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![Page 1: 1000 1200 1400 1600 1800 2000 s d i Web Interface s o ... · a plant integrative ‘omics’ database Gabi Primary Database Species represented in GabiPD Gene-centric view Barley](https://reader033.vdocuments.us/reader033/viewer/2022043007/5f95a73cc971597abd00e211/html5/thumbnails/1.jpg)
a plant integrative ‘omics’ database
Gabi Primary Database http://www.gabipd.org/
Species represented in GabiPD
Gene-centric view
Barley and Solanaceae genetic maps
Source for figures:http://www.gabi.de/http://en.wikipedia.org/wiki/DNA_microarrayhttp://en.wikipedia.org/wiki/Image:2D_gel_images_dual_channel_warped.PNG
Diego Mauricio Riaño-Pachón, Axel Nagel, Robert Wagner, Rico Basekow, Jost Neigenfind, Elke Weber, Sabrina Kleessen, Birgit KerstenGabiPD team, Bioinformatics group, Max Planck Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Potsdam - Golm, Germany
Max Planck Instituteof Molecular Plant Physiology
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GabiPD
ReferencesAPG. 2003. An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG II. Bot J Lin Soc. 141:399-436Ball C.A. and Brazma A. 2006. MGED standards: work in progress. Omics. 10:138-144.Bohs L. 2005. Major clades in Solanum based on ndhF sequence data. pp 27-49 in Keating RC, Hollowell VC, and Croat TB (eds.), A festschrift for William G. D'Arcy: the legacy of a taxonomist. Monographs in Systematic Botany from the Missouri Botanical Garden, Vol. 104. Missouri Botanical Garden Press, St. Louis, MO.Bonfield J.K. and Beal K.F., et al. 2002. Trev: a DNA trace editor and viewer. Bioinformatics. 18:194-195.Jenkins H., Hardy N., Beckmann M., et al. 2004. A proposed framework for the description of plant metabolomics experiments and their results. Nat Biotechnol. 22:1601-1606.Knapp S . 2002. Tobacco to tomatoes: a phylogenetic perspective on fruit diversity in the Solanaceae. J Exp Bot. 53:2001-22.Sol Genomics Network (http://www.sgn.cornell.edu/about/about_solanaceae.pl)Soltis PS and Soltis DE. 2004. The origin and diversification of angiosperms. Am J Bot. 91:1614.Stein N., Prasad M., Scholz U., et al. 2007. A 1,000-loci transcript map of the barley genome: new anchoring points for integrative grass genomics. Theor Appl Genet. 114:823-839.The Tree of Life (http://www.tolweb.org/angiosperms)Usadel, B., Nagel, A., Thimm, O., et al. 2005. Extension of the visualization tool MapMan to allow statistical analysis of arrays, display of corresponding genes, and comparison with known responses. Plant Physiol. 138:1195-1204.
http://commons.wikimedia.org/wiki/Image:Gouache-arabidopsis-thaliana.jpghttp://commons.wikimedia.org/wiki/Image:Arabidopsis_thaliana-flower.jpghttp://drnelson.utmem.edu/module5/BSA/Spectrum/BSA60ng%20.jpg
GabiPD application structure
GabiPD
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Genome,Transcriptome Metabolome Proteome
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DataPerl API
Java API
GreenCards
BioTools
YAMB
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BioMoby
Web Interface
Web Services
Templates
API Generator
Schema
Data Insertion &Update Routines
Database
meta information and api generation
data flow and data processing
generate
read
read
Populus trichocarpa
Populus tremula
Medicago truncatula
Arabidopsis thaliana
Brassica napus
Vitis vinifera
Solanum tuberosum
Solanum lycopersicum
Capsicum annuum
Nicotiana tabacum
Beta vulgaris
Hordeum vulgare
Oryza sativa
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gio
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erm
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Salicaceae
Salicaceae
Fabaceae
Brassicaceae
Brassicaceae
Vitaceae
Solanaceae
Solanaceae
Solanaceae
Solanaceae
Amaranthaceae
Poaceae
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Poaceae
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Schematic overview of the GabiPD application structure. The ´API generator´ translates the ´Templates´ using the database meta-information (shown in blue), generating the database application interface (Java and Perl API). The application logic (shown in yellow), i.e., Web Interface, WebServices and data manipulation routines, interacts with the ´Database´ through the database application interface.
‘omics’ data2.
Phylogenetic tree depicting the evolutionary relationships among species represented in GabiPD. Species for which whole-genome sequences and annotations are available are shown in blue. *Species that will soon be integrated in GabiPD. Species not shown: Solanum bulbocastanum, S. demissum, S. phureja, S. spegazzinii.
Example of a keyword search using GreenCards. (A) The user had performed a search for the keywords 'FLOWERING LOCUS T', which retrieves links to the GreenCards of Genes (genome annotation projects), Clones (ESTs) and Plants (mutant plant lines). (B) Display of the Gene GreenCard, corresponding to the Arabidopsis annotated gene AT1G65480.1. Here the users find high confidence matching EST sequences displayed in the 'Related with' section. Sequence features and the sequences themselves are displayed as well. The selected gene has a matching EST (Clone: MPMGp2011E01215), this Clone GreenCard is shown in (C) and links back to the Gene GreenCard and to the original EST trace file, displayed by JTrev (D). A protein spot in rapeseed (Brassica napus) has been identified by 2-DE/MS related to the retrieved gene, and a link directs the user to the 2DE-gel with the identified protein spot highlighted with blue cross-hair (E). The spot identified links to a description of the protein (F) that provides links to the original Gene GreenCard.
FunctionalannotationFunctionalannotation
ExternalresourcesExternal
resources
Sequencefeatures
Sequencefeatures
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Visualisation of expression profile data in MapManWeb. (A) The Affymetrix®NASC Array experiment on programmed cell death in Arabidopsis is displayed(NASCArrays reference number: 30). MapManWeb allows the visualisation of expressedgenes in different biological processes; here only probesets (i.e., genes) involved intranscription regulation are shown. (B) Details for a strongly down-regulated probeset,with links to the related Gene GreenCard in A. thaliana. (C) The Gene GreenCard for theselected gene (ANAC79) links back to the probeset of the Affymetrix® ATH1 array.
Visualising expression data5.
Visualisation of the genetic maps published by Stein et al. 2007. (A) Theregion between 0cM and 25cM of barley chromosome I is shown. A selected marker isdisplayed in red, and links to the Marker GreenCard (B), which contains information on arelated EST sequence therewith connecting genomic with transcriptomic information.With the EST description, cluster information is included (Contig30438.1) that links to theschematic representation of all ClusterContig members displayed onto the relatedconsensus sequence (C). EST sequences that were selected from this ClusterContig asrepresentatives for the new 27K barley unigene set are shown in turquoise.
B
CA
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Relatedclones
Relatedclones
Identifiedin 2DE gelsIdentified
in 2DE gels
GabiPD allows a seamless integration of varied ‘omics’ data types obtained from plant systems.Gene expression and metabolic profiling data, are included in GabiPD and integrated in gene-context.
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Link to providerLink to provider
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This work was supported by the German Ministry for Education and Research (BMBF) [GABI I: 0312272, GABI II: 0313112 and GABI-FUTURE: 0315046], the former German Resource Center for Genome Research (RZPD) GmbH and the Max Planck Society.
Funding
Genomanalyse
im biologischenSystem PflanzeGabiPD NAR paper doi:10.1093/nar/gkn611GabiPD NAR paper doi:10.1093/nar/gkn611