zooplankton analysis of water sample of fish seed

www.wjpps.com Vol 8, Issue 8, 2019.

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Sirisha et al. World Journal of Pharmacy and Pharmaceutical Sciences

ZOOPLANKTON ANALYSIS OF WATER SAMPLE OF FISH SEED,

STOCKING TANK

D. Sirisha* and Tasneem Jahana*

Centre for Environment and Climate Change (CECC) School of Environment Sciences

(SES) Jawaharlal Nehru Institute of Advanced Studies, (JNIAS), Hyderabad.

ABSTRACT

The high availability of the wild fish seed in the back waters of

Krishna and Godavari district is promoting the aquaculture in most of

the coastal districts of Andhra Pradesh. Seed collection operations are

practiced by the fisherman in theses villages. Large scale seed

collection is practiced in these regions. These collected seeds are

stocked in small ponds near the collection sites. The ponds water for

seed rearing is analysed by paper microscope and results are presented

in the paper. The photographs indicated the presence of Euglena,

paramecium and cladocera.

INTRODUCTION

Shrimp fish Culture using locally available wild seeds is gaining

importance in Andhra Pradesh. Due to the availability of sufficient

quantity of wild species of prawn fish in the backwaters of Krishna and Godavari district.

Aquaculture of those fishes has been established in most of the coasted district of Andhra

Pradesh. Two methods of seed collection operations are carried out in these villages.

Traditional methods follow the tidal pattern. In these villages the prawn seed are collected by

scoop nets dragnets using during low tide time from the water pools of mangroves

/backwaters of Krishna and Godavari. The seeds gets collected in the net during low tide time

and when water recedes further they are collected by the fisher’s man. The collected seeds are

stocked in small ponds near the seed collection sites. These seeds are stocked in Hapas or

releases directly to the pond of 1-2 are in size. The ponds for seed rearing are prepared with

two different depth the deeper depth in prefers and shallow depth in the middle. The stocked

fishers are allowed to grow for a certain period and they are sold to the fish farmers.

WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES

SJIF Impact Factor 7.421

Volume 8, Issue 8, 721-731 Research Article ISSN 2278 – 4357

*Corresponding Author

D. Sirisha

Centre for Environment and

Climate Change (CECC)

School of Environment

Sciences (SES) Jawaharlal

Nehru Institute of Advanced

Studies, (JNIAS),

Hyderabad.

Article Received on

23 May 2019,

Revised on 12 June 2019,

Accepted on 03 July 2019

DOI: 10.20959/wjpps20198-14248


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In the present study seed rearing pond waters are analysed by using Foldscope, invented[1-22]

by Dr. Manu Prakash which is based on Frugal innovation’s distributed by the Department of

DBT for conducting research studies. Foldscope is used for various studies including physics,

biology, Environmental Sciences and chemistry.[23-30]

Selection of Study Area

Two ponds in the Srungavruksham Village were selected. This village is in Palakoderu

Mandal of West Godavari District. It is located 64 kms east from District Head Quarters

Eluru.

It is located 64kms towards East from district headquarters Eluru, the latitude is 17.246991I

and latitude is 82.440441I. It is 11 meters above sea level. It is 402 kms from Telangana State

capital Hyderabad. The local language is Telugu. The total population of this village is 11080

the people of this village is 11080 they cultivate fish and shrimp. This area has many

hatcheries which supply fish prawn seedlings to entire region. Taking that factor into

consideration, present study has taken up this area.

Maps: (West Godavari)

Figure 1 - Study Area, West Godavari District, Andhra Pradesh.


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Palakoderu Mandal Map

Figure 2- Palakoderu Mandal, West Godavari District, Andhra Pradesh.

Srungavruksham Village

Figure 3 - Srungavruksham, Palakoderu Mandal, West Godavari District, A.P.


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Seed Collection Water Photographs


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METHODS AND MATERIALS

Qualitative Analysis

Zooplankton samples were collected from the ponds during the harvesting of fish. Plankton

net, made of bolting silk cloth, (mesh size approximately 0.6mm) was used for collecting the

samples. The bolting cloth was attached to a collecting bottle of 100ml capacity samples were

collected from the surface water at 1m depth from two place viz near shore and at the center

(open water) of the ponds. The samples are collected from benthic zone.

For each sample 50 liters of water was filtered through the net and transferred to the screw

capped bottle of 100 ml capacity. All the samples were preserved in the field by adding 4%

formalin Solution and brought to the laboratory for observation. The plankton was observed

under the Foldscope microscope and identified up to genera.


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The Photographs of the slides observed under Foldscope were shown in

FISH SAMPLE

LEGEND FOR FIGURE

The water sample shows different zooplankton like

EUGLENA

PARAMECIUM

CLADOCERA

Quantitative Analysis

The Preserved Sample were centrifuged and the Settled Plankton was used for Quantitative

study for Zooplankton count by using a Sedgwick Rafter counting cell.

Picture of Sedgwick Rafter Counting Cell.

Figure – 4.


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RESULTS AND DISCUSSIONS

The seedlings stock pond of fishes consists of Euglena, paramecium and cladocera. The

Presence of paramecium is high compared to Euglena and cladocera.

The collected water samples were analysed for its colour, odour, turbidity, dissolved oxygen,

pH and temperature. The results were given in Table I.

S.No. Characteristic

Property Observation Results

1 Colour Greenish –

Brown

Indicates the presence of Cyano bacteria

green algae

2 Odour Fishy Smell

3 Turbidity Slightly turbid has to be managed for reduction of turbidity

4 Temperature 220

c Suitable for growth of prawn

5 Dissolved oxygen 2.9 ppm has to be increased and modified

6 pH 6.8

7 Salinity 18 ppt

Microscopic Study

The water samples are examined under the microscope using 10X magnification and

observed different Zoo planktons and Larvae / eggs of shrimp / fishes. The slides made up of

water samples were studies by Foldscope and images were photographed. The species were

identified and labelled.

Table 2 (Fish Seed Stocking Tank, Zooplankton Distribution).

Quantitative Analysis of Zooplankton Fish Sample

S.No Name of Zooplankton Density of Zooplankton

1. Euglena 1050/Liter

2. Paramecium 2010/ Liter

3. Cladocera 940/ Liter

Total 4000/ Liter

CONCLUSION

Many aquaculture pond farmers are people are depending on experts or the fisheries

departments for maintenance of their ponds and for analysis of water analysis for

zooplankton / phytoplankton analysis. But the results are not trust worthy as a few members

are taking up this task, so the farmers must be analyse their samples with a simple kit which

is able to do themselves Billions of people in India are able can afford only in expensive

products. They should be affordable and there should be sustainable. Indians follows the

traditions of jugaad-Indian style of ingenuity. I can do attitude driven by necessity.


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The Following photographs represent the collected photographs - Innovation – More from

less or more (G.Santosh Kumar).

Innovations of Indians - Innovation - More from less or more - G.Santosh Kumar


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The innovation satisfied the Mahatma’s Tenant. I would prize every invention of science

made for the benefit for all.

Dr. Manu Prakash Foldscope invention benefited a common man, Scientist, a curious child in

every field of sciences. The first time users, pond hatcheries Labour were very enthusiastic to

use the Foldscope which has democratized the science from lab to the common man.

ACKNOWLEDGEMENT

The author is thankful for the support received from Department of Biotechnology (DBT),

Foldscope Instrument-Inc. and Prakash lab (Stanford) for funding the Project and providing


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Foldscope received under the program - use of Foldscope as a Research tool. The author is

thankful to Registrar, JNIAS and Department of Zoology, St. Ann’s College for Women.

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zooplankton analysis of water sample of fish seed

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