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168 Peptidoglycan in atherosclerotic plaque formation and vulnerability Manon Oude Nijhuis

Color Supplement

Figure 7. PG and Pam3Cys-SK4 increase macrophage homing into atherosclerotic plaquesTheabilityofmacrophagestomigrateintotheatheroscleroticplaqueuponPGandPam3Cys-SK4stimulationwasinvestigatedinApoE-/-mice.ControlGFP-macrophagescouldhardlybefoundintheatheroscleroticplaques(onlyafewcellsinthewholeaorticarch),whereasmacrophagesthatwerestimulatedwitheitherPGorPam3Cys-SK4werefoundeasier(afewmacrophagesineachatheroscleroticplaquewithintheaorticarch).TheleftpanelshowsGFPcells in thearterialwall, themiddlepanelshowsHoechstnuclearstainingandtherightpanelshowsthemergedpicture.

Chapter 7

169Peptidoglycan in atherosclerotic plaque formation and vulnerability Manon Oude Nijhuis

Figure 6. NOD2 is expressed by macrophages and endothelial cells in atherosclerotic plaquesDouble immunofluorescent staining for NOD2 (green) and cell markers (red) on atherosclerotic lesions. Cells were stainedforCD163 (macrophages)andvonWillebrandFactor (endothelialcells).Double-positivecellsareyellowin the overlay panel. Cell nuclei are stained blue with DAPI. Original magnification: left panel 400x, right panel 200x.

Chapter 8

Color supplement