ACCELERATING INNOVATIONSRESEARCH FACILITIES AND SERVICES

38 MINUTES FROMSCHIPHOL AIRPORT

6 KM FROM UTRECHTCENTRAL STATION

IMPROVING HEALTH AND QUALITY OF LIFE

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STRATEGIC THEMES

Utrecht Science Park provides a vibrant, dynamic and exciting place towork, to study and to interact. We bring competences from business,industry and academia together in order to design and create healthier,safer and more sustainable cities for today and for subsequent generations.We have combined our core competences into strategic themes in thedomains of Life Sciences & Health, Sustainability, and Applied Gaming tosupport this ambition (see graph on opposite page).

For more information on the promise of the Utrecht Science Park forsociety, please refer to our brochure ‘Improving Health and Quality of Life’.

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THE BEATING HEART OF EUROPE’S MOST COMPETITIVE REGION

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80knowledge-driven

organisations

300hectares

1,200,000,000euro investment

until 2018

50,000 students22,000 employees

21% of the amount of people living

in Utrecht

DAILY:

72,000 people

on 3%of its area

A12

A27

A28

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LAN

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A27

A28

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EMPLOYEES

22,000

STUDENTS

50,000 5,000

PATIENTS RECEIVE MEDICAL TREATMENT

EVERY YEAR

400,000

STUDENT HOMESWITH A POTENTIALTO GROW TO 4,000

NEW JOBS IN 5 YEARS

2,500

Utrecht Science Park is home to Utrecht University,University Medical Center Utrecht, University ofApplied Sciences Utrecht (Hogeschool Utrecht), andseveral research institutes and research companies.These institutions of basic and applied research,education and clinical care with top researchers andtalented students make the Utrecht Science Park anattractive location for innovative researchcompanies because of the completeness of theecosystem. In addition to excellent educationalprograms they have a unique researchinfrastructure that is among the best in Europe. Inrecent years, for approximately € 60 million wasinvested in expanding the research infrastructure.This makes the Utrecht Science Park an excellentplace where scientists from research organizationsand science-based companies can accelerate theirinnovations.

To unlock the unique and rich ecosystem of talent,expertise and research infrastructure and tohighlight the research and trainee opportunities, wehave initiated the BOOSTER program. The purposeof this program is to streamline the delivery of

cutting-edge infrastructure to the researchcommunity at large using a central front office.Startup companies, small- and medium enterprises,as well as corporations, that are in need of researchinfrastructure or trainees do not need to find theirway in the complexity of different organizations anddepartments but can contact us with their request.We will help you to connect to the right facilities orpersons that can answer your question and toaccelerate your innovation. If necessary we canprovide you with integrated solutions comprisingmultiple facilities and services. At the same time weassist the research and educational institutions byproviding exposure for their facilities and traineesand by supporting the collaboration withknowledge-intensive companies. To this end, wework closely with business development managersof the several institutes and departments.

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Front office

Establihing

Research

Companies

Public

Private

Partnership

iLab

Education,

Training &

Internship

Research

Facilities

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IF YOU ARE INTERESTED IN ANY OF THE REFERENCED FACILITIES ORSERVICES PLEASE CONTACT researchfacilities@utrechtsciencepark.nl.

Together with our science partners, we provide awide range of facilities and services for pre-starters,startups, scale ups, and mature companies. From asingle, professional and customer-oriented frontoffice, we support startup and scale up companies

to develop and grow their ventures, and we haveservices for established companies and largercorporates that help them to connect to the richecosystem of the Utrecht Science Park (see graphbelow).

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Accommodation

Seed Startup Scale up Mature

Talent, Training & Support

Funding & Financing

Research Infrastructure & Expertise

Connecting & Market Access

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The Utrecht region is a very attractive location for(inter)national organizations and companiesbecause of its central location in the Netherlandsand in Western Europe, the proximity of AmsterdamAirport Schiphol and the high educational level ofthe population. Many leading internationalcompanies, knowledge institutes and researchcenters can be found in Utrecht. Utrecht SciencePark in particular is an attractive location forstartups and research companies working in thefields of life sciences, health and sustainability. We welcome organizations and companies that are(willing to) work together with our researchinstitutes to establish their research activities at the Utrecht Science Park.

There are several possibilities to establish yourcompany at the Utrecht Science Park, depending on your requirements and type of activities.

- Pre-starter and temporary facilities- Shared facilities for startups and scale-up

companies- Shared facilities for scale-up and mature

companies- Independent premises for mature companies

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ESTABLISH YOUR COMPANY AT THE UTRECHT SCIENCE PARK

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ILAB UTRECHT

Co-creationiLab (innovation Lab) Utrecht is a fully equipped andstaffed laboratory in which companies have accessto research infrastructure, students and scientists ina co-creation setting. The first iLab Utrecht facilitywill be accommodated by the University of AppliedSciences; we expect to open multiple iLabs in otherlocations at the Utrecht Science Park that provideadditional facilities and fields of expertise. Thecollaboration with research and education institutesenables companies to work in a researchenvironment without the need for heavyinvestments to establish their own labinfrastructure. Furthermore, the access to studentsenables companies to identify talented studentsand offer an internship or even an employmentcontract. For startup companies that are still in theprocess of attracting financing for their venture, wecan develop tailor-made business models forpublic-private partnerships. iLab Utrecht is part ofan ecosystem strategy that will organize not only

access to infrastructure and human capital but alsoaims to facilitate start-ups in other ways, byproviding incubation and acceleration programs.

National networkiLab Utrecht in its current form has expertise andfacilities in specialized chemical analysis, chemicaltechnology, molecular & cell biology, microbiologyand alternative-to-animal-tests. However, iLabUtrecht is part of a national network of iLabs that isestablished by the Top Sector Chemistry. Currentlythere are 10 locations where an iLab is eitherestablished or under development. Hence, yourresearch question can be passed on to a wideexpert network in (bio)chemistry.

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ADEQUATE FACILITIES FOR COMPANIES IN LIFE SCIENCES FROM STARTUP TO MATURITY

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Life Sciences IncubatorThe newly build Life Sciences Incubator can hostinnovative startup and scale up companies that areactive in the life sciences and require both officeand lab space for their activities. The Life SciencesIncubator is hosted by Stichting Incubator, a legalentity fully owned by Utrecht University.

The facility comprises 4000 m2 effective floor areawith offices, laboratory spaces and general facilitiessuch as reception and meeting facilities. Thebuilding enables a community of life sciencesresearch companies that can benefit fromsubsidized rent and startup support. The building isdirectly connected to an accelerator building for lifesciences companies.

The Life Sciences Incubator is the first laboratorybuilding in The Netherlands with a sustainabilityrating of BREEAM Outstanding*. The Life SciencesIncubatoris supported by the European RegionalDevelopment Fund.

Accelerator buildingResearch companies and corporate researchorganizations that require larger volumes of officeand lab space can be hosted in the separateaccelerator building. In addition, Utrecht SciencePark is preparing for the development of a newaccelerator building that can also host companiesrequiring larger volumes.

In case you are interested in establishing your ownfacility at the Utrecht Science Park, there are severalbuilding plots available for land lease.

*BREEAM (Building Research Establishment Environmental Assessment Methodology) is the world’s longest establishedmethod of assessing, rating, and certifying the sustainability of buildings.

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TALENT ANDSTARTUP SUPPORT

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INCUBATOR UTRECHTINC

Startup incubator UtrechtInc at the Utrecht SciencePark supports alumni, scientists and entrepreneursto successfully set up (IT) companies. Together withthese smart entrepreneurs in the fields ofhealthcare, healthy environment and education,UtrechtInc makes a positive impact on society.

UtrechtInc ranks the 6th best incubator in Europeand has supported over 135 startups to date. Inaddition to the accelerator programs, UtrechtIncalso offers access to financing, office space,coaching, experts and a startup community. In theprogram the startups work at an accelerated paceto develop a working product, secure payingcustomers and scale their business model. Thestartups flow in from Science Venture or PressureCooker to Build.

Programs1. Science Venture: startup program (6 months) for

scientists who take the first steps to bring ideas,discoveries or research to market.

2. Pressure Cooker: startup program (4 months) forentrepreneurs who have designed their businessmodel and first prototype.

3. Build: customized support (±1,5 year) forentrepreneurs who have successfully completedScience venture or Pressure cooker.

Corporate InnovationUtrechtInc also helps corporate innovationteam(s) to bring new innovations to market andfoster a culture of entrepreneurship within theexisting organization. Corporates are offeredstartup expertise and learn how to innovate withthe speed and agility of startups. 

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EXECUTIVE MASTER OF BUSINESS INNOVATION &ENTREPRENEURSHIP LIFE SCIENCES & HEALTH

The Executive Master of Business Innovation &Entrepreneurship in Life Sciences & Health (MBI LifeSciences & Health) is a unique ‘on-the-job’ MBAdevelopment program to accelerate your venture or

business case on a global scale. It acknowledges thatonly after the initial business plan real challengesemerge and it empowers entrepreneurs to take theirventure to the next level and transform their venture

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into a sustainable growing company. The program isan initiative of Dutch, Belgian and North-Americantop-league health and research institutes includingUMC Utrecht, Utrecht University, VIB (VlaamsInstituut voor Biotechnologie) and FlandersBio. It isfacilitated by Julius Academy and supported by anactively involved international Advisory Board with aproven entrepreneurial track record.

What MBI LS&H offers:- Modules: State of the art knowledge on

entrepreneurship, innovation strategy, businessmodel innovation, risk management, andbusiness valuation taught by world-leadingexperts, investors and entrepreneurs.

- Entrepreneurial labs: Apply it to your casethrough performance assessment, building agrowth strategy and action plan throughindividual guidance by experiencedentrepreneurs and develop a more mature andviable business case to scale your businessfaster.

- Leadership development & reflection: Developentrepreneurial leadership skills, improvenegotiation skills, alpha sales skills and corporatemarketing skills. Be effective in collaboration andcommunication with stakeholders across andoutside your organization.

- Global network: Deepen and enlarge yourglobal network with a relevant scientific, investorand business community within the Life Sciences& Health domain.

Accelerate your venture toa global scale

2020

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ACADEMIC TRAINEES

InternshipsUtrecht University each year has 10,000 Masterstudents and 6,000 final year Bachelor studentsthat are eligible for internships. Utrecht Universitywants its students to be well prepared for thetransition to the labor market. Internships play animportant role in training students and providingthem with the necessary knowledge and skills thatare required in their future profession.

For companies and organizations, offeringinternship positions is a useful way of getting toknow young talent and selecting new recruits forjob openings.

Career ServicesCareer Services provides services to students,young alumni and PhD students in the form of jobinterview training, CV checks, assessments, aninternship bank, a job board and information aboutcareer opportunities. The program includesworkshops, training sessions, companypresentations, opportunities for one-to-oneconversations and a company fair. The annualCareers Day offers your company the opportunityto meet our students directly.

Career Services is very interested to get in touchwith companies and organizations who offerinternship positions on a regular basis to establish asustainable relationship, for example in the form ofa partnership or a framework agreement.

Huge potential of 16,000 students for internships

RESEARCH INFRASTRUCTURE

FROM MOLECULE TO POPULATION

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NMRLarge Scale

Facility

NPC Proteins at Work

Proteomics Facility

LipidomicsFacility

UtrechtMonoclonalAntibodyFacility

National Single Crystal X-Ray Facility

AbLabNative

Protein MSServices

UtrechtSequencing

Facility

Utrecht Nanobody

Facility

MedicalMeta-

bolomicsFacility

BiologyImagingCenter

ElectronMicroscopy

Square

Center for Cell Imaging

UtrechtCell

MicroscopyCore

molecular cellular

Utrecht Life Sciences is anopen innovation network thatfacilitates strategic public-private partnerships ineducation, research andbusiness. Our main goal is todrive innovations in humanand veterinary health care.We create shared value forour partners whilesimultaneously providingeconomic and societalbenefit.

For further information visitour website atwww.utrechtlifesciences.nl.

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FlowCytmetry

and Cell Sorting

Facility

CellScreening

Core

DutchMolecularPathology

Centre

Laboratoryfor

MedicalMicro-biology

MedicalDevice

Prototyping Facility

LargeParticleFlow

CytometryFacility

Single CellAnalysisCenterUtrecht

DigitalHuman

Pathology

CBS-KNAWFungal

BiodiversityCentre

ScientificInstru-

mentation

3Rs-CentreUtrecht

LifeSciences

FacilitiesDepartment

of FarmAnimalHealth

PharmacyLaboratory

UtrechtBio-

fabricationFacility

BiomedicalMR Imaging

&Spectroscopy

Facility

JuliusSupport

forResearch& Trials

MultiplexCore

Facility

HybridOperationRoom for

LargeAnimals

UtrechtPatientOrientedDatabase

UtrechtCenter forDiagnosticAdvances inImmunology

Research

UtrechtBio-

informaticsCenter

High-Throughput

ELISAFacility

organ & tissue organism technicalclinical

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FACILITIESFOR

MOLECULARRESEARCH

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spectral sensitivity. The NMR facility also functionsas an European computational e-Infrastructure forNMR and structural biology. The computationalinfrastructure consists of several linux clusters withover 500 CPU cores. It also provides access to GRIDcomputing. Further information can be obtainedfrom the web site.

AccessThe Utrecht Facility is a national and Europeanresearch infrastructure for solution and solid-stateNMR, as well as computational structural biologywith access for local, national and internationalresearchers.

NMR LARGE SCALE FACILITY

Novel research concepts are being developed andapplied to complex biomolecular systems includingprotein-protein and protein-nucleic acid complexesor membrane proteins. Additional research isconducted on heterogeneous systems such ashydrogels, biomaterials, liposomes and cellularmembranes. The facility is part of the Bijvoet Centerfor Biomolecular Research.

Specifications main equipmentAs a national and European large scale facility,state-of-the-art instrumentation for NMRexperiments on soluble molecules orheterogeneous preparations (solid-state NMR) isavailable. Currently, NMR fields range from 500 MHzto 950 MHz (solution NMR) and from 400 MHz to950 MHz (solid-state NMR). In addition, two solid-state spectrometers (400 and 800 MHz) areequipped with gyrotrons for DNP (dynamic nuclearpolarisation), which provides highly increased

Gaining fundamental insightinto molecular processes

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NATIONAL SINGLE CRYSTAL X-RAY FACILITY

With our experienced staff, state-of-the-artequipment and advanced software we are able tohandle a large variation of chemical compounds. Inacademic collaborations we contribute significantlyto the preparation of scientific publications.Examples of our structural studies include modelsystems that mimic catalytic sites in proteins orsynthetic catalysts to be used in the cleanproduction of desired pharmaceuticals. Forenantiopure compounds we are able to determinethe absolute configuration, even in the absence ofheavy atoms.

Our modern equipment for protein crystallographyenables us to perform screening experiments forprotein crystallization and to optimize the results.Co-crystallization and soaking of pharmaceuticalfragments is possible. The protein structuredetermination can be performed in-house.

Equipment- Bruker Kappa Apex II diffractometer- Bruker Proteum diffractometer- Low temperature crystal mounting- Small-volume protein crystallization robot

AccessAccess is available to universities, institutes andcompanies in the Netherlands.

We offer crystal structuredeterminations to synthetic

chemists at universities,institutes and companies

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Large scale analysis of proteinsand their interactions in relation

to biological functions

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NPC PROTEINS AT WORKPROTEOMICS FACILITY

The Proteins At Work facilities of the NetherlandsProteomics Center provides access to state-of-the-artproteomics technology, equipment and expertise toresearchers in the Life Sciences from academia andindustry. The core facility at Utrecht University is anexpert center for state-of-the-art proteomics analysisthat develops and implements innovative massspectrometric methods for efficient and detailedcharacterization of biomolecules in relation to theirbiological function. The facility offers massspectrometry based proteomics technology forapplications ranging from single proteinidentifications to the analysis of complex, whole-celllysate, samples and determination ofposttranslational modifications.

Equipment and accessThe instrumentation of the facility consists of a widerange of HPLC coupled high-end mass spectro-meters, including state-of-the-art technologies such

as high-resolution Orbitrap mass spectrometers withCID, HCD and ETD fragmentation capability. For datastorage and analysis, the facility has dedicatedcomputational clusters. The facility offers its servicesthrough research collaborations with scientists in theNetherlands, Europe and world-wide.

NPC Out-stationThe proteomics facility at the University MedicalCenter Utrecht is an out-station of the Proteins atWork initiative of the Netherlands ProteomicsCenter. The location of the facility within theDepartment Molecular Cancer Research, part of theCenter for Molecular Medicine, ensures a strongbackground in biochemistry and molecular biology.Together with the high-end equipment, this allowsfor tailor made innovative approaches to aidfundamental, translational and clinical research intoproteins and their modifications relevant for healthand disease. Questions that are addressed rangefrom single protein identification to proteomequantification. The facility offers its services primarilyto researchers from the UMCU and its partners.

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ABLAB NATIVE PROTEINMASS SPECTROMETRYSERVICES

AbLab is the service unit that operates within theUtrecht University, providing state of the art nativemass spectrometry services.

Native MSNative mass spectrometry (native MS) allows themass spectrometric analysis of intact proteins andprotein complexes under their native folded stateproviding a wide range of information, such asstructural information, complex stoichiometry andsample composition and purity. The key feature thatmakes native MS unique is the retention ofmolecular non-covalent interactions during theionization process and the subsequent massanalysis. Over the years, the progressivedevelopment of this analytical technique has beenpushing the limits of this technique, today allowingthe measurement of large, intact protein complexes.

EquipmentAblab can provide access to analysis of proteinsamples using dedicated, modified (Q)-ToF massspectrometers analysis, all providing information onthe structure and composition of the native proteincomplexes. The presence of an Orbitrap analyzerspecifically developed for intact protein analysisenables in-depth analyses of post-translationalmodifications (PTMs) directly at the intact proteinlevel.

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State of the art native massspectrometry services for

analysis of intact proteins andprotein complexes

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LIPIDOMICS FACILITY

The Lipidomics Facility houses chromatographic-and mass spectrometric equipment that isspecialized in the analysis of lipids. It providescomplete molecular analyses of a broad spectrumof (phospho)lipids (lipid fingerprinting) in thepicomole range as well as analysis of unusual lipids(lipid biomarkers).

High-throughputHigh throughput technologies have been developedallowing automated lipid analysis of 300samples/day. In combination with molecularimaging and modern information technology thiswill revolutionize our understanding of the complexinteraction networks in a functioning cell and howlipids together with genes and proteins determinecellular functions in health and disease.

EquipmentThe available technologies include MS basedlipidomics techniques (three mass spectrometers,all equipped with ion sources (turbo-)ESI, nano-ESI,and APCI, autosamplers, micropumps, UV detectors,UPLC and variable wavelength Fluorescencedetector), assays for lipid metabolism and lipid fluxusing heavy isotope labeled precursors, lipidisolation and separation techniques.

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Your partner for lipid analysisand fingerprinting

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UTRECHT SEQUENCINGFACILITY

ServicesWe are committed to support your Next GenerationSequencing (NGS)-based research by providingexpertise input for your experimental design, choiceof sequencing platform, advice on samplepreparation protocols, and help with data analysis.For experienced users, we provide sequencingcapacity and routine bioinformatic data analysis.The Utrecht Sequencing Facility (USF) is embeddedwithin an active research environment aiming at theuse of NGS technology beyond the current state-of-the-art. Extensive expertise is available with wet-laband bioinformatic aspects of whole genomesequencing (WGS), whole exome sequencing (WES),targeted sequencing, RNA sequencing (RNA-seq),amplicon sequencing (Ampli-seq), ChromatinImmunoprecipitation sequencing (ChIP-seq), andother.

EquipmentA range of DNA sequencers, liquid handlingrobotics, bioinformatic tools, and ICT infrastructureis available. We currently (Oct 2015) operateHiSeq2500, NextSeq500, MiSeq, Xten and OxfordNanopore MinION.

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Your partner for next-generation DNA

sequencing-basedexperiments

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UTRECHT CENTRE FOR MEDICAL METABOLOMICS

MetabolomicsIn metabolomics, one of the platform sciences ofthe various ‘omics’, the small-molecule complement(metabolome) of a biological system is identifiedand quantified. These diverse molecules, calledmetabolites, underpin the ongoing biochemicalreactions in a living cell, tissue and organism.Measurement of some individual metabolites iscommon practice for diagnostic purposes. Progressin analytical science and informatics allows theanalysis of a large part of the metabolomesimultaneously, providing not only information onthe individual metabolites but on the whole offunctioning biochemical networks.

The Utrecht Centre for Medical Metabolomics(UCMM) is a joint initiative of the department ofMolecular Cancer Research) and the department of

Metabolic Diagnostics of the UMC Utrecht. It unitesmetabolic knowledge from both the diagnosticlaboratory and more fundamental metabolicresearch. The UCMM is specialized in the study ofmetabolites in individual cells, tissues, and bodyfluids in context of biological perturbations andnutritional status. The centre has a variety of massspectrometry setups and develops and validatesboth targeted and untargeted assays that willbecome accessible as a fee-for-service forresearchers. At this point several metabolite assaysfor biomedical research can be offered.

Equipment- 2 Waters Xevo- Thermo Q-Exactive Plus- Thermo LTQ-Orbitrap-XL- Agilent 7000 GC-MS- Various U(H)PLC instruments (Waters Acquity,

Thermo Acella)- Nano-LC (Eksigent, Agilent)- Advion Triversa Nanomate (direct-infusion MS)

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ANTIBODY FACILITYUMAB

Monoclonal antibodies are important for specificbasic research, diagnostics and therapeutics. Thefacility offers:- Immunization of mice, wild type or knock out- Screening of serum titers- Generation and screening of hybridoma’s- IgG2c antibodies

New Immunization methodWe offer a new and very efficient cellularimmunization method. With this method,antibodies can be raised against low immunogenicmembrane proteins, such as multispanningmembrane proteins, conformational epitopes andwe can generate agonistic and antagonistic MABs.On request we can make MABs against solubleproteins/peptides with Complete Freund’s adjuvant.

Procedure/Services UMAB- DEC approval- Handling of mice- Collecting spleen of positive mice- Fusion of spleen cells to generate hybridoma’s- Selection of positive hybridoma’s- Long term storage

Extra services like antibody purification on request.

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Your partner for the selection andproduction of nanobodies

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UTRECHT NANOBODY FACILITY

NanobodiesAnimals of the camelidae family have next to their repertoire of conventional antibodies also smaller antibodies,which are composed of heavy chain only and are devoid of light chains. These unusual small sized antibodies can be further reduced in size in absence of the Fc region. This structure is called single domain antibody (sdAb),variable domain of the heavy chain antibody (VHH) or nanobody. Nanobodies belong to the smallest functionalantigen binding fragments (around 15 kDa), which are only one tenth the size of a conventional antibody.

ServicesNanobodies that bind to any possible target can be selected using phage display technology. The facility isexperienced in further functionalization of the nanobodies using different conjugation strategies includingsortase tagging and click-chemistry. Examples exist for application of different nanobody conjugates forsuperresolution light microcopy, electron microcopy, and for development of novel diagnostic and therapeuticapproaches including targeted nanoparticles and photodynamic therapy.

Activities- Llama immunization- Library production- Selection- Characterization- Functionalization- Production

Main equipment- AKTAxpress and AKTA prime - Fermentor BioFlo 115- BioFlo 115- Odyssey- Pearl imager- Light and Electron microscopes

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ELECTRON MICROSCOPYSQUARE

ServicesWhile classically electron microscopy (EM) allows forthe analysis of structures in the nanometer tomicrometer range, very recent advances in detectortechnology have brought EM into the field ofanalyzing biomolecules and nanomaterials at theatomic level. The EM Square at Utrecht University, a joint initiative between the faculties of Science,Geosciences and the UMCU is devoted to thedevelopment and application of technologies for(cryo) specimen preparation, 3D(cryo) electronmicroscopy data collection, as well as imageanalysis for cell biology and material sciencesapplications.

EquipmentThe infrastructure includes state-of-the-artTransmission Electron Microscopes (TEMs) andScanning Electron Microscopes (SEM), with a full

range of possibilities such as STEM, EDX andenergy-filtering. In addition it houses a specializedfocused ion beam SEM. The facility also hasequipment for specimen preparation withpreservation of ultrastructure (including cryo-fixation and freeze-substitution of samples),immunolabeling, serial-sectioning and high-resolution electron tomography and Slice and Viewto study three dimensional structure of cells, tissuesand other materials.

Electron microscopy for lifeand material science

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FACILITIES FORCELLULAR RESEARCH

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BIOLOGY IMAGING CENTER

ServicesThe Biology Imaging Center (BIC) of UtrechtUniversity provides access, support and training inadvanced light and fluorescent microscopytechniques. The experts at the Center have uniqueexpertise in fast multicolour imaging of intracellulardynamics in different types of mammalian cells,including neurons and cancer cells cultured in 2Dand 3D. The combination of single moleculebiophysics and strong cellular expertise, whichmakes it possible to perform analysis of the samemolecules in vitro and in vivo, is unique. The Centeralso has excellent expertise in laser microsurgery ofcells in application to mammalian cell biology andneurobiology research.

EquipmentThe Biology Imaging Center incorporatesequipment and expertise on Phase Contrastmicroscopy, VE-DIC microscopy, regular Wide-Field

Epifluorescence microscopy, Total InternalReflection Fluorescence (TIRF) microscopy, LaserScanning and Spinning Disk Confocal microscopy.The advanced techniques in which the Centercurrently specializes include multicolor live cellimaging with a high spatial and temporal resolution,3D live imaging of thick samples using Spinning Diskand Two-Photon microscopy, FluorescenceRecovery after Photobleaching (FRAP),photoactivation, photoablation, super-resolutionlocalisation microscopy (PALM/STORM) andStimulated Emission Depletion (STED) microscopy.

Visualizing cells, subcellularstructures, biological molecules

and their interactions

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CENTER FOR CELL IMAGING (CCI)

ServicesThe Center for Cell Imaging (CCI) is an advancedmicroscopy facility that specializes in digital imagingof diverse biological samples. We provideequipment and expertise in histology specimenanalyses as well as high-end fluorescencemicroscopy such as live cell confocal microscopyand super-resolution fluorescence microscopy.

The CCI allows preparation of biological samples inour wet-lab and has the expertise to efficientlyextract high content image data from them. Ithouses cryostats to make histology samples and adedicated digital microscopy setup for theiranalysis. The CCI provides detailed knowledge onhigh end fluorescence microscopy such as confocalmicroscopy, live cell microscopy, (intravital)multiphoton microscopy and super resolutionmicroscopy (by both structured illumination (SIM)

and single molecule light microscopy (SMLM)). CCIequipment for live cell imaging is positioned withina ML-II biosafety environment.

Equipment- Leica SPE-II confocal microscope (DMI4000)- NIKON Ti-E multifunctional microscope equipped

with high power laser and LED light sources. - GE-Healthcare OMX-V4 Blaze superresolution

microscope (SIM) equipped with laser and LEDlight sources.

- Zeiss LMS880 NLO Multiphoton microscope forintravital imaging

- Olympus BX60 upright microscope for histologyand fluorescence

- Leica S6D preparation microscope- Leica CM3050 cryostats- Several workstations for image analysis

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Your partner for advanced light-electronmicroscopy based experiments

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UTRECHT CELL MICROSCOPY CORE

ServicesWe are committed to support all your researchprojects that require microscopy. We provide expertinput for your experimental design, choice ofmicroscope, advice on sample preparation andprobe selection, and help with data acquisition andanalysis. We perform pilot studies and train peopleon site. On an annual basis we organize technicalcourses for novices in electron microscopy and forexperienced users we offer advanced Workshops inCorrelative Light - Electron Microscopy.

Infrastructure and equipmentThe Cell Microscopy Core (CMC) is an internationallyrecognized expert microscopy lab embedded withinthe active research environment of the Center forMolecular Medicine (CMM) of the University MedicalCenter (UMC) Utrecht. Our infrastructure comprises

equipment for fluorescent microscopy, live cellimaging and transmission electron microscopy.Together this comprises the full range ofmicroscopy methods for studies at the subcellular,cellular and tissue level. In addition, we arespecialized in immuno-electron microscopy,correlative light - electron microscopy (integratinglive cell imaging or light microscopy with electronmicroscopy on a single sample) and 3-dimensionalelectron microscopy.

We are committed to supportall your research projects that

require microscopy

5454

Courtesy and © Becton, Dickinson and Company. Reprinted with permission.

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FLOW CYTOMETRY ANDCELL SORTING FACILITY

Flow cytometryFlow cytometry literally means the measurement ofcells in flow. In real life it means that any suspensionof particles within the size range of approximately 50micrometer to 200 nanometer can be measured.The measurements can be performed at high speed,on average up to 20.000 per second. In this way datacan be obtained from each individual particle andhigh numbers of particles can be measured in ashort period of time. This technique is extremelysuitable for measuring sub-populations (e.g. of cells)within a suspension. In addition, individual cells orparticles can be purified using cell sorting.

ServicesThe Flow Cytometry and Cell Sorting Facility at theFaculty of Veterinary Medicine has accumulatedspecific expertise on measurement and sorting ofcells since 1998. This expertise builds on theexperience accumulated with a wide variety of

analyses and purifications, for example with immunecells, stem cells, sperm cells, chromosomes, bacteria,hybridoma’s for monoclonal antibody production,single cell sorting for cloning and/or PCR analysis,cell selection based on fluorescent reporter geneproducts and rare event purifications. We are trainedto rethink seemingly impossible demands intosuccessful experiments. The facility is activelyinvolved in research related to improvement ofresolution and is for this purpose in close contactwith the manufacturers of the equipment.

- For analysis of suspensions we can accommodateexperiments requiring lasers with the followingwavelength: 405 nm, 488 nm and 640 nm.

- For purification we can accommodate experimentsrequiring lasers with the following wavelength:Ultra Violet, 405 nm, 457 nm 488 nm, 561 nm and640 nm

- For sorting, up to 4 populations can be selectedsimultaneously in one run. There is a choice ofcollection modules like multi-well trays, tubes orslides where cells can be directly sorted in.

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LARGE-PARTICLE FLOWCYTOMETRY FACILITY(LPC)

Selection and purification of specific chemical orbiological materials are critical and often time-consuming aspects of fundamental and biomedicalresearch. Fluorescence-Activated Cell Sorting (FACS)provides a method for sorting specific cells from aheterogeneous mixture. Many objects are too largeor too sensitive for conventional FACS-based flowcytometry. The BioSorter-Pro (Union Biometrica®)has been developed for flow cytometry of objects ina wide range of sizes (10 to 1500 μm) with highspeed and high sensitivity.

Biosorter applications: counting, sorting,dispensing and analyzing- Combinatorial peptide libraries coupled to carrier

beads- Large cells, cell clusters or tissues- Embryoid bodies and cultured organoids- Zebrafish embryos- Plant seeds (e.g. Arabidopsis) - Microcolonies of Aspergillus and filamentous

bacteria

Access and pricingThe LPC facility was established with financialsupport from the Netherlands Organization forScientific Research (NWO Investment Grant) andUtrecht University (Support Core Facilities, LifeSciences). Fees for Large-Particle Flow Cytometry withoperator support are 50 € per hour for external usersfrom academia, and 100 € per hour for users fromindustry, determined in part based on total user timeand amount of support needed.

Your partner in counting, sorting,collecting, dispensing and analyzing

objects in the 10-1500 μm size range

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CELL SCREENING CORE

ServicesThe Cell Screening Core (CSC) is a well-establishedcenter for high throughput screening. It specializesin the development and use of advanced highcontent, cell-based assays for a wide variety ofapplications. Amongst these are projects for drugtarget discovery, and drug discovery.The CSC can screen libraries of chemicals, siRNAs,miRNAs and other reagents. The center has therequired laboratory facilities, robotics,bioinformatics infrastructure and expertise tosuccessfully run fully automated high throughputchemical biology and functional genomics screensin 96- or 384-well plates. The Core offers a highlypersonalized, collaborative service in which we workclosely with our clients to help them to achieve theirgoals, with high levels of quality and affordablecosts. The CSC has extensive experience inautomated screening in academia and thepharmaceuticals industry and can therefore offerclients access to a combination of industrial

experience with the support of academicknowledge and innovation.

Equipment- Caliper Sciclone Liquid Handling Robot- Caliper Twister II Robotic Arm x 2- Liconic Automated TC Incubator- MDC SpectraMax M5e Plate Reader- MDC AquaMax Plate Washer- Thermo Multidrop Combi Plate Dispenser - Thermo ArrayScan VTi Automated Microscope- Thermo Cellomics Image Analysis Bioapplications- Thermo Cellomics Store Image Database- Yokogawa CV7000 Automated Microscope- Agilent PlateLoc Plate Sealer- Agilent VCode Barcode Labeler- PE Columbus Image Analysis & Database

AccessThe CSC is open to all scientists from academic and commercial entities on the basis of a partialcost-recovery collaboration or full-cost servicedepending on the nature of the project.

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SINGLE CELL ANALYSISCENTER UTRECHT

The cell is a fundamental unit in Life Sciences, buttraditionally, many molecular and genetic assaysare based on material derived from thousands tomillions of cells, resulting in “average” values.However, no such thing as an average cell exists.Hence, genetic and transcriptome analysis of singlecells offers researchers a much deeperunderstanding of cell and tissue behavior, but istechnically challenging. We offer state-of-the-artequipment and expertise to perform automatedmicrofluidic processing of individual cells intobarcoded libraries for mRNA sequencing or DNAsequencing. Furthermore we provide support withbioinformatic analysis of single-cell datasets.

Equipment- Fluidigm C1 Autoprep system for microfluidic

preparation of pre-amplified cDNA or gDNAlibraries.

- Fluidigm Access Array system for microfluidicamplification of targeted single cell DNA libraries.

- (more to follow soon)

AccessThe facility is available to any group, from theUtrecht Science Park or elsewhere. We offer eitherpartial cost-recovery based collaboration or full-costservice.

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We offer automated microfluidicprocessing of individual cells into

barcoded libraries for mRNAsequencing or DNA sequencing

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FACILITIES FOR TISSUE AND

ORGAN RESEARCH

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DUTCH MOLECULAR PATHOLOGY CENTRE

We facilitate advanced, qualified and standardizedmolecular and pathological analysis of experimentalanimals for research of academic and industrialparties. Our team consists of board-certifiedveterinary pathologists with graduate andpostgraduate training in animal genetics as well ascellular and molecular biology.

EquipmentThe DMPC has a state-of-the-art facility forprocessing, staining and imaging of tissues fromexperimental animals. We offer full necropsyservice including gross examination of tissues,appropriate tissue fixation, slide preparation, andhistological evaluation. Pathological findings will bedescribed, digitalized, quantified and interpreted bya comparative expert team composed of veterinaryand human pathologists as well as animal modelscientists to evaluate the significance of the

pathological findings as animal model for humandiseases. We offer transgenic reporter markeranalysis, such as LacZ and GFP for monitoring tissuecell-type specific expression. In addition we offerquantitative measurements for proliferation-,apoptosis-, differentiation-, inflammation-, fibrosisstatus utilizing immunostainings on animal tissues.

AccessThis facility is available to any other group either inthe form of partial cost-recovery basedcollaboration or full-cost service.

We facilitate advanced,qualified and standardized

molecular and pathologicalanalysis of experimentalanimals for research of

academic and industrial parties

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DIGITAL HUMAN PATHOLOGY

ServicesThe Pathology Department of the UMC Utrechtperforms diagnostic testing of body samples suchas tissues (histology) and cells (cytology). Also,autopsies are performed to medical practice todetermine and evaluate the cause of death. In orderto assist in the diagnosis additional examinationcan be done including:- Immune- and enzyme histochemistry to

demonstrate abnormalities of proteins- Molecular diagnostics to indicate abnormalities in

the DNA in order to make a diagnosis and/ordetermine the sensitivity to a treatment

- Electron Microscopic examinationThe Pathology Department is home to the DutchSurveillance Center for Prion Diseases.

InfrastructureAs first university hospital in the world, theDepartment is making the transition to fully digitalpathology diagnostics. This not only results in fasterdiagnosis, it also yields a better interpretation of theimages: measurements through the microscope areless accurate than with a computer program ondigital images. Furthermore, it enables a more closecollaboration with the Department of Radiology bycombining digital pathological images with digitalradiological images. This makes it easier to establisha link between imaging and tissue diagnosis, todetermine for example whether a tissue biopsy isrepresentative. This increases the quality ofdiagnosis and treatment, and thereby improvespatient safety.

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We aim at expediting emergingbiofabrication technologies in

areas of regenerative medicine,3D in vitro cell culture models

and therapeutic treatment

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UTRECHT BIOFABRICATION FACILITY

We aim at expediting emerging biofabricationtechnologies in areas of regenerative medicine, 3D in vitro cell culture models and therapeutictreatments.The Utrecht Biofabrication Facility is a leadingEuropean knowledge center in the area ofbiofabrication, bringing together engineers,materials scientists, cell biologists, clinicians andcommercial partners to create a fosteringenvironment for development, evaluation andclinical translation of 3D tissue constructs. Theopportunities range from small prototyping jobs toco-development of large research projects,facilitated by the presence of key technologies andexpertise (researchers and technical staff).

Activities- Prototyping (CAD and MRI/CT-based anatomical

models)- Scaffold fabrication- Cell printing- Biomaterial/cell array preparation (robotic

dispensing)- Biomaterial/bio-ink development- 3D tissue construct development

Equipment- RegenHU 3D Discovery integrated bioprinter

setup- SysEng BioScaffolder bioprinter- Envisiontec Perfactory P3 Mini Multilens

stereolithography apparatus- Ultimakers

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FACILITIES FOR FUNGAL,BACTERIAL, ANIMAL AND

HUMAN RESEARCH

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LABORATORY FOR MEDICAL MICROBIOLOGY

The Department of Medical Microbiology is responsible for the diagnosis of infectiousdiseases (bacteriology, virology, serology, parasitology and mycology) and infectionprevention at UMC Utrecht.

ServicesIn addition, all kinds of trials are done in which microbiological diagnostics need to beperformed, both viral and bacteriological. We have the facilities to grow bacteriological andviral strains and perform molecular analyses. We have specific expertise in the field ofantimicrobial resistance and perform both molecular and phenotypic assays to determineresistance to bacteria as well as viruses. We have several clinical strains of viruses andbacteria for research purposes. We deploy next generation sequencing techniques in bacteriology and virology and are inthe final stage of setting up a pipeline for microbiome research.

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CBS-KNAW FUNGAL BIODIVERSITY CENTRE

CollectionThe CBS-KNAW Fungal Biodiversity Centre hosts aworld renowned collection of about 100,000 livingfungi, yeasts and bacteria. In its diversity of speciesit is unrivalled as a reference center for mycologicalresearch. Fungi may cause diseases of plants,animals and people. The center conducts innovativemycological research, examines fungal propertiesand develops novel fungal applications andsolutions to societal challenges based on the fungi’sunique genetic resources. The center’s largecollection and metadata are used intensively bypublic and private parties.

Services- Preservation of (proprietary) fungal collections.- Delivery of fungi, bacteria and plasmids from the

open collection.

- Identification and isolation of fungi, yeasts andbacteria.

- Detection of mycotoxins in food ingredients andfood products to ensure food safety.

- Analyzing fungal problems in indoor andindustrial environments including museums andarchives.

- Customized research & development and trouble-shooting projects for individual companies.

- Identification service for moulds, yeasts andbacteria and advise on control measures.

We host a collection of100,000 living fungi, yeasts

and bacteria

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We stimulate the development, acceptation andimplementation of methods which can replace,reduce and refine animal experiments, the 3R’s

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3Rs-CENTRE UTRECHTLIFE SCIENCES

The Centre facilitates 3Rs-research in many ways, byproviding information and advice on 3Rs-models. Itmaintains the 3Rs-Database Program that aims tofacilitate researchers implementing the 3Rs. Thisprogram makes state-of-the-art knowledge on the3Rs available for researchers in the pharmaceuticalor chemical industry and research institutions,students and others involved in animalexperiments. Currently, the program has adoptedthe Interspecies Database and the HumaneEndpoints website, which help to respectivelyreduce and refine animal experiments.

Interspecies databaseThe Interspecies Database provides insight intophysiological, anatomical and biochemicalparameters of different animal species andhumans. By using the database, researchers cansmarter design the animal experiments in terms ofchoosing the best animal model in the translation

of the data towards another animal species ortowards humans. This could lead to a betterresearch quality and a reduction in the number ofexperimental animals. Some users have indicated areduction of their animal use by 40%, with anaverage of 20% by consulting the database. TheInterspecies Database is used by, on average, 5500unique visitors per month from all over the world.All available data originates from peer-reviewedliterature.

Human Endpoints databaseThe Humane Endpoints website gives informationon humane endpoints and teaches to recognizehumane endpoints in rodents. Interventions canthen be made at the ‘right’ moment by terminatingthe experiment. The website contributes torefinement: the prevention of unnecessarysuffering in laboratory animals. The information onthe website is offered in the form of texts,photographs, videos and training modules. Thewebsite is available in different languages and usedby, on average, 5000 unique visitors per monthfrom all over the world.

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Your partner for imaging studies in animalmodels of human (brain) disorders

BIOMEDICAL MR IMAGING & SPECTROSCOPY FACILITY

Services- Elucidation of pathophysiology, recovery

mechanisms and treatment effects in preclinicaldisease models with MRI and MRS;

- Validation and improvement of the diagnosticpotential of clinical MR tools in experimentalmodels;

- Development and optimization of MR acquisitionand analysis techniques for biomedicalapplication.

The inherent characteristics of MR allow us toperform non-invasive, three-dimensional andmultiparametric biomedical research, in which wecan assess tissue status (structural MRI, diffusiontensor imaging), hemodynamics (perfusion MRI),functional activation (functional MRI, includingresting-state fMRI and pharmacological MRI) and

metabolism (MRS). When combined with contrastagents, MRI allows the detection of cells andmolecular entities (cellular and molecular MRI).These MR tools are combined with othermethodologies to correlate MR results withbehavioral, electrophysiological and histologicalmeasures.

EquipmentOur laboratory is equipped with two state-of-the-artanimal MR systems at 4.7 T (40-cm horizontal bore)and 9.4 T (20-cm horizontal bore). We have a HD CCDcamera for in vivo optical imaging and equipment fortranscranial magnetic stimulation in (small) animals.Our facility is accessible to external users and staff isavailable for (assistance with) MR data acquisitionand analysis, and biotechnical support.

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We perform non-invasive, three-dimensional and

multiparametric biomedicalresearch

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HYBRID OPERATIONROOM FOR LARGE ANIMALS

ServicesThe hybrid operation room for large animalsexperimentation provides state-of-the-art X-rayequipment in a clinical grade setting for preclinicalresearch. It is located within the university’s generalanimal facility and operated by highly experiencedresearchers and biotechnical support.The hybrid operation room is dedicated to latestage preclinical research in drug and medicaldevice development, facilitating the translationtowards clinical implementation. Due to the clinical-grade equipment and set-up, the facility alsoprovides the perfect place for productdemonstration and clinician training for first-in-mantrials and device handling.

InfrastructureThe hybrid operation room meets all your clinicalneeds. It is equipped with a Philips Allura FD20 C-arm with 3D reconstruction capabilities, powerinjection, 3D-ultrasonography, OCT and ECGmonitoring. The control room has direct view on theOR and is equipped with several displays includinglive camera view on the operation table that allowsfollowing and controlling the procedure from thecontrol room.

Your partner for clinical-gradepreclinical research

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FACILITIES FORCLINICAL RESEARCH

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FACILITIES DEPARTMENTOF FARM ANIMAL HEALTH

The Department of Farm Animal Health of the Facultyof Veterinary Medicine has state of the art facilitiesand comprehensive expertise on farm animal health,welfare, infectious diseases, metabolism, fertility andfood at its disposal. The animal facilities are modernand highly equipped to meet the needs of theanimals, researchers, students and teaching staff. In addition, several laboratories are available on thecampus of the Faculty of Veterinary Medicine formicrobiology (VMDC), molecular pathology (DMPC)and diagnostics (UVDL). The in vitro fertilization (IVF)laboratory offers a wide range of opportunities toinvestigate reproductive disorders and the impact of(metabolic) conditions in human and animals by theuse of oocytes derived from slaughterhouse ovaries.The combination of specialists on the different areasof Veterinary Medicine and in house biologists andepidemiologists creates an ideal scientificenvironment.

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JULIUS SUPPORT FORRESEARCH & TRIALS

ServicesJulius Support for Research & Trials has been set upto assist researchers with the design, conduct, datamanagement, analysis and reporting of medicalresearch. This ranges from drug research, todiagnostic and prognostic test and biomarkerresearch, to etiologic, bioinformatics, decisionsupport, and healthcare innovation research.

Our support includes primary studies and meta-analytical studies, conducted in primary-,secondary- or tertiary care or in the public healthsetting. The support can be called for during thestudy conception, grant application or innovationphase, during the phase of protocol writing, datacollection or data analysis, or during the reportingand dissemination phase.

We provide this support for all types of clinicalresearch, in all care settings, including:- Clinical trials- Research on causes of diseases- Research on the clinical value of (for example)

diagnostic and prognostic tests, predictionmodels and decision support tools, E-health andself-monitoring tools, medical devices andtechnology, and healthcare innovations

- Systematic reviews and meta-analysis- Access to primary care networks with more than

250,000 patients and various other cohorts- Access to over 1,000 clinical sites globally- Scientific support to grant proposals (e.g. for

the EU)

State-of-the-art expertise andknowledge of all necessary clinical

research disciplines

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UPOD – UTRECHT PATIENT ORIENTED DATABASE

DatabaseDiagnostic algorithms and clinical research dependon proper identification and characterization ofpatient populations. The UPOD database is aunique relational database collecting andcombining clinical characteristics, medication andlaboratory measurements since 2000 andencompasses data from more than 400,000individuals and >90,000,000 laboratory results(including pediatric patients). The UPOD is operatedby the Laboratory of Clinical Chemistry andHematology in close collaboration with thePharmacology department.

ServicesRequests for data are handled by a multidisciplinaryteam and reviewed to comply with institutionalethical standards. The multidisciplinary team alsoprovides competent expert advice on data analysis

including epidemiology support and clinicalchemistry and pharmacology expertise. UPOD also includes one-of-a-kind background dataon blood cell characteristics that are measuredduring routine clinical hematology analysis. Thesedata comprise >70 additional parameters notreported in clinical routine and have been shown toprovide highly predictive outcome markers invarious diseases. Raw hematology analyzer files arealso available upon request from >2,000,000measurements for further in-depth analysis.

ParamatersSelection of parameters included in UPOD: Clinicalchemistry, Hematology, Medication, ICD codes,Radiology, Pathology, Procedures, Diagnosis, Patientcharacteristics, Length of stay, FCS files, ICU, NICU,PICU, Cohorts, Transfusion.

Your partner for combiningclinical diagnostics and research

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UTRECHT BIOINFORMATICS CENTER

The Utrecht Bioinformatics Center (UBC) bringstogether the bioinformatics expertise on theUtrecht campus into one easy to access community.Utrecht houses bioinformatics expertise at a world-class level for widely different biological and datadomains. We aim to combine this expertise, createnovel expertise and new bioinformatics approachesto perform world-class integrative bioinformaticsand to enable scientific breakthroughs in a widevariety of relevant life sciences. Specifically, we aim

to unify the separate bioinformatics teams into theUtrecht Bioinformatics Center (UBC) to create anintegrative bioinformatics community at the UtrechtScience Park to strengthen our future research.

FacilityThe UBC provides access to bioinformaticians andtheir expertise, a campus-wide High PerformanceCompute (HPC) facility for Life Science research aswell as training and education in severalbioinformatics (related) topics.

AccessThe center and the facility is available to life sciencescientists and students.

The Utrecht Bioinformatics Center (UBC) bringstogether the bioinformatics expertise on the Utrecht

campus into one easy to access community

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PHARMACY LABORATORY

ServicesThe Pharmacy Laboratory of the UMC Utrechtperforms analyses for clinical pharmacology andtoxicology for patient care and clinical research. Inaddition, quality control is performed on (in-house)pharmaceutical preparations including shelf lifetests and formulation tests as well as quality controlof the raw materials. There is a whole range ofroutine analyses that take place at least once a year.Most methods are based on LC-MS/MS technology.

ResearchThe central research theme of the PharmacyLaboratory is to explain, predict and improve thevariability of patient response to pharmacologicaltreatment through clinical pharmacology andclinical pharmaco-epidemiological approaches.

This theme is inspired from the problems in patientcare. There are three lines of research:- Drug innovation in pediatrics - Central nervous system pharmaco-epidemiology - Biomarkers for drug response

EquipmentThe following techniques are used for analysis ofpatient material: immunoassay, AAS, HPLC/UV-fluorescence, GC, LC-MS/MS, UV-spectrophoto-metry. For quality control of raw materials andpreparations additional techniques are usedincluding IR, titrimetry, TLC, osmolarity andchemical tests. The Pharmacy Laboratory at UMC Utrecht is CCKLaccredited.

We run more than 150 routine lab tests in a CCKL-accredited

environment

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MULTIPLEX CORE FACILITY

ServicesThe MultiPlex Core Facility of the UMC-Utrecht isstate-of-the-art and offers multiple immune-assaysbased on color-coded microspheres (xMAPtechnology), which is used for “tailor made” immunemonitoring in cohort studies. The MultiPlex CoreFacility is ISO9001:2008 accredited and measuresover 160 immune related mediators (cytokines,chemokines, adipokines, growth factors and matrixproteins) using less than 100 μl sample volume.Protocols are optimized for culture supernatant,plasma, serum, synovial fluid, liquor, ocular fluid,lysed tissue, urine, dried bloodspots and middle eareffusions. The assays are sensitive, precise,optimized and validated and are setup in a flexibleand open-architecture infrastructure which allowsrapid measurements of any customizedcombination.

EquipmentThe MultiPlex Core Facility operates two FlexMAP3Dsystems in combination with various 96 and 384well automated washers. Furthermore variousstatistical software packages are in place foranalyzing and handing ‘big data’.

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Your partner for tailor-madeimmune monitoring

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UTRECHT CENTER FORDIAGNOSTIC ADVANCES IN IMMUNOLOGY RESEARCH

The Utrecht center for Diagnostic Advances inImmunology Research (U-DAIR) provides an extensiveImmune Monitoring platform to study immunologicalparameters in patients with immune-mediateddiseases and to assess the effects of immunemodulatory treatment. Harmonized and standardizedapplication of diagnostic and state-of-the-art researchmethodologies enable validated biomarker researchand comparison of biomarker profiles between patientcohorts. Upon request we develop, optimize andvalidate analytical tests to facilitate research ondisease-specific parameters.

Core Competences- High throughput quantification of (adipo) cytokines,

chemokines and growth factors in multiplex assays(160+ analytes in 250 uL biological fluid).

- Extensive phenotyping of leukocytes (e.g. T, B andNK(T) cell lymphocyte subsets) using flow cytometry.

- Functional assays on cytotoxicity and assessment ofintracellular cytokine profiles in (un)stimulatedlymphocytes.

- Assessment of allergenicity and patient allergicphenotypes by Basophil ActivationTest (BAT).

- HLA-typing, SNP analyses, TCR- and IG-sequencingusing next generation sequencing.

- Pharmacological screening for immunologicalparameters and PK-PD modeling.

- Development and validation of novel immunoassays,including immunoassays for biologicals.

- Access to generic diagnostic services forimmunological parameters in Autoimmune diseases,Allergy, Transplant Immunology, Immunedeficiencies, Oncology, Hematological malignancies,and Acute & Chronic Inflammation.

- Access to certified laboratory analysis: ISO9001, EFI,CCKL.

U-DAIR is positioned within the diagnostic departmentof the Laboratory of Translational Immunology (LTI)and aims to accelerate and improve immunologyresearch and diagnostics.

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HIGH-THROUGHPUTISO-CERTIFIED ELISA FACILITY

Biomarker research requires reliable analyses oflarge sample numbers in a high-throughputfashion. The ISO-certified ELISA facility is embeddedin the Department of Clinical Chemistry andHematology of the UMC Utrecht and is operated inclose collaboration with clinical chemistry experts.This ensures high-quality measurements of pre-clinical markers that can be combined withmeasuring clinical standard laboratory parametersin the routine lab.

ServicesThe ELISA facility has been set up to enable high-throughput measurements of a variety ofparameters. Additional analytes can be measuredor set up upon request. Prices depend on analytesand batch size but usually range between 3 and 4 Euro per sample including sample handling.

InfrastructureThe ELISA facility is specifically suited to handlebatch measurements. Samples are usuallymeasured in a 384 well format. Using this format,about 2000 samples can be handled and measuredper day.

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Your partner forbiomarker research

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FACILITIESFOR

TECHNICALSERVICES

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MEDICAL DEVICE PROTOTYPING FACILITY

Our facility under ISO 13485 certificated offersMedTech startups and external researchers thepossibility to translate their concepts into functionaldemonstrators and even prototypes for clinical use.

Equipment and servicesThe infrastructure includes several traditionalmilling and turning machines as well as ComputerNumerical Control (CNC) machines. Also ComputerAided Manufacturing (CAM) software to translateComputer Aided Designs (CAD) into CNC codes isavailable and offered as a service. Other servicesinclude for example laser cutting and marking ofmaterials, cutting and bending of materials. Ourfacilities and dedicated instrument makers operateunder an EN ISO 13485:2012 and are certificated toensure safe product manufacturing and assembly,suitable for clinical investigation in our hospital andpartnering institutes.

Besides our own equipment we have a strongnetwork of companies specialized in dedicatedfabrication techniques, such as for example 3Dprinting, water jet cutting.

AccessWe offer this facility service to researchers andhealthcare workers of the UMC Utrecht, and also toMedTech companies. Together with Pontes Medicalwe assist (startup) companies to help them buildtheir demonstrator for preclinical investigation orbuild their medical device prototype for clinicalevaluation in the UMC Utrecht and/or with alliedpartners.

Bringing medical devicedevelopments to the clinical

practice in a safe andefficient manner

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SCIENTIFIC INSTRUMENTATION

Do you have a technical problem in your researchor education project? Scientific Instrumentation canhelp. Simply contact us using the informationbelow. Our specialties:

Engineering Group Engineering develops new equipment, electronics,instruments and tools. We give advice and realisedetailed designs in cooperation with our clients.Areas of expertise: 3D printing, fine mechanics,optics, (UHV) vacuum, thermodynamics,electromechanics, micro-controller design, dataacquisition (incl. for CERN), interfaces,programmable software and hardware (VHDL).Production, Service and Maintenance Production, Service and Maintenance processesmetal and plastics and also provides a full-range ofmaintenance services to all laboratory equipment.

Our machine park is capable of CNC milling andmachining up to five axes simultaneously,conventional machining and wire- and sparkmachining.

Glass workshop The Glass Workshop makes all laboratoryglassware. We use cold and warm processing onvarious types of glass and ceramics. Cold glass ismilled or machined ultrasonically using a DMG-DM-US50. We also have the equipment needed to saw,hone, strip, glue, grind, polish (flat and round),sinter at high temperature and vacuum-solder.

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No job too small or too complex!

Utrecht Science Park | info@utrechtsciencepark.nl | T +31 (0)30 800 44 99 | www.utrechtsciencepark.nl

If you are interested in any of the referenced facilities or services please contact researchfacilities@utrechtsciencepark.nl.For further information visit our website at www.utrechtsciencepark.nl.

THIS BOOKLET IS PREPARED WITH SUPPORT OF:

AND WITH CONTRIBUTIONS FROM:

THE BOOSTER INITIATIVE IS SUPPORTED BY THE ADVISORY BOARD MEMBERS:- GERRIT VAN MEER, DEAN FACULTY OF SCIENCES, UTRECHT UNIVERSITY- ERIK HACK, CHAIRMAN INFECTION & IMMUNITY, UNIVERSITY MEDICAL CENTER UTRECHT- ANDRÉ HENKEN, DIRECTOR FACULTY OF SCIENCE AND TECHNOLOGY, UNIVERSITY OF APPLIED SCIENCES

CREDITSUTRECHT SCIENCE PARK ©2015DESIGN: WAT ONTWERPERSPHOTOGRAPHY: UTRECHT UNIVERSITY, UMC UTRECHT, HOGESCHOOL UTRECHT, SHUTTERSTOCK.COM