betula uber

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 1/17

JORESSIA A. JAMISON AND MICHAEL H.RENFROE

Department of Biology, JamesMadison University, Harrisonburg,

Virginia 22807 

(Received 21 March 1997; accepted23 January 1998; editor T. A. Thorpe)

MICROPROPAGATIONOF BETULA UBER 

MikropropagacijaBetule uber 

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 2/17

Virginijskaokroglolistna

breza

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 3/17

GOJIE

Package SizePrice

($USD)

1L $1.25

10L $8.2550L $27.00

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 4/17

+ hormoni (citikinini)

+

 Autoklaviranje121°C1,05kg/cm215 min

8 g/L DifcoBocto agar 

1M 5M 10 M

- 6 benzilamino adenin (BA) ali

- 2-izopentenil adenin (2iP) ali

- kinetn

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 5/17

IZSEEK 4 - 6 SADIK(14,5 ± 64 cm)

Pomlad, poletje,

Mlado drevo,dobro razvejano

Sterilizacija

(15% Clorox 15 min)

7× destilirana voda

Nodije obreemo

-pokodovano tkivo

Kozarki

(babyfood jars)

Petrijevka

SEGMETI z

3 ± 4 nodiji

Spiranje izseka

Tween 20 (5 min)

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 6/17

 RASTN  E KOMO R E

Dnevni cikel

28°C

15 ur (6:00-21:00)

Noni cikel

18°C

9h (21:00-6:00)

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 7/17

BUD BREAK

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 8/17

FIG. 1. Comparison of bud break of Betula uber 

nodal explants over timestimulated by 2-iP (A), kinetin (B), and BA ((2) atdifferent concentrations.Each point represents the results from 20 cultureswith one explant per culture.

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 9/17

FIG. 2. Comparison of mean shoot elongationof Betula uber nodal explantsat 4 wk stimulated by BA, 2-iP, and kinetin atdifferent concentrations.Each point represents the results from 20cultures with one explant per culture.

Basalcallusing ±bazalni kalus34% najve

Vitrifikacije NI

Temno zelena rastlina

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 10/17

FIG. 3. Comparison of Betula uber bud break over time stimulated by BAat different concentrations, or BA in combination with GA 3. Each point 

representsthe results from 20 cultures with one explant per culture.

FIG. 4. Comparison of mean shoot elongationof Betula uber nodal explantsover time stimulated by BA at differentconcentrations, or BA in combinationwith GA 3. Each point represents the resultsfrom 20 cultures withone explant per culture.

Preverjanje konc. Okrog 10M BA (7,5 M 10 M 12,5 M)+ 2,9 M GA

WPM + BA so neznatnomanj brstele (4 tedni)

WPM + BA in GA dobro

brstenje

1,9 cm

0,325cm

WPM + BA + GA

- bazalni kalus (44%)

- stekleni listi

WPM + BA

- ni steklenih listov

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 11/17

POGANJEK 1,5 CM

 P R ESA J AN JE I N  KO R EN  I N JEN JE

ZEMLJA

Promix/perlite

WPM + BA + GAslabo preneslepresajanje

WPM + BA

Dobro preivelepresajanje

77,8%

Najbolje ukoreninjenje WPM + 12,5 M BAGojie brez hormonov neuspenokoreninjenje

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 12/17

 RAZ I S K  AV  E /  ANAL I  Z E

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 13/17

 RAZ I S K  AV  E /  ANAL I  Z E

Polisadnimezofil(parenhim)

Debelinapovrhnjice intrihomi

Gostota in velikostlistnih re

SEM in vrstini elektronski mikroskop

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 14/17

 RAZ I S K  AV  E /  ANAL I  Z E

- po 1 tednu v in vitro

- po fazi prenosa

- v zemlji ( 2. teden)

- po aklimatizaciji

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 15/17

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 16/17

FENOTIPSKESPREMEMBE

8/3/2019 betula uber

http://slidepdf.com/reader/full/betula-uber 17/17

S K  L E P I 

Mona mikropropagacija Butule uber  Gojie WPM z BA in GA nam daje najhitreje brstenje

Betule uber ± giberelinsko odzivna vrsta Aklimatizirane rastline imajo debelejo kutikolo Na podlagi re smo ugotovili FENOTIPSKI ODZIV kiomogoi preivetje rastlin glede na razmere

Te tehnike se lahko izkoristi za uporabo propagacije ostalihlistavcev ki so e ogroeni poleg Betule uber