color supplement - dspace.library.uu.nl · color supplement figure 7. pg and pam 3 cys-sk 4...

168 Peptidoglycan in atherosclerotic plaque formation and vulnerability Manon Oude Nijhuis Color Supplement Figure 7. PG and Pam 3 Cys-SK 4 increase macrophage homing into atherosclerotic plaques The ability of macrophages to migrate into the atherosclerotic plaque upon PG and Pam 3 Cys-SK 4 stimulation was investigated in ApoE-/- mice. Control GFP-macrophages could hardly be found in the atherosclerotic plaques (only a few cells in the whole aortic arch), whereas macrophages that were stimulated with either PG or Pam 3 Cys-SK 4 were found easier (a few macrophages in each atherosclerotic plaque within the aortic arch). The left panel shows GFP cells in the arterial wall, the middle panel shows Hoechst nuclear staining and the right panel shows the merged picture. Chapter 7

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168 Peptidoglycan in atherosclerotic plaque formation and vulnerability Manon Oude Nijhuis

Color Supplement

Figure 7. PG and Pam3Cys-SK4 increase macrophage homing into atherosclerotic plaquesTheabilityofmacrophagestomigrateintotheatheroscleroticplaqueuponPGandPam3Cys-SK4stimulationwasinvestigatedinApoE-/-mice.ControlGFP-macrophagescouldhardlybefoundintheatheroscleroticplaques(onlyafewcellsinthewholeaorticarch),whereasmacrophagesthatwerestimulatedwitheitherPGorPam3Cys-SK4werefoundeasier(afewmacrophagesineachatheroscleroticplaquewithintheaorticarch).TheleftpanelshowsGFPcells in thearterialwall, themiddlepanelshowsHoechstnuclearstainingandtherightpanelshowsthemergedpicture.

Chapter 7

Page 2: Color Supplement - dspace.library.uu.nl · Color Supplement Figure 7. PG and Pam 3 Cys-SK 4 increase macrophage homing into atherosclerotic plaques The ability of macrophages to migrate

169Peptidoglycan in atherosclerotic plaque formation and vulnerability Manon Oude Nijhuis

Figure 6. NOD2 is expressed by macrophages and endothelial cells in atherosclerotic plaquesDouble immunofluorescent staining for NOD2 (green) and cell markers (red) on atherosclerotic lesions. Cells were stainedforCD163 (macrophages)andvonWillebrandFactor (endothelialcells).Double-positivecellsareyellowin the overlay panel. Cell nuclei are stained blue with DAPI. Original magnification: left panel 400x, right panel 200x.

Chapter 8

Color supplement

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