zetasizer nano series - dksh

ZETASIZER NANO SeriesPERfoRmAncE, SImPlIcITy, VERSATIlITy

www.malvern.com2

Performance, Simplicity, Versatility

Key benefits of the Nano Series

• Choiceoftechnologiesinacompactunitgivesexceptionalversatility

• Simplicityofoperationmeansminimaltrainingandrobustresults

• Highsensitivityfornanoparticles,proteinsandmacromolecules

• Disposablezetapotentialcuvetteforfast,accurateandeasymeasurements

• Highopticalqualityandtemperaturecontrolensuresaccuracyandrepeatability

• Novelmicrorheologyoptiontodetermineviscoelasticproperties

• MPT-2Autotitratoroptionforautomatedtrendmeasurements

There’s a Zetasizer Nano for every application

• Shortendevelopmenttimeforcolloidandemulsionformulations

• Improveformulationstability

• Assessproteinformulationstability

• Exploreproteinaggregationandoligomerizationstate

Technologies incorporated

• DualangleDynamicLightScattering(DLS)

• Non-InvasiveBack-Scatter(NIBS)

• StaticLightScattering(SLS)

• ElectrophoreticLightScattering(ELS)

• Mixedmodemeasurement,phaseanalysislightscattering(M3-PALS)

• Microrheology

• Proteinmeasurementsoftware

ZETASIZER NANO SERIESResearch Performance, operational Simplicity, Application Versatility

TheZetasizerNanoserieshasbeendesignedwithyouandyourrequirementsinmind.Youneedareliablesystemthatgivesconsistentlygoodperformance,thatiseasytouse,andcoversyourrangeofapplications.

Forcolloid,nanoparticleandmacromoleculecharacterization,ourcustomerstellusthattheZetasizerNanoinstrumentsarethemostuser-friendlysystemsavailable.Aswellasthiseaseofuse,theperformanceandconfidence

intheresultsisvalidatedbythefactthat94oftheWorld’stop100universitieshaveinvestedinaMalvernZetasizerNano*.

FromthetopoftherangeZetasizersystemsincorporatingNon-InvasiveBackscatter(NIBS)optics,totheaffordable90degreescatteringsystems,thereisaZetasizersuitableforeverylaboratoryinvolvedinthecharacterizationofnanoparticles,colloidaldispersions,polymerandproteinsolutions.

*Top100Universities:QSWorldUniversityRankings2011

www.malvern.com3 3

Within our range, there’s a Zetasizer Nano designed especially for you

Model Which system is right for your laboratory? Size Zeta potential

Molecular weight

Protein measurement

Microrheology

ZetasizerNanoZSP Thepremiumsystemfortheultimateincolloidandproteincharacterization

● ● ● ● optional

ZetasizerNanoZS TheWorld’smostwidelyusedDLSinstrument ● ● ● optional optional

ZetasizerNanoS Themodeldedicatedtosizemeasurement ● ●

ZetasizerNanoZ Themodeldedicatedtozetapotentialmeasurement ●

ZetasizerNanoZS90 Entry-levelsystemprovidingvalueandversatility ● ● ●

ZetasizerNanoS90 Entry-levelsizemeasurementsystem ● ●

InTRoDUcTIon To SIZE mEASUREmEnTBy DynAmIc lIghT ScATTERIng (DlS)

Laser

Smallparticles

Largeparticles

Laser

Samplecuvette

Scattered lightdensity

Correlationfunction

Sizedistribution


www.malvern.com4

Why DLS is suitable for your application

Thetechniqueisidealforthemeasurementofthesizeofcolloids,nanoparticlesandmolecules,withoutrequiringperturbationtomakethesamplesuitableforanalysis.

Fromthesize,anestimateofthemolecularweightcanbemade,whichhasthebenefitofbeingfastertoconfirmoligomericstatethansizeexclusionchromatography(SEC).

www.malvern.com5 5

How we measure size using Dynamic Light Scattering

Theprincipleofdynamiclightscatteringisthatfineparticlesandmoleculesthatareinconstantrandomthermalmotion,calledBrownianmotion,diffuseataspeedrelatedtotheirsize,smallerparticlesdiffusingfasterthanlargerparticles.ThespeedofBrownianmotionisalsodeterminedbythetemperature,thereforeprecisiontemperaturecontrolisessentialforaccuratesizemeasurement.

Tomeasurethediffusionspeed,thespecklepatternproducedbyilluminatingtheparticleswithalaserisobserved.Thescatteringintensityataspecificanglewillfluctuatewithtime,andthisisdetectedusingasensitiveavalanchephotodiodedetector(APD).Theintensitychangesareanalysedwithadigitalautocorrelatorwhichgeneratesacorrelationfunction.Thiscurvecanbeanalysedtogivethesizeandthesizedistribution.

Toproducehighqualitydata,theZetasizerNanoseriesisdesignedtoprovideoptimisedcomponentsateverystageinthemeasurementchainfromthelaserandtemperaturecontrol,throughtotheopticaldesignanddetector.

Benefits of using the Zetasizer Nano series for DLS measurements

• Researchgraderesultswiththeeaseofuseofaroutinesystem

• PatentedNIBSopticsensuresexceptionalperformance

• Samplemeasurementwithlittleornodilution

• Confidenceinresultsfromuniquedataqualitytestand‘Expertadvice’system

• Operatorindependenceensuredbyhighlyautomatedanalysissystem

• Automationoftemperaturetrends

• MPT-2AutotitratortoautomatepHandionicconcentrationtrends

Why are NIBS optics so beneficial for size measurement?

TheZetasizerNanoZSP,NanoZSandNanoSallusethepatentedNon-InvasiveBack-scatter(NIBS)technologywhichilluminatesalargernumberofparticlesandusesefficientfibredetection,giving100timesthesensitivityofconventionaloptics.Measuringalargernumberofparticlespracticallyeliminatesnumberfluctuations,givingamorestablesignal,andsignificantlyincreasingthelargestparticlesizethatcanbemeasured.

Focus on protein solutions

OneofthekeyapplicationareasfortheZetasizeristhecharacterisationsofproteinsinsolution.

• Molecularsizeandaggregationbehaviour

• MolecularweightbyDLSandSLS

• Secondvirialcoefficient,A2,B22

• DLSinteractionparameter,kD

• Proteinchargeandiso-electricpoint,PI

• Molecularconformation

www.malvern.com6

InTRoDUcTIon To ZETA POTENTIAL AND PROTEIN CHARGE

The importance of zeta potential and protein charge

Howdoyouapproachthedevelopmentofastabledispersionorassessproductshelflife?

Doyoumakelargenumbersoftestformulationsandsubjectthemtostresstests?

Ifso,youmaynotbeoptimisingthespeedofdevelopmentorensuringlongtermproductstability.

Thechargeacquiredbyaparticle,moleculeorproteininamediumarisesfromthesurfacechargeandtheconcentrationandtypeofionsinthesolution.Thischargeisresponsibleforthestabilityofthedispersionorsolution.

ThismeansthatthestabilitycanbemodifiedbyalteringthepH,theionicconcentration,thetypeofionsandbyusingadditivessuchassurfactantsandpolyelectrolytes.

Applications

• Reducingthedevelopmenttimeforstabledispersionsandproteinsolutions

• Understandingthereasonsforaproductstabilityorinstability,improvingproductshelflife

• Preventingproteinaggregateformation

• Increasingproteinconcentrationwhilemaintainingstability

• Optimizingflocculantdosagetoreducecostforwatertreatment.

How we measure zeta potential

Thechargeorzetapotentialofparticlesandmoleculesisdeterminedbymeasuringtheirvelocitywhiletheyaremovingduetoelectrophoresis.Particlesandmoleculesthathaveazetapotentialwillmigratetowardsanelectrodeifafieldisapplied.Thespeedtheymoveisproportionaltothefieldstrengthandtheirzetapotential.Ifweknowthefieldstrength,wesimplymeasurethespeedofmovement,usinglaserDopplerelectrophoresis,andthenapplyestablishedtheoriestocalculatethezetapotential.

Toimprovethesensitivityandaccuracyofthemeasurementsweuseatechniquecalledphaseanalysislightscattering(PALS).HoweverPALSonitsownonlyprovidesameanzetapotentialvalue,soourpatentedM3-PALSmulti-frequencymeasurementdeterminesthemeananddistributionduringthesamemeasurement.

Thewholemeasurementprocedureisautomatedtosimplifythemeasurementprocess.


Di�use layer

Electrical double layer

Slipping PlaneNanoparticle

Surface Potential

Zeta Potential

Distance from particle surface0

mV

Stem Layer

www.malvern.com7

Why measurement of zeta potential is useful

Thechoicesofmaterialsusedinaformulationmayberestrictedbyregulationsandalsohaveanimpactoncost.Knowledgeofthezetapotentialofparticlesinaformulationcanbeusedtomakelogicalchoicesaboutchemistryofaformulationinordertoselectthemostappropriatematerialstoprovidestabilityandimproveshelflife.

Zetapotentialcanalsobeusedtostudytheeffectofformulationcomponentsonotherbulkpropertiessuchasviscosity,inordertoachievelowerviscosityathigherconcentrationsforexample.

Summary of benefits

• Fullydisposablecelloptiontosimplifymeasurementandensureaccuracybyeliminatingcross-contamination

• Automatedmeasurementprocedure–nooperatorjudgementrequired–givesrepeatable,accurateresults

• M3-PALSensuresanaccuratezetapotentialdistribution

• SimpletousesoftwarewithSOPsandaselectionofreports

• Qualityreporttoreducetrainingrequirementandassistwithdatainterpretation

• ‘Expertadvice’systemtoassistwithoptimisingthemeasurementprocedure

Why measurement of protein charge is useful

Developingprotein-basedproducts,suchasproteintherapeuticsathighconcentration,demandsanunderstandingofinteractionsinarangeofsolventconditions,toensuretheyremainstable.

Thechargeonaproteinisoneofthefundamentalparametersthataffectsaspectsofproteinbehaviorsuchasaggregation,interactionwithmembranesandothersurfaces,ligandbindingaffinity,filtration,catalyticproperties,longtermstorage,crystallizationandprocessing.

Measurementofthechargecanprovidevaluableinformationthatassistswithimprovingaformulationinordertocontroltheseinteractions,predictstabilityandimproveshelflife

.

7

www.malvern.com8

SURfAcE ZETA PoTEnTIAl

What is surface zeta potential?

Surfacesincontactwithaliquidcontainingionscanhaveazetapotentialinthesamewayasdispersedparticlesandmolecules.Thiseffectivechargeonthesurfacewillattractorrepelmoleculesorparticlesintheliquid,andknowledgeofthiscanbeusedforavarietyofapplications,forexamplethedevelopmentofcontactlenses.

Measuring surface zeta potential

AnaccessorycellfortheZetasizerenablesasmallpieceoftheflatsampletobemountedbetweentwoelectrodes.Thepresenceofthematerialmodifiesthepatternofelectroosmosisbetweentheelectrodeswhenafieldisapplied,andthisisdetectedbythemeasurementofzetapotentialatanumberofdistancesfromthesurfaceofthematerial.Thesemeasurementsgivethezetapotentialatthesurface.


Applications

• Filterpapersusedtoremovedchargedmaterialssuchasbacteria

• Surfacemodificationofimplantstoimprovebiocompatibility

• Functionalizationofsurfacesusingchargedpolymers

• Layer-by-layerfabricationtomodifyoptical,electronicandcorrosion-resistantproperties

• Improvingthetimebetweenbackflushesofmembranefiltersusedforwaterfiltration

• Controllingliquidflowinmicrofluidicschannels

• Surfacemodificationtocontrolfrictionandadhesion.

Benefits of using the Zetasizer Nano for measuring surface zeta potential

• UsesastandardZetasizerNanowithasurfacecellaccessory

• Measurementprotocolandresultsareintegratedinthestandardsoftware

• Userisguidedthroughthemeasurementtosimplifyuse

• Aresultqualityreportisprovidedtoaiddatainterpretation.

9

InTRoDUcTIon To MOLECULAR WEIGHT MEASUREMENT

Measuring molecular weight

TheZetasizerNanoseriesenablesyoutomeasurethemolecularweightofmacromoleculesinsolutionusingthetechniqueofStaticLightScattering(SLS).TheSLStechniquerequiresthesystemtobesensitiveandexceptionallystable,sotheZetasizerhasbeendesignedtoprovidethisstability.

Molecular weight using the Zetasizer or Size Exclusion Chromatography?

• TheZetasizermeasurestheaveragemolecularweightofthesampleandisasimplewayofdeterminingoligomericcomposition

• Incomparison,SECseparatesthecomponentsofasamplebeforethecalculationofanaccurate

molecularweightdistribution.

How is molecular weight measured using SLS?

SLSrequiresthedeterminationofthescatteringintensityofanumberofknownconcentrationsofthemacromoleculeinsolution.

Theresultofthismeasurementisaweight-averagemolecularweight,andinadditionthesecondvirialcoefficient,A2orB22.Thisparameterisameasureofthesolubilityofthemolecule,soisanindicatorforsolutionstabilityandhasbeenusedinstudiesofproteincrystallization.

Specificallyforproteins,thesameseriesofmeasurementscanbeusedtodeterminetheDLSinteractionparameter,kD.

Benefits of using the Zetasizer Nano to measure molecular weight by SLS

• Smallamountofsamplerequired

• CalibrationonlyrequiresaknownpureliquidsuchasToluene

• Samplecanberecovered

• Secondvirialcoefficientcanbeusedtodetermineproteinsolubility

• CombinesizedatafromDLStodeterminestructurefactors.

0.000Concentration (g/mL)

Debye plot

0.001 0.002 0.003 0.004 0.005 0.0060.005

0.006

0.007

0.008

0.009

0.010 5000

4000

3000

2000

1000

KC

/RoP

(1/k

Da)

Corrected Scattering (kcps)

Debye plot for molecular weight measurement

www.malvern.com10

microrheology

Dynamiclightscattering(DLS)-basedopticalmicrorheologyusestracerprobeparticlestomeasuretherelationshipbetweenstressanddeformationinmaterials.Analogoustomechanicalrheometry,astressisapplied(byBrownianmotionofthetracerparticle)andthedeformationorstrainismeasured(throughchangesinthetracerposition).Thermally-drivenmotionofthetracerparticleisintimatelylinkedtotherheologicalpropertiesofthesuspendingfluid,andisverydifferentinapurelyviscousmedium(e.g.water)thanitisinaviscoelasticmedium(e.g.concentratedproteinsolution).Fromanalysisofthemeansquaredisplacement(MSD)oftheprobeparticles,rheologicalpropertiesofcomplexfluids,suchasviscosity,elasticmodulusG’andviscousmodulusG’’canbedetermined.

Probe particle indifferent environments

1

G’,

G”,

(Pa)

1

10

100

10 100

Frequency (rad/sec)

1000 10000

Example of a viscoelastic spectrum derived from MSD plot for a macromolecular solution

Viscous systeme.g. Solvent

Viscoelastic systeme.g. Protein/polymer solution

Time (s)

MSD

(m²)

Schematic of Mean Square Displacement (MSD) versus time

Viscousbehavior

Viscoelastic behavior


DLS Microrheologyprovides:

• Advancedrheologicalcharacterizationonverysmallsamplevolumesdownto12µL

• Viscoelasticcharacterizationoflowviscosity,weakly-structuredandhighlystrain-sensitivesamples–measurementswhichcanbeinaccessiblebymechanicalrheometrytechniques

• Accesstoveryhighfrequency(shorttime)dynamics-highlyrelevantfordilutesamples

Applications

• Rheologicalcharacterizationoftherapeuticproteinsandbiopolymersolutions

• Viscoelasticmeasurementsofproteinsolutionstoassessonsetofprotein-proteininteractionsandinsolubleaggregateformation

• Formulationdevelopmentandscreening

• Highfrequencyrheologyofdilutesystems-applicationorprocess-relevantcharacterization

• Monitorstructuredevelopmentincomplexfluidswithtimeortemperature,orstructurebreakdownondilution

www.malvern.com11

Cells

Awiderangeofcellsareavailableforsize,zetapotentialandmolecularweightmeasurement.Theseextendtheapplicationsthatcanbeaddressedtolowvolumes,higherconcentration,non-aqueousdispersantsandsolvents.

ZETASIZER nAno cEllS AnD oPTIonS

Options

• ChromatographyaccessorykittointegratewithanSECchromatographyunitasanabsolutesizedetector

• TheMPT-2AutotitratoranddegasserautomatesmeasurementsofzetapotentialandsizeasafunctionofpH,conductivityoranadditive

• Surfacezetapotentialaccessoryforthemeasurementofthezetapotentialofflatsurfaces

• TheSV-10ViscometertoensureaccurateDLSmeasurements

• Microrheologypackageenablesviscoelasticmeasurementsofsolutionsofproteinsandpolymers

• Hightemperatureoptionto120ºC

• Narrowbandfilteroptionforfluorescentsamples

• 21CFRpart11softwareassistswithER/EScompliance

• Researchsoftwareforthelightscatteringspecialist.

Standard materials• Thezetapotentialtestmaterialispre-

preparedtosimplifytheregularvalidationoftheoperationofthesystem

• SizestandardsareavailableasaNanosphererangefrom20nmto900nmwhichareverifiedagainstNISTcertifiedmaterials.

Moreinformationat:http://www.malvern.com/labeng/products/zetasizer/accessories/mpt2.htm

11

Celltype Description

DisposablefoldedcapillarycellSize,zetapotentialandproteinchargeavoidingcrosscontamination

DipcellZetapotentialforaqueousandnon-aqueousapplications

Highconcentrationcell Zetapotentialofsampleswithlittleornodilution

Surfacezetapotentialcell Zetapotentialofmaterialsurfaces

Disposablepolystyrenecell Lowcostsizecellforaqueoussamples

Disposablelowvolumepolystyrenecell Lowvolumesizecellforaqueoussamples

Glassorquartzcell SizeandMWcellforallsampletypes

Quartzflowcell Forusewithautotitratorandchromatographysystems

Ultralowvolumequartzcell Lowvolumesize,MWcellforallsampletypes

www.malvern.com

Parameters measured

Particleandmacromoleculesize,zetapotential,proteinchargesurfacezetapotential,molecularweight,secondvirialcoefficientA2,B22,DLSinteractionparameterkD,proteinaggregationandmicrorheologyproperties.

What is so special about these systems?

TheZetasizerseriesusesnovelandpatentedtechnologieswithintherangethatgiveunrivalledperformancewhileretainingeaseofuse.

• Non-InvasiveBack-Scatter(NIBS)increasessizingsensitivity,dynamicrangeandresultstability

• Thediffusionbarriertechniqueimprovesthestabilityofthechargemeasurementofproteins,anddramaticallyreducesthevolumeofsamplerequired.

• M3-PALSimproveszetapotentialsensitivityandprovidesadistributionaswellasameanvalue.

• Disposablecelloption,includingtheelectrodes,reducemeasurementtimeandensureaccuracybyeliminatingcrosscontamination

• Precisiontemperaturecontrolensuresaccurateandrepeatableresults.


non-InVASIVE BAcK-ScATTER (nIBS) SySTEmSZETASIZER nAno ZSP, nAno ZS and nAno S

12

Applications

• Developmentofnanoparticleproductionprocesses

• Shorteningformulationstabilitydevelopmentandtrials

• Improvingproductshelflife

• Investigatingthezetapotentialofsurfaces

• Optimisingflocculantdosageinwatertreatment

• Optimisingproteinformulationforstabilityandminimisingaggregates

• Determiningproteinaggregationpointtoinvestigatepurityandimproveshelflife.

Performance

• Thesensitivitytomeasuresmallanddilutesamples

• Thetechnologytomeasureatupto40%w/v

• Forwardscatteringanglefortheenhanceddetectionofaggregates

• Verysmallsamplevolumerequired

• Measurementinhighsaltandnon-aqueousmedia.

Features and benefits

• Littleornodilutionrequiredtosimplifysamplepreparation

• Widerangeofsamplesuitabilityfromnanoparticlestoemulsionsandmacromolecules

• MPT-2Autotitratortoimproveproductivity

• Eliminationofcross-contaminationusingdisposablecellstoimproveaccuracy

• Dataassessmentusingqualityreportand‘Expertadvice’system

• Useasachromatographydetectorforon-linesizemeasurement.

ThecombinationoftheNIBSandzetapotentialopticsprovidebothbackandforwardscatteringanglesforsizemeasurementtogiveanenhancedconcentrationrangeandsensitivitytoaggregates

APD Correlator

Fibre coupler

Laser

To Zetasizer software



Beamdump

Cell holder

Lens &translationassembly

Sizeattenuator

NIBSbackscatteroptics

Forwardscatteroptics

APD Correlator

Fibre coupler

Laser

Beamdump

Cell holderLens

Sizeattenuator

90 degreescattering

APD Correlator

Fibre coupler

Laser

Beamdump

Cell holder

Zeta attentuator

Zeta Compensationassembly

Lens

Reference beam

13

What makes the Zetasizer Nano ZS so popular?

TheNanoZShasbecomethemarketleadingsystembyprovidinggreatvalueandbysimplifyingthemeasurementofarangeofimportantparameterstohelpmakeyoumoreproductive.

TheNIBSopticsandM3-PALStechnologyensureawidesizeandconcentrationrange,whichreducesyourtimeandeffortforsamplepreparation.

Ahighdegreeofautomationofthemeasurementproceduremeansthatyoucanbeupandrunninginminutes,minimisingthetrainingrequirement.

ZETASIZER NANO ZS

ZETASIZER NANO ZSP

Get to grips with formulation development

TheZetasizerNanoZSPisthepremiummemberoftheZetasizerNanoseriesdesignedtoprovideexceptionalsensitivityforthemeasurementofsize,zetapotentialandmolecularweight.Thisisparticularlyimportantforapplicationswherethesampleissmall,diluteorscatterspoorly.Solutionsofproteinsareprimeexamplesofthis,andmeanthattheZetasizerisanalternativetotheuseofcapillaryelectrophoresisandisoelectricfocussingforchargemeasurement.

Moreover,theextendedproteintoolshavebeenre-designedtohelpyouunderstandmoreaboutyourformulationdevelopment.

Zetasizer Nano ZSP performance

• Higherpowerlaserandnewopticsgives10timeshigherzetapotentialsensitivitythantheZetasizerNanoZS–forthemeasurementofsmallermoredilutematerials

• Accuratelymeasuressmallersizesatlowerconcentrations

• Fastermeasurementtimesimprovestheefficiencyofuse.

14 www.malvern.com

TheZetasizerNanoSisdedicatedtothemeasurementofsizeandmolecularweight,usingthesamehighperformanceNIBSopticsastheZetasizerZS.

Zetasizer Nano S features and benefits

• Thesensitivitytomeasuresmallanddilutesamples

• Littleornodilutionrequiredtosimplifysamplepreparation

• Thetechnologytomeasureatupto40%w/v

• Minimumsamplevolumeof12µLforsizemeasurement

• Widerangeofsamplesuitabilityfromnanoparticlestoemulsionsandmacromolecules

• Automationusingautotitratortoimproveproductivity

• Dataassessmentusingqualityreportand‘Expertadvice’system

• Useasachromatographydetectorforon-linesizemeasurement.


ZETASIZER NANO S

ZETASIZER NANO Z

TheZetasizerNanoZisdedicatedtothemeasurementofzetapotential.

Zetasizer Nano Z features and benefits

• Measuresamplesupto40%w/v

• Minimumsamplevolumeof20µLforzetapotential

• Measurementinhighsaltandnon-aqueousmedia

• Widerangeofsamplesuitabilityfrom



• Dataassessmentusingqualityreportand‘Expertadvice’system.

APD Correlator

Fibre coupler

Laser




Beamdump

Cell holder


Sizeattenuator



APD Correlator

Fibre coupler

Laser

Beamdump

Cell holderLens

Sizeattenuator

90 degreescattering

APD Correlator

Fibre coupler

Laser

Beamdump

Cell holder

Zeta attentuator


Lens

Reference beam

Thezetapotentialopticslayoutusedforallzetapotentialandproteinchargemeasurements

Applications

• Colloidandemulsioncharacterization

• Pharmaceuticaldispersionsandemulsions

• Liposomesandvesicles

• Zetapotentialofparticlesandsurfaces

• Improvingink,tonerandpigmentperformance

• Optimisingflocculantdosageinwatertreatment.

Zetasizer Nano ZS90 features and benefits

• Thetechnologytomeasurezetapotentialatupto40%w/v(optionalhighconcentrationcellrequired)


• Minimumsamplevolumeof20µLforsizeandzetapotential

• Measurementinhighsaltandnon-aqueousmedia


• Dataassessmentusingqualityreportandexpertadvicetoreducetraining.

Zetasizer Nano S90 features and benefits

• Comparabilityofsizemeasurementswithother90degreeDLSsystems

• Minimumsamplevolumeof20µL

• Researchgradecorrelatortocoverawiderangeofrequirements


• Dataassessmentusingqualityreportandexpertadvicetoreducetraining

• Precisiontemperaturecontroltoensureaccuracyandrepeatability.

AffoRDABlE 90˚ OPTIC SYSTEMS ZETASIZER nAno ZS90 AnD nAno S90

What makes the Zetasizer Nano ZS90 and S90 ideal for routine applications?

Thesesystemsincorporate90ºscatteringopticsthatareanentry-levelsolutionforroutineapplicationsthatdon’trequiretheultimateinsensitivityandsizerange.

ThesoftwarebenefitsfromalltheeaseofusefeaturesanddevelopmentsincorporatedintherestoftheZetasizerNanorangetoensurecompatibilityinuseandtominimisetraining.

Zeta potential with the ZS90

ThezetapotentialdesignoftheZS90hasthesamecapabilityandspecificationasthemarketleadingsystem,theZetasizerNanoZS,ensuringexceptionaleaseofuseandthesamewideapplicationrange.ThismakestheZS90exceptionalvalueforapplicationsasdiverseasnanoparticles,emulsions,pigmentsandbiologicalcells.

APD Correlator

Fibre coupler

Laser




Beamdump

Cell holder


Sizeattenuator



APD Correlator

Fibre coupler

Laser

Beamdump

Cell holderLens

Sizeattenuator

90 degreescattering

APD Correlator

Fibre coupler

Laser

Beamdump

Cell holder

Zeta attentuator


Lens

Reference beam

The90ºscatteringsizeoptics

15

www.malvern.com


WoRlD-BEATIng SOFTWARE

The software is built using four criteria:

• Easeofmeasurement

• Easeofviewingdata

• Easeofdatainterpretation

• Compliancewithcurrentstandards

Software benefits include:

• Ahighdegreeofautomationsominimaltrainingisrequired

• Extensivefunctionalitywithoutcomplicatingthesoftware

• Dataqualityreporttogiveconfidenceintheresult

• Expertadvicesoftwaretohelpimprovethemeasurement

• Workspacecustomisationforprojects,sampletypesorindividualpersonnel

• FullintegrationofMPT-2AutotitratorforunattendedpHorionicstrengthtrendmeasurements

Optional software packages

• 21CFRpart11providessupportforElectronicRecords/ElectronicSignature(ER/ES)compliance

• Researchsoftwareforthelightscatteringspecialist

• Microrheologysoftwareforstudyingsolutionviscoeleasticproperties

• Proteinsoftwareforproteinmobility,chargeandinteractionstudies

16

Well-designedsoftwareisoneofthekeystotheeaseandefficientuseofasystem.TheexperiencegainedbyMalvernInstrumentsbydevelopingdynamiclightscatteringsystemsovermorethanthreedecadeshasensuredthattheversatilityincorporatedhasnotcompromisedeaseofuse.

Thesoftwareenablesyoutoextractthemaximuminformationfromyoursampleandpresentthedatainaclearway.Themeasurementproceduresarehighlyautomated,requiringminimaltrainingtouse.

www.malvern.com

17

TheZetasizerfamilyalsoincludestheZetasizerAPSandtheZetasizerµVsystemsthatareprimarilydesignedforthesizemeasurementofproteins.

Zetasizer APS

ToimproveDLSthroughputtheZetasizerAPSprovidesmeasurementautomationusing96or384wellplates.TheperformanceisidenticaltothebatchcapabilitiesoftheZetasizerµV,andensuresthatreliablesizemeasurementsandthermaltrendsofproteinscanbemadeunattended,improvingproductivity.

• Equivalentaccuracy,repeatabilityandsensitivityoftheZetasizerµV

• Multiplemeasurementtypesfromasingleplate,includingthermaltrends

• Platecoolingoptiontopreservesample

• Graphicalplatereportingtooltosimplifydatainterpretation

Moreinformationat:www.malvern.com/ZetasizerAPS

Zetasizer µV

Thisisadualfunctiondynamiclightscatteringsystem;ahighsensitivitysystemforproteinsolutionmeasurementsincuvettes,aswellasanSECdetector.ThesystemseamlesslyintegrateswithViscotekSECsystemsviathestandardOmniSECsoftware,aswellasthirdpartysystemsviatheOmniFACE.Thisgivesareal-timereadoutofbothproteinsizeandmolecularweighttogiveoligomerandaggregateidentificationwithoutcalibration.

• LightscatteringdetectorcompatiblewithanySECsystem

• AbsolutesizemeasurementbyDLSandSEC

• AbsolutemolecularweightofproteinsbySEC

• Measureincuvetteswithaminimumof2µLofsample

Moreinformationat:www.malvern.com/ZetasizeruV

oThER mEmBERS of THE ZETASIZER SERIES

www.malvern.com18

ZETASIZER nAno SPECIFICATIONS

Notes

*sampledependent**0.1°at25°C,0.2°at0°C,0.5°at90°C***Continalgorithminresearchsoftwareoption■ Optionalaccessory,purchasedseparately.

ZSP ZS S

ParametersmeasuredSize,Zetapotential,Molecularweight,A2

Size,Zetapotential,Molecularweight,A2

Size,Molecularweight,A2

Temperaturecontrolrange 0ºCto90ºC+/-0.1ºC** 0ºCto90ºC+/-0.1ºC** 0ºCto90ºC+/-0.1ºC**

Condensationcontrol Purgeusingdryair Purgeusingdryair Purgeusingdryair

Standardlaser 10mW,633nm 4mW,633nm 4mW,633nm

Correlator 25nsto8000s,max4000channels 25nsto8000s,max4000channels 25nsto8000s,max4000channels

Size

Absolutesensitivity(Toluenekcps) 300 150 150

Range(Maximumdiameter) 0.3nm-10microns* 0.3nm-10microns* 0.3nm-10microns*

Minsamplevolume 12µL 12µL 12µL

Minconcentration,protein 0.1mg/mL15kDaprotein 0.1mg/mL15kDaprotein 0.1mg/mL15kDaprotein

Minconcentration,forwardangle 1mg/mL15kDaprotein 10mg/mL66kDaprotein -

Maxconcentration 40%w/v* 40%w/v* 40%w/v*

Measurementangles(waterasdispersant) 13°+173° 13°+173° 173°

Analysisalgorithms***GeneralpurposeNNLS,multiple

narrowmodes,proteinGeneralpurposeNNLS,multiple


narrowmodes,protein

Zetapotential

Sensitivity 1mg/mL15kDaprotein 10mg/mL66kDaprotein -

Zetapotentialrange >+/-500mV >+/-500mV -

Mobilityrange >+/-20µ.cm/V.s >+/-20µ.cm/V.s -

Maximumsampleconcentration 40%w/v 40%w/v -

Minimumsamplevolume(usingdiffusionbarrier) 20µL 20µL -

Maximumsampleconductivity 200mS/cm 200mS/cm -

Signalprocessing M3-PALS M3-PALS -

Molecularweight

Molecularweightrange(estimatedfromDLS) <1000Da-2x107Da* <1000Da-2x107Da* <1000Da-2x107Da*

Molecularweightrange(Debyeplot) <1000Da-2x107Da* <1000Da-2x107Da* <1000Da-2x107Da*

Accessories

MPT-2Autotitratoranddegasser ■ ■ ■

Dipcell ■ ■ -

Highconcentrationcell ■ ■ -

Surfacezetapotentialcell(sample5mmx4mm) ■ ■ -

SV-10viscometer ■ ■ ■

Highpowerlaser,50mW,532nm - ■ ■

Hightemperaturerange,120ºC - ■ ■

Narrowbandfluorescencefilter ■ ■ ■

Chromatographyoption ■ ■ ■

21CFRpart11software ■ ■ ■

Microrheologysoftware ■ ■ -

Proteinmeasurementsoftware Included ■ ■

Researchsoftware ■ ■ ■

Dimensions

DxWxH,mm(Weight) 600x320x260(19kg) 600x320x260(19kg) 600x320x260(19kg)

Z ZS90 S90

ZetapotentialSize,Zetapotential,Molecularweight,A2

Size,Molecularweight,A2 Parametersmeasured

0ºCto90ºC+/-0.1ºC** 0ºCto90ºC+/-0.1ºC** 0ºCto90ºC+/-0.1ºC** Temperaturecontrolrange

Purgeusingdryair Purgeusingdryair Purgeusingdryair Condensationcontrol

4mW,633nm 4mW,633nm 4mW,633nm Standardlaser

25nsto8000s,max4000channels 25nsto8000s,max4000channels 25nsto8000s,max4000channels Correlator

Size

- 2 2 Absolutesensitivity(Toluenekcps)

- 0.3nm-5microns* 0.3nm-5microns* Range(Maximumdiameter)

- 20µL 20µL Minsamplevolume

- 10mg/mL15kDaprotein 10mg/mL15kDaprotein Minconcentration,protein

- - - Minconcentration,forwardangle

- Dilute Dilute Maxconcentration

- 13°+90° 90° Measurementangles(waterasdispersant)

-GeneralpurposeNNLS,multiple


narrowmodes,proteinAnalysisalgorithms***

Zetapotential

10mg/mL66kDaprotein 10mg/mL66kDaprotein - Sensitivity

>+/-500mV >+/-500mV - Zetapotentialrange

>+/-20µ.cm/V.s >+/-20µ.cm/V.s - Mobilityrange

40%w/v 40%w/v - Maximumsampleconcentration

20µL 20µL - Minimumsamplevolume(usingdiffusionbarrier)

200mS/cm 200mS/cm - Maximumsampleconductivity

M3-PALS M3-PALS - Signalprocessing

Molecularweight

- <1000Da-2x107Da* <1000Da-2x107Da* Molecularweightrange(estimatedfromDLS)

- <10,000Da-2x107Da* <10,000Da-2x107Da* Molecularweightrange(Debyeplot)

Accessories

■ ■ ■ MPT-2Autotitratoranddegasser

■ ■ - Dipcell

■ ■ - Highconcentrationcell

■ ■ - Surfacezetapotentialcell(sample5mmx4mm)

■ ■ ■ SV-10viscometer

■ ■ ■ Highpowerlaser,50mW,532nm

■ ■ ■ Hightemperaturerange,120ºC

■ ■ ■ Narrowbandfluorescencefilter

- ■ ■ Chromatographyoption

■ ■ ■ 21CFRpart11software

- - - Microrheologysoftware

■ - - Proteinmeasurementsoftware

■ ■ ■ Researchsoftware

Dimensions

600x320x260(19kg) 600x320x260(19kg) 600x320x260(19kg) DxWxH,mm(Weight)

Notes

*sampledependent**0.1°at25°C,0.2°at0°C,0.5°at90°C***Continalgorithminresearchsoftwareoption■ Optionalaccessory,purchasedseparately.

19

Malvern Instruments Limitedgrovewood Road, malvern, Worcestershire, UK, WR14 1XZ

Tel +44 1684 892456fax +44 1684 892789www.malvern.com

MalvernInstrumentsispartofSpectrisplc,thePrecisionInstrumentationandControlsCompany.

SpectrisandtheSpectrislogoareTradeMarksofSpectrisplc.

Allinformationsuppliedwithiniscorrectattimeofpublication.

MalvernInstrumentspursuesapolicyofcontinualimprovementduetotechnicaldevelopment.Wethereforereservetherighttodeviatefrominformation,descriptions,andspecificationsinthispublicationwithoutnotice.MalvernInstrumentsshallnotbeliableforerrorscontainedhereinorforincidentalorconsequentialdamagesinconnectionwiththefurnishing,performanceoruseofthismaterial.

Patents:

Non-InvasiveBackScatter(NIBS)EP0884580,DE19725211,US6016195,JP2911877

Multi-frequencyelectrophoresis(M3-PALS)UK2361772,EP1154266,US09/843339,JP2001-134510

Malvernandthe‘hills’logoandZetasizer,areInternationalTradeMarksownedbyMalvernInstrumentsLtd.

MRK1839-01

Malvern Solutions: Advanced technology made simple - distributor details

zetasizer nano series - dksh

Documents