Accelerate Your ResearchQuality biospecimens,

unique to your project.

Primary Human Biospecimens

Diseased and Healthy Whole Blood

Processed Blood Derivatives

Dissociated Tumor Cells (DTCs)

Formalin-Fixed Parrafin Embedded Tissue Blocks (FFPE)

Flash Frozen Tissue Blocks

Matched Blood and Tissue

Your Quality Source For:

"Conversant provides premium quality primary human samples along with detailed information on patients in a timely fashion. We routinely observe >85% cellular viability from the thawed primary samples obtained from both cancer and non-cancer patients."

9001 Certified

-Obdulio Piloto, PhD, Research Scientist, Exelixis, Inc.

Blood Products

CheckPoint Inhibitor/Immunotherapies

Mutation Characterized Blood

Healthy Donors/Normal Controls

Opdivo, KeyTruda, Yervoy, and Tecentriq Treated Patients

PD-1, PD-L1, CTLA4 Inhibitor

Circulating Biomarkers for Immunotherapy

Longitudinal Collections

Outcome Data/Responders vs. Non-Responders

Longitudinal Collections 

Known HLA. Confirmed HLA-A02. *Tested via NGS sequencing with 6 digits on the allele

Growing and Diverse Donor Pool (Age/Race/Gender/Time Matched)

Virus Tested

Custom Processing and Cell Isolations

At Conversant Bio, we offer a wide variety of human peripheral blood samples

from a number of different indications, including normal patient populations.

We also provide patient annotation for our blood samples including age, race,

gender, smoking history, alcohol history, primary diagnosis, treatment history,

known mutation statuses, and more.

Early and Late Stage Solid Tumors

Non-Small Cell Lung Cancer

Colorectal Cancer

Prostate Cancer

Ovarian Cancer

Breast Cancer

Melanoma

Pancreatic

More

Clinically Observed Mutations (Known Variants)

EGFR

KRAS

ALK

MET

ERBB2 (HER2)

RET

HRAS

TP53

BRAF

NRAS

Known Wild Types

More

Double-Spun Plasma

Processing Let our experienced team handle all of the complex processing, so you can

focus on the important things. Our standardized double-spin protocol has been

optimized to provide the highest yields of cell-free DNA with low gDNA

contamination. We preserve both the buffy coat and clarified plasma for analysis

of unique somatic mutations.

Cell-Free Nucleic Acid Protocol Whole blood collected in Conversant Bio provided Streck Cell-Free DNA BCT®.

Initial spin at 1,500 x g for 10 minutes to separate blood fractions.

Carefully aliquot plasma fraction into nuclease-free tube. Save buffy coat.

Spin isolated plasma fraction at 16,000 x g for 10 minutes to yield a

supernatant plasma free of excess cellular debris and gDNA contamination.

"To remove possible gDNA contamination from white blood cells, we need the

blood samples to be collected in Streck and spun twice and the speed for the

second spin is 16,000 x g for 10 mins. Our analysis would be very sensitive to the

contamination."

About Streck Cell-Free DNA BCT® Cell-Free DNA BCT® is a blood collection tube with a formaldehyde-free

preservative stabilizing nucleated blood cells. This unique stabilization prevents

the release of genomic DNA, allowing isolation of high-quality cell-free DNA

which can be further used for a wide range of downstream applications in

clinical research studies, drug discovery and diagnostic assay development.

Cell-Free DNA BCT® reduces the need for immediate plasma preparation due to

its unique stabilization properties. Cell-Free DNA is stable for up to 14 days,

while circulating tumor cells are stable for up to 4 days, at room temperature,

allowing convenient sample collection, transport and storage.*

Nucleic Acid Extractions

Platelet-Rich Fractions (Tumor-Educated Platelets)

Circulating Tumor Cells

Exosomes

Cell Isolations

Customized protocols available as well:

-Senior Scientist, Major U.S. Diagnostic

Cell-Free DNA BCT® is registered copyright of Streck® * Claims made by Streck® https://www.streck.com/product.aspx?p=Cell-Free%20DNA%20BCT

Dissociated Tumor Cells

How are these tumors processed? - Dissociated Tumor Cells are obtained by processing resected

tumor tissue using both enzymatic and mechanical techniques. The goal is to preserve cell

surface markers while maintaining viability and cell counts for the diverse cell population

within each tumor. Most of our R&D in 2015 was geared towards customizing protocols (by

disease indication) to ensure maximum cell viability.  For this reason, we consider our exact

dissociation protocol methods to be proprietary.

Why use dissociated tumor cells? - Tumor tissues are a critical component of many oncology

research projects. However, fresh tissues are increasingly harder to identify, accrue, ship,

process, and store. Conversant Bio offers Dissociated Tumor Cells to researchers looking for a

source of high-quality primary cells, without the hassle of fresh tissue accrual.

We currently have 2,000+ aliquots in stock from over 200 unique resections.  Each aliquot has

over 1 million cells and >50% cell viability.  

20

40

60

80

Colorectal

Ovarian

Endometrial

Kidney

Melanoma

Lung

0

Unique Dissociated Tumor Cell Patients Available

“We are clearly able to detect viable myeloid populations in the RCC DTCs. There appears to be

fairly similar distributions across the three samples which is great. We’re showing T cell, B cell,

NK, Monocyte and Neutrophil percentages of CD45+ Viable cells.“

-Researcher, Clinical-Stage Therapeutics

Surgical Tissue

All blocks were from US-sourced, patient cases confirmed by two U.S. Board Certified Pathologists. Six

5-micron unstained tissue slides per case were supplied. One slide was H&E stained and reviewed by

a CLIA Lab pathologist. The pathologist circled the most appropriate area of tumor tissue for testing

and estimated the tumor density within the circled region. Tissue from unstained slides was

microdissected using the H&E stained slide as a guide. DNA was then extracted from the dissected

tissue using one of two chemistries, depending on the quantity of tumor tissue available for testing.

Spectrophotometric analysis of the optical density (O.D.) at 260 and 280 nm was performed to

determine the quality and quantity of the extracted nucleic acids.

Surgical Tissue

Conversant Bio's FFPE tissue bank is designed to meet your quality needs. We have thousands of

pathologist reviewed archival tissue blocks from US surgical cases available in our biorepository.  

Our samples were specifically selected to aid in discovery, validation, and controls of both antibody

and molecular testing to help you deliver on your project. 

Formalin Fixed Paraffin Embedded Tissues

Mutation Testing Process

We offer ALK, cMET and MSI cases. Other mutations available include:

Mutation Characterized FFPE

"We found that using Conversant Bio-sourced FFPE blocks increased the

success rate of our research by 40%. Their quality saves us time and money."

-Biomarker Scientist, International Biotechnology Firm

Lung - EGFR and KRAS 

Breast - PIK3CA

Colorectal - KRAS, BRAF and NRAS

Melanoma - BRAF and NRAS

We have 1000 positive cases across all of these mutations, including

many that are wild type.

DTCs and PBMCs are matched pre-surgical blood and treatment naive tumor tissue. The tumor is

processed to single cell tumor suspension and blood is processed to mononuclear cells.

These biospecimens are ideal for researchers who are looking to study immune cells within the tumor

and identify ways to activate immune response to attack tumor cells. These sets allow for a comparison

of the immune population in the tumor and the immune population in the peripheral blood.

Matched Sets

FFPE and Double-Spun Plasma

The Process 1. Blood collected just before surgical resection in EDTA tubes.

2. Blood is double-spun within two hours of collection and plasma is

stored at negative 80 degrees Celsius.

3. Plasma is then matched with FFPE resected tumor tissue.

Melanoma, Renal Cell Carcinoma, Colon, Ovarian, and Lung Cancers

All Samples are Pre-Treatment

All Stages Represented

 Dissociated Tumor Cells and Peripheral Blood Mononuclear Cells

Patient Profiles Data ProvidedBase Demographic Data

Pathology Report

Current Treatment

Block QC Data

Contact Us Today sales@conversantbio.com

866-838-2798

Conversant Bio 601 Genome Way, Suite 1200

Huntsville, AL 35806 (866) 838-2798

www.conversantbio.com

Prostate Cancer

Breast Cancer

Non-Small Cell Lung Cancer

Small Cell Lung Cancer

Colorectal Cancer

Melanoma

Pancreatic Cancer

Head and Neck Cancer

Bladder Cancer

Kidney Cancer

Liver Cancer

Glioblastoma

Ovarian Cancer

Gastric Cancer

CML/CLL

Oncology Autoimmune

Hematology

Crohn’s 

Ulcerative Colitis

Systemic Lupus Nephritis

(SLE)

Rheumatoid Arthritis

Sjogren's

Psoriasis/Psoriatic Arthritis

AML/ALL

Multiple Myeloma

MGUS/MDS

Hodgkin’s and NHL

Lymphomas

Healthy Donors Age/Race/Gender

Matched Controls

Known HLA 

Virus Tested

OtherPulmonology

Neurology

Cord Blood

More

Primary Sample Indications