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Title Dr First Name Seema Last Name Makhija Photograph
Designation Associate Professor
Address Ciliate Biology Laboratory Acharya Narendra Dev College Govindpuri, Kalkaji New Delhi 110019
Phone No Office 26294541
Residence
Mobile 9718467014
Email seemamakhija@andc.du.ac.in
Web-Page
Educational Qualifications
Degree Institution Year
B.Sc. (H) Zoology University of Delhi 1992
M.Sc. Zoology University of Delhi 1994
Ph.D. University of Delhi 2001
Career Profile
Faculty Details proforma for DU Web-site
(PLEASE FILL THIS IN AND Email it to websiteDU@du.ac.in and cc: director@ducc.du.ac.in
www.du.ac.in Page 2
Administrative Assignments
Areas of Interest / Specialization
Biodiversity of Ciliates, Fresh water and Soil Bioinformatics Biotechnology
Subjects Taught
B.Sc. (H) Zoology Evolution Cell Biology Molecular Biology
Research Guidance
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Publications Profile
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➢ Seema Arora, Renu Gupta, Swati Machwe and Gulshan Rai Sapra. (1999) Influence of cadmium on
development of surface ciliary structures in the ciliate Stylonychia mytilus (Ciliophora, Oxytrichidae).
Europ. J. Protistol. 35, 281-289.
➢ Seema Arora, Renu Gupta, Komal Kamra and Gulshan Rai Sapra. (1999) Characterization of
Paraurostyla coronata sp. n. including a comparative account of other members of the genus. Acta
Protozool. 38, 133-144.
➢ Renu Gupta, Komal Kamra, Seema Arora and Gulshan Rai Sapra. Stylonychia ammermanni sp. n. a new
oxytrichid (Ciliophora, Hypotrichida) ciliate from the river Yamuna, Delhi, India. Acta Protozool. 40, 75-
82 (2001).
➢ Swati Machwe, Seema Arora, Renu Gupta and G.R. Sapra. (2001) Cisplatin induces modifications in the
development of cell surface patterns of ciliates. Cell Biol. Intl. 25 (11), 1131-1138.
➢ Arora, S., Gupta, R., Sapra, G. R. (2002). Developmental toxicity of lithium in the ciliate, Stylonychia.
The Jap. Journ. Protozool. Vol. 35 (suppl.), 42.
➢ Gupta, R., Arora, S., Kamra, K., Sapra, G. R. (2002). Biodiversity of genus Oxytricha in Delhi region of
the Indian Subcontinent. The Jap. Journ. of Protozoology, Vol. 35 (suppl.), 30.
➢ Renu Gupta, Komal Kamra, Seema Arora and Gulshan Rai Sapra (2003). Pleurotricha curdsi (Shi,
Warren and Song 2002) nov. comb. (Ciliophora: Hypotrichida): morphology and ontogenesis of an Indian
population; redefinition of the genus. Europ. J. Protistol. 39, 275-285.
➢ Makhija S, Gupta R and Toteja R (2015) Lithium-induced developmental anomalies in the spirotrich
ciliate, Stylonychia mytilus (Ciliophora, Hypotrichida). European Journal of Protistology, 51: 290-298.
➢ Makhija S, Gupta R, Toteja R, Abraham JS and Sripoorna S (2015) Cadmium induced ultrastructral
changes in the ciliate, Stylonychia mytilus (Ciliophora, Hypotrichida). Journal of Cell and Tissue
Research, 15 (3): 5151-5157.
➢ Sripoorna S, Abraham JS, Gupta R, Makhija S and Toteja R (2015) Diverse freshwater spirotrich ciliate
fauna from Okhla Bird Sanctuary, Delhi, India. Global Journal for Research Analysis, 4 (9): 37-41.
➢ Ravi Toteja, Seema Makhija, S. Sripoorna, Jeeva Susan Abraham and Renu Gupta (2015) Influence of
Copper and Cadmium Toxicity on the Activity of an Antioxidant Enzyme, Superoxide Dismutase in
Freshwater Ciliates. Indian Journal of Experimental Biology (Accepted for Publication).
➢ Ashish Choudhary, Jeeva Susan Abraham, S. Sripoorna, Manu Sankar, Vikas Ahlawat, Renu Gupta,
Seema Makhija, Ravi Toteja (2016) Evaluation of the cytotoxic effect of pesticide malathion on
freshwater ciliate, Tetmemena sp. DU Journal (Accepted for Publication).
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PAPERS SUBMITTED
Jeeva Susan Abraham, S. Sripoorna, Ashish Choudhary, Ravi Toteja, Renu Gupta, Seema Makhija and
Alan Warren (2016) Assessment of Heavy Metal Toxicity in Four Species of Freshwater Ciliates
(Spirotrichea; Ciliophora) from Delhi, India. International Journal of Environmental Technology and
Management.
Ilmas Naqvi, Renu Gupta, Seema Makhija, Ravi Toteja, Jeeva S Abraham, S. Sripoorna and Hameed
A.El-Serehy (2016) Morphology and morphogenesis of a new oxytrichid ciliate, Notohymena quadrii n.
sp. (Ciliophora, Oxytrichidae) from Delhi, India. Saudi Journal of Biological Sciences.
HOLOTYPE SUBMISSION OF NEW CILATE SPECIES TO ZOOLOGICAL SURVEY OF INDIA
Abraham J.S. Somasundaram S., Toteja R., Makhija S. and Gupta R. (2016). Notohymena
amicronuleata sp.nov. Acc. No.: Pt.3220.
GENEBANK SUBMISSIONS
➢ Abraham, J. S., Somasundaram, S., Gupta, R., Toteja, R. and Makhija, S. (2015) Notohymena sp. SL1
18S rRNA gene, partial sequence. Acc no. KP336402.1.
➢ Abraham, J. S., Somasundaram, S., Gupta, R., Toteja, R. and Makhija, S. (2015) Pseudourostyla
cristata strain SL2 18S ribosomal RNA gene, partial sequence. Acc. No. KT731104.
➢ Abraham, J. S., Somasundaram, S., Gupta, R., Toteja, R. and Makhija, S. (2015) Gastrostyla sp. Y2 18S
ribosomal RNA gene, partial sequence. Acc. No. KT780432.
➢ Somasundaram, S., Abraham, J. S., Gupta, R., Makhija, S. and Toteja, R. (2015) Tetmemena sp. Y1 18S
rRNA gene, partial sequence. Acc no. KP336401.1.
➢ Somasundaram, S., Abraham, J. S., Gupta, R., Toteja, R. and Makhija, S. (2015) Tetmemena sp. Y1 18S
ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, internal
transcribed spacer 2, complete sequence; 28S ribosomal gene, partial sequence. Acc. No. KT731103.
➢ Somasundaram, S., Abraham, J. S., Toteja, R., Makhija, S. and Gupta, R. (2016) Tetmemena sp. Y1
histone H4 gene, partial cds. Acc. No. KU761846
➢ Abraham, J. S., Somasundaram, S., Makhija, S., Toteja, R. and Gupta, R. (2016) Oxytricha
granulifera 18S ribosomal RNA gene, partial sequence. Acc. No. KU715983
➢ Makhija, S., Gupta, R., Toteja, R., Abraham, J. S. and Somasundaram, S. (2016) Paraurostyla
coronata 18S ribosomal RNA gene, partial sequence. Acc. No. KU715982
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E-CHAPTER
Seema Makhija (2008) Origin of Life. (http:// nsdl.niscair.res.in/handle),National Science Digital Library
created by CSIR organization, National Institute of Science and Communication and Information
Resources (NISCAIR).
LABORATORY MANUAL PROTOCOLS
➢ Seema Makhija, Ravi Toteja (2008) To perform agarose gel electrophoresis of DNA samples. Biology
Laboratory Course Workshop Manual.
➢ Seema Makhija (2008) To perform Western Blotting. Biology Laboratory Course Workshop Manual.
➢ Seema Makhija, Ravi Toteja (2008) To perform Southern Blotting. Biology Laboratory Course
Workshop Manual.
➢ Seema Makhija (2008) Finger Printing. Biology Laboratory Course Workshop Manual.
EDUCATIONAL CONTENT IN OPEN DOMAIN
➢ Seema Makhija (2010) Educational content on Structure and functions of Proteins
http://eduframe.net/ANDC/biology/Ravi-Bio/Ravi%20proteinsODL.htm.
➢ Seema Makhija (2013) Educational content on Cytochemical demonstration of DNA by Feulgen
reaction. http://eduframe.net/CamTelANDC/OpenVideo.php
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Conference Organization/ Presentations (in the last three years)
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➢ Prateek Kumar, Kritika Shukla, Parveen Atrish, Jeeva Susan, Seema Makhija and Ravi Toteja (2013)
“Use of Bioluminescent Ciliate Sensors as an Alternative Method for Measuring Heavy Metals in Fresh
Water Samples” National Conference, Redefining Science Teaching: Future of Education held at
University of Delhi South Campus, March 7-9, 2013.
➢ Monisha Khanna, Ravi Toteja, Vikrant Atri (2013) “Screening of soil actinomycetes for antimicrobial
compounds” at National Conference on “Redefining Science teaching: Future of education” held at
University of Delhi South Campus, March 7-9, 2013.
➢ S. Sripoorna, Jeeva Susan Abraham, Seema Makhija, Renu Gupta and Ravi Toteja (2013) “Ciliate
microbial biodiversity at Sanjay Lake, Delhi” in Annual Conference of Association of
Microbiologists of India (AMI-2013) held at Maharshi Dayanand Univesity, Rohtak Haryana, November
17-20, 2013
➢ Jeeva Susan Abraham, S. Sripoorna, Ravi Toteja, Renu Gupta and Seema Makhija (2013) “Bio-
Monitoring heavy metal pollution in fresh water bodies by single celled eukaryotic microorganisms” in
Annual Conference of Association of Microbiologists of India (AMI-2013) held at Maharshi Dayanand
Univesity, Rohtak Haryana, November 17-20, 2013.
➢ Sarita Kumar, Ravi Toteja and Charu K Gupta (2014) Creation of E-museum as Open Educational
Resource Repository of Organisms for Students of Biological Sciences. International conference „OCWC
Global 2014‟ Ljubljana, Slovenia, April 23-25, 2014.
➢ Seema Makhija, Renu Gupta and Ravi Toteja (2014) “Diverse freshwater ciliate Fauna from Okhla Bird
Sanctuary, Delhi, India. 3rd
IRCN-BC Workshop held at Royal Holloway, University of London, U.K.
➢ Renu Gupta, Seema Makhija and Ravi Toteja (2014) Soil ciliate (Protozoa, Ciliophora) Diversity in and
around Delhi, India. 3rd
IRCN-BC Workshop held at Royal Holloway, University of London, U.K.
➢ Jeeva Susan Abraham, Renu Gupta, S. Sripoorna, Ravi Toteja and Seema Makhija (2014) Morphology,
Morphogenesis and Molecular Phylogeny of Notohymena sp. (Ciliophora; Oxytrichidae) isolated from
Sanjay Lake, Delhi, India, 2nd
Asian congress of Protistology and Ninth Asian Conference on Ciliate
Biology organized by Kalyani University, Kalyani, India.
➢ S. Sripoorna, Seema Makhija, Jeeva Susan Abraham, Renu Gupta and Ravi Toteja (2014) Inferring
Phylogenetic Position of an Indian Isolate Tetmemena sp. (Ciliophora, Oxytrichidae) Using
Morphological, Morphogenetic and Molecular Markers. 2nd
Asian congress of Protistology and Ninth
Asian Conference on Ciliate Biology organized by Kalyani University, Kalyani, India.
➢ Ravi Toteja, Seema Makhija, S. Sripoorna, , Jeeva Susan Abraham and Renu Gupta and (2014)
Influence of Copper and Cadmium Toxicity on the Activity of an Antioxidant Enzyme, Superoxide
Dismutase in Freshwater Ciliates. 2nd
Asian congress of Protistology and Ninth Asian Conference on
Ciliate Biology organized by Kalyani University, Kalyani, India.
➢ Renu Gupta, Seema Makhija, Ravi Toteja, Jeeva Susan Abraham and S. Sripoorna (2014) Oxytrichid
ciliate biodiversity from the river Yamuna, Delhi, India. 2nd
Asian congress of Protistology and Ninth
Asian Conference on Ciliate Biology organized by Kalyani University, Kalyani, India.
➢ Jeeva Susan Abraham, S. Sripoorna, Renu Gupta, Ravi Toteja and Seema Makhija (2015). Phylogenetic
relationships among the ciliates (class Spirotrichea) isolated from Indian Subcontinent with emphasis on
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the subclass Stichotrichia inferred from small subunit rRNA gene sequence. 4th
IRCN-BC Workshop held
at Qingdao, China.
➢ S. Sripoorna, Jeeva Susan Abraham, Renu Gupta, Ravi Toteja and Seema Makhija (2015)Taxonomic
evaluation of the genus Tetmemena sp. by morphological and multiple molecular markers to assess its
phylogeny. 4th
IRCN-BC Workshop held at Qingdao, China.
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➢ Ashish Choudhary, Manu Sankar, Vikas Ahlawat, Jeeva Susan Abraham, S. Sripoorna, Seema Makhija
and Ravi Toteja (2015). A low cost microbiotest for detecting insecticides by exploring behavioural and
ecotoxicological response of freshwater ciliates. Recent trends in Biology organized by Department of
Zoology, Maitreyi College, University of Delhi, New Delhi, India.
➢ Renu Gupta, Jeeva Susan Abraham, S. Sripoorna, Bhawana Kumari, Shivangani Lohani, Divya Ojha,
Ranjeeta Bhujbal, Seema Makhija and Ravi Toteja (2015). Elucidation of phylogenetic status of
Blepharisma sp. from Delhi, India: Congruence of classical and molecular markers. Recent trends in
Biology organized by Department of Zoology, Maitreyi College, University of Delhi, New Delhi, India.
➢ Shiv Jangra, Khushi, Simran, Sidhanth Zutshi, Bhupender Singh, Jyoti Dagar, Anit Kumar, Anusha
Goyal, Muskan Bhatnagar, Meenu Upadhaya, Jeeva Susan Abraham, S. Sripoorna, Ashish Choudhary,
Ravi Toteja, Seema Makhija, Geetu Gambhir and Renu Gupta (2016) A Study on physico-chemical
analysis of soil samples collected from various sites in and around Delhi. National Symposium on Man-
Made diseases: an urban menace organized by Department of Zoology, Maitreyi College, University of
Delhi, New Delhi, India.
➢ Shiv Jangra, Khushi, Simran, Sidhanth Zutshi, Bhupender Singh, Jyoti Dagar, Anit Kumar, Anusha
Goyal, Muskan Bhatnagar, Meenu Upadhaya, Jeeva Susan Abraham, S. Sripoorna, Ashish Choudhary,
Ravi Toteja, Seema Makhija, Geetu Gambhir and Renu Gupta (2016) Ciliate diversity as a cellular tool
to assess soil quality. National Symposium on Man-Made diseases: an urban menace organized by
Department of Zoology, Maitreyi College, University of Delhi, New Delhi, India.
➢ Shiv Jangra, Khushi, Simran, Sidhanth Zutshi, Bhupender Singh, Jyoti Dagar, Anit Kumar, Anusha
Goyal, Muskan Bhatnagar, Meenu Upadhaya, Jeeva Susan Abraham, S. Sripoorna, Ashish Choudhary,
Ravi Toteja, Seema Makhija, Geetu Gambhir and Renu Gupta (2016) Community characteristics of soil
ciliates collected from three different sites in Acharya Narendra Dev College, University of Delhi.
National Symposium on Environment: Greener Future and Awareness organized by Department of
Zoology, Deshbandhu College, University of Delhi, New Delhi, India.
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Research Projects (Major Grants/Research Collaboration)
Awards and Distinctions
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➢ Awarded the Best Oral Presentation for the research paper entitled “Inferring Phylogenetic Position of
an Indian Isolate Tetmemena sp. (Ciliophora, Oxytrichidae) Using Morphological, Morphogenetic and
Molecular Markers” at 2nd
Asian congress of Protistology and Ninth Asian Conference on Ciliate Biology
organized by Kalyani University, Kalyani, India (2014).
➢ Co-authored a paper awarded as the Best Poster Presentation entitled “Oxytrichid ciliate biodiversity
from the river Yamuna, Delhi, India” at 2nd
Asian congress of Protistology and Ninth Asian Conference
on Ciliate Biology organized by Kalyani University, Kalyani, India (2014).
➢ Honoured and presented with “DISTINGUISHED TEACHER AWARD” by University of Delhi
(2009).
➢ Council of Scientific and Industrial Research (CSIR)-Research Associateship (1999).
➢ German Science Federation - Indian Council of Medical Research fellowship to work in Germany
(1995).
➢ CSIR-UGC-NET for Junior Research Fellowship (1993).
➢ Graduate Aptitude Test Examination (GATE) (1993).
Association With Professional Bodies
Other Activities
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CONFERENCES/WORKSHOPS/SYMPOSIUM ATTENDED AND ORGANIZED
➢ Organized and Attended National Symposium on Biotechnology: Expanding Horizons organized by
Acharya Narendra Dev College and held at INSA auditorium (2003).
➢ Participated in the 5th
Annual Symposium on Frontiers in Biomedical Research held at Dr. B.R.
Ambedkar Centre for Biomedical Research, University of Delhi (2004).
➢ Attended the National Symposium Biohorizons 2004 organized by Indian Institute of Technology, Delhi
(2004).
➢ Attended National Seminar on Recent Trends in Chemistry held at Maitreyi College University of
Delhi (2004).
➢ Attended one day Workshop on Bioinformatics in the Department of Botany, North Campus, University
of Delhi (2005).
➢ Attended one day workshop on Delhi State Level Symposium on Forensic Science organized by
S.G.T.B. Khalsa College, University of Delhi (2005).
➢ Joint Secretary and also presented papers in the International symposium on Ciliate Biology
Biodiveristy, ultrastructure, development, genome and parasitism organized by SGTB Khalsa
College, University of Delhi (2007).
➢ Resource person in a ten day workshop Understanding complexities of life from molecules to system:
Concepts and tools for undergraduate students of Biology in the college (2008).
➢ Resource person in four day Biology Laboratory Course Workshop, B.Sc. Programme LSIII, organized
by Institute of Life Long Learning, University of Delhi and Acharya Narendra Dev College (2008).
➢ Participated in five day Wikied’09 Workshop, organized by Acharya Narendra Dev College and
CEMCA (2009).
➢ Preparation of online quizzes on Life Sciences subjects, organized by Institute of Life Long Learning
(ILLL), University of Delhi (2009).
➢ Part of the Organizing Committee of two day National Symposium on Chemistry in Biology: The
Future of Life Sciences organized by Acharya Narendra Dev College, University of Delhi at India
International Centre, New Delhi (2010).
➢ Participated in Three week workshop entitled “Easy Now - A Course Material Preparation Module”
held at Acharya Narendra Dev College. The course material is uploaded on website
http://eduframe.net/andc (2010).
➢ Participated in six day Easy Now Workshop on Multimedia Content Development, organized by
Acharya Narendra Dev College and CEMCA (2010).
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➢ Member of the organizing committee for organizing two day workshop on Hands on Training,
Molecular Phylogenetics: Understanding Evolutionary Histories, (Workshop for Students) on
organized in Acharya Narendra Dev College (2011).
➢ Attended five day workshop on Science Education and Research co-organized by Vidya Bhavan
Society and Acharya Narendra Dev College (2012).
➢ Attended one day seminar on Use of Animals in Education and Research held at Department of
Zoology, University of Delhi (2012).
➢ Coordinator of one day workshop on practical related to paper Evolutionary Biology to be held at SGTB
Khalsa College, University of Delhi (2013).
➢ Member of the organizing committee of 9th
Asian Congress on Ciliate Biology and 2nd
Asian Congress
of Protistology to be held at University of Kalyani, West Bengal (2014).
➢Resource person for two-day teachers‟ workshop for P.G.Ts in Physics, Chemistry and Biology entitled
Science Pedagogy: Laboratory Learning to Classroom Teaching at ANDC on January 20-21, 2016.
It was an endeavour to promote Science and impart hands-on training in modern techniques related to
subject curriculum to the school teachers.
CURRICULUM DEVELOPMENT
Member of committee involved in restructuring the course contents of Applied Biology and Biotechnology for B.Sc. (Life Sci.) III year.
EXTRA CURRICULAR ACTIVITIES
➢ Member of Social Outreach Committee and NSS
➢ Member of Editorial Committee for the College Magazine, Annual Report and Prospectus
➢ Member of Sashakt, a committee for female students. As a member of Sashakt, organized a self defense
programme for female students.
➢ Member of Finance Committee of the college
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