central dogma dna is made (transcribed) into rna rna is made (translated) into protein
Post on 18-Dec-2015
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Central dogma
• DNA is made (transcribed) into RNA• RNA is made (translated) into protein
DNA is made into RNA
• A protein enzyme, polymerase, transcribes (makes) DNA into RNA.
You will transcribe DNA into RNA today!
RNA made into protein
RNA is genetic material that codes for proteinRNA must contain the correct code to make the correct protein
Ribozyme
• RNA that acts as an enzyme• Enzymes speed up a reaction• Ribozymes cleave (cut) RNA
Splicing
Splice – to join together at the endsGenes can be cut and pasted together (spliced!)
Why Splice?Exon 1 Intron Exon 2
Certain parts of RNA do not code for proteins,
they need to be cut out
Exon – part of gene that codes for proteinIntron – does not code for protein, splice it out!
Splicing Before splicing
Exon 1 Intron Exon 2
After splicing
Exon 1 Exon 2
Exon –codes for proteinIntron – does not code for protein, splice it out!
Splicing Before splicing
Exon 1 Intron Exon 2
After splicing
Exon 1 Exon 2
Exon –codes for proteinIntron – does not code for protein, splice it out!
Ready for Protein! Before splicing
Not ready to be made into protein
After splicing
Ready to be made into protein
Exon –codes for proteinIntron – does not code for protein, splice it out!
Ribozyme
• Ribozymes can splice RNA together
You will be splicing RNA together using a ribozyme today
What will you do?
• You will transcribe DNA into RNA. This will be done by incubating the DNA with polymerase, the enzyme that turns DNA into RNA.
What will you do?
• The RNA that you will make contains a ribozyme. The ribozyme will splice itself! Each group will transcribe and splice for a different amount of time.
Group 1- 5 minutesGroup 2 – 10 minutesGroup 3 – 15 minutesGroup 4 – 20 minutes
What will it look like?
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