reactor and cool it to room temperature.
Cyanide
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Cyanide
Cyanide
5) Add 4 drops of S to each vial, and shake them up and down slowly for 10 times to mix them.
6) Add one of Powder Pillow-2(P2) to each kit, which is distilled water and sample add. Cap tightly and shake them vigorously to dissolve the reagent.
7) Add one of Powder Pillow-3(P3) to each kit, which is distilled water and sample add. Cap tightly and shake them vigorously to mix them.
8) Take 10 minute reaction, wipe kits and place it into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.
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Cyanide
Cyanide
Interferences
Substance Conc.(mg/L) Substance Conc.(mg/L) or %
Silver 10 Mercury 0.1
Aluminum 100 Magnesium 1000
Bromine 0.1 Manganese 1000
Calcium 1000 Ammonium 1000
Cadmium 100 Nickel 0.01
Cobalt 1 Nitrite 10
Chromium(3+) 100 Phosphorous 1000
Copper 5 Sulfate 5
Fluorine 1000 Zinc 100
Iron(2+) 50 EDTA 1%
Iron(3+) 100 NaCl 10%
Sample Collection and Preservation
Collect samples using an acid-cleaned glass and plastic container and analyze it promptly for good result. If prompt analysis is not possible, add 4㎖ of sodium hydroxide solution(5N) to pH of higher than 12. It can be stored for 14days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with acid solution. In case of that sample contains sulfate, test the sample within 24 hours. ※ Compensation as adding the reagent Ex.) When sample which has 4ml of sodium hydroxide solution and 2ml of acid solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 0.1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 2.51g of KCN(Potassium Cyanide) in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.005㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-CN Set 25/set 25010
Cyanide
Contents
Description Quantity Requiredper test Unit Cat. No.
CN Kit 1~2 25Ea/set 25010-01
CN Powder Pillow-1(P1) 1~2 25Ea/set 25010-10
CN Powder Pillow-2(P2) 1~2 25Ea/set 25010-11
CN Powder Pillow-3(P3) 1~2 25Ea/set 25010-12
CN Solution(S) - 1bottle/set 25010-20
Cyanide
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Detergent
Method 6200Crystal violet MethodABS(0.01 ~ 0.3mg/L)
Scope and Application : water and wastewater
ABS (0.01 ~ 0.3mg/L)
1) Fill a clean 250ml separatory funnel to the 200ml mark with sample.
2) Add 5ml of S-1 to the separatory funnel to using a precise pipet. And shake slowly for 5 seconds. Be careful not over flow, it can effect the result. Harmful to skin and cloth.
3) Add one of Powder Pillow(P) to the funnel, and cap tightly and shake slowly to dissolve the reagent completely.
4) Add 20ml of S-2 to the separatory funnel to using a precise pipet. And shake slowly for 1 minutes. And wait 30 minutes for the reaction.
Detergent
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Detergent
5) And wait 30 minutes for the reaction.
6) After 30 minutes, drain the bottom layer of the funnel. It should be treated by regulated method.
7) Drain the top layer of the funnel to a sample cell, and cap and wipe for measuring. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure. To prepare blank cell, add 5㎖ of concentrated benzene to another sample cell.
Detergent
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Detergent
Interferences A large amount chloride will interfere to the test.
Sample Collection and PreservationCollect samples using an plastic or glass container and analyze it promptly for good
result. If prompt analysis is not possible, it can be stored for 24 hours at 4℃. Before, analyzing, warm sample to room temperature.
Accuracy and PrecisionWhen 0.1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean. Standard Solution To check accuracy of this instrument, use a commercial standard solution. And dilute the standard solution within proper concentration. Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.001㎎/L, so test should be performed within appropriate concentration range.
Detergent
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Item Test No. Cat. No.
HS-ABS Set 100/set 40010
Detergent
Contents
Description Quantity Requiredper test Unit Cat. No.
ABS Solution(S-1) - 1bottle/set 40010-20
ABS Solution(S-2) - 1bottle/set 40010-21
ABS Powder Pillow-1(P1) 1~2 100Ea/set 40010-10
Detergent
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Fluoride
Method 4700SPANDS Method
F-(0.1 ~ 1.5mg/L)Scope and Application : water and wastewater
F- (0.1 ~ 1.5mg/L)
1) Add 5ml of distilled water to a sample cell(round glass vial) to prepare reagent blank test.
2) Add 5ml of sample to another sample cell(round glass vial) to be tested using a precise pipet.
3) Add 1ml of S to each cell, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix them.
4) After 1 minute reaction, wipe the cells and place it into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual.
Fluoride
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Fluoride
Fluoride
Interferences
Substance Conc. (mg/L)
Alkalinity 5000mg/L(as CaCO3)
Aluminum 0.1mg/L
Chloride 7000mg/L(as Cl-)
Chorine 5mg/L(as Cl2)
Ferrous 10mg/L(as Iron, ferric)
Orthophosphate 200mg/L(as PO4-3)
Sample Collection and Preservation
Collect samples using an acid-cleaned plastic container and analyze it promptly for good result. If prompt analysis is not possible, it can be stored for 7days at 4℃. Before, analyzing, warm sample to room temperature.
Accuracy and PrecisionWhen 1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean. Standard Solution Dissolve 2.21g of NaF(Sodium Fluoride) which has been heated for 50 minutes at 550 ℃ and cooled in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution. Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-F- Set 100/set 26010
Fluoride
Contents
Description Quantity Requiredper test Unit Cat. No.
F- Solution(S) - 1bottle/box 26010-10
Fluoride
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Hardness
Hardness
Method No. 7000(Ca Hardness), 7001(Mg Hardness)Calmagite Method
Hardness(0.1 ~ 4mg/L)Scope and Application : water and wastewater
Hardness (0.1 ~ 4mg/L)
1) Pour the 100ml sample which should be diluted as the
following example, into graduated cylinder.
※ Example of dilution
① Soften water (0~75 mg/L) : Dilute20 times with distilled water
② Light soften water (75~150 mg/L) : Dilute 50 times with distilled water ③ Hard water (150~350 mg/L) : Dilute 100 times with distilled water
2) Add 1ml of S-1 to the sample. Cap tightly and shake it
gently for 10 times.
3) Add 2ml of S-2 to the sample. Cap tightly and shake it
gently to mix well for 10 times.
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Hardness
Hardness(Mg+2&Ca+2)
4) Pour 10 ml of the sample added reagents into each of
three sample cells. (First, Second and Third)
5) Add one drop of S-3 to the First cell, and shake gently up
and down for 10 times. (For the blank test)
6) Add one drop of S4 to Second cell, and shake up and
down for 10 times to mix it.
7) Select category Mg+2(HD). To set zero base, insert the First
cell added S3 into cell holder and press zero button.
8) Place the Second cell added S3 into cell holder, and read the result of Mg+2(HD).
(Record the data)
9) After measurement of Mg+2(HD), don't remove the Second cell to set zero base.
Select category Ca+2(HD) and do zero base.
10) Place the Third cell into cell holder, and read the result of Ca+2(HD). (Record the
data)
11) Total Hardness = Mg+2(HD) + Ca+2(HD)
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Hardness
Hardness(Mg+2&Ca+2)
Interferences
Substance Conc. (mg/L)
Chromium(+3) 0.25mg/L
Copper 0.75mg/L
EDTA, EGTA 0.2mg/L(as CaCO3-)
Iron(+2) 1.4mg/L
Iron(+3) 2.0mg/L
Manganese(+2) 0.2mg/L
Zinc(+2) 0.05
Calcium>1.0 mg/LMagnesium>0.25 mg/L
For the best result, if Calcium is over 1.0 and Magnesium is over 0.25mg./L, re-test the sample.
Sample Collection and Preservation
Collect samples using an acid-cleaned plastic container and analyze it promptly for good result. If prompt analysis is not possible, it can be stored for 7days at 4℃ after adding 5ml nitric acid to adjust pH 2. Before, analyzing, warm sample to room temperature.
Accuracy and PrecisionWhen 0.5㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean. Standard Solution Dissolve 2.775g of MgCl2(Sodium Magnesium) in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution. Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
Hardness Set 100/set 24020
Hardness
Hardness(Mg+2&Ca+2)
Contents
Description Quantity Requiredper test Unit Cat. No.
Hardness S1 - 1bottle/set 24020-20
Hardness S2 - 1bottle/set 24020-21
Hardness S3 - 1bottle/set 24020-22
Hardness S4 - 1bottle/set 24020-23
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Hydrogen peroxide
Method 5300
Titanium sulfate Method
H2O2-L(0.5 ~ 30mg/L)
Scope and Application : water and wastewater
H2O2-L (0.5 ~ 30mg/L)
1) Add 5ml of distilled water to sample cell(round glass vial)
to prepare reagent blank test.
2) Add 5ml of sample to another sample cell(round glass
vial) to be tested using a precise pipet.
3) Add 0.5ml of S-1 to each cell, which is distilled water
and sample add. Cap tightly and shake it up and down
slowly for 10 times to mix it.
4) Add 1ml of S-2 to each cell, which is distilled water and
sample add. Cap tightly and shake it up and down slowly
for 10 times to mix it.
5) Wait 1 minutes for maximum color development. At end
of 2 minute waiting, insert the vial into cell holder to read
the result. Please refer to chapter 4. HUMAS Program of
Instrument Manual for test procedure.
Hydrogen peroxide
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Hydrogen peroxide
Hydrogen peroxide
Interferences If sample to be tested is colorful, other than transparent, it can affect to the actual data.
Floating substance in the sample works as an interference to the test.
Sample Collection and Preservation Collect samples using an acid-cleaned glass and plastic container and analyze it
promptly for good result.
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more
than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionTo make standard solution, dilute to 1,000mg/L using a commercial Hydrogen
peroxide.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.5㎎/L, so
test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
H2O2-L Set 100/set 24020
Hydrogen peroxide
Contents
DescriptionQuantity Required
per test(vials)Unit Cat. No.
H2O2-L Solution(S-1) - 1bottle/set 26012-20
H2O2-L Solution(S-2) - 1bottle/set 26012-21
Hydrogen peroxide
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Hydrogen peroxide
Method 5301
Titanium sulfate Method
H2O2-H(3 ~ 300mg/L)
Scope and Application : water and wastewater
H2O2-H (3 ~ 300mg/L)
1) Add 1ml of distilled water to sample cell(round glass vial)
to prepare reagent blank test.
2) Add 1ml of sample to another sample cell(round glass
vial) to be tested using a precise pipet.
3) Add 4ml of S-1 to each cell, which is distilled water and
sample add. Cap tightly and shake it up and down slowly
for 10 times to mix it.
4) Add 1ml of S-2 to each cell, which is distilled water and
sample add. Cap tightly and shake it up and down slowly
for 10 times to mix it.
5) Wait 1 minutes for maximum color development. At end
of 2 minute waiting, insert the vial into cell holder to read
the result. Please refer to chapter 4. HUMAS Program of
Instrument Manual for test procedure.
Hydrogen peroxide
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Hydrogen peroxide
Hydrogen peroxide
Interferences If sample to be tested is colorful, other than transparent, it can affect to the actual data.
Floating substance in the sample works as an interference to the test.
Sample Collection and PreservationCollect samples using an acid-cleaned glass and plastic container and analyze it
promptly for good result.
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more
than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionTo make standard solution, dilute to 1,000mg/L using a commercial Hydrogen
peroxide.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 3㎎/L, so
test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
H2O2-H Set 100/set 26014
Hydrogen peroxide
Hydrogen peroxide
Contents
DescriptionQuantity Required
per test(vials)Unit Cat. No.
H2O2-H Solution(S-1) - 1bottle/set 26014-20
H2O2-H Solution(S-1) - 1bottle/set 26014-21
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Iodine
Method 5200DPD Method
I2(0.1 ~ 2mg/L)
Scope and Application : water and wastewater
l2 (0.1 ~ 2mg/L)
1) Add 10ml of distilled water to sample cell (round glass
vial) to prepare reagent blank test.
2) Add 10ml of sample to another sample cell (round glass
vial) to be tested using a precise pipet.
3) Add one of Powder Pillow(P) to each kit, which is
distilled water and sample add. Cap tightly and shake the
vials vigorously for 15 seconds to mix them.
4) Wait 2 minutes for maximum color development. At end
of 2 minute waiting, insert the vials into cell holder to read
the result. Please refer to chapter 4. HUMAS Program of
Instrument Manual for test procedure.
Iodine
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Iodine
Iodine
Interferences1) Oxidizer, Br2, ClO2, I2 and O3 in the sample will interfere to the test.
2) High buffered sample is need to adjust pH to 6~7.
3) When oxidized manganese(Mn4+, Mn7+) presents in the sample, perform following.
① Adjust pH to between 6~7.
② Add 3 drops of potassium iodide (30g/L).
③ Mix it and leave for one minute.
④ Add 3 drops of sodium arsenite(5/L).
⑤ Test the sample treated by above procedure.
⑥ For the best result, subtract the value measured by above treatment
from the result measured original testing procedure.
Sample collection and PreservationRinse thoroughly the container(avoid plastic bottle) with deionized or distilled water.
Analyze the sample immediately after collection because free chlorine is strong oxidizing agent
and it is unstable in natural water. Do not use the same sample for testing of free and total
chlorine. When sampling from a tap water, let the water flow for at least 5 minutes to
ensure a representative sample. And don't make a bubble in the sample.
Accuracy and PrecisionWhen 1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more
than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.891g of KMnO4(Potassium Permanganate) in distilled water, and make it 1L
precisely. It becomes 1,000㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎,
so test should be performed within appropriate concentration range.
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Iodine
Item Test No. Cat. No.
l2 Set 100/set 36010
Iodine
Contents
DescriptionQuantity Required
per test(vials)Unit Cat. No.
l2 Powder Pillow(P) 1~2 100Ea/set 36010-10
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Iron, Ferrous
Method 4110Phenanthroine Method
Fe(+2)(0.1 ~ 5mg/L)Scope and Application : water and wastewater
Fe(+2) (0.1 ~ 5mg/L)
1) Add 5㎖ of distilled water to sample cell(round glass vial) to prepare reagent blank test.
2) Add 5㎖ of sample to another sample cell (round glass vial) to be tested using a precise pipet.
3) Add 2ml of S to the each cell and shake gently up and down for 10 times to mix them.
4) Add one of Powder Pillow(P) to each kit, which is distilled water and sample add and release gas for 30 seconds. Cap tightly and shake it vigorously to dissolve the reagent.
5) Open the cap the release the CO2 gas from the vials, and take 10 minutes reaction times.
6) Wipe the vials and place it into cell holder and read the result. Please refer to chapter 4. Operation Guideline of Instrument Manual for test procedure.
Iron, Ferrous
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Iron, Ferrous
Iron, Ferrous
InterferencesWhen the concentration of COD-cr, organic matters is higher than 500 ㎎/ℓ, test result is
higher than the actual one more than 5%. When the concentration of PO4-P is higher than 1mg/L, test result is higher than the actual one more than 5%. When the concentration of Cobalt is higher than 10mg/L, test result is lower than the actual one more than 5%. When the concentration of Cu is lower than 10mg/L, test result has an error within 1%.
Sample Collection and Preservation
Collect the sample using an container of glass or plastic acid-cleaned. It is recommended to perform the test promptly after sample is taken. Accuracy and Precision
When 1㎎/L of standard solution is investigated, check it the difference from the actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 7.02g of FeSO4(NH4)2SO4․6H2O(Ammonium Iron(Ⅱ) Sulfate Hexahydrate) in 20mL of Hydrochloric acid(1+1), and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-Fe(+2) Set 100/set 16020
Iron, Ferrous
Contents
Description Quantity Requiredper test(vials) Unit Cat. No.
Fe(+2) Solution (S) - 1bottle/set 16020-20
Fe(+2) Powder Pillow(P) 1~2 100Ea/set 16020-10
Iron, Ferrous
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Iron, Total
Method 4100Phenanthroine Method
Fe(T)(0.1 ~ 5mg/L)Scope and Application : water and wastewater
Fe(T) (0.1 ~ 5mg/L)
1) Add 5ml of distilled water to a sample cell (round glass vial) to prepare reagent blank test.
2) Add 5ml of sample to another sample cell (round glass vial) to be tested using a precise pipet.
3) Add one of Powder Pillow(P) and close them with cap, shake the vials up and down for 30 seconds gently to mix them.
4) Leave it for 5 minutes. 5) Wipe and place the cells into the cell holder within 5 minutes to read the results. Please refer to chapter 4. Operation Guideline of Instrument Manual for test .
Iron, Total
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Iron, Total
Iron, Total
InterferencesIf sample to be tested is colorful, other than transparent, it can affect to the data as well as
floating substances. It should be filtered for the best result before testing. Also strong oxidizing and reducing agents in the sample can have negative affect to the data. Calcium is not effect at less than 10,000 mg/L as CaCO3 and Chloride is not effect at less than 180,000 mg/L. Magnesium is not work as an interference lower than 100,000mg/L.
Sample Collection and Preservation
Collect sample using a acid-cleaned glass or plastic container. It is recommended to perform the test promptly after sample is taken. If not possible, add 2mL of Conc. Nitric acid to it per one 1L of the sample to control the pH under 2. It can be preserved for more than 6 months at room temperature. Before testing, adjust the pH to between 3 and 5 with 5N sodium hydroxide solution. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of nitric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L Accuracy and Precision
When 1㎎/L of standard solution is investigated, check it the difference from the actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 7.02g of FeSO4(NH4)2SO4․6H2O(Ammonium Iron(Ⅱ) Sulfate Hexahydrate) in 20mL of Hydrochloric acid(1+1), and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 1㎎/L, so test should be performed within appropriate concentration range.
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Iron, Total
Item Test No. Cat. No.
HS-Fe(T) Set 100/set 16010
Iron, Total
Contents
Description Quantity Requiredper test(vials) Unit Cat. No.
Fe(T) Powder Pillow-1 1~2 100Ea/set 16030-10
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Manganese
Periodate oxidation Method
Mn(0 ~ 20mg/L)
Scope and Application : water and wastewater
Mn (0 ~ 20mg/L)
1) Add 10ml of distilled water to sample cell (round glass
vial) to prepare reagent blank test.
2) Add 10ml of sample to another sample cell (round glass
vial) to be tested using a precise pipet.
3) Add Powder Pillow-1(P1) to the each cell, and shake them
intensively to dissolve completely the reagent. (About
30seconds)
4) Open them, add Powder Pillow-2(P2) to the each cell, and
shake them intensively to dissolve completely the reagent.
5) After 2 minute reaction, wipe the kits and place it into
cell holder and read the result. Please refer to chapter 4.
HUMAS Program of Instrument Manual for test procedure.
Manganese
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Manganese
Manganese
Interferences If sample to be tested is colorful, other than transparent, it can affect to the actual data.
Floating substance in the sample works as an interference to the test. It should be measured with
5 minute after testing, and the time is very important for the best results. If there are some
Calcium(Ca, 700mg/L), Chloride(Cl-, 70,000mg/L) and Magnesium(Mg, 100,00mg/L) in the
sample, the measured concentration can be higher than the actual concentration. The
concentration is lower than iron, 5㎎/L, it does not work as an interference.
Sample Collection and PreservationCollect samples using an acid-cleaned glass and avoid plastic container and analyze
it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated nitric
acid to pH of less than 2 (about 2㎖ per liter). It can be stored at 4℃. Before analyzing,
warm sample to room temperature and adjust pH to neutralization with sodium hydroxide
solution(5N). In case of that sample contains suspended substances, take the sample while
stirring to make the floating materials are even in it.
※ Compensation as adding the reagent Ex.) When sample which has 2ml of nitric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more
than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Make a standard solution to check accuracy and calibration by commercial standard
solution, 1,000mg/L.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.01㎎/L, so
test should be performed within appropriate concentration range.
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Manganese
Item Test No. Cat. No.
HS-Mn Set 100/set 13010
Manganese
Contents
DescriptionQuantity Required
per test(vials)Unit Cat. No.
Mn Powder Pillow-1(P1) - 100Ea/set 13010-10
Mn Powder Pillow-2(P2) - 100Ea/set 13010-11
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Nickel
Method 6500PAN Method
Ni(0.05 ~ 2mg/L)Scope and Application : water and waste water
Nickel (0.05 ~ 2mg/L)
1) Add 10ml of distilled water to a sample cell(round glass vial) to prepare reagent blank test.
2) Add 10ml of sample to another sample cell (round glass vial) to be tested using a precise pipet.
3) Add one of Powder Pillow-1(P1) to each cell, which is distilled water and sample add. Cap tightly and shake it vigorously to dissolve it.
4) Add 0.5ml of S to each cell. Cap tightly and shake it up and down slowly for 10 times to mix it.
5) Wait 15minutes for reaction.
Nickel
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Nickel
6) Add one of Powder Pillow-2(P2) to each cell, which is distilled water and sample add. Cap tightly and shake It gently to dissolve it.
7) After 1minute reaction, wipe the cells and place vials into cell holder and read the result. Please refer to chapter 4. Operation Guideline of Instrument Manual for test procedure.
Nickel
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Nickel
Interferences
Substance Conc.(mg/L) Substance Conc.(mg/L) or %
Al 60 K 500
Ca 1,000(as CaCO3) Mg 400
Cd 30 Mn 45
Cl 8000 Mo 60
Complex reagents All range Na 5000
Cr(+3) 20 Pb 30
Cr(+6) 80 Zn 40
Cu 15 Buffer reagents Pre-treatment
F(-) 20
Co All rangeFe(+2) All range
Fe(+3) 30
Sample Collection and Preservation
Take samples using a plastic container cleaned with acids and analyze it promptly for good result. If prompt analysis is not possible, add 5㎖ of concentrated nitric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 6 months at room temperature. Before analyzing, adjust pH of sample to neutral range. ※ Compensation as adding the reagent Ex.) When sample which has 5ml of sulfuric acid add for preservation, is tested in 30㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1000㎖ + 5㎖ = 1005㎖○ Calculating compensation value = 1005/1000 =1.005○ Actual Concentration 30㎎/L × 1.005 = 29.85㎎/L
Accuracy and PrecisionWhen 1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionA commercial standard solution(1000㎖/l) is recommend to dilute proper
concentration.
Detection LimitErrors are more frequent if the concentration of a sample is lower than 0.05㎎/L.
Nickel
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Nickel
Item Test No. Cat. No.
HS-Ni Set 100/set 42010
Nickel
Contents
Description Quantity Requiredper test Unit Cat. No.
Ni Powder Pillow-1(P1) 1~2 100Ea/set 42010-10
Ni Powder Pillow-2(P2) 1~2 100Ea/set 42010-11
Ni Solution(S) - 1bottle/set 42010-20
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Nitrogen, Ammonia
Method 2800Indophenol Method
NH3(N)-U(0.03 ~ 1mg/L)Scope and Application : surface water and ground water
NH3(N)-U (0.03 ~ 1mg/L)
1) Add 10ml of distilled water to NH3(N)-U Kit to prepare reagent blank test.
2) Add 10ml of sample to NH3(N)-U Kit to be tested using a precise pipet.
3) Add 0.4ml of S-1 to each kit, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.
4) Add 0.5ml of S-2 to each kit, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.
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5) Add Powder Pillow(P) and shake them up and down slowly for 10 times to mix them.
6) Place the kits in the reactor(HS-R 200) for 10 minutes at 100℃. (Make the reactor's temperature to 100℃ in advance)
7) After the time is over, remove the kits from the reactor, and cool it the room temperature with cold water such as tap water. (Cooling time : 90 seconds)
8) Wipe the kits and place it into cell holder and read the result. Please refer to chapter 4. Operation Guideline of Instrument Manual for test procedure.
Nitrogen, Ammonia
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Nitrogen, Ammonia
Nitrogen, Ammonia
Sample Collection and PreservationCollect samples using a glass and plastic container and analyze it promptly for good
result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. If chlorine presents in the samples, add one drop of Sodium Thiosulfate(0.1N) for each 0.3mg/L Cl2 per 1L sample.※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 0.5㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.3819g of NH4Cl(Ammonium Chloride) which has been dried for more than 5 hrs at 100 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.03 mg/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-NH3(N)-U Set 25/set 06031
Nitrogen, Ammonia
Contents
Description Quantity Requiredper test Unit Cat. No.
NH3(N)-U Kit 1~2 25Ea/set 06031-01
NH3(N)-U Powder Pillow(P) 1~2 25Ea/set 06031-10
NH3(N)-U Solution(S-1) - 1bottle/set 06031-20
NH3(N)-U Solution(S-2) - 1bottle/set 06031-21
Nitrogen, Ammonia
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Nitrogen, Ammonia
Method 2810Nessler Method
NH3(NW)-L(0.2 ~ 6mg/L)Scope and Application : water and wastewater
NH3(N)-L (0.2 ~ 6mg/L)
1) Add 3ml of distilled water to sample cell (round glass vial) to prepare reagent blank test.
2) Add 3ml of sample to another sample cell (round glass vial)to be tested using a precise pipet.
3) Add 2ml of S1 to each cell.
4) Add 4 drops of S2 to each cell.
5) Cap the vials tightly, shake it up and down slowly for 10 times to mix them.
Nitrogen, Ammonia
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Nitrogen, Ammonia
5) Open the cells and add 1ml of S3 to each cell and shake it up and down for 10 times.
6) Perform the test after 3minutes. Please refer to Chapter 4. Operation Guideline of Instrument Manual for test procedure.
Nitrogen, Ammonia
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Nitrogen, Ammonia
Nitrogen, Ammonia
Sample Collection and PreservationCollect samples using a glass and plastic container and analyze it promptly for good
result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N approximately). In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. If chlorine presents in the samples, add one drop of Sodium Thiosulfate(0.1N) for each 0.3mg/L Cl2 per 1L sample. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 3㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.3819g of NH4Cl(Ammonium Chloride) which has been dried for more than 5 hrs at 100 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.2 mg/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-NH3(N)-L Set 100/set 06022
Nitrogen, Ammonia
Contents
Description Quantity Requiredper test Unit Cat. No.
NH3(N)-L Solution(S-1) - 1bottle/set 06022-20
NH3(N)-L Solution(S-2) - 1bottle/set 06022-21
NH3(N)-L Solution(S-3) - 1bottle/set 06022-22
Nitrogen, Ammonia
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Method 2811Nessler Method
NH3(NW)-H(2 ~ 60mg/L)Scope and Application : water and wastewater
NH3(N)-H (2 ~ 60mg/L)
1) Add 0.5ml of distilled water to sample cell (round glass vial) to prepare reagent blank test.
2) Add 0.5ml of sample to another sample cell (round glass vial) to be tested using a precise pipet.
3) Add 6ml of S-1 to each sample cell.
4) Add 4 drops of S-2 to each sample cell.
4) Cap the vials tightly, shake it up and down slowly for 10 times to mix it.
Nitrogen, Ammonia
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Nitrogen, Ammonia
5) Open it and add 1ml of S-3 to each sample cell and shake them up and down for 10 times.
6) After 3 minute reaction, wipe and place the cell into cell holder to read the result. Please refer to Chapter 4. Operation Guideline of Instrument Manual for test procedure.
Nitrogen, Ammonia
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Nitrogen, Ammonia
Nitrogen, Ammonia
Sample Collection and PreservationCollect samples using a glass and plastic container and analyze it promptly for good
result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N approximately). In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. If chlorine presents in the samples, add one drop of Sodium Thiosulfate(0.1N) for each 0.3mg/L Cl2 per 1L sample. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 30㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.3819g of NH4Cl(Ammonium Chloride) which has been dried for more than 5 hrs at 100 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 2 mg/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-NH3(N)-H Set 100/set 06024
Nitrogen, Ammonia
Contents
Description Quantity Requiredper test Unit Cat. No.
NH3(N)-H Solution(S-1) - 1bottle/set 06024-20
NH3(N)-H Solution(S-2) - 1bottle/set 06024-21
NH3(N)-H Solution(S-3) - 1bottle/set 06024-22
Nitrogen, Ammonia
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Nitrogen, Nitrate
Method 2200
Cadmium reduction Method
NO3(N)-L(0.2 ~ 5mg/L)
Scope and Application : water and wastewater
NO3(N)-L (0.2 ~ 5mg/L)
1) Add 5ml of distilled water to sample cell(round glass vial)
to prepare reagent blank test.
2) Add 5ml of sample to another sample cell (round glass
vial)to be tested using a precise pipet.
3) Add Powder Pillow(P) of one to the each sample cell,
Which is distilled water and sample add.
4) Shake them vigorously for one minute.
Nitrogen, Nitrate
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Nitrogen, Nitrate
Nitrogen, Nitrate
5) Leave them for 5 minutes.
6) After reaction for 5 minutes, perform the test . Please
refer to Chapter 4. HUMAS Program for test procedure of
Instrument Manual.
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Nitrogen, Nitrate
Nitrogen, Nitrate
Interferences
If sample to be tested is colorful, other than transparent, it can affect to the data as well as
floating matters. The analysis should be performed within 3 minutes after the test completed. If the
duration is more than 5 minutes, it can affect to the data. Also strong acid and alkaline range can
have negative affect to the data. Some chloride(Cl-) in the sample, the measured concentration
can be lower than the actual concentration. The concentration is lower than 100㎎/L, it does
not work as an interference. Also, ferric ion and nitrite ion may affect negatively to the result.
Sample Collection and Preservation
Collect samples using a glass container and analyze it promptly for good result. If
prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2
(about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to
room temperature and adjust pH to neutralization with sodium hydroxide solution. In case of
that sample contains suspended substances, take the sample while stirring to make the
floating materials are even in it.
※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and Precision
When 1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more
than 7 times and get a standard deviation and arithmetic mean.
Standard Solution
Dissolve 0.7218g of KNO3(Potassium Nitrate) which has been dried for more than 24 hrs at
105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution. Add 2mL of chloroform and keep it in cool and dark place. Then it is stable for 6 months.
Detection Limit
Errors are more frequent if the concentration of a sample is lower than 0.2㎎/L, so
test should be performed within appropriate concentration range.
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Nitrogen, Nitrate
Item Test No. Cat. No.
HS-NO3(N)-L Set 100/set 05020
Nitrogen, Nitrate
Contents
Description Quantity Requiredper test(vials) Unit Cat. No.
NO3(N)-L Powder Pillow(P) - 100Ea/set 05020-10
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Method 2210Brucine Method
NO3(N)(1 ~ 15mg/L)Scope and Application : water and wastewater
NO3(N)-H (1 ~ 15mg/L)
1) Add 0.5ml of distilled water to NO3(N)-H Kit to prepare reagent blank test.
2) Add 0.5ml of sample to NO3(N)-H Kit to be tested using a precise pipet.
3) Add 0.5ml of S to the each Kit, which is distilled water and sample add.
4) Shake up and down for 10 times to mix them. (Be careful with the hot kits)
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5) Put the kits to reactor(HS-R 200) and heat it for 20 minutes at 100℃. Place the plastic cover on the reactor. (Make the reactor's temperature to 100℃ in advance)
6) After heating reaction, take the kits from the reactor and cool it to room temperature for 2 minutes with cold water such as tap water. Perform measurement with 3 minutes, I it is very important for the best results.
7) Perform the test . Please refer to Chapter 4. HUMAS Program for test procedure.
Nitrogen, Nitrate
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Nitrogen, Nitrate
InterferencesIf there is some chloride(Cl-) in the sample, the measured concentration can be higher
than the actual concentration. The concentration is lower than 10,000㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride. If the kits under testing with NO3(N)-H Kit is turned to color from colorlessness before adding S, it means that has interference. Please contact manufacture.
Sample Collection and PreservationCollect samples using a glass container and analyze it promptly for good result. If
prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N). In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionDissolve 0.7218g of KNO3(Potassium Nitrate) which has been dried for more than 24 hrs at
105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.Add 2mL of chloroform and keep it in cool and dark place. Then it is stable for 6 months.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 1.0㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-NO3(N)-H Set 25/set 05010
Nitrogen, Nitrate
Contents
Description Quantity Requiredper test Unit Cat. No.
NO3(N)-H Kit 1~2 25Ea/set 05010-01
NO3(N)-H Solution (S) - 1bottle/set 05010-20
Nitrogen, Nitrate
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Method 2220Chromatropic Method
NO3(N)-CA(0.5 ~ 30mg/L)Scope and Application : water and wastewater
NO3(N)-CA (0.5 ~ 30mg/L)
1) Add 0.5ml of distilled water to NO3(N)-CA Kit to prepare reagent blank test. (Don't add any reagent to this vial)
2) Add 0.5ml of sample to NO3(N)-CA Kit to be tested using a precise pipet.
3) After cap tightly, shake them which sample and distilled water add up and down for 10 times. Add one of Powder Pillow-1 to the kit, only added sample .
4) Cap tightly and shake the kit slowly for 10 times.
5) Leave them for reaction for 10 minutes.
6) After 10 minutes, start get measurement. Please refer to Chapter 4. HUMAS Program for test procedure.
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InterferencesIf there is some chloride(Cl-) in the sample, the measured concentration can be higher
than the actual concentration. The concentration is lower than 500㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride. The substance in the below table lie below the limit at which the determination is interfered with.
Substance Conc.(mg/L) Substance Conc.(mg/L) or %
Barium 1 Copper All range
NO2 12
Sample Collection and PreservationCollect samples using a glass container and analyze it promptly for good result. If
prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N). In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 30㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionDissolve 0.7218g of KNO3(Potassium Nitrate) which has been dried for more than 24 hrs at
105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.
Nitrogen, Nitrate
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Item Test No. Cat. No.
HS-NO3(N)-CA Set 25/set 05030
Nitrogen, Nitrate
Contents
Description Quantity Requiredper test Unit Cat. No.
NO3(N)-CA Kit 1~2 25Ea/set 05030-01
NO3(N)-CA Powder Pillow(P) 1~2 25Ea/set 05030-10
Nitrogen, Nitrate
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Method 2400NED Method
NO2(N)-L(0.1 ~ 1mg/L)Scope and Application : water and wastewater
NO2(N)-L (0.1 ~ 1mg/L)
1) Add 2ml of distilled water to sample cell (round glass vial) to prepare reagent blank test.
2) Add 2ml of sample to another sample cell (round glass vial) to be tested using a precise pipet.
3) Add 3ml of S to each sample cell. Cap tightly and shake slowly the sample cells up and down for 10 times.
4) Add one of Powder Pillow (P) to each sample cell.
Nitrogen, Nitrite
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Nitrogen, Nitrite
5) Cap tightly and shake each kit vigorously for 15 seconds.
6) Leave them for reaction for 10 minutes.
7) After 10 minutes, start get measurement. Please refer to Chapter 4. Operation Guideline for test procedure.
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Nitrogen, Nitrite
InterferencesStrong oxidant and reducing substance in the sample will interfere. If samples contain
greater than 600mg/L Alkalinity, it can make lower reading than actual data.
Sample Collection and PreservationCollect samples using a glass and plastic container and analyze it promptly for good
result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L Accuracy and Precision
When 0.5㎎/L of standard solution is investigated, check it the difference from the actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 1.232g of NaNO2(Sodium Nitrite) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 250㎎/ℓ of standard solution. Add 2mL of chloroform and keep it in cool and dark place.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-NO2(N)-L Set 100/set 04012
Nitrogen, Nitrite
Contents
Description Quantity Requiredper test Unit Cat. No.
NO2(N)-L Solution (S) - 1bottle/set 04012-20
NO2(N)-L Powder Pillow(P) 1~2 100Ea/set 04012-10
Nitrogen, Nitrite
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Nitrogen, Nitrite
Method 2410FSS Method
NO2(N)-H(5 ~ 150mg/L)Scope and Application : water and wastewater
NO2(N)-H (5 ~ 150mg/L)
1) Add 10ml of distilled water to sample cell (round glass vial) to prepare reagent blank test.
2) Add 10ml of sample to be tested another sample cell ( round glass vial) using precise pipet.
3) Add 1ml of S to each sample cell. Cap tightly and shake slowly the sample cells up and down for 10 times.
4) Add one of Powder Pillow (P) to each sample cell.
5) Close it with the cap and shake them up and down slowly for 10 times to mix them.
6) After 10 minutes, wipe the cells and place it into cell holder and read the result. Please refer to chapter 4. Operation Guideline of Instrument Manual for test procedure.
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Nitrogen, Nitrite
Nitrogen, Nitrite
InterferencesStrong oxidant and reducing substance in the sample will interfere. If samples contain
greater than 600mg/L Alkalinity, it can make lower reading than actual data.
Sample Collection and PreservationCollect samples using a glass and plastic container and analyze it promptly for good
result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 30㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L Accuracy and Precision
When 100㎎/L of standard solution is investigated, check it the difference from the actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 1.232g of NaNO2(Sodium Nitrite) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 250㎎/ℓ of standard solution. Add 2mL of chloroform and keep it in cool and dark place.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 5㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-NO2(N)-H Set 100/set 04014
Nitrogen, Nitrite
Contents
Description Quantity Requiredper test Unit Cat. No.
NO2(N)-H Solution (S) - 1bottle/set 04014-20
NO2(N)-H Powder Pillow(P) 1~2 100Ea/set 04014-10
Nitrogen, Nitrite
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Nitrogen, Total
Method 2000Cadmium Reduction Method
TN-U(0.2 ~ 5mg/L)Scope and Application : water and wastewater
TN-U (0.2 ~ 5mg/L)
1) To prepare the reagent blank test, add 2ml of distilled water to TN-U Kit.
2) Take 2㎖ of a sample to be tested using a precise pipette and add to different TN-U Kit.
3) Add Powder Pillow-1(P1) of one to each TN-U Kit(vial).
4) Close it with cap and shake it up and down slowly for 10 times to mix it.
Nitrogen, Total
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Nitrogen, Total
5) Put the kits to reactor(HS-R 200) and heat it for 30 minutes at 120℃. Place the plastic cover on the reactor. After heating reaction, cool the kits to room temperature with cold water such as tap water. (The heating time is very important for the best result)
6) Add Power Pillow-2(P2) of one to each kit and shake vigorously the vials for one minute.
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Nitrogen, Total
7) Add Power Pillow-3(P3) of one to each kit and shake the vials up and down for 10 times to mix them.
8) After leave them for 5 minutes and measure it. See Chapter 4. HUMAS Proram of the Instrument Manual and measure the samples.
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Nitrogen, Total
Nitrogen, Total
InterferencesThe colored solution and suspended substances in the sample will interfere. Also,
strong oxidizing and reducing materials will interfere. Chloride(Cl-) concentration above 100㎎/L will cause low results. If ferric ion and nitrite ion in the samples, those work as interference. It should be measured within 3 minutes after testing for the best results. Collection and Preservation
Collect samples using a glass container and analyze it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and neutralize (pH 5 approximately) with 5N Sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of Sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,002㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 2㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionDissolve 0.7218g of KNO3(Potassium Nitrate) which has been dried for more than 24 hrs at
105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution. Add 2mL of chloroform and keep it in cool and dark place. Then it is stable for 6 months.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.2㎎/L.
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Nitrogen, Total
Item Test No. Cat. No.
HS-TN-U Set 25/set 02011
Nitrogen, Total
Contents
Description Quantity Requiredper test Unit Cat. No.
TN-U Kit 1~2 25Ea/set 02011-01
TN-U Powder Pillow-1(P1) 1~2 25Ea/set 02011-10
TN-U Powder Pillow-2(P2) 1~2 25Ea/set 02011-11
TN-U Powder Pillow-3(P3) 1~2 25Ea/set 02011-12
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Method 2020Chromotropic acid Method
TN-L(CA)(1 ~ 50mg/L)Scope and Application : water and wastewater
TN-L(CA) (1 ~ 50mg/L)
1) Add 2ml of distilled water to TN-L(CA) Kit(Black Cap) to prepare reagent blank test.
2) Add 2ml of sample to another TN-L(CA) Kit(Black Cap) to be tested using a precise pipet.
3) Add Powder Pillow-1(P1) of one to the each Kit(Black Cap), which is distilled water and sample add.
4) Close it with the cap and shake it up and down slowly for 10 times to mix them.
Nitrogen, Total
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5) Put the kits to reactor(HS-R 200) and heat it for 30 minutes at 120℃. Place the plastic cover on the reactor. (Make the reactor's temperature to 150℃ in advance) ※When external temperature is too low, white sediments which may affect to data can be made.
6) After heating reaction, take the kits from the reactor and cool it to room temperature with cold water such as tap water. Then, add Powder Pillow-2(P2) and shake it vigorously for 30 seconds to dissolve it completely. Then make it react for 3 minutes.
7) Add Powder Pillow-3(P3) and shake them up and down for 10 times to mix them. Then make the kits react for 2 minutes.
8) Take 2ml of the sample from the TN-L(CA) (Blcak cap) and add respectively to two TN-L(CA) Kits (White cap). Shake it up and down for 10 times to mix them. (Be careful with the hot kits)
Nitrogen, Total
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Nitrogen, Total
Nitrogen, Total
9) After reaction for 10 minutes, perform the test . Please refer to Chapter 4. Operation Guideline for test procedure of the Instrument Manual.
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Nitrogen, Total
Nitrogen, Total
InterferencesIf there is some chloride(Cl-) in the sample, the measured concentration can be higher
than the actual concentration. The concentration is lower than 800㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride. Sample containing more than 0.5mg/L ferrous, it works as a interference and can be caused higher result than actual concentration. Not exceeding pH 12 of the sample, it does not work as an interference. Sample containing more than 300mg/L COD, it may cause to higher result.
Sample Collection and PreservationCollect samples using a glass container and analyze it promptly for good result. If
prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and neutralize pH to 7 with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionDissolve 0.7218g of KNO3(Potassium Nitrate) which has been dried for more than 24 hrs at
105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution. Add 2mL of chloroform and keep it in cool and dark place. Then it is stable for 6 months.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 1.0㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-TN-L(CA) Set 25Ea/set 02022
Nitrogen, Total
Contents
Description Quantity Requiredper test(vials) Unit Cat. No.
TN-L(CA) Kit 1~2 25Ea/set 02022-01
TN-L(CA) Powder Pillow-1 1~2 25Ea/set 02022-10
TN-L(CA) Powder Pillow-2 1~2 25Ea/set 02022-11
TN-L(CA) Powder Pillow-3 1~2 25Ea/set 02022-12
Nitrogen, Total
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Method 2021Chromotropic acid Method
TN-H(CA)(10 ~ 100mg/L)Scope and Application : water and wastewater
TN-H(CA) (10 ~ 100mg/L)
1) Add 0.5ml of distilled water to TN-H(CA) Kit(Black cap) to prepare reagent blank test.
2) Add 0.5ml of sample to another TN-H(CA) Kit(Black cap) to be tested using a precise pipet.
3) Add Powder Pillow-1(P1) of one to the each Kit(Black cap), which is distilled water and sample add.
4) Close it with the cap and shake it up and down slowly for 10 times to mix them.
Nitrogen, Total
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Nitrogen, Total
Nitrogen, Total
5) Put the kits to reactor(HS-R 200) and heat it for 30 minutes at 120℃. Place the plastic cover on the reactor. (Make the reactor's temperature to 150℃ in advance) ※ When external temperature is too low, white sediments which may affect to data can be made
6) After heating reaction, take the kits from the reactor and cool it to room temperature with cold water such as tap water. Then, add Powder Pillow-2(P2) and shake it vigorously for 30 seconds to dissolve it completely. Then make it react for 3 minutes.
7) Add Powder Pillow-3(P3) and shake them up and down for 10 times to mix them. Then make the kits react for 2 minutes.
8) Take 1ml of the sample from the TN-H(CA) (Black cap) and add respectively to two TN-H(CA) Kits (White cap). Shake it up and down for 10 times to mix them. (Be careful with the hot kits)
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Nitrogen, Total
Nitrogen, Total
9) After reaction for 10 minutes, perform the test . Please refer to Chapter 4. Operation Guideline for test procedure of the Instrument Manual.
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Nitrogen, Total
Nitrogen, Total
InterferencesIf there is some chloride(Cl-) in the sample, the measured concentration can be higher
than the actual concentration. The concentration is lower than 800㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride. Sample containing more than 0.5mg/L ferrous, it works as a interference and can be caused higher result than actual concentration. Not exceeding pH 12 of the sample, it does not work as an interference. Sample containing more than 300mg/L COD, it may cause to higher result.
Sample Collection and PreservationCollect samples using a glass container and analyze it promptly for good result. If
prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and neutralize pH to 7 with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 50㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard SolutionDissolve 0.7218g of KNO3(Potassium Nitrate) which has been dried for more than 24 hrs at
105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution. Add 2ml of chloroform and keep it in cool and dark place. Then it is stable for 6 months.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 10㎎/L, so
test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-TN-H(CA) Set 25Ea/set 02024
Nitrogen, Total
Contents
Description Quantity Requiredper test Unit Cat. No.
TN-H(CA) Kit 1~2 25Ea/set 02024-01
TN-H(CA) Powder Pillow-1 1~2 25Ea/set 02024-10
TN-H(CA) Powder Pillow-2 1~2 25Ea/set 02024-11
TN-H(CA) Powder Pillow-3 1~2 25Ea/set 02024-12
Nitrogen, Total
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Orthophosphate
Orthophosphatel
Method 3020Ascorbic acid Method
PO4(P)-L(0.01 ~ 3mg/L)Scope and Application : water and wastewater
PO4(P)-L (0.01 ~ 3mg/L)
1) Add 5ml of distilled water to sample cell(round glass cell) to prepare reagent blank test.
2) Add 5ml of sample to another sample cell(round glass cell) to be tested using a precise pipet.
3) Add 6ml of S to each sample cell.
4) Add one of Powder Pillow(P) to each sample cell, which is distilled water and sample add.
5) Cap tightly and shake it up and down slowly for 10 times to mix them. This test needs reaction time for 10 minutes.
6) After 10 minute reaction, wipe the cells and place it into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.
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Orthophosphate
Orthophosphate
Interferences Ferric, copper, and silicate ion in the sample, that are not work as an interferences. However,
high concentration of ferric ion may make deposits and become interferences. When strong oxidizing or reducing agent is contained, it will may interfere. If the concentration of Arsenic is higher than the one of TP, it can affect on the test as an interference. The concentration level of that in seawater has no effective. When the concentration of Cl- is higher than 7,000 ㎎/ℓ, test value shall be lower than the actual one. It should be diluted to proper concentration of chloride for the best results.
Sample Collection and PreservationCollect samples using a glass and plastic container and analyze it promptly for good
result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N approximately). In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.439g of KH2PO4(Potassium Dihydrogenphosphate) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.01㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-PO4(P)-L Set 100/set 07012
Orthophosphate
Contents
Description Quantity Requiredper test Unit Cat. No.
PO4(P)-L Solution(S) - 1 bottle/set 07012-20
PO4(P)-L Powder Pillow(P) 1~2 100Ea/set 07012-10
Orthophosphate
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Orthophosphate
Method 3030Molybdo Vanadate Method
PO4(P)-H(1 ~ 15mg/L)Scope and Application : water and wastewater
PO4(P)-H (1 ~ 15mg/L)
1) Add 5ml of distilled water to (round glass cell) to prepare reagent blank test.
2) Add 5ml of sample to another sample cell (round glass cell) to be tested using a precise pipet.
3) Add 6ml of S to each sample cell.
4) Cap the cells tightly, and shake it up and down for 10 times to mix the cells.
5) After 10 minute reaction, wipe the cells and place it into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.
Orthophosphate
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Orthophosphate
Orthophosphate
InterferencesFerric, copper, and silicate ion in the sample, that are not work as an interferences. However,
upper concentration of 100 mg/L ferric ion may make deposits and become interferences. When strong oxidizing or reducing agent is contained, it will may interfere. When the concentration of Cl- is higher than 20,000 ㎎/ℓ, test value shall be lower than the actual one. It should be diluted to proper concentration of chloride for the best results. Sample Collection and Preservation
Collect samples using a glass and plastic container and analyze it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.439g of KH2PO4(Potassium Dihydrogenphosphate) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-PO4(P)-H Set 100/set 07014
Orthophosphate
Contents
Description Quantity Requiredper test Unit Cat. No.
PO4(P)-H Solution (S) - 1 bottle/set 07014-20
Orthophosphate
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Phenol
Method 4500
Direct photometric Method
Phenol(0.1 ~ 10mg/L)
Scope and Application : water and wastewater
Phenol (0.1 ~ 10mg/L)
1) Add 10ml of distilled water to sample cell (round glass
cell) to prepare reagent blank test.
2) Add 10ml of sample to another sample cell(round glass
cell) to be tested using a precise pipet.
3) Add 0.2ml of S-1 to each cell. Cap tightly and shake it
up and down slowly for 10 times to mix it.
4) Add 0.2ml of S-2 to each kit, Cap tightly and shake it
up and down slowly for 10 times to mix it.
5) Add 0.2ml of S-3 to each cell. Cap tightly and shake it
up and down slowly for 10 times to mix it.
6) Take reaction time for 15 minutes, and wipe the cells
and place it into cell holder and read the result. Please
refer to chapter 4. HUMAS Program of Instrument Manual for
test procedure.
Phenol
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Phenol
Phenol
InterferencesStrong oxidant and reducing substance in the sample will interfere. For the best result, adjust
the pH of sample to 3~11.5. If sample to be tested is colorful, other than transparent, it can affect to
the actual data.
Sample Collection and PreservationCollect samples using glass and plastic container and analyze it promptly for good
result. A kind of phenol is decomposed easily by reducing and oxidizing reagent and bacteria,
so, it should be analyzed within 4 hours after collecting. If prompt analysis is not possible,
add 10 % phosphorous acid to pH of less than 4. And, add 5g of Copper sulfate per 1L of
the collected sample. It can be stored for 24 hours at dark place.
Accuracy and PrecisionWhen 1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more
than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 1g of Phenol in distilled water, and make it 1L precisely. It becomes 1000㎎/ℓ of
standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so
test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-Phenol Set 100Ea/set 09012
Phenol
Phenol
Contents
DescriptionQuantity Required
per test(vials)Unit Cat. No.
Phenol Solution (S-1) - 1bottle/set 09012-20
Phenol Solution (S-2) - 1bottle/set 09012-21
Phenol Solution (S-3) - 1bottle/set 09012-22
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Phosphorous, Total
Method 3000Ascorbic acid Method
TP-L(0.01 ~ 3mg/L)Scope and Application : water and wastewater
TP-L (0.01 ~ 3mg/L)
1) Add 5ml of distilled water to TP-L Kit to prepare reagent blank test.
2) Add 5ml of sample to TP-L Kit to be tested using a precise pipet.
3) Add one of Powder Pillow-1(P1) to each kit, which is distilled water and sample add.
4) Cap the kits tightly and shake it up and down slowly for 10 times to mix it.
Phosphorous, Total
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Phosphorous, Total
Phosphorous, Total
5) Put the kits to reactor(HS-R 200) and heat it for 30 minutes at 120℃. Place the plastic cover on the reactor. (Make the reactor's temperature to 120℃ in advance)
6) After heating it for 30 minutes at 120 ℃, cool it off by cold water until it reaches to room temperature. Then, add 2ml of S-1 to each kit and shake the vials up and down slowly for 10 times to mix it.
7) Add 2mL of S-2 and close it with cover. Shake it up and down slowly for 10 times.
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Phosphorous, Total
Phosphorous, Total
8) Add one of Powder Pillow-2(P2) and close it with cover. Shake it up and down slowly for 10 times.
9) After 10 minute reaction, wipe and place the vials into cell holder to read the result. Please refer to Chapter 4. HUMAS Program of Instrument Manual for test procedure.
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Phosphorous, Total
Phosphorous, Total
Interferences Ferric, copper, and silicate ion in the sample, that are not work as an interferences. However,
high concentration of ferric ion may make deposits and become interferences. When strong oxidizing or reducing agent is contained, it will may interfere. If the concentration of Arsenic is higher than the one of TP, it can affect on the test as an interference. The concentration level of that in seawater has no effective. When the concentration of Cl- is higher than 7,000 ㎎/ℓ, test value shall be lower than the actual one. It should be diluted to proper concentration of chloride for the best results.
Sample Collection and PreservationCollect samples using a glass and plastic container and analyze it promptly for good
result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N approximately). In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.439g of KH2PO4(Potassium Dihydrogenphosphate) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.01㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-TP-L Set 25/set 03012
Phosphorous, Total
Contents
Description Quantity Requiredper test Unit Cat. No.
TP-L Kit 1~2 25Ea/set 03012-01
TP-L Powder Pillow-1(P1) 1~2 25Ea/set 03012-10
TP-L Powder Pillow-2(P2) 1~2 25Ea/set 03012-11
TP-L Solution(S-1) - 1bottle/set 03012-20
TP-L Solution(S-2) - 1bottle/set 03012-21
Phosphorous, Total
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Phosphorous, Total
Method 3010Molybdo Vanadate Method
TP-H(1 ~ 15mg/L)Scope and Application : water and wastewater
TP-H (1 ~ 15mg/L)
1) Add 5ml of distilled water to TP-H Kit to prepare reagent blank test.
2) Add 5ml of sample to TP-H Kit to be tested using a precise pipet.
3) Add one of Powder Pillow(P) to each kit, which is distilled water and sample add.
4) Cap the kits tightly and shake it up and down slowly for 10 times to mix the vials.
Phosphorous, Total
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Phosphorous, Total
Phosphorous, Total
5) Put the kits to reactor(HS-R 200) and heat it for 30 minutes at 120℃. Place the plastic cover on the reactor. (Make the reactor's temperature to 120℃ in advance)
6) After the time is over, remove the vials from the reactor and cool it to reach room temperature by cold water such tap water. Add 2ml of S-1 to each kit and shake it up and down slowly for 10 times to mix the vials.
7) Add 2ml of S-2 and cap the vials tightly. Then, to mix the solution, shake the vials up and down slowly for 10 times.
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Phosphorous, Total
Phosphorous, Total
8) After 10 minute reaction, wipe and place the vials into cell holder to read results. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.
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Phosphorous, Total
Phosphorous, Total
InterferencesFerric, copper, and silicate ion in the sample, that are not work as an interferences. However,
upper concentration of 100 mg/L ferric ion may make deposits and become interferences. When strong oxidizing or reducing agent is contained, it will may interfere. When the concentration of Cl- is higher than 20,000 ㎎/ℓ, test value shall be lower than the actual one. It should be diluted to proper concentration of chloride for the best results. Sample Collection and Preservation
Collect samples using a glass and plastic container and analyze it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.439g of KH2PO4(Potassium Dihydrogenphosphate) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 1㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-TP-H Set 25Ea/set 03014
Phosphorous, Total
Contents
Description Quantity Requiredper test Unit Cat. No.
TP-H Kit 1~2 25Ea/set 03014-01
TP-H Powder Pillow(P) 1~2 25Ea/set 03014-10
TP-H Solution(S-1) - 1bottle/set 03014-20
TP-H Solution(S-2) - 1bottle/set 03014-21
Phosphorous, Total
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Silica
Method 5500
Molybdosilicate Method
Silica(0.5 ~ 20mg/L)
Scope and Application : water and wastewater
Silica-L (0.5 ~ 20mg/L)
1) Add 5ml of distilled water to sample cell (round glass
cell) to prepare reagent blank test.
2) Add 5ml of sample to another sample cell(round glass
cell) to be tested using a precise pipet.
3) Add Powder Pillow-1(P1) and shake them up and down
slowly for 10 times to mix them.
4) Add Powder Pillow-2(P2) and shake them up and down
slowly for 30 seconds to mix them. And, wait for 10
minutes.
5) Add Powder Pillow-3(P3) and shake them up and down
slowly for 30 seconds to mix them.
6) After 3minute reaction, wipe the cells and place it into
cell holder and read the result. Please refer to chapter
4. HUMAS Program of Instrument Manual for test
procedure.
Silica
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Silica
InterferencesStrong oxidant and reducing substance in the sample will interfere. Large amount of iron,
color turbidity, sulfide and phosphate will interfere.
Sample Collection and PreservationTo collect samples using an plastic container and avoid using glassware as much as
possible and analyze it promptly for good result. If prompt analysis is not possible, it can be
stored for 7days at room temperature. Before, analyzing, warm sample to room temperature.
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more
than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Use commercial standard solution(1000mg/L) to check accuracy. It should be dilute to
proper concentration that is within the range.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.5㎎/L and
higher than 20mg/L, so test should be performed within appropriate concentration range.
Silica
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Silica
Item Test No. Cat. No.
HS-Si-L Set 100Ea/set 44012
Contents
Description Quantity Requiredper test(vials) Unit Cat. No.
Si-L Powder Pillow -1(P1) - 100Ea/set 44012-10
Si-L Powder Pillow -2(P2) - 100Ea/set 44012-11
Si-L Powder Pillow -3(P3) - 100Ea/set 44012-12
Silica
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Silica
Method 5510
Molybdosilicate Method
Silica(20 ~ 100mg/L)
Scope and Application : water and wastewater
Silica-H (20 ~ 100mg/L)
1) Add 5ml of distilled water to sample cell (round glass
cell) to prepare reagent blank test.
2) Add 5ml of sample to another sample cell(round glass
cell) to be tested using a precise pipet.
3) Add Powder Pillow-1(P1) and shake them up and down
slowly for 10 times to mix them.
4) Add Powder Pillow-2(P2) and shake them up and down
slowly for 30 seconds to mix them. And, wait for 10
minutes.
5) Add Powder Pillow-3(P3) and shake them up and down
slowly for 30 seconds to mix them.
6) After 3minute reaction, wipe the cells and place it into
cell holder and read the result. Please refer to chapter
4. HUMAS Program of Instrument Manual for test
procedure.
Silica
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Silica
InterferencesStrong oxidant and reducing substance in the sample will interfere. Large amount of iron,
color turbidity, sulfide and phosphate will interfere.
Sample Collection and PreservationTo collect samples using an plastic container and avoid using glassware as much as
possible and analyze it promptly for good result. If prompt analysis is not possible, it can be
stored for 7days at room temperature. Before, analyzing, warm sample to room temperature.
Accuracy and PrecisionWhen 30㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more
than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Use commercial standard solution(1000mg/L) to check accuracy. It should be dilute to
proper concentration that is within the range.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 20㎎/L and
higher than 100mg/L, so test should be performed within appropriate concentration range.
Silica
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Item Test No. Cat. No.
HS-Si-H Set 100Ea/set 44014
Silica
Contents
Description Quantity Requiredper test(vials) Unit Cat. No.
Si-H Powder Pillow-1(P1) - 100Ea/set 44014-10
Si-H Powder Pillow-2(P2) - 100Ea/set 44004-11
Si-H Powder Pillow-3(P3) - 100Ea/set 44014-12
Silica
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Sulfate
Method 4600Gravimetric MethodSO4-L(5 ~ 50mg/L)
Scope and Application : water and wastewater
SO4-L (5 ~ 50mg/L)
1) Add 10ml of distilled water to sample cell (round glass cell) to prepare reagent blank test.
2) Add 10ml of sample to another sample cell (round glass cell) to be tested using a precise pipet.
3) Add 1ml of S to each sample cell and shake them up and down slowly for 10 times to mix them.
4) Add one of Powder Pillow(P) to each sample cell and shake them up and down slowly for 10 times to mix them.
5) After 2 minutes reaction, wipe and place the cells into cell holder to read the result. Please refer to Chapter 4. HUMAS Program of Instrument Manual for test procedure.
Sulfate
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Sulfate
Sulfate
InterferencesIf there is some silica in the sample, the concentration is lower than 5,00 ㎎/L, it
does not work as an interference. If sample to be tested is colorful, other than transparent, it can affect to the actual data. Floating substance in the sample works as an interference to the test.
Sample Collection and PreservationCollect samples using an acid-cleaned glass and plastic container and analyze it
promptly for good result. If prompt analysis is not possible, it can be stored for 7days at 4℃. Before, analyzing, warm sample to room temperature.
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean. Standard Solution Dissolve 1.813g of K2SO4(Potassium Sulfate) in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution. Also, commercial standard solution is can be used. Detection Limit Errors are more frequent if the concentration of a sample is lower than 5㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-SO4-L Set 100/set 08012
Sulfate
Contents
Description Quantity Requiredper test Unit Cat. No.
SO4-L Solution(S) - 1bottle/set 08012-20
SO4-L Powder Pillow(P) 1~2 100Ea/set 08012-10
Sulfate
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Sulfide
Method 4601Gravimetric Method
SO4-H(10 ~ 70mg/L)Scope and Application : water and wastewater
SO4-H (10 ~ 70mg/L)
1) Add 10ml of distilled water to sample cell (round glass cell) to prepare reagent blank test.
2) Add 10ml of sample to another sample cell (round glass cell) to be tested using a precise pipet.
3) Add 1ml of S to each sample cell and shake them up and down slowly for 10 times to mix them.
4) Add one of Powder Pillow(P) to each sample cell and shake them up and down slowly for 10 times to mix them.
5) After 2 minutes reaction, wipe and place the cells into cell holder to read the result. Please refer to Chapter 4. HUMAS Program of Instrument Manual for test procedure.
Sulfate
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Sulfate
Sulfate
InterferencesIf there is some silica in the sample, the concentration is lower than 5,00 ㎎/L, it
does not work as an interference. If sample to be tested is colorful, other than transparent, it can affect to the actual data. Floating substance in the sample works as an interference to the test.
Sample Collection and PreservationCollect samples using an acid-cleaned glass and plastic container and analyze it
promptly for good result. If prompt analysis is not possible, it can be stored for 7days at 4℃. Before, analyzing, warm sample to room temperature.
Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean. Standard Solution Dissolve 1.813g of K2SO4(Potassium Sulfate) in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution. Also, commercial standard solution is can be used. Detection Limit Errors are more frequent if the concentration of a sample is lower than 5㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-SO4-H Set 100/set 08014
Sulfate
Sulfate
Contents
Description Quantity Requiredper test Unit Cat. No.
SO4-H Solution (S) - 1bottle/set 08014-20
SO4-H Powder Pillow(P) 1~2 100Ea/set 08014-10
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Sulfide
Method 5400Methylene blue Method
S-2(0.5 ~ 5mg/L)Scope and Application : water and wastewater
S-2 (0.5 ~ 5mg/L)
1) Add 2ml of distilled water to sample cell (round glass cell) to prepare reagent blank test.
2) Add 2ml of sample to another sample cell (round glass cell) to be tested using a precise pipet.
3) Add 5ml of S-1 to each cell, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.
4) Add 0.5ml of S-2 to each cell, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.
5) Add 1ml of S-3 to each cell, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.
Sulfide
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Sulfide
Sulfide
6) Add 0.5ml of S-4 to each cell, cap and shake the cells up and down slowly for 10 times.
7) Wait 10 minutes for maximum color development. At end of 2 minute waiting, insert the vial into cell holder to read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.
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Sulfide
Sulfide
Interferences If sample to be tested is colorful, other than transparent, it can affect to the actual data.
Floating substance in the sample works as an interference to the test. A reducing, sulfite, thiosulfate and hydrosulfite in the sample will interfere. High buffered sample is need to dilute proper concentration for the test.
Sample Collection and PreservationCollect samples using an acid-cleaned glass and plastic container and analyze it
promptly for good result.
Accuracy and PrecisionWhen 1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 0.75g of Na29H2O(Sodium Sulfide 9-hydrate, crystal) in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.5㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-S2- Set 100/set 37010
Sulfide
Contents
Description Quantity Requiredper test(vials) Unit Cat. No.
S-2 Solution(S-1) - 1bottle/set 37010-20
S-2 Solution(S-2) - 1bottle/set 37010-21
S-2 Solution(S-3) - 1bottle/set 27010-22
S-2 Solution(S-4) - 1bottle/set 37010-23
Sulfide
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Turbidity
Method 6700Absorption MethodTurb.(0 ~ 400FTU)
Scope and Application : water and wastewate
Turbidity(0 ~ 400FTU)
1) Add 5ml of distilled water to sample cell to prepare reagent blank test.
2) Add 5ml of sample to another sample cell to be tested using a precise pipet.
3) Wipe place the cells into the cell holder to read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.
Turbidity
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Zinc
Method 4200Zincon Method
Zn(0.1 ~ 5mg/L)Scope and Application : water and wastewater
Zn (0.1 ~ 5mg/L)
1) Add 5ml of distilled water to sample cell (round glass cell) to prepare reagent blank test.
2) Add 5ml of sample to another sample cell (round glass cell) to be tested using a precise pipet.
3) Add 2ml of S-1 to each sample cell.
4) Add one of Powder Pillow(P) to each sample cell.
5) Cap the cells tightly and shake the cells up and down gently for 10 times.
Zinc
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Zinc
Zinc
6) Add 0.9ml of S-2 to each cell, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.
7) Add 0.5ml of S-3 to each cell, cap tightly and shake it up and down slowly for 10 times to mix it.
8) After one minute, Wipe the cells and place it into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.
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Zinc
Zinc
Interferences The following substances have been tested for interference and showed not work as interference up to indicated levels.
Substance Conc. (mg/L)
Aluminum 6
Cadmium 0.5
Iron 7
Manganese 5
Nickel 5
Copper 5
Sample Collection and Preservation
Collect samples using an acid-cleaned glass and plastic container and analyze it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution. Floating substance in the sample may will interfere. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L
Accuracy and PrecisionWhen 1㎎/L of standard solution is investigated, check it the difference from the
actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.
Standard Solution Dissolve 1g of Zinc in 10 mL of HNO3(1+1). To eliminate nitric oxidant from the solution, heat the solution after adding some distilled water and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.
Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.
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Item Test No. Cat. No.
HS-Zn Set 100/set 17010
Zinc
Contents
Description Quantity Requiredper test(vials) Unit Cat. No.
Zn Powder Pillow(P1) 1~2 100Ea/set 17010-10
Zn Solution(S-1) - 1bottle/set 17010-20
Zn Solution(S-2) - 1bottle/set 17010-21
Zn Solution(S-3) - 1bottle/set 17010-22
Zinc
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