humas program manual - humas co., ltd. · humas program manual w ater analyzer hs-3300 - 7 -...

H U M A S P R O G R A M M A N U A L

W A T E R A N A L Y Z E R H S -3 3 0 0

HUMAS PROGRAM　MANUAL

WATER ANALYZER　

Analysis Manual



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CategoryHUMAS

ProgramMethod

Range

(mg/L)

Program

NO.

Page

NO.

Aluminum HS-Al ECR 0.01~0.3 5000 2

Cadmium HS-Cd Dithizone 0 ~ 0.5 6100 6

Chloride,LR HS-Cl-L Mercuric Thiocyanate 1~20 5100 10

Chloride,HR HS-Cl-H Mercuric Thiocyanate 20~60 5101 13

Chlorine, Free HS-Cl2(Free) DPD 0.1~2 4310 16

Chlorine, Total HS-Cl2(T) DPD 0.1~2 4300 19

Chromium, Hexavalent HS-Cr(+6) Diphenylcarbazide, DPC 0.1~1 4410 22

Chromium, Total HS-Cr(T) Diphenylcarbazide, DPC 0.1~1 4400 25

Cobalt HS-Co PAN 0.05~2 6400 29

COD(Chemical Oxygen Demand),UR COD-UR Reaction Digestion 5~40 1000 33

COD(Chemical Oxygen Demand),LR COD-LR Reaction Digestion 15~150 1001 37

COD(Chemical Oxygen Demand),MR COD-MR, HR Reaction Digestion 50~1500 1002 41

COD(Mn)(Chemical Oxygen Demand),UR COD-Mn-U Reaction Digestion 0.2~3 1502 45

COD(Mn)(Chemical Oxygen Demand),LR COD-Mn-L Reaction Digestion 0.6~20 1500 49

COD(Mn)(Chemical Oxygen Demand),HR COD-Mn-H Reaction Digestion 20~100 1501 53

Color HS-Color Platium Cobalt 50~1000° 6600 57

Copper HS-Cu Bathocuproine 0.1~5 4000 58

Cyanide HS-CN DP 0.005 ~ 0.5 4900 62

Detergents HS-ABS Crystal violet 0.01~0.3 6200 66

Fluoride HS-F SPANDS 0.1~1.5 4700 70

Hardness(Ca)HS-HD(Ca)(Mg) Calmagite 0.1~4

700073

7001Hardness(Mg)

Hydrogen peroxide,LR HS-H2O2 Titanium sulfate 0.5~30 5300 77

Hydrogen peroxide,HR HS-H2O2 Titanium sulfate 3~300 5301 80

Iodine HS-I2 DPD 0.1~2 5200 83

Iron, Ferrous HS-Fe(+2) Phenanthroine 0.1~5 4110 86

Iron, Total HS-Fe(T) Phenanthroine 0.1~5 4100 89

Manganese HS-Mn Periodate Oxidation 0~ 20 4800 92

Nickel HS-Ni PAN 0.05~2 6500 95

Nitrogen, Ammonia,UR HS-NH3(N)-U Cyanuric acid 0.03~1 2800 99

Nitrogen, Ammonia,LR HS-NH3(N)-L Nessler 0.2~6 2810 103

Nitrogen, Ammonia,HR HS-NH3(N)-H Nessler 2~60 2811 107

Nitrogen, Nitrate, HS-NO3(N)-L Cadmium reduction 0.2~5 2200 111

Nitrogen, Nitrate HS-NO3(N)-H Brucine 1~15 2210 115

Nitrogen, Nitrate,CA HS-NO3(N)-CA Chromatropic acid 0.5~30 2220 119

Nitrogen, Nitrite,LR HS-NO2(N)-L NED 0.1~1 2400 122

Nitrogen, Nitrite,HR HS-NO2(N)-H FSS 5~150 2410 126

Nitrogen, Total,UR HS-TN-U Cadmium Reduction 0.2~5 2000 129

Nitrogen, Total,LR HS-TN-L(CA) Chromatropic acid 1~50 2020 134

Nitrogen, Total,HR HS-TN-H(CA) Chromatropic acid 10~100 2021 139

Orthophosphate,LR HS-PO4(P)-L Ascorbic acid 0.01~3 3020 144

Orthophosphate,HR HS-PO4(P)-H Molybdo vanadate 1~15 3030 147

Phenol HS-Phenol Direct Photometric 0.1~10 4500 150

Phosphorus, Total,LR HS-TP-L Ascorbic acid 0.01~3 3000 153

Phosphorus, Total,HR HS-TP-H Molybdo vanadate 1~15 3010 158

Silica-L HS-Si-L Molydosilicate 0.5~20 5500 163

Silica-H HS-SI-H Molybdosilicate 20~100 5510 166

Sulfate,LR HS-SO4-L Gravimetric 5~50 4600 169

Sulfate,HR HS-SO4-H Gravimetric 10~70 4601 172

Sulfide HS-S Methylene blue 0.5~5 5400 175

Turbidity HS-Turb. Absorption 0~400FTU 6700 179

Zinc HS-Zn Zincon 0.1~5 4200 180

■ CATEGORIES



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Aluminium

Method 5000ECR Method

Al(0.01 ~ 0.3mg/L)Scope and Application : water and wastewater

Al (0.01 ~ 0.3mg/L)

1) Add 5ml of distilled water to a sample cell (round glass vial) to prepare reagent blank test.

2) Add 5ml of sample to another sample cell (round glass vial) to be tested using a precise pipet.

3) Add 2ml of S1 to each cell. Cap tightly and shake it up and down slowly for 10 times to mix them.

4) Add one of Powder Pillow(P) to each cell, cap tightly and shake them up and down slowly for 10 times to dissolve the P.

5) Add 1ml of S2 to each cell.



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Aluminium

Aluminium

5) Add five drops of S3 to the only reagent blank cell, contained distilled water. Cap tightly and shake them up and down for 10 times.

6) After 5 minutes, wipe the cell and place it into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.



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Aluminium

Aluminium

InterferencesIf sample to be tested is colorful, other than transparent, it can affect to the actual data.

Floating substance in the sample works as an interference to the test. For the best result, reading of the result should be performed within 5minutes after testing. If fluoride, polyphosphate and alkalinity in the sample, they work as an interference. If there is some sulfate in the sample, the concentration is lower than 2,000 ㎎/L, it does not work as an interference Sample Collection and Preservation

Collect samples using an plastic container and analyze it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated nitric acid to pH of 2~5 (about 1.5㎖ per liter). It can be stored for 6 months at room temperature. Before, analyzing, adjust pH to neutralization with sodium hydroxide solution(5N). In case of that sample contains suspended substances, separate the substance from the samples. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of nitric acid and 4ml of hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L

Accuracy and PrecisionWhen 0.08㎎/L of standard solution is investigated, check it the difference from the

actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.

Standard Solution Make a standard solution to check accuracy and calibration by commercial standard solution, 1,000mg/L.

Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.01㎎/L, so test should be performed within appropriate concentration range.



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Aluminium

Item Test No. Cat. No.

HS-Al Set 100/set 27010

Aluminium

Contents

Description Quantity Requiredper test Unit Cat. No.

Al Powder Pillow (P) 1~2 100Ea/set 27010-10

Al Solution - 1 (S1) - 1bottle/set 27010-20





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Aluminium

Cadmium

Method 6000Dithizone

Cd(0.01 ~ 0.5mg/L)Scope and Application : water and wastewater

Cd (0.01 ~ 0.5mg/L)

1) Add 5ml of distilled water to sample cell (round glass vial) to prepare blank test.


3) Add 1ml of S1 to each sample cell, cap tightly and shake it up and down slowly for 10 times to mix them.

4) Add 0.2ml of S2 to each sample cell, cap tightly and shake it up and down slowly for 10 times to mix them.

5) Add one of powder pillow(P) to each sample cell and shake vigorously until the reagent is completely dissolve.



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Cadmium

Cadmium

6) Wait 2 minutes for maximum color development. At end of 2 minute waiting, clean and insert the vial into cell holder to read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual .



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Cadmium

Cadmium


Floating substance in the sample works as an interference to the test. It is necessary to filtering for the best result. A strong oxidizing and reducing agent in the sample will interfere. The substance in the below table lie below the limit at which the determination is interfered with.

Substance Conc.(mg/L) Substance Conc.(mg/L) or %

Aluminum 25 Magnesium 1000

Calcium 1000 Ammonium 1000

Lead 100 Nickel 0.01

Cobalt 1 Nitrite 0.1

Chromium(Cr2O7) 100 Phosphorous 100

Copper 10 Zinc 1

Manganese 50 Na2SO4 1%

NH4+ 100 NaNO3 10%

Iron(3+) 1 NaCl 1%

Sample Collection and PreservationCollect samples using an acid-cleaned glass and plastic container and analyze it

promptly for good result. It can preserve with nitric acid 65%(1ml/1L sample)



Standard Solution Dilute commercial cadmium standard solution containing 0.1, 0.25 and 0.5 mg/L.




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HS-Cd Set 25/set 41010

Cadmium

Cadmium

Contents


Cd Solution(S1) - 1bottle/set 41010-20

Cd Solution(S2) - 1bottle/set 41010-21

Cd Powder Pillow(P) 1~2 25Ea/set 41010-10



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Cadmium

Chloride

Method 5100Mercuric Thiocyanate Method

Cl-L(1 ~ 20mg/L)Scope and Application : water and wastewater

Cl-L (1 ~ 20mg/L) 1) Add 2.5ml of distilled water to sample cell(round glass vial) to prepare reagent blank test.

2) Add 2.5ml of sample to another sample cell(round glass vial) to be tested using a precise pipet.

3) Add 5ml of S-1 to each kit, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix them.

4) Add 0.5ml of S-2 to each kit, which is distilled water and sample add. Cap



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tightly and shake it up and down slowly for 10 times to mix it.

5) Add 1ml of S-3 to each kit, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.

6) Wait 5 minutes for maximum color development. At end of 5 minute waiting, insert the vial into cell holder to read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.

Chloride

Chloride

InterferencesIf sample to be tested is colorful, other than transparent, it can

affect to the actual data. Floating substance in the sample works as an interference to the test. It is necessary to filtering for the best result. A strong oxidizing and reducing agent in the sample will interfere.

Sample Collection and PreservationCollect samples using an acid-cleaned glass and plastic

container and analyze it promptly for good result. It can be stored for 28 days at room temperature.

Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from the




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Chloride


HS-Cl-L Set 100/set 35012

Standard Solution Dissolve 0.261g of NaCl(Sodium Chloride) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.

Detection Limit Errors are more frequent if the concentration of a sample is lower than 1㎎/L, so test should be performed within appropriate concentration range.

Chloride

Contents


Cl-L Solution(S-1) - 1bottle/set 35012-20





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Chloride

Chloride

Method 5101Mercuric Thiocyanate Method

Cl-H(20 ~ 60mg/L)Scope and Application : water and wastewater

Cl-H (20 ~ 60mg/L)

1) Add 2.5ml of distilled water to sample cell(round glass vial) to prepare reagent blank test.

2) Add 2.5ml of sample to another sample cell(round glass vial) to be tested



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using a precise pipet.

3) Add 5ml of S-1 to each kit, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix them.

4) Add 0.5ml of S-2 to each kit, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.

5) Add 1ml of S-3 to each kit, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.

6) Wait 5 minutes for maximum color development. At end of 5 minute waiting, insert the vial into cell holder to read the result. Please refer to chapter 4. HUMAS Program of

Instrument Manual for test procedure.

Chloride

Chloride




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Chloride

Floating substance in the sample works as an interference to the test. It is necessary to filtering for the best result. A strong oxidizing and reducing agent in the sample will interfere.


promptly for good result. It can be stored for 28 days at room temperature.



Standard Solution Dissolve 0.261g of NaCl(Sodium Chloride) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.


Chloride

Contents



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HS-Cl-H Set 100/set 35014


Cl-H Solution(S-1) - 1bottle/set 35014-20



Chloride

Free Chlorine

Method 4310DPD Method

Cl2(Free)(0.1 ~ 2mg/L)



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Scope and Application : water and wastewater

Cl2(Free) (0.1 ~ 2mg/L)

1) Add 10ml of distilled water to sample cell(round glass vial) to prepare reagent blank test.

2) Add 10ml of sample to another sample cell(round glass vial) to be tested

using a precise pipet.

3) Add one of Powder Pillow(P) to the only sample cell, contained sample to be tested, cap and shake it vigorously to dissolve the reagent for 15 seconds.

4) After 2 minutes, wipe the cells and place it into cell holder and read the result. Please refer to chapter 4.

HUMAS Program of Instrument Manual for test procedure.

Free Chlorine

Free Chlorine

Interferences



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Free Chlorine

1) Oxidizer, Br2, ClO2, I2 and O3 in the sample will interfere to the test.2) High buffered sample is need to adjust pH to 6~7.3) When oxidized manganese(Mn4+, Mn7+) presents in the sample, perform following.

① Adjust pH to between 6~7. ② Add 3 drops of potassium iodide (30g/L). ③ Mix it and leave for one minute. ④ Add 3 drops of sodium arsenite(5/L). ⑤ Test the sample treated by above procedure. ⑥ For the best result, subtract the value measured by above treatment from

the result measured original testing procedure. Sample collection and Preservation

Rinse thoroughly the container(avoid plastic bottle) with deionized or distilled water. Analyze the sample immediately after collection because free chlorine is strong oxidizing agent and it is unstable in natural water. Do not use the same sample for testing of free and total chlorine. When sampling from a tap water, let the water flow for at least 5 minutes to ensure a representative sample. And don't make a bubble in the sample.



Standard Solution Dissolve 0.891g of KMnO4(Potassium Permanganate) in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.

Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎,so test should be performed within appropriate concentration range.

Free Chlorine

Contents



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HS-Cl2(Free) Set 100/set 10030


Cl2(Free) Powder Pillow(P) 1~2 100Ea/set 10030-10

Free Chlorine

Chlorine, Total

Method 4300



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DPD MethodCl2(T)(0.1 ~ 2mg/L)


Cl2(T) (0.1 ~ 2mg/L)


2) Add 10ml of sample to another sample cell(round glass vial) to be tested using a precise pipet.

3) Add one of Powder Pillow(P) to the only sample cell,

contained sample to be tested, cap and shake it vigorously to dissolve the reagent for 15 seconds.

4) After 2 minutes, wipe the cells and place it into cell

holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.

Chlorine, Total

Chlorine, Total



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Chlorine, Total

Interferences1) Oxidizer, Br2, ClO2, I2 and O3 in the sample will interfere to the test.2) High buffered sample is need to adjust pH to 6~7.3) When oxidized manganese(Mn4+, Mn7+) presents in the sample, perform following.

① Adjust pH to between 6~7. ② Add 3 drops of potassium iodide (30g/L). ③ Mix it and leave for one minute. ④ Add 3 drops of sodium arsenite(5/L). ⑤ Test the sample treated by above procedure. ⑥ For the best result, subtract the value measured by above treatment from

the result measured original testing procedure. Sample collection and Preservation

Rinse thoroughly the container(avoid plastic bottle) with deionized or distilled water. Analyze the sample immediately after collection because free chlorine is strong oxidizing agent and it is unstable in natural water. Do not use the same sample for testing of free and total chlorine. When sampling from a tap water, let the water flow for at least 5 minutes to ensure a representative sample. And don't make a bubble in the sample.



Standard Solution Dissolve 0.891g of KMnO4(Potassium Permanganate) in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.

Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎,so test should be performed within appropriate concentration range.

Chlorine, Total



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HS-Cl2(T) Set 100/set 10020

Contents


Cl2(T) Powder Pillow(P) 1~2 100Ea/set 10020-10

Chlorine, Total



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Chromium, Hexavalent

Method 4410Diphenylcarbazide Method

Cr(+6)(0.1 ~ 1mg/L)Scope and Application : water and wastewater

Cr(+6) (0.1 ~ 1mg/L)


] 2) Add 10ml of sample to another sample cell(round glass vial) to be tested using a precise pipet.

3) Add Powder Pillow(P) to each cell, cap and shake them intensively for 15 seconds to mix them.

4) After 15 minute reaction, wipe the cells and place them into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test.




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Interferences1) High buffered sample is need to adjust pH to 6~7.2) If samples contain greater than 1mg/L Iron, it may will interfere.

3) If samples contain greater than 1mg/L Vanadium, it may will interfere.

Sample Collection and Preservation Collect samples using an acid-cleaned glass and plastic container and analyze it promptly for good result. It can be stored for 24 Hrs at 4℃ and it should be analyzed within 24 Hrs.



Standard Solution Dissolve 0.283g of K2Cr2O7(Potassium Dichromate) which has been dried for more than 3 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution. Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.



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HS-Cr(+6) Set 100/set 11010


Contents


Cr(+6) Powder Pillow(P) 1~2 100/set 11010-10




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Chromium, Total

Method 4400Diphenylcarbazide Method

Cr(T)(0.1 ~ 1mg/L)Scope and Application : water and wastewater

Cr(T) (0.1 ~ 1mg/L)



3) Add one of Powder Pillow-1(P1) to two sample cells respectively.

4) Add one of Powder Pillow-2(P2) to each sample cell, and cap the vials tightly and shake the cells vigorously for 30 seconds to mix them.

5) Place the vials in the reactor(HS-R 200) for 25 minutes at 100℃. (Make the reactor's temperature to 100℃ in advance)

6) After times over, cool the sample cells to room temperature with cold water or tap water.

7) Add one of Powder Pillow-3(P3) to each cell, and shake the vials up and down intensively for 30 seconds to mix them.

8) Add one of Powder Pillow-4(P4) to each cell, and shake the vials up and down intensively for 30 seconds to mix them. 9) Add one of Powder Pillow-5(P5) to each cell, and shake

the vials up and down intensively for 30 seconds to mix them.

Chromium, Total



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Chromium, Total

10) Wait 15 minutes for reaction.

11) Wipe the cells and place vials into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual.

Chromium, Total



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Chromium, Total

Chromium, Total

Interferences1) High buffered sample is need to adjust pH to 6~7.2) Large amount of organic materials in the samples will interfere to completed oxidize

Chromium(3+). Therefore, it should be digested before testing for the best result.

Sample Collection and Preservation Collect samples using an acid-cleaned glass and plastic container and analyze it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated nitric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 6 months at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N). ※ Compensation as adding the reagent Ex.) When sample which has 2ml of nitric acid sodium hydroxide add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L



Standard Solution Dissolve 0.283g of K2Cr2O7(Potassium Dichromate) which has been dried for more than 3 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution. Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.



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HS-Cr(T) Set 100/set 12010

Chromium, Total

Contents


Cr(T) Powder Pillow-1(P1) 1~2 100Ea/set 12010-10





Chromium, Total



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Cobalt

Method 6400PAN Method

Co(0.05 ~ 2mg/L)Scope and Application : water and wastewater

Cobalt (0.05 ~ 2mg/L)

1) Add 10ml of distilled water to a sample cell(round glass vial) to prepare reagent blank test.


3) Add one of Powder Pillow-1(P1) to each cell, which is distilled water and sample add. Cap tightly and shake it vigorously to dissolve it.

4) Add 0.5ml of S to each cell. Cap tightly and shake it up and down slowly for 10 times to mix it.

5) Wait 15minutes for reaction.

Cobalt



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Cobalt

6) Add one of Powder Pillow-2(P2) to each cell, which is distilled water and sample add. Cap tightly and shake It gently to dissolve it.

7) After 1minute reaction, wipe the cells and place vials into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual.

Cobalt



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Cobalt

Interferences


Al 60 K 500

Ca 1000(as CaCO3) Mg 400

Cd 30 Mn 45

Cl 8000 Mo 60

Complex reagents All range Na 5000

Cr(+3) 20 Pb 30

Cr(+6) 80 Zn 40

Cu 15 Buffer reagents Pre-treatment

F(-) 20

Ni All rangeFe(+2) All range

Fe(+3) 30

Sample Collection and Preservation

Take samples using a plastic container cleaned with acids and analyze it promptly for good result. If prompt analysis is not possible, add 5㎖ of concentrated nitric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 6 months at room temperature. Before analyzing, adjust pH of sample to neutral range. When pH of sample is higher than 8, sediments will be produced in the sample which work as a interfere. ※ Compensation as adding the reagent Ex.) When sample which has 5ml of nitric acid and sodium hydroxide add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1000㎖ + 5㎖ + 4 = 1009㎖○ Calculating compensation value = 1009/1000 =1.009○ Actual Concentration = 10㎎/L × 1.009 = 10.09㎎/L



Standard SolutionA commercial standard solution(1000㎖/l) is recommend to dilute proper

concentration.

Detection LimitErrors are more frequent if the concentration of a sample is lower than 0.05㎎/L.

Cobalt



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HS-Co Set 100/set 43010

Cobalt

Contents


Co Powder Pillow-1(P1) 1~2 100Ea/set 43010-10

Co Powder Pillow-2(P2) 1~2 100Ea/set 43010-11

Co Solution(S) - 1bottle/set 43010-20

Cobalt



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Chemical Oxygen Demand(CODCr)

Method 1000Reactor Digestion Method

COD-U(5 ~ 40mg/L)Scope and Application : water and wastewater

COD-UR (5 ~ 40mg/L)

1) To prepare the reagent blank test, add 2㎖ of distilled water to COD-U Kit.

2) Take 2㎖ of a sample to be tested using a precise pipet and add to different COD-U Kit.

3) Close it with cap and shake it up and down slowly for 10 times to mix it.

4) Put the kits to reactor(HS-R-200) and heat it for 2 hours at 150℃ for pre-treatment. Place the plastic cover on the reactor. (Make the reactor's temperature to 150℃ in advance)




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5) After heating reaction, take the kits from the reactor and leave it on for 10 minutes at room temperature. Then, shake it up and down softly for 10 times to mix it. And cool the vials to room temperature, clean outside of the vials.

6) See Chapter 4. HUMAS Program of the Instrument Manual.



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InterferencesIf there is some chloride(Cl-) in the sample, the measured concentration can be higher

than the actual concentration. The concentration is lower than 2,500㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride.

Sample Collection and PreservationTake samples using a glass container and analyze it promptly for good result. If

prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 30㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1000㎖ + 2㎖ = 1002㎖○ Calculating compensation value = 1002/1000 =1.002○ Actual Concentration = 30㎎/L × 1.002 = 30.06㎎/L



Standard SolutionGrind Potassium hydrogen phthalate(KHP) in fine size and dry it for 2 hours at 110℃.

Then, the dried KHP of 0.425g is dissolved in distilled water of 1000㎖. It becomes standard solution of COD 500㎎/L and refrigerated at 4℃.

Detection LimitErrors are more frequent if the concentration of a sample is lower than 5㎎/L.



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HS-COD-UR Set 25/set 01011


Contents


COD-UR Kit 1~2 25Ea/box 01011-01




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COD-L(15 ~ 150mg/L)Scope and Application : water and wastewater

COD-L (15 ~ 150mg/L)

1) To prepare the reagent blank test, add 2㎖ of distilled water to COD-L Kit.

2) Take 2㎖ of a sample to be tested using a precise pipet and add to different COD-L Kit.


4) Put the kits to reactor(HS-R 200) and heat it for 2 hours at 150℃ for pre-treatment. Place the plastic cover on the reactor. (Make the reactor's temperature to 150℃ in advance)




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6) See Chapter 4. HUMAS Program of the Instrument Manual and measure the sample.



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than the actual concentration. The concentration is lower than 3,000㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride.


prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 100㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1000㎖ + 2㎖ = 1002㎖○ Calculating compensation value = 1002/1000 =1.002○ Actual Concentration = 100㎎/L × 1.002 = 100.2㎎/L



Standard SolutionGrind Potassium hydrogen phthalate(KHP) in fine size and dry it for 2 hours at 110℃.

Then, the dried KHP of 0.425g is dissolved in distilled water of 1000㎖. It becomes standard solution of COD 500㎎/L and refrigerated at 4℃.

Detection LimitErrors are more frequent if the concentration of a sample is lower than 15㎎/L.



- 41 -


HS-COD-LR Set 25/set 01012


Contents


COD-LR Kit 1~2 25Ea/set 01012-01




- 42 -



COD-M(50 ~ 1,500mg/L), COD-H(500~15,000mg/L)Scope and Application : water and wastewater

COD-M (50 ~ 1,500mg/L), COD-H (500 ~ 15,000mg/L)

1) To prepare the reagent blank test, add 2㎖ of distilled water to COD-M Kit. (In case of COD-H, add 0.2mL).

2) Take 2㎖ of a sample to be tested using a precise pipet and add to different COD-M Kit. (In case of COD-H, add 0.2mL).


4) Put the kits to reactor(HS-R 200) and heat it for 2 hours at 150℃ for pre-treatment. Place the plastic cover on the reactor. (Make the reactor's temperature to 150℃ in advance)




- 43 -




6) See Chapter 4. HUMAS Program of the Instrument Manual and measure the samples. ※When using COD-H Kit, measure the sample by COD-M Program and multiply the value by 10.



- 44 -




than the actual concentration. In case of COD-M, Cl- is under 3,000㎎/L, it does not work as an interference. n case of COD-H, Cl- is under 40,000㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride.


prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 1000㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ = 1,002㎖○ Calculating compensation value = 1,002/1,000 =1.002○ Actual Concentration = 1,000㎎/L × 1.002 = 1,002㎎/L



Standard SolutionGrind Potassium hydrogen phthalate(KHP) in fine size and dry it for 2 hours. Then,

the dried KHP of 0.425g is dissolved in distilled water of 1000㎖. It becomes standard solution of COD 500㎎/L and refrigerated at 4℃.

Detection LimitCOO-M : Errors are more frequent if the concentration of a sample is lower

than 50㎎/L.COD-H : Errors are more frequent if the concentration of a sample is lower

than 500㎎/L.



- 45 -


HS-COD-M Set 25/set 01013

HS-COD-H Set 25/set 01014


Contents


COD-M Kit (50 ~ 1,500mg/L) 1~2 25Ea/set 01013-01

COD-H Kit (500 ~ 15,000mg/L) 1~2 25Ea/set 01014-01




- 46 -

Chemical Oxygen Demand(CODMn)


COD(Mn)-L(0.2 ~ 3mg/L)Scope and Application : water and wastewater

COD(Mn)-U (0.2 ~ 3mg/L)

1) To prepare the reagent blank test, add 5㎖ of distilled water to COD-Mn-U Kit.

2) Take 5㎖ of sample to be tested using a precise pipette and add to different COD-Mn-U Kit.

3) Add 0.6ml of S to each COD-Mn-U Kit(vial) using a precise pipet.





- 47 -



5) Put the kit to reactor(HS-R 200) and heat it for 15 minutes at 100℃. Place the plastic cover on the reactor. (Make the reactor's temperature to 100℃ in advance)

6) After heating reaction, take the kits from the reactor and cool it down to room temperature using cold water such as tap water. And clean outside of the vials. (After cooling, it should be measured within 3 minutes. It is very important to the best results)

7) See Chapter 4. HUMAS Program of the Instrument Manual and measure the samples.



- 48 -



Interferences

Substance Conc.(mg/L)

Sulfide All range

Calcium 100

Magnesium 200

Free chlorine 3.5

Other oxidants Ozone, Hydrogen peroxide

Sample Collection and PreservationCollect samples using a glass container and analyze it promptly for good result. If

prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ = 1,002㎖○ Calculating compensation value = 1,002/1,000 =1.002○ Correct Concentration = 10㎎/L × 1.002 = 10.02㎎/L

Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from

the actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.

Standard SolutionGlucose(C6H12O6) in fine size and dry it for 1 hour. After cooling it to room

temperature in desiccator, the dried Glucose of 0.938g is dissolved in distilled water of 1000㎖. It becomes standard solution of COD 567㎎/L and refrigerated at 4℃.

Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.6㎎/L. So, test should be performed within appropriate concentration range.



- 49 -


HS-COD-Mn-U Set 25/set 01120



Contents


COD(Mn)-U Kit 1~2 25Ea/set 01120-01

COD(Mn)-U Solution(S) - 1bottle/set 01120-20



- 50 -


Method 1500Reactor Digestion MethodCOD(Mn)-L(0.6 ~ 20mg/L)


COD(Mn)-L (0.6 ~ 20mg/L)

1) To prepare the reagent blank test, add 3㎖ of distilled water to COD-Mn-L Kit.

2) Take 3㎖ of sample to be tested using a precise pipette and add to different COD-Mn-L Kit.

3) Add 0.6ml of S to each COD-Mn-L Kit(vial) using a precise pipet.





- 51 -





7) See Chapter 4. HUMAS Program of the Instrument Manual and measure the samples.



- 52 -




than the actual concentration. The concentration is lower than 200㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride.



Accuracy and PrecisionWhen 10㎎/L of standard solution is investigated, check it the difference from

the actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.






- 53 -


HS-COD-Mn-L Set 25/set 01110


Contents


COD(Mn)-L Kit 1~2 25Ea/set 01110-01

COD(Mn)-L Solution(S) - 1Bottle/set 01110-20




- 54 -



COD(Mn)-H(20 ~ 100mg/L)Scope and Application : water and wastewater

COD(Mn)-H (20 ~ 100mg/L)

1) To prepare the reagent blank test, add 0.5㎖ of distilled water to COD-Mn-H Kit.

2) Take 0.5㎖ of a sample to be tested using a precise pipet and add to different COD-Mn-H Kit.

3) Add 0.6ml of S to each COD-Mn-H Kit(vial) using a precise pipet.

4) Close it with cap and shake it up and down slowly for 10 imes to mix it.




- 55 -





7) See Chapter 4. HUMAS Program of the Instrument Manual.



- 56 -




than the actual concentration. The concentration is lower than 600㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride.










- 57 -


HS-COD-Mn-H Set 25/set 19014


Contents


COD(Mn)-H Kit 1~2 25Ea/set 19014-01

COD(Mn)-H Solution(S) - 1bottle/set 19014-20




- 58 -

Color

Method No. 6600Platinum Cobalt

Color (50~1000°)Scope and Application : water and wastewater

Color (50 ~ 1000°)



3) Wipe place the vials into the cell holder to read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.

Color



- 59 -

Copper

Method 4000Bathocuproine Method

Cu(0.1 ~ 5mg/L)Scope and Application : water and wastewater

Cu (0.1 ~ 5mg/L)



3) Add 0.3ml of S-1 to each sample cell, which is distilled water and sample add.Cap tightly and shake the vials up and down for 10 times to mix them.

4) Add 1ml of S-2 to each sample cell, which is distilled water and sample add. Cap tightly and shake the vials up and down for 10 times to mix them

5) Add 0.5ml of S-3 to each sample cell, which is distilled water and sample contained.

Copper



- 60 -

Copper

5) Cap the vials and gently invert the vials for 10 times.

6) After 10 minute reaction, wipe place the vials into the cell holder to read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual .

Copper



- 61 -

Copper

InterferencesThe following substances have been tested for interference and showed not work as

interference up to indicated levels.

Substance Conc.(mg/L) Substance Conc.(mg/L)

Al 100 Na 1000

Be 10 Zn 200

Cd 100 Cl- 1000

Ca 1000 F- 500

Cr(+6) 10 NO3 200

Cr(+3) 5 NO2 200

Iron(+2) 100 PO4 1000

Iron(+3) 100 SO4 1000

Li 500 Cl2 1

Ni 100 pH 4 ~ 7

Sample Collection and PreservationCollect samples using a glass and plastic container and analyze it promptly for good

result. If prompt analysis is not possible, add 2㎖ of concentrated nitric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N). In case of that pH of sample is higher than 6, copper will precipitate.※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L Accuracy and Precision

When 1㎎/L of standard solution is investigated, check it the difference from the actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.

Standard Solution Dissolve 0.393g of CuSO4․5H2O(Cuperic Sulfate Pentahydrate) in 20ml of nitric acid(1+1), and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.


Copper



- 62 -

Copper


HS-Cu Set 100/set 15010

Copper

Contents


Cu Solution-1 (S1) - 1bottle/set 15010-20





- 63 -

Cyanide

Method 4900DP Method

CN(0.005 ~ 0.5mg/L)Scope and Application : water and wastewater

CN (0.005 ~ 0.5mg/L)

1) Add 10ml of distilled water to CN Kit to prepare reagent blank test.

2) Add 10ml of sample to CN Kit to be tested using a precise pipet.

3) Add one of Powder Pillow-1(P1) to each kit, which is distilled water and sample add. Cap tightly and shake them vigorously to dissolve the reagent.

4) Place the vials in the reactor(HS-R 200) for 30 minutes at 120℃. (Make the reactor's temperature to 120℃ in advance).

Place the plastic cover on the reactor. After heating reaction, remove the kits from the reactor and cool it to room temperature.

Cyanide



- 64 -

Cyanide

Cyanide

5) Add 4 drops of S to each vial, and shake them up and down slowly for 10 times to mix them.

6) Add one of Powder Pillow-2(P2) to each kit, which is distilled water and sample add. Cap tightly and shake them vigorously to dissolve the reagent.

7) Add one of Powder Pillow-3(P3) to each kit, which is distilled water and sample add. Cap tightly and shake them vigorously to mix them.

8) Take 10 minute reaction, wipe kits and place it into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.



- 65 -

Cyanide

Cyanide

Interferences


Silver 10 Mercury 0.1

Aluminum 100 Magnesium 1000

Bromine 0.1 Manganese 1000

Calcium 1000 Ammonium 1000

Cadmium 100 Nickel 0.01

Cobalt 1 Nitrite 10

Chromium(3+) 100 Phosphorous 1000

Copper 5 Sulfate 5

Fluorine 1000 Zinc 100

Iron(2+) 50 EDTA 1%

Iron(3+) 100 NaCl 10%


Collect samples using an acid-cleaned glass and plastic container and analyze it promptly for good result. If prompt analysis is not possible, add 4㎖ of sodium hydroxide solution(5N) to pH of higher than 12. It can be stored for 14days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with acid solution. In case of that sample contains sulfate, test the sample within 24 hours. ※ Compensation as adding the reagent Ex.) When sample which has 4ml of sodium hydroxide solution and 2ml of acid solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L



Standard Solution Dissolve 2.51g of KCN(Potassium Cyanide) in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.




- 66 -


HS-CN Set 25/set 25010

Cyanide

Contents


CN Kit 1~2 25Ea/set 25010-01

CN Powder Pillow-1(P1) 1~2 25Ea/set 25010-10



CN Solution(S) - 1bottle/set 25010-20

Cyanide



- 67 -

Detergent

Method 6200Crystal violet MethodABS(0.01 ~ 0.3mg/L)


ABS (0.01 ~ 0.3mg/L)

1) Fill a clean 250ml separatory funnel to the 200ml mark with sample.

2) Add 5ml of S-1 to the separatory funnel to using a precise pipet. And shake slowly for 5 seconds. Be careful not over flow, it can effect the result. Harmful to skin and cloth.

3) Add one of Powder Pillow(P) to the funnel, and cap tightly and shake slowly to dissolve the reagent completely.

4) Add 20ml of S-2 to the separatory funnel to using a precise pipet. And shake slowly for 1 minutes. And wait 30 minutes for the reaction.

Detergent



- 68 -

Detergent

5) And wait 30 minutes for the reaction.

6) After 30 minutes, drain the bottom layer of the funnel. It should be treated by regulated method.

7) Drain the top layer of the funnel to a sample cell, and cap and wipe for measuring. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure. To prepare blank cell, add 5㎖ of concentrated benzene to another sample cell.

Detergent



- 69 -

Detergent

Interferences A large amount chloride will interfere to the test.

Sample Collection and PreservationCollect samples using an plastic or glass container and analyze it promptly for good

result. If prompt analysis is not possible, it can be stored for 24 hours at 4℃. Before, analyzing, warm sample to room temperature.


actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean. Standard Solution To check accuracy of this instrument, use a commercial standard solution. And dilute the standard solution within proper concentration. Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.001㎎/L, so test should be performed within appropriate concentration range.

Detergent



- 70 -


HS-ABS Set 100/set 40010

Detergent

Contents


ABS Solution(S-1) - 1bottle/set 40010-20

ABS Solution(S-2) - 1bottle/set 40010-21

ABS Powder Pillow-1(P1) 1~2 100Ea/set 40010-10

Detergent



- 71 -

Fluoride

Method 4700SPANDS Method

F-(0.1 ~ 1.5mg/L)Scope and Application : water and wastewater

F- (0.1 ~ 1.5mg/L)



3) Add 1ml of S to each cell, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix them.

4) After 1 minute reaction, wipe the cells and place it into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual.

Fluoride



- 72 -

Fluoride

Fluoride

Interferences

Substance Conc. (mg/L)

Alkalinity 5000mg/L(as CaCO3)

Aluminum 0.1mg/L

Chloride 7000mg/L(as Cl-)

Chorine 5mg/L(as Cl2)

Ferrous 10mg/L(as Iron, ferric)

Orthophosphate 200mg/L(as PO4-3)


Collect samples using an acid-cleaned plastic container and analyze it promptly for good result. If prompt analysis is not possible, it can be stored for 7days at 4℃. Before, analyzing, warm sample to room temperature.


actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean. Standard Solution Dissolve 2.21g of NaF(Sodium Fluoride) which has been heated for 50 minutes at 550 ℃ and cooled in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution. Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.



- 73 -


HS-F- Set 100/set 26010

Fluoride

Contents


F- Solution(S) - 1bottle/box 26010-10

Fluoride



- 74 -

Hardness

Hardness

Method No. 7000(Ca Hardness), 7001(Mg Hardness)Calmagite Method

Hardness(0.1 ~ 4mg/L)Scope and Application : water and wastewater

Hardness (0.1 ~ 4mg/L)

1) Pour the 100ml sample which should be diluted as the

following example, into graduated cylinder.

※ Example of dilution

① Soften water (0~75 mg/L) : Dilute20 times with distilled water

② Light soften water (75~150 mg/L) : Dilute 50 times with distilled water ③ Hard water (150~350 mg/L) : Dilute 100 times with distilled water

2) Add 1ml of S-1 to the sample. Cap tightly and shake it

gently for 10 times.

3) Add 2ml of S-2 to the sample. Cap tightly and shake it

gently to mix well for 10 times.



- 75 -

Hardness

Hardness(Mg+2&Ca+2)

4) Pour 10 ml of the sample added reagents into each of

three sample cells. (First, Second and Third)

5) Add one drop of S-3 to the First cell, and shake gently up

and down for 10 times. (For the blank test)

6) Add one drop of S4 to Second cell, and shake up and

down for 10 times to mix it.

7) Select category Mg+2(HD). To set zero base, insert the First

cell added S3 into cell holder and press zero button.

8) Place the Second cell added S3 into cell holder, and read the result of Mg+2(HD).

(Record the data)

9) After measurement of Mg+2(HD), don't remove the Second cell to set zero base.

Select category Ca+2(HD) and do zero base.

10) Place the Third cell into cell holder, and read the result of Ca+2(HD). (Record the

data)

11) Total Hardness = Mg+2(HD) + Ca+2(HD)



- 76 -

Hardness

Hardness(Mg+2&Ca+2)

Interferences


Chromium(+3) 0.25mg/L

Copper 0.75mg/L

EDTA, EGTA 0.2mg/L(as CaCO3-)

Iron(+2) 1.4mg/L

Iron(+3) 2.0mg/L

Manganese(+2) 0.2mg/L

Zinc(+2) 0.05

Calcium>1.0 mg/LMagnesium>0.25 mg/L

For the best result, if Calcium is over 1.0 and Magnesium is over 0.25mg./L, re-test the sample.


Collect samples using an acid-cleaned plastic container and analyze it promptly for good result. If prompt analysis is not possible, it can be stored for 7days at 4℃ after adding 5ml nitric acid to adjust pH 2. Before, analyzing, warm sample to room temperature.


actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean. Standard Solution Dissolve 2.775g of MgCl2(Sodium Magnesium) in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution. Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎/L, so test should be performed within appropriate concentration range.



- 77 -


Hardness Set 100/set 24020

Hardness

Hardness(Mg+2&Ca+2)

Contents


Hardness S1 - 1bottle/set 24020-20






- 78 -

Hydrogen peroxide

Method 5300

Titanium sulfate Method

H2O2-L(0.5 ~ 30mg/L)


H2O2-L (0.5 ~ 30mg/L)

1) Add 5ml of distilled water to sample cell(round glass vial)

to prepare reagent blank test.

2) Add 5ml of sample to another sample cell(round glass

vial) to be tested using a precise pipet.

3) Add 0.5ml of S-1 to each cell, which is distilled water

and sample add. Cap tightly and shake it up and down

slowly for 10 times to mix it.

4) Add 1ml of S-2 to each cell, which is distilled water and

sample add. Cap tightly and shake it up and down slowly

for 10 times to mix it.

5) Wait 1 minutes for maximum color development. At end

of 2 minute waiting, insert the vial into cell holder to read

the result. Please refer to chapter 4. HUMAS Program of


Hydrogen peroxide



- 79 -

Hydrogen peroxide

Hydrogen peroxide

Interferences If sample to be tested is colorful, other than transparent, it can affect to the actual data.

Floating substance in the sample works as an interference to the test.

Sample Collection and Preservation Collect samples using an acid-cleaned glass and plastic container and analyze it

promptly for good result.


actual concentration of the standard solution is within 5%. Analyze the same sample more

than 7 times and get a standard deviation and arithmetic mean.

Standard SolutionTo make standard solution, dilute to 1,000mg/L using a commercial Hydrogen

peroxide.

Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.5㎎/L, so

test should be performed within appropriate concentration range.



- 80 -


H2O2-L Set 100/set 24020

Hydrogen peroxide

Contents

DescriptionQuantity Required

per test(vials)Unit Cat. No.

H2O2-L Solution(S-1) - 1bottle/set 26012-20

H2O2-L Solution(S-2) - 1bottle/set 26012-21

Hydrogen peroxide



- 81 -

Hydrogen peroxide

Method 5301

Titanium sulfate Method

H2O2-H(3 ~ 300mg/L)


H2O2-H (3 ~ 300mg/L)












of 2 minute waiting, insert the vial into cell holder to read



Hydrogen peroxide



- 82 -

Hydrogen peroxide

Hydrogen peroxide


Floating substance in the sample works as an interference to the test.






Standard SolutionTo make standard solution, dilute to 1,000mg/L using a commercial Hydrogen

peroxide.

Detection Limit Errors are more frequent if the concentration of a sample is lower than 3㎎/L, so




- 83 -


H2O2-H Set 100/set 26014

Hydrogen peroxide

Hydrogen peroxide

Contents



H2O2-H Solution(S-1) - 1bottle/set 26014-20

H2O2-H Solution(S-1) - 1bottle/set 26014-21



- 84 -

Iodine

Method 5200DPD Method

I2(0.1 ~ 2mg/L)


l2 (0.1 ~ 2mg/L)

1) Add 10ml of distilled water to sample cell (round glass

vial) to prepare reagent blank test.

2) Add 10ml of sample to another sample cell (round glass


3) Add one of Powder Pillow(P) to each kit, which is

distilled water and sample add. Cap tightly and shake the

vials vigorously for 15 seconds to mix them.


of 2 minute waiting, insert the vials into cell holder to read



Iodine



- 85 -

Iodine

Iodine

Interferences1) Oxidizer, Br2, ClO2, I2 and O3 in the sample will interfere to the test.

2) High buffered sample is need to adjust pH to 6~7.

3) When oxidized manganese(Mn4+, Mn7+) presents in the sample, perform following.

① Adjust pH to between 6~7.

② Add 3 drops of potassium iodide (30g/L).

③ Mix it and leave for one minute.

④ Add 3 drops of sodium arsenite(5/L).

⑤ Test the sample treated by above procedure.

⑥ For the best result, subtract the value measured by above treatment

from the result measured original testing procedure.

Sample collection and PreservationRinse thoroughly the container(avoid plastic bottle) with deionized or distilled water.

Analyze the sample immediately after collection because free chlorine is strong oxidizing agent

and it is unstable in natural water. Do not use the same sample for testing of free and total

chlorine. When sampling from a tap water, let the water flow for at least 5 minutes to

ensure a representative sample. And don't make a bubble in the sample.




Standard Solution Dissolve 0.891g of KMnO4(Potassium Permanganate) in distilled water, and make it 1L

precisely. It becomes 1,000㎎/ℓ of standard solution.

Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.1㎎,

so test should be performed within appropriate concentration range.



- 86 -

Iodine


l2 Set 100/set 36010

Iodine

Contents



l2 Powder Pillow(P) 1~2 100Ea/set 36010-10



- 87 -

Iron, Ferrous

Method 4110Phenanthroine Method

Fe(+2)(0.1 ~ 5mg/L)Scope and Application : water and wastewater

Fe(+2) (0.1 ~ 5mg/L)

1) Add 5㎖ of distilled water to sample cell(round glass vial) to prepare reagent blank test.

2) Add 5㎖ of sample to another sample cell (round glass vial) to be tested using a precise pipet.

3) Add 2ml of S to the each cell and shake gently up and down for 10 times to mix them.

4) Add one of Powder Pillow(P) to each kit, which is distilled water and sample add and release gas for 30 seconds. Cap tightly and shake it vigorously to dissolve the reagent.

5) Open the cap the release the CO2 gas from the vials, and take 10 minutes reaction times.

6) Wipe the vials and place it into cell holder and read the result. Please refer to chapter 4. Operation Guideline of Instrument Manual for test procedure.

Iron, Ferrous



- 88 -

Iron, Ferrous

Iron, Ferrous

InterferencesWhen the concentration of COD-cr, organic matters is higher than 500 ㎎/ℓ, test result is

higher than the actual one more than 5%. When the concentration of PO4-P is higher than 1mg/L, test result is higher than the actual one more than 5%. When the concentration of Cobalt is higher than 10mg/L, test result is lower than the actual one more than 5%. When the concentration of Cu is lower than 10mg/L, test result has an error within 1%.


Collect the sample using an container of glass or plastic acid-cleaned. It is recommended to perform the test promptly after sample is taken. Accuracy and Precision


Standard Solution Dissolve 7.02g of FeSO4(NH4)2SO4․6H2O(Ammonium Iron(Ⅱ) Sulfate Hexahydrate) in 20mL of Hydrochloric acid(1+1), and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.




- 89 -


HS-Fe(+2) Set 100/set 16020

Iron, Ferrous

Contents

Description Quantity Requiredper test(vials) Unit Cat. No.

Fe(+2) Solution (S) - 1bottle/set 16020-20

Fe(+2) Powder Pillow(P) 1~2 100Ea/set 16020-10

Iron, Ferrous



- 90 -

Iron, Total

Method 4100Phenanthroine Method

Fe(T)(0.1 ~ 5mg/L)Scope and Application : water and wastewater

Fe(T) (0.1 ~ 5mg/L)

1) Add 5ml of distilled water to a sample cell (round glass vial) to prepare reagent blank test.


3) Add one of Powder Pillow(P) and close them with cap, shake the vials up and down for 30 seconds gently to mix them.

4) Leave it for 5 minutes. 5) Wipe and place the cells into the cell holder within 5 minutes to read the results. Please refer to chapter 4. Operation Guideline of Instrument Manual for test .

Iron, Total



- 91 -

Iron, Total

Iron, Total

InterferencesIf sample to be tested is colorful, other than transparent, it can affect to the data as well as

floating substances. It should be filtered for the best result before testing. Also strong oxidizing and reducing agents in the sample can have negative affect to the data. Calcium is not effect at less than 10,000 mg/L as CaCO3 and Chloride is not effect at less than 180,000 mg/L. Magnesium is not work as an interference lower than 100,000mg/L.


Collect sample using a acid-cleaned glass or plastic container. It is recommended to perform the test promptly after sample is taken. If not possible, add 2mL of Conc. Nitric acid to it per one 1L of the sample to control the pH under 2. It can be preserved for more than 6 months at room temperature. Before testing, adjust the pH to between 3 and 5 with 5N sodium hydroxide solution. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of nitric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L Accuracy and Precision


Standard Solution Dissolve 7.02g of FeSO4(NH4)2SO4․6H2O(Ammonium Iron(Ⅱ) Sulfate Hexahydrate) in 20mL of Hydrochloric acid(1+1), and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.




- 92 -

Iron, Total


HS-Fe(T) Set 100/set 16010

Iron, Total

Contents


Fe(T) Powder Pillow-1 1~2 100Ea/set 16030-10



- 93 -

Manganese

Periodate oxidation Method

Mn(0 ~ 20mg/L)


Mn (0 ~ 20mg/L)


vial) to prepare reagent blank test.



3) Add Powder Pillow-1(P1) to the each cell, and shake them

intensively to dissolve completely the reagent. (About

30seconds)

4) Open them, add Powder Pillow-2(P2) to the each cell, and

shake them intensively to dissolve completely the reagent.

5) After 2 minute reaction, wipe the kits and place it into

cell holder and read the result. Please refer to chapter 4.

HUMAS Program of Instrument Manual for test procedure.

Manganese



- 94 -

Manganese

Manganese


Floating substance in the sample works as an interference to the test. It should be measured with

5 minute after testing, and the time is very important for the best results. If there are some

Calcium(Ca, 700mg/L), Chloride(Cl-, 70,000mg/L) and Magnesium(Mg, 100,00mg/L) in the

sample, the measured concentration can be higher than the actual concentration. The

concentration is lower than iron, 5㎎/L, it does not work as an interference.

Sample Collection and PreservationCollect samples using an acid-cleaned glass and avoid plastic container and analyze

it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated nitric

acid to pH of less than 2 (about 2㎖ per liter). It can be stored at 4℃. Before analyzing,

warm sample to room temperature and adjust pH to neutralization with sodium hydroxide

solution(5N). In case of that sample contains suspended substances, take the sample while

stirring to make the floating materials are even in it.

※ Compensation as adding the reagent Ex.) When sample which has 2ml of nitric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L




Standard Solution Make a standard solution to check accuracy and calibration by commercial standard

solution, 1,000mg/L.





- 95 -

Manganese


HS-Mn Set 100/set 13010

Manganese

Contents



Mn Powder Pillow-1(P1) - 100Ea/set 13010-10

Mn Powder Pillow-2(P2) - 100Ea/set 13010-11



- 96 -

Nickel

Method 6500PAN Method

Ni(0.05 ~ 2mg/L)Scope and Application : water and waste water

Nickel (0.05 ~ 2mg/L)



3) Add one of Powder Pillow-1(P1) to each cell, which is distilled water and sample add. Cap tightly and shake it vigorously to dissolve it.

4) Add 0.5ml of S to each cell. Cap tightly and shake it up and down slowly for 10 times to mix it.

5) Wait 15minutes for reaction.

Nickel



- 97 -

Nickel

6) Add one of Powder Pillow-2(P2) to each cell, which is distilled water and sample add. Cap tightly and shake It gently to dissolve it.

7) After 1minute reaction, wipe the cells and place vials into cell holder and read the result. Please refer to chapter 4. Operation Guideline of Instrument Manual for test procedure.

Nickel



- 98 -

Nickel

Interferences


Al 60 K 500

Ca 1,000(as CaCO3) Mg 400

Cd 30 Mn 45

Cl 8000 Mo 60

Complex reagents All range Na 5000

Cr(+3) 20 Pb 30

Cr(+6) 80 Zn 40

Cu 15 Buffer reagents Pre-treatment

F(-) 20

Co All rangeFe(+2) All range

Fe(+3) 30


Take samples using a plastic container cleaned with acids and analyze it promptly for good result. If prompt analysis is not possible, add 5㎖ of concentrated nitric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 6 months at room temperature. Before analyzing, adjust pH of sample to neutral range. ※ Compensation as adding the reagent Ex.) When sample which has 5ml of sulfuric acid add for preservation, is tested in 30㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1000㎖ + 5㎖ = 1005㎖○ Calculating compensation value = 1005/1000 =1.005○ Actual Concentration 30㎎/L × 1.005 = 29.85㎎/L



Standard SolutionA commercial standard solution(1000㎖/l) is recommend to dilute proper

concentration.

Detection LimitErrors are more frequent if the concentration of a sample is lower than 0.05㎎/L.

Nickel



- 99 -

Nickel


HS-Ni Set 100/set 42010

Nickel

Contents


Ni Powder Pillow-1(P1) 1~2 100Ea/set 42010-10

Ni Powder Pillow-2(P2) 1~2 100Ea/set 42010-11

Ni Solution(S) - 1bottle/set 42010-20



- 100 -

Nitrogen, Ammonia

Method 2800Indophenol Method

NH3(N)-U(0.03 ~ 1mg/L)Scope and Application : surface water and ground water

NH3(N)-U (0.03 ~ 1mg/L)

1) Add 10ml of distilled water to NH3(N)-U Kit to prepare reagent blank test.

2) Add 10ml of sample to NH3(N)-U Kit to be tested using a precise pipet.



Nitrogen, Ammonia



- 101 -

Nitrogen, Ammonia

5) Add Powder Pillow(P) and shake them up and down slowly for 10 times to mix them.

6) Place the kits in the reactor(HS-R 200) for 10 minutes at 100℃. (Make the reactor's temperature to 100℃ in advance)

7) After the time is over, remove the kits from the reactor, and cool it the room temperature with cold water such as tap water. (Cooling time : 90 seconds)

8) Wipe the kits and place it into cell holder and read the result. Please refer to chapter 4. Operation Guideline of Instrument Manual for test procedure.

Nitrogen, Ammonia



- 102 -

Nitrogen, Ammonia

Nitrogen, Ammonia


result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. If chlorine presents in the samples, add one drop of Sodium Thiosulfate(0.1N) for each 0.3mg/L Cl2 per 1L sample.※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L



Standard Solution Dissolve 0.3819g of NH4Cl(Ammonium Chloride) which has been dried for more than 5 hrs at 100 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.

Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.03 mg/L, so test should be performed within appropriate concentration range.



- 103 -


HS-NH3(N)-U Set 25/set 06031

Nitrogen, Ammonia

Contents


NH3(N)-U Kit 1~2 25Ea/set 06031-01

NH3(N)-U Powder Pillow(P) 1~2 25Ea/set 06031-10

NH3(N)-U Solution(S-1) - 1bottle/set 06031-20

NH3(N)-U Solution(S-2) - 1bottle/set 06031-21

Nitrogen, Ammonia



- 104 -

Nitrogen, Ammonia

Method 2810Nessler Method

NH3(NW)-L(0.2 ~ 6mg/L)Scope and Application : water and wastewater

NH3(N)-L (0.2 ~ 6mg/L)

1) Add 3ml of distilled water to sample cell (round glass vial) to prepare reagent blank test.

2) Add 3ml of sample to another sample cell (round glass vial)to be tested using a precise pipet.

3) Add 2ml of S1 to each cell.

4) Add 4 drops of S2 to each cell.

5) Cap the vials tightly, shake it up and down slowly for 10 times to mix them.

Nitrogen, Ammonia



- 105 -

Nitrogen, Ammonia

5) Open the cells and add 1ml of S3 to each cell and shake it up and down for 10 times.

6) Perform the test after 3minutes. Please refer to Chapter 4. Operation Guideline of Instrument Manual for test procedure.

Nitrogen, Ammonia



- 106 -

Nitrogen, Ammonia

Nitrogen, Ammonia


result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N approximately). In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. If chlorine presents in the samples, add one drop of Sodium Thiosulfate(0.1N) for each 0.3mg/L Cl2 per 1L sample. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L




Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.2 mg/L, so test should be performed within appropriate concentration range.



- 107 -


HS-NH3(N)-L Set 100/set 06022

Nitrogen, Ammonia

Contents


NH3(N)-L Solution(S-1) - 1bottle/set 06022-20



Nitrogen, Ammonia



- 108 -

Nitrogen, Ammonia

Method 2811Nessler Method

NH3(NW)-H(2 ~ 60mg/L)Scope and Application : water and wastewater

NH3(N)-H (2 ~ 60mg/L)

1) Add 0.5ml of distilled water to sample cell (round glass vial) to prepare reagent blank test.

2) Add 0.5ml of sample to another sample cell (round glass vial) to be tested using a precise pipet.

3) Add 6ml of S-1 to each sample cell.

4) Add 4 drops of S-2 to each sample cell.

4) Cap the vials tightly, shake it up and down slowly for 10 times to mix it.

Nitrogen, Ammonia



- 109 -

Nitrogen, Ammonia

5) Open it and add 1ml of S-3 to each sample cell and shake them up and down for 10 times.

6) After 3 minute reaction, wipe and place the cell into cell holder to read the result. Please refer to Chapter 4. Operation Guideline of Instrument Manual for test procedure.

Nitrogen, Ammonia



- 110 -

Nitrogen, Ammonia

Nitrogen, Ammonia


result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N approximately). In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. If chlorine presents in the samples, add one drop of Sodium Thiosulfate(0.1N) for each 0.3mg/L Cl2 per 1L sample. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L




Detection Limit Errors are more frequent if the concentration of a sample is lower than 2 mg/L, so test should be performed within appropriate concentration range.



- 111 -


HS-NH3(N)-H Set 100/set 06024

Nitrogen, Ammonia

Contents


NH3(N)-H Solution(S-1) - 1bottle/set 06024-20



Nitrogen, Ammonia



- 112 -

Nitrogen, Nitrate

Method 2200

Cadmium reduction Method

NO3(N)-L(0.2 ~ 5mg/L)


NO3(N)-L (0.2 ~ 5mg/L)




vial)to be tested using a precise pipet.

3) Add Powder Pillow(P) of one to the each sample cell,

Which is distilled water and sample add.

4) Shake them vigorously for one minute.

Nitrogen, Nitrate



- 113 -

Nitrogen, Nitrate

Nitrogen, Nitrate

5) Leave them for 5 minutes.

6) After reaction for 5 minutes, perform the test . Please

refer to Chapter 4. HUMAS Program for test procedure of

Instrument Manual.



- 114 -

Nitrogen, Nitrate

Nitrogen, Nitrate

Interferences

If sample to be tested is colorful, other than transparent, it can affect to the data as well as

floating matters. The analysis should be performed within 3 minutes after the test completed. If the

duration is more than 5 minutes, it can affect to the data. Also strong acid and alkaline range can

have negative affect to the data. Some chloride(Cl-) in the sample, the measured concentration

can be lower than the actual concentration. The concentration is lower than 100㎎/L, it does

not work as an interference. Also, ferric ion and nitrite ion may affect negatively to the result.


Collect samples using a glass container and analyze it promptly for good result. If

prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2

(about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to

room temperature and adjust pH to neutralization with sodium hydroxide solution. In case of

that sample contains suspended substances, take the sample while stirring to make the

floating materials are even in it.

※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L

Accuracy and Precision

When 1㎎/L of standard solution is investigated, check it the difference from the



Standard Solution

Dissolve 0.7218g of KNO3(Potassium Nitrate) which has been dried for more than 24 hrs at

105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution. Add 2mL of chloroform and keep it in cool and dark place. Then it is stable for 6 months.

Detection Limit

Errors are more frequent if the concentration of a sample is lower than 0.2㎎/L, so




- 115 -

Nitrogen, Nitrate


HS-NO3(N)-L Set 100/set 05020

Nitrogen, Nitrate

Contents


NO3(N)-L Powder Pillow(P) - 100Ea/set 05020-10



- 116 -

Nitrogen, Nitrate

Method 2210Brucine Method

NO3(N)(1 ~ 15mg/L)Scope and Application : water and wastewater

NO3(N)-H (1 ~ 15mg/L)

1) Add 0.5ml of distilled water to NO3(N)-H Kit to prepare reagent blank test.

2) Add 0.5ml of sample to NO3(N)-H Kit to be tested using a precise pipet.

3) Add 0.5ml of S to the each Kit, which is distilled water and sample add.

4) Shake up and down for 10 times to mix them. (Be careful with the hot kits)

Nitrogen, Nitrate



- 117 -

Nitrogen, Nitrate

5) Put the kits to reactor(HS-R 200) and heat it for 20 minutes at 100℃. Place the plastic cover on the reactor. (Make the reactor's temperature to 100℃ in advance)

6) After heating reaction, take the kits from the reactor and cool it to room temperature for 2 minutes with cold water such as tap water. Perform measurement with 3 minutes, I it is very important for the best results.

7) Perform the test . Please refer to Chapter 4. HUMAS Program for test procedure.

Nitrogen, Nitrate



- 118 -

Nitrogen, Nitrate

Nitrogen, Nitrate


than the actual concentration. The concentration is lower than 10,000㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride. If the kits under testing with NO3(N)-H Kit is turned to color from colorlessness before adding S, it means that has interference. Please contact manufacture.


prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N). In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L



Standard SolutionDissolve 0.7218g of KNO3(Potassium Nitrate) which has been dried for more than 24 hrs at

105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.Add 2mL of chloroform and keep it in cool and dark place. Then it is stable for 6 months.




- 119 -


HS-NO3(N)-H Set 25/set 05010

Nitrogen, Nitrate

Contents


NO3(N)-H Kit 1~2 25Ea/set 05010-01

NO3(N)-H Solution (S) - 1bottle/set 05010-20

Nitrogen, Nitrate



- 120 -

Nitrogen, Nitrate

Method 2220Chromatropic Method

NO3(N)-CA(0.5 ~ 30mg/L)Scope and Application : water and wastewater

NO3(N)-CA (0.5 ~ 30mg/L)

1) Add 0.5ml of distilled water to NO3(N)-CA Kit to prepare reagent blank test. (Don't add any reagent to this vial)

2) Add 0.5ml of sample to NO3(N)-CA Kit to be tested using a precise pipet.

3) After cap tightly, shake them which sample and distilled water add up and down for 10 times. Add one of Powder Pillow-1 to the kit, only added sample .

4) Cap tightly and shake the kit slowly for 10 times.

5) Leave them for reaction for 10 minutes.

6) After 10 minutes, start get measurement. Please refer to Chapter 4. HUMAS Program for test procedure.

Nitrogen, Nitrate



- 121 -

Nitrogen, Nitrate


than the actual concentration. The concentration is lower than 500㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride. The substance in the below table lie below the limit at which the determination is interfered with.


Barium 1 Copper All range

NO2 12


prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N). In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 30㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L




105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.

Nitrogen, Nitrate



- 122 -


HS-NO3(N)-CA Set 25/set 05030

Nitrogen, Nitrate

Contents


NO3(N)-CA Kit 1~2 25Ea/set 05030-01

NO3(N)-CA Powder Pillow(P) 1~2 25Ea/set 05030-10

Nitrogen, Nitrate



- 123 -

Nitrogen, Nitrite

Method 2400NED Method

NO2(N)-L(0.1 ~ 1mg/L)Scope and Application : water and wastewater

NO2(N)-L (0.1 ~ 1mg/L)



3) Add 3ml of S to each sample cell. Cap tightly and shake slowly the sample cells up and down for 10 times.

4) Add one of Powder Pillow (P) to each sample cell.

Nitrogen, Nitrite



- 124 -

Nitrogen, Nitrite

Nitrogen, Nitrite

5) Cap tightly and shake each kit vigorously for 15 seconds.

6) Leave them for reaction for 10 minutes.

7) After 10 minutes, start get measurement. Please refer to Chapter 4. Operation Guideline for test procedure.



- 125 -

Nitrogen, Nitrite

Nitrogen, Nitrite

InterferencesStrong oxidant and reducing substance in the sample will interfere. If samples contain

greater than 600mg/L Alkalinity, it can make lower reading than actual data.


result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L Accuracy and Precision

When 0.5㎎/L of standard solution is investigated, check it the difference from the actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean.

Standard Solution Dissolve 1.232g of NaNO2(Sodium Nitrite) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 250㎎/ℓ of standard solution. Add 2mL of chloroform and keep it in cool and dark place.




- 126 -


HS-NO2(N)-L Set 100/set 04012

Nitrogen, Nitrite

Contents


NO2(N)-L Solution (S) - 1bottle/set 04012-20

NO2(N)-L Powder Pillow(P) 1~2 100Ea/set 04012-10

Nitrogen, Nitrite



- 127 -

Nitrogen, Nitrite

Nitrogen, Nitrite

Method 2410FSS Method

NO2(N)-H(5 ~ 150mg/L)Scope and Application : water and wastewater

NO2(N)-H (5 ~ 150mg/L)


2) Add 10ml of sample to be tested another sample cell ( round glass vial) using precise pipet.

3) Add 1ml of S to each sample cell. Cap tightly and shake slowly the sample cells up and down for 10 times.

4) Add one of Powder Pillow (P) to each sample cell.

5) Close it with the cap and shake them up and down slowly for 10 times to mix them.

6) After 10 minutes, wipe the cells and place it into cell holder and read the result. Please refer to chapter 4. Operation Guideline of Instrument Manual for test procedure.



- 128 -

Nitrogen, Nitrite

Nitrogen, Nitrite

InterferencesStrong oxidant and reducing substance in the sample will interfere. If samples contain

greater than 600mg/L Alkalinity, it can make lower reading than actual data.


result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid add for preservation, is tested in 30㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L Accuracy and Precision


Standard Solution Dissolve 1.232g of NaNO2(Sodium Nitrite) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 250㎎/ℓ of standard solution. Add 2mL of chloroform and keep it in cool and dark place.




- 129 -


HS-NO2(N)-H Set 100/set 04014

Nitrogen, Nitrite

Contents


NO2(N)-H Solution (S) - 1bottle/set 04014-20

NO2(N)-H Powder Pillow(P) 1~2 100Ea/set 04014-10

Nitrogen, Nitrite



- 130 -

Nitrogen, Total

Method 2000Cadmium Reduction Method

TN-U(0.2 ~ 5mg/L)Scope and Application : water and wastewater

TN-U (0.2 ~ 5mg/L)

1) To prepare the reagent blank test, add 2ml of distilled water to TN-U Kit.

2) Take 2㎖ of a sample to be tested using a precise pipette and add to different TN-U Kit.

3) Add Powder Pillow-1(P1) of one to each TN-U Kit(vial).


Nitrogen, Total



- 131 -

Nitrogen, Total

Nitrogen, Total

5) Put the kits to reactor(HS-R 200) and heat it for 30 minutes at 120℃. Place the plastic cover on the reactor. After heating reaction, cool the kits to room temperature with cold water such as tap water. (The heating time is very important for the best result)

6) Add Power Pillow-2(P2) of one to each kit and shake vigorously the vials for one minute.



- 132 -

Nitrogen, Total

Nitrogen, Total

7) Add Power Pillow-3(P3) of one to each kit and shake the vials up and down for 10 times to mix them.

8) After leave them for 5 minutes and measure it. See Chapter 4. HUMAS Proram of the Instrument Manual and measure the samples.



- 133 -

Nitrogen, Total

Nitrogen, Total

InterferencesThe colored solution and suspended substances in the sample will interfere. Also,

strong oxidizing and reducing materials will interfere. Chloride(Cl-) concentration above 100㎎/L will cause low results. If ferric ion and nitrite ion in the samples, those work as interference. It should be measured within 3 minutes after testing for the best results. Collection and Preservation

Collect samples using a glass container and analyze it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and neutralize (pH 5 approximately) with 5N Sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of Sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,002㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration 10㎎/L × 1.006 = 10.06㎎/L





Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.2㎎/L.



- 134 -

Nitrogen, Total


HS-TN-U Set 25/set 02011

Nitrogen, Total

Contents


TN-U Kit 1~2 25Ea/set 02011-01

TN-U Powder Pillow-1(P1) 1~2 25Ea/set 02011-10





- 135 -

Nitrogen, Total

Method 2020Chromotropic acid Method

TN-L(CA)(1 ~ 50mg/L)Scope and Application : water and wastewater

TN-L(CA) (1 ~ 50mg/L)

1) Add 2ml of distilled water to TN-L(CA) Kit(Black Cap) to prepare reagent blank test.

2) Add 2ml of sample to another TN-L(CA) Kit(Black Cap) to be tested using a precise pipet.

3) Add Powder Pillow-1(P1) of one to the each Kit(Black Cap), which is distilled water and sample add.

4) Close it with the cap and shake it up and down slowly for 10 times to mix them.

Nitrogen, Total



- 136 -

Nitrogen, Total

5) Put the kits to reactor(HS-R 200) and heat it for 30 minutes at 120℃. Place the plastic cover on the reactor. (Make the reactor's temperature to 150℃ in advance) ※When external temperature is too low, white sediments which may affect to data can be made.

6) After heating reaction, take the kits from the reactor and cool it to room temperature with cold water such as tap water. Then, add Powder Pillow-2(P2) and shake it vigorously for 30 seconds to dissolve it completely. Then make it react for 3 minutes.

7) Add Powder Pillow-3(P3) and shake them up and down for 10 times to mix them. Then make the kits react for 2 minutes.

8) Take 2ml of the sample from the TN-L(CA) (Blcak cap) and add respectively to two TN-L(CA) Kits (White cap). Shake it up and down for 10 times to mix them. (Be careful with the hot kits)

Nitrogen, Total



- 137 -

Nitrogen, Total

Nitrogen, Total

9) After reaction for 10 minutes, perform the test . Please refer to Chapter 4. Operation Guideline for test procedure of the Instrument Manual.



- 138 -

Nitrogen, Total

Nitrogen, Total


than the actual concentration. The concentration is lower than 800㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride. Sample containing more than 0.5mg/L ferrous, it works as a interference and can be caused higher result than actual concentration. Not exceeding pH 12 of the sample, it does not work as an interference. Sample containing more than 300mg/L COD, it may cause to higher result.


prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and neutralize pH to 7 with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L








- 139 -


HS-TN-L(CA) Set 25Ea/set 02022

Nitrogen, Total

Contents


TN-L(CA) Kit 1~2 25Ea/set 02022-01

TN-L(CA) Powder Pillow-1 1~2 25Ea/set 02022-10



Nitrogen, Total



- 140 -

Nitrogen, Total

Method 2021Chromotropic acid Method

TN-H(CA)(10 ~ 100mg/L)Scope and Application : water and wastewater

TN-H(CA) (10 ~ 100mg/L)

1) Add 0.5ml of distilled water to TN-H(CA) Kit(Black cap) to prepare reagent blank test.

2) Add 0.5ml of sample to another TN-H(CA) Kit(Black cap) to be tested using a precise pipet.

3) Add Powder Pillow-1(P1) of one to the each Kit(Black cap), which is distilled water and sample add.

4) Close it with the cap and shake it up and down slowly for 10 times to mix them.

Nitrogen, Total



- 141 -

Nitrogen, Total

Nitrogen, Total

5) Put the kits to reactor(HS-R 200) and heat it for 30 minutes at 120℃. Place the plastic cover on the reactor. (Make the reactor's temperature to 150℃ in advance) ※ When external temperature is too low, white sediments which may affect to data can be made

6) After heating reaction, take the kits from the reactor and cool it to room temperature with cold water such as tap water. Then, add Powder Pillow-2(P2) and shake it vigorously for 30 seconds to dissolve it completely. Then make it react for 3 minutes.

7) Add Powder Pillow-3(P3) and shake them up and down for 10 times to mix them. Then make the kits react for 2 minutes.

8) Take 1ml of the sample from the TN-H(CA) (Black cap) and add respectively to two TN-H(CA) Kits (White cap). Shake it up and down for 10 times to mix them. (Be careful with the hot kits)



- 142 -

Nitrogen, Total

Nitrogen, Total

9) After reaction for 10 minutes, perform the test . Please refer to Chapter 4. Operation Guideline for test procedure of the Instrument Manual.



- 143 -

Nitrogen, Total

Nitrogen, Total


than the actual concentration. The concentration is lower than 800㎎/L, it does not work as an interference. If it is higher than that, the sample need to be diluted to proper concentration of chloride. Sample containing more than 0.5mg/L ferrous, it works as a interference and can be caused higher result than actual concentration. Not exceeding pH 12 of the sample, it does not work as an interference. Sample containing more than 300mg/L COD, it may cause to higher result.


prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and neutralize pH to 7 with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L




105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution. Add 2ml of chloroform and keep it in cool and dark place. Then it is stable for 6 months.

Detection Limit Errors are more frequent if the concentration of a sample is lower than 10㎎/L, so




- 144 -


HS-TN-H(CA) Set 25Ea/set 02024

Nitrogen, Total

Contents


TN-H(CA) Kit 1~2 25Ea/set 02024-01

TN-H(CA) Powder Pillow-1 1~2 25Ea/set 02024-10



Nitrogen, Total



- 145 -

Orthophosphate

Orthophosphatel

Method 3020Ascorbic acid Method

PO4(P)-L(0.01 ~ 3mg/L)Scope and Application : water and wastewater

PO4(P)-L (0.01 ~ 3mg/L)

1) Add 5ml of distilled water to sample cell(round glass cell) to prepare reagent blank test.

2) Add 5ml of sample to another sample cell(round glass cell) to be tested using a precise pipet.

3) Add 6ml of S to each sample cell.

4) Add one of Powder Pillow(P) to each sample cell, which is distilled water and sample add.

5) Cap tightly and shake it up and down slowly for 10 times to mix them. This test needs reaction time for 10 minutes.

6) After 10 minute reaction, wipe the cells and place it into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.



- 146 -

Orthophosphate

Orthophosphate

Interferences Ferric, copper, and silicate ion in the sample, that are not work as an interferences. However,

high concentration of ferric ion may make deposits and become interferences. When strong oxidizing or reducing agent is contained, it will may interfere. If the concentration of Arsenic is higher than the one of TP, it can affect on the test as an interference. The concentration level of that in seawater has no effective. When the concentration of Cl- is higher than 7,000 ㎎/ℓ, test value shall be lower than the actual one. It should be diluted to proper concentration of chloride for the best results.


result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N approximately). In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L



Standard Solution Dissolve 0.439g of KH2PO4(Potassium Dihydrogenphosphate) which has been dried for more than 24 hrs at 105 ℃ in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.




- 147 -


HS-PO4(P)-L Set 100/set 07012

Orthophosphate

Contents


PO4(P)-L Solution(S) - 1 bottle/set 07012-20

PO4(P)-L Powder Pillow(P) 1~2 100Ea/set 07012-10

Orthophosphate



- 148 -

Orthophosphate

Method 3030Molybdo Vanadate Method

PO4(P)-H(1 ~ 15mg/L)Scope and Application : water and wastewater

PO4(P)-H (1 ~ 15mg/L)

1) Add 5ml of distilled water to (round glass cell) to prepare reagent blank test.

2) Add 5ml of sample to another sample cell (round glass cell) to be tested using a precise pipet.

3) Add 6ml of S to each sample cell.

4) Cap the cells tightly, and shake it up and down for 10 times to mix the cells.

5) After 10 minute reaction, wipe the cells and place it into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.

Orthophosphate



- 149 -

Orthophosphate

Orthophosphate

InterferencesFerric, copper, and silicate ion in the sample, that are not work as an interferences. However,

upper concentration of 100 mg/L ferric ion may make deposits and become interferences. When strong oxidizing or reducing agent is contained, it will may interfere. When the concentration of Cl- is higher than 20,000 ㎎/ℓ, test value shall be lower than the actual one. It should be diluted to proper concentration of chloride for the best results. Sample Collection and Preservation

Collect samples using a glass and plastic container and analyze it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L







- 150 -


HS-PO4(P)-H Set 100/set 07014

Orthophosphate

Contents


PO4(P)-H Solution (S) - 1 bottle/set 07014-20

Orthophosphate



- 151 -

Phenol

Method 4500

Direct photometric Method

Phenol(0.1 ~ 10mg/L)


Phenol (0.1 ~ 10mg/L)


cell) to prepare reagent blank test.


cell) to be tested using a precise pipet.

3) Add 0.2ml of S-1 to each cell. Cap tightly and shake it

up and down slowly for 10 times to mix it.

4) Add 0.2ml of S-2 to each kit, Cap tightly and shake it


5) Add 0.2ml of S-3 to each cell. Cap tightly and shake it


6) Take reaction time for 15 minutes, and wipe the cells

and place it into cell holder and read the result. Please

refer to chapter 4. HUMAS Program of Instrument Manual for

test procedure.

Phenol



- 152 -

Phenol

Phenol

InterferencesStrong oxidant and reducing substance in the sample will interfere. For the best result, adjust

the pH of sample to 3~11.5. If sample to be tested is colorful, other than transparent, it can affect to

the actual data.

Sample Collection and PreservationCollect samples using glass and plastic container and analyze it promptly for good

result. A kind of phenol is decomposed easily by reducing and oxidizing reagent and bacteria,

so, it should be analyzed within 4 hours after collecting. If prompt analysis is not possible,

add 10 % phosphorous acid to pH of less than 4. And, add 5g of Copper sulfate per 1L of

the collected sample. It can be stored for 24 hours at dark place.




Standard Solution Dissolve 1g of Phenol in distilled water, and make it 1L precisely. It becomes 1000㎎/ℓ of

standard solution.





- 153 -


HS-Phenol Set 100Ea/set 09012

Phenol

Phenol

Contents



Phenol Solution (S-1) - 1bottle/set 09012-20





- 154 -

Phosphorous, Total

Method 3000Ascorbic acid Method

TP-L(0.01 ~ 3mg/L)Scope and Application : water and wastewater

TP-L (0.01 ~ 3mg/L)

1) Add 5ml of distilled water to TP-L Kit to prepare reagent blank test.

2) Add 5ml of sample to TP-L Kit to be tested using a precise pipet.

3) Add one of Powder Pillow-1(P1) to each kit, which is distilled water and sample add.

4) Cap the kits tightly and shake it up and down slowly for 10 times to mix it.

Phosphorous, Total



- 155 -

Phosphorous, Total

Phosphorous, Total


6) After heating it for 30 minutes at 120 ℃, cool it off by cold water until it reaches to room temperature. Then, add 2ml of S-1 to each kit and shake the vials up and down slowly for 10 times to mix it.

7) Add 2mL of S-2 and close it with cover. Shake it up and down slowly for 10 times.



- 156 -

Phosphorous, Total

Phosphorous, Total

8) Add one of Powder Pillow-2(P2) and close it with cover. Shake it up and down slowly for 10 times.

9) After 10 minute reaction, wipe and place the vials into cell holder to read the result. Please refer to Chapter 4. HUMAS Program of Instrument Manual for test procedure.



- 157 -

Phosphorous, Total

Phosphorous, Total

Interferences Ferric, copper, and silicate ion in the sample, that are not work as an interferences. However,

high concentration of ferric ion may make deposits and become interferences. When strong oxidizing or reducing agent is contained, it will may interfere. If the concentration of Arsenic is higher than the one of TP, it can affect on the test as an interference. The concentration level of that in seawater has no effective. When the concentration of Cl- is higher than 7,000 ㎎/ℓ, test value shall be lower than the actual one. It should be diluted to proper concentration of chloride for the best results.


result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution(5N approximately). In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L







- 158 -


HS-TP-L Set 25/set 03012

Phosphorous, Total

Contents


TP-L Kit 1~2 25Ea/set 03012-01

TP-L Powder Pillow-1(P1) 1~2 25Ea/set 03012-10

TP-L Powder Pillow-2(P2) 1~2 25Ea/set 03012-11

TP-L Solution(S-1) - 1bottle/set 03012-20

TP-L Solution(S-2) - 1bottle/set 03012-21

Phosphorous, Total



- 159 -

Phosphorous, Total

Method 3010Molybdo Vanadate Method

TP-H(1 ~ 15mg/L)Scope and Application : water and wastewater

TP-H (1 ~ 15mg/L)

1) Add 5ml of distilled water to TP-H Kit to prepare reagent blank test.

2) Add 5ml of sample to TP-H Kit to be tested using a precise pipet.

3) Add one of Powder Pillow(P) to each kit, which is distilled water and sample add.

4) Cap the kits tightly and shake it up and down slowly for 10 times to mix the vials.

Phosphorous, Total



- 160 -

Phosphorous, Total

Phosphorous, Total


6) After the time is over, remove the vials from the reactor and cool it to reach room temperature by cold water such tap water. Add 2ml of S-1 to each kit and shake it up and down slowly for 10 times to mix the vials.

7) Add 2ml of S-2 and cap the vials tightly. Then, to mix the solution, shake the vials up and down slowly for 10 times.



- 161 -

Phosphorous, Total

Phosphorous, Total

8) After 10 minute reaction, wipe and place the vials into cell holder to read results. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.



- 162 -

Phosphorous, Total

Phosphorous, Total

InterferencesFerric, copper, and silicate ion in the sample, that are not work as an interferences. However,

upper concentration of 100 mg/L ferric ion may make deposits and become interferences. When strong oxidizing or reducing agent is contained, it will may interfere. When the concentration of Cl- is higher than 20,000 ㎎/ℓ, test value shall be lower than the actual one. It should be diluted to proper concentration of chloride for the best results. Sample Collection and Preservation

Collect samples using a glass and plastic container and analyze it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution. In case of that sample contains suspended substances, take the sample while stirring to make the floating materials are even in it. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L







- 163 -


HS-TP-H Set 25Ea/set 03014

Phosphorous, Total

Contents


TP-H Kit 1~2 25Ea/set 03014-01

TP-H Powder Pillow(P) 1~2 25Ea/set 03014-10

TP-H Solution(S-1) - 1bottle/set 03014-20

TP-H Solution(S-2) - 1bottle/set 03014-21

Phosphorous, Total



- 164 -

Silica

Method 5500

Molybdosilicate Method

Silica(0.5 ~ 20mg/L)


Silica-L (0.5 ~ 20mg/L)





3) Add Powder Pillow-1(P1) and shake them up and down

slowly for 10 times to mix them.


slowly for 30 seconds to mix them. And, wait for 10

minutes.


slowly for 30 seconds to mix them.

6) After 3minute reaction, wipe the cells and place it into

cell holder and read the result. Please refer to chapter

4. HUMAS Program of Instrument Manual for test

procedure.

Silica



- 165 -

Silica

InterferencesStrong oxidant and reducing substance in the sample will interfere. Large amount of iron,

color turbidity, sulfide and phosphate will interfere.

Sample Collection and PreservationTo collect samples using an plastic container and avoid using glassware as much as

possible and analyze it promptly for good result. If prompt analysis is not possible, it can be

stored for 7days at room temperature. Before, analyzing, warm sample to room temperature.




Standard Solution Use commercial standard solution(1000mg/L) to check accuracy. It should be dilute to

proper concentration that is within the range.

Detection Limit Errors are more frequent if the concentration of a sample is lower than 0.5㎎/L and

higher than 20mg/L, so test should be performed within appropriate concentration range.

Silica



- 166 -

Silica


HS-Si-L Set 100Ea/set 44012

Contents


Si-L Powder Pillow -1(P1) - 100Ea/set 44012-10



Silica



- 167 -

Silica

Method 5510

Molybdosilicate Method

Silica(20 ~ 100mg/L)


Silica-H (20 ~ 100mg/L)






slowly for 10 times to mix them.


slowly for 30 seconds to mix them. And, wait for 10

minutes.


slowly for 30 seconds to mix them.

6) After 3minute reaction, wipe the cells and place it into

cell holder and read the result. Please refer to chapter

4. HUMAS Program of Instrument Manual for test

procedure.

Silica



- 168 -

Silica

InterferencesStrong oxidant and reducing substance in the sample will interfere. Large amount of iron,

color turbidity, sulfide and phosphate will interfere.

Sample Collection and PreservationTo collect samples using an plastic container and avoid using glassware as much as

possible and analyze it promptly for good result. If prompt analysis is not possible, it can be

stored for 7days at room temperature. Before, analyzing, warm sample to room temperature.




Standard Solution Use commercial standard solution(1000mg/L) to check accuracy. It should be dilute to

proper concentration that is within the range.

Detection Limit Errors are more frequent if the concentration of a sample is lower than 20㎎/L and

higher than 100mg/L, so test should be performed within appropriate concentration range.

Silica



- 169 -


HS-Si-H Set 100Ea/set 44014

Silica

Contents


Si-H Powder Pillow-1(P1) - 100Ea/set 44014-10



Silica



- 170 -

Sulfate

Method 4600Gravimetric MethodSO4-L(5 ~ 50mg/L)


SO4-L (5 ~ 50mg/L)

1) Add 10ml of distilled water to sample cell (round glass cell) to prepare reagent blank test.


3) Add 1ml of S to each sample cell and shake them up and down slowly for 10 times to mix them.

4) Add one of Powder Pillow(P) to each sample cell and shake them up and down slowly for 10 times to mix them.

5) After 2 minutes reaction, wipe and place the cells into cell holder to read the result. Please refer to Chapter 4. HUMAS Program of Instrument Manual for test procedure.

Sulfate



- 171 -

Sulfate

Sulfate

InterferencesIf there is some silica in the sample, the concentration is lower than 5,00 ㎎/L, it

does not work as an interference. If sample to be tested is colorful, other than transparent, it can affect to the actual data. Floating substance in the sample works as an interference to the test.


promptly for good result. If prompt analysis is not possible, it can be stored for 7days at 4℃. Before, analyzing, warm sample to room temperature.


actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean. Standard Solution Dissolve 1.813g of K2SO4(Potassium Sulfate) in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution. Also, commercial standard solution is can be used. Detection Limit Errors are more frequent if the concentration of a sample is lower than 5㎎/L, so test should be performed within appropriate concentration range.



- 172 -


HS-SO4-L Set 100/set 08012

Sulfate

Contents


SO4-L Solution(S) - 1bottle/set 08012-20

SO4-L Powder Pillow(P) 1~2 100Ea/set 08012-10

Sulfate



- 173 -

Sulfide

Method 4601Gravimetric Method

SO4-H(10 ~ 70mg/L)Scope and Application : water and wastewater

SO4-H (10 ~ 70mg/L)



3) Add 1ml of S to each sample cell and shake them up and down slowly for 10 times to mix them.

4) Add one of Powder Pillow(P) to each sample cell and shake them up and down slowly for 10 times to mix them.

5) After 2 minutes reaction, wipe and place the cells into cell holder to read the result. Please refer to Chapter 4. HUMAS Program of Instrument Manual for test procedure.

Sulfate



- 174 -

Sulfate

Sulfate

InterferencesIf there is some silica in the sample, the concentration is lower than 5,00 ㎎/L, it

does not work as an interference. If sample to be tested is colorful, other than transparent, it can affect to the actual data. Floating substance in the sample works as an interference to the test.


promptly for good result. If prompt analysis is not possible, it can be stored for 7days at 4℃. Before, analyzing, warm sample to room temperature.


actual concentration of the standard solution is within 5%. Analyze the same sample more than 7 times and get a standard deviation and arithmetic mean. Standard Solution Dissolve 1.813g of K2SO4(Potassium Sulfate) in distilled water, and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution. Also, commercial standard solution is can be used. Detection Limit Errors are more frequent if the concentration of a sample is lower than 5㎎/L, so test should be performed within appropriate concentration range.



- 175 -


HS-SO4-H Set 100/set 08014

Sulfate

Sulfate

Contents


SO4-H Solution (S) - 1bottle/set 08014-20

SO4-H Powder Pillow(P) 1~2 100Ea/set 08014-10



- 176 -

Sulfide

Method 5400Methylene blue Method

S-2(0.5 ~ 5mg/L)Scope and Application : water and wastewater

S-2 (0.5 ~ 5mg/L)



3) Add 5ml of S-1 to each cell, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.

4) Add 0.5ml of S-2 to each cell, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.

5) Add 1ml of S-3 to each cell, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.

Sulfide



- 177 -

Sulfide

Sulfide

6) Add 0.5ml of S-4 to each cell, cap and shake the cells up and down slowly for 10 times.

7) Wait 10 minutes for maximum color development. At end of 2 minute waiting, insert the vial into cell holder to read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.



- 178 -

Sulfide

Sulfide


Floating substance in the sample works as an interference to the test. A reducing, sulfite, thiosulfate and hydrosulfite in the sample will interfere. High buffered sample is need to dilute proper concentration for the test.





Standard Solution Dissolve 0.75g of Na29H2O(Sodium Sulfide 9-hydrate, crystal) in distilled water, and make it 1L precisely. It becomes 100㎎/ℓ of standard solution.




- 179 -


HS-S2- Set 100/set 37010

Sulfide

Contents


S-2 Solution(S-1) - 1bottle/set 37010-20




Sulfide



- 180 -

Turbidity

Method 6700Absorption MethodTurb.(0 ~ 400FTU)

Scope and Application : water and wastewate

Turbidity(0 ~ 400FTU)

1) Add 5ml of distilled water to sample cell to prepare reagent blank test.

2) Add 5ml of sample to another sample cell to be tested using a precise pipet.

3) Wipe place the cells into the cell holder to read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.

Turbidity



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Zinc

Method 4200Zincon Method

Zn(0.1 ~ 5mg/L)Scope and Application : water and wastewater

Zn (0.1 ~ 5mg/L)



3) Add 2ml of S-1 to each sample cell.

4) Add one of Powder Pillow(P) to each sample cell.

5) Cap the cells tightly and shake the cells up and down gently for 10 times.

Zinc



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Zinc

Zinc

6) Add 0.9ml of S-2 to each cell, which is distilled water and sample add. Cap tightly and shake it up and down slowly for 10 times to mix it.

7) Add 0.5ml of S-3 to each cell, cap tightly and shake it up and down slowly for 10 times to mix it.

8) After one minute, Wipe the cells and place it into cell holder and read the result. Please refer to chapter 4. HUMAS Program of Instrument Manual for test procedure.



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Zinc

Zinc

Interferences The following substances have been tested for interference and showed not work as interference up to indicated levels.


Aluminum 6

Cadmium 0.5

Iron 7

Manganese 5

Nickel 5

Copper 5


Collect samples using an acid-cleaned glass and plastic container and analyze it promptly for good result. If prompt analysis is not possible, add 2㎖ of concentrated sulfuric acid to pH of less than 2 (about 2㎖ per liter). It can be stored for 28days at 4℃. Before, analyzing, warm sample to room temperature and adjust pH to neutralization with sodium hydroxide solution. Floating substance in the sample may will interfere. ※ Compensation as adding the reagent Ex.) When sample which has 2ml of sulfuric acid and 4ml of sodium hydroxide solution add for preservation, is tested in 10㎎/L, apply compensation value by adding reagent and calculate the actual concentration by the below expressions. ○ Total volume = 1,000㎖ + 2㎖ + 4㎖ = 1,006㎖○ Calculating compensation value = 1,006/1,000 =1.006○ Actual Concentration = 10㎎/L × 1.006 = 10.06㎎/L



Standard Solution Dissolve 1g of Zinc in 10 mL of HNO3(1+1). To eliminate nitric oxidant from the solution, heat the solution after adding some distilled water and make it 1L precisely. It becomes 1,000㎎/ℓ of standard solution.




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HS-Zn Set 100/set 17010

Zinc

Contents


Zn Powder Pillow(P1) 1~2 100Ea/set 17010-10

Zn Solution(S-1) - 1bottle/set 17010-20



Zinc

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