zetasizer nano series - alfatest srl brochure serie zetasizer nano.pdf · key benefits of the nano...
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Performance, Simplicity, Versatility
Key benefits of the Nano Series
• Choiceoftechnologiesinacompactunitgivesexceptionalversatility
• Simplicityofoperationmeansminimaltrainingandrobustresults
• Highsensitivityfornanoparticles,proteinsandmacromolecules
• Disposablezetapotentialcuvetteforfast,accurateandeasymeasurements
• Highopticalqualityandtemperaturecontrolensuresaccuracyandrepeatability
• Novelmicrorheologyoptiontodetermineviscoelasticproperties
• MPT-2Autotitratoroptionforautomatedtrendmeasurements
There’s a Zetasizer Nano for every application
• Shortendevelopmenttimeforcolloidandemulsionformulations
• Improveformulationstability
• Assessproteinformulationstability
• Exploreproteinaggregationandoligomerizationstate
Technologies incorporated
• DualangleDynamicLightScattering(DLS)
• Non-InvasiveBack-Scatter(NIBS)
• StaticLightScattering(SLS)
• ElectrophoreticLightScattering(ELS)
• Mixedmodemeasurement,phaseanalysislightscattering(M3-PALS)
• Microrheology
• Proteinmeasurementsoftware
ZETASIZER NANO SERIESResearch Performance, operational Simplicity, Application Versatility
TheZetasizerNanoserieshasbeendesignedwithyouandyourrequirementsinmind.Youneedareliablesystemthatgivesconsistentlygoodperformance,thatiseasytouse,andcoversyourrangeofapplications.
Forcolloid,nanoparticleandmacromoleculecharacterization,ourcustomerstellusthattheZetasizerNanoinstrumentsarethemostuser-friendlysystemsavailable.Aswellasthiseaseofuse,theperformanceandconfidence
intheresultsisvalidatedbythefactthat94oftheWorld’stop100universitieshaveinvestedinaMalvernZetasizerNano*.
FromthetopoftherangeZetasizersystemsincorporatingNon-InvasiveBackscatter(NIBS)optics,totheaffordable90degreescatteringsystems,thereisaZetasizersuitableforeverylaboratoryinvolvedinthecharacterizationofnanoparticles,colloidaldispersions,polymerandproteinsolutions.
*Top100Universities:QSWorldUniversityRankings2011
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Within our range, there’s a Zetasizer Nano designed especially for you
Model Which system is right for your laboratory? Size Zeta potential
Molecular weight
Protein measurement
Microrheology
ZetasizerNanoZSP Thepremiumsystemfortheultimateincolloidandproteincharacterization
● ● ● ● optional
ZetasizerNanoZS TheWorld’smostwidelyusedDLSinstrument ● ● ● optional optional
ZetasizerNanoS Themodeldedicatedtosizemeasurement ● ●
ZetasizerNanoZ Themodeldedicatedtozetapotentialmeasurement ●
ZetasizerNanoZS90 Entry-levelsystemprovidingvalueandversatility ● ● ●
ZetasizerNanoS90 Entry-levelsizemeasurementsystem ● ●
InTRoDUcTIon To SIZE mEASUREmEnTBy DynAmIc lIghT ScATTERIng (DlS)
Laser
Smallparticles
Largeparticles
Laser
Samplecuvette
Scattered lightdensity
Correlationfunction
Sizedistribution
Performance, Simplicity, Versatility
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Why DLS is suitable for your application
Thetechniqueisidealforthemeasurementofthesizeofcolloids,nanoparticlesandmolecules,withoutrequiringperturbationtomakethesamplesuitableforanalysis.
Fromthesize,anestimateofthemolecularweightcanbemade,whichhasthebenefitofbeingfastertoconfirmoligomericstatethansizeexclusionchromatography(SEC).
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How we measure size using Dynamic Light Scattering
Theprincipleofdynamiclightscatteringisthatfineparticlesandmoleculesthatareinconstantrandomthermalmotion,calledBrownianmotion,diffuseataspeedrelatedtotheirsize,smallerparticlesdiffusingfasterthanlargerparticles.ThespeedofBrownianmotionisalsodeterminedbythetemperature,thereforeprecisiontemperaturecontrolisessentialforaccuratesizemeasurement.
Tomeasurethediffusionspeed,thespecklepatternproducedbyilluminatingtheparticleswithalaserisobserved.Thescatteringintensityataspecificanglewillfluctuatewithtime,andthisisdetectedusingasensitiveavalanchephotodiodedetector(APD).Theintensitychangesareanalysedwithadigitalautocorrelatorwhichgeneratesacorrelationfunction.Thiscurvecanbeanalysedtogivethesizeandthesizedistribution.
Toproducehighqualitydata,theZetasizerNanoseriesisdesignedtoprovideoptimisedcomponentsateverystageinthemeasurementchainfromthelaserandtemperaturecontrol,throughtotheopticaldesignanddetector.
Benefits of using the Zetasizer Nano series for DLS measurements
• Researchgraderesultswiththeeaseofuseofaroutinesystem
• PatentedNIBSopticsensuresexceptionalperformance
• Samplemeasurementwithlittleornodilution
• Confidenceinresultsfromuniquedataqualitytestand‘Expertadvice’system
• Operatorindependenceensuredbyhighlyautomatedanalysissystem
• Automationoftemperaturetrends
• MPT-2AutotitratortoautomatepHandionicconcentrationtrends
Why are NIBS optics so beneficial for size measurement?
TheZetasizerNanoZSP,NanoZSandNanoSallusethepatentedNon-InvasiveBack-scatter(NIBS)technologywhichilluminatesalargernumberofparticlesandusesefficientfibredetection,giving100timesthesensitivityofconventionaloptics.Measuringalargernumberofparticlespracticallyeliminatesnumberfluctuations,givingamorestablesignal,andsignificantlyincreasingthelargestparticlesizethatcanbemeasured.
Focus on protein solutions
OneofthekeyapplicationareasfortheZetasizeristhecharacterisationsofproteinsinsolution.
• Molecularsizeandaggregationbehaviour
• MolecularweightbyDLSandSLS
• Secondvirialcoefficient,A2,B22
• DLSinteractionparameter,kD
• Proteinchargeandiso-electricpoint,PI
• Molecularconformation
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InTRoDUcTIon To ZETA POTENTIAL AND PROTEIN CHARGE
The importance of zeta potential and protein charge
Howdoyouapproachthedevelopmentofastabledispersionorassessproductshelflife?
Doyoumakelargenumbersoftestformulationsandsubjectthemtostresstests?
Ifso,youmaynotbeoptimisingthespeedofdevelopmentorensuringlongtermproductstability.
Thechargeacquiredbyaparticle,moleculeorproteininamediumarisesfromthesurfacechargeandtheconcentrationandtypeofionsinthesolution.Thischargeisresponsibleforthestabilityofthedispersionorsolution.
ThismeansthatthestabilitycanbemodifiedbyalteringthepH,theionicconcentration,thetypeofionsandbyusingadditivessuchassurfactantsandpolyelectrolytes.
Applications
• Reducingthedevelopmenttimeforstabledispersionsandproteinsolutions
• Understandingthereasonsforaproductstabilityorinstability,improvingproductshelflife
• Preventingproteinaggregateformation
• Increasingproteinconcentrationwhilemaintainingstability
• Optimizingflocculantdosagetoreducecostforwatertreatment.
How we measure zeta potential
Thechargeorzetapotentialofparticlesandmoleculesisdeterminedbymeasuringtheirvelocitywhiletheyaremovingduetoelectrophoresis.Particlesandmoleculesthathaveazetapotentialwillmigratetowardsanelectrodeifafieldisapplied.Thespeedtheymoveisproportionaltothefieldstrengthandtheirzetapotential.Ifweknowthefieldstrength,wesimplymeasurethespeedofmovement,usinglaserDopplerelectrophoresis,andthenapplyestablishedtheoriestocalculatethezetapotential.
Toimprovethesensitivityandaccuracyofthemeasurementsweuseatechniquecalledphaseanalysislightscattering(PALS).HoweverPALSonitsownonlyprovidesameanzetapotentialvalue,soourpatentedM3-PALSmulti-frequencymeasurementdeterminesthemeananddistributionduringthesamemeasurement.
Thewholemeasurementprocedureisautomatedtosimplifythemeasurementprocess.
Performance, Simplicity, Versatility
Di�use layer
Electrical double layer
Slipping PlaneNanoparticle
Surface Potential
Zeta Potential
Distance from particle surface0
mV
Stem Layer
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Why measurement of zeta potential is useful
Thechoicesofmaterialsusedinaformulationmayberestrictedbyregulationsandalsohaveanimpactoncost.Knowledgeofthezetapotentialofparticlesinaformulationcanbeusedtomakelogicalchoicesaboutchemistryofaformulationinordertoselectthemostappropriatematerialstoprovidestabilityandimproveshelflife.
Zetapotentialcanalsobeusedtostudytheeffectofformulationcomponentsonotherbulkpropertiessuchasviscosity,inordertoachievelowerviscosityathigherconcentrationsforexample.
Summary of benefits
• Fullydisposablecelloptiontosimplifymeasurementandensureaccuracybyeliminatingcross-contamination
• Automatedmeasurementprocedure–nooperatorjudgementrequired–givesrepeatable,accurateresults
• M3-PALSensuresanaccuratezetapotentialdistribution
• SimpletousesoftwarewithSOPsandaselectionofreports
• Qualityreporttoreducetrainingrequirementandassistwithdatainterpretation
• ‘Expertadvice’systemtoassistwithoptimisingthemeasurementprocedure
Why measurement of protein charge is useful
Developingprotein-basedproducts,suchasproteintherapeuticsathighconcentration,demandsanunderstandingofinteractionsinarangeofsolventconditions,toensuretheyremainstable.
Thechargeonaproteinisoneofthefundamentalparametersthataffectsaspectsofproteinbehaviorsuchasaggregation,interactionwithmembranesandothersurfaces,ligandbindingaffinity,filtration,catalyticproperties,longtermstorage,crystallizationandprocessing.
Measurementofthechargecanprovidevaluableinformationthatassistswithimprovingaformulationinordertocontroltheseinteractions,predictstabilityandimproveshelflife
.
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SURfAcE ZETA PoTEnTIAl
What is surface zeta potential?
Surfacesincontactwithaliquidcontainingionscanhaveazetapotentialinthesamewayasdispersedparticlesandmolecules.Thiseffectivechargeonthesurfacewillattractorrepelmoleculesorparticlesintheliquid,andknowledgeofthiscanbeusedforavarietyofapplications,forexamplethedevelopmentofcontactlenses.
Measuring surface zeta potential
AnaccessorycellfortheZetasizerenablesasmallpieceoftheflatsampletobemountedbetweentwoelectrodes.Thepresenceofthematerialmodifiesthepatternofelectroosmosisbetweentheelectrodeswhenafieldisapplied,andthisisdetectedbythemeasurementofzetapotentialatanumberofdistancesfromthesurfaceofthematerial.Thesemeasurementsgivethezetapotentialatthesurface.
Performance, Simplicity, Versatility
Applications
• Filterpapersusedtoremovedchargedmaterialssuchasbacteria
• Surfacemodificationofimplantstoimprovebiocompatibility
• Functionalizationofsurfacesusingchargedpolymers
• Layer-by-layerfabricationtomodifyoptical,electronicandcorrosion-resistantproperties
• Improvingthetimebetweenbackflushesofmembranefiltersusedforwaterfiltration
• Controllingliquidflowinmicrofluidicschannels
• Surfacemodificationtocontrolfrictionandadhesion.
Benefits of using the Zetasizer Nano for measuring surface zeta potential
• UsesastandardZetasizerNanowithasurfacecellaccessory
• Measurementprotocolandresultsareintegratedinthestandardsoftware
• Userisguidedthroughthemeasurementtosimplifyuse
• Aresultqualityreportisprovidedtoaiddatainterpretation.
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InTRoDUcTIon To MOLECULAR WEIGHT MEASUREMENT
Measuring molecular weight
TheZetasizerNanoseriesenablesyoutomeasurethemolecularweightofmacromoleculesinsolutionusingthetechniqueofStaticLightScattering(SLS).TheSLStechniquerequiresthesystemtobesensitiveandexceptionallystable,sotheZetasizerhasbeendesignedtoprovidethisstability.
Molecular weight using the Zetasizer or Size Exclusion Chromatography?
• TheZetasizermeasurestheaveragemolecularweightofthesampleandisasimplewayofdeterminingoligomericcomposition
• Incomparison,SECseparatesthecomponentsofasamplebeforethecalculationofanaccurate
molecularweightdistribution.
How is molecular weight measured using SLS?
SLSrequiresthedeterminationofthescatteringintensityofanumberofknownconcentrationsofthemacromoleculeinsolution.
Theresultofthismeasurementisaweight-averagemolecularweight,andinadditionthesecondvirialcoefficient,A2orB22.Thisparameterisameasureofthesolubilityofthemolecule,soisanindicatorforsolutionstabilityandhasbeenusedinstudiesofproteincrystallization.
Specificallyforproteins,thesameseriesofmeasurementscanbeusedtodeterminetheDLSinteractionparameter,kD.
Benefits of using the Zetasizer Nano to measure molecular weight by SLS
• Smallamountofsamplerequired
• CalibrationonlyrequiresaknownpureliquidsuchasToluene
• Samplecanberecovered
• Secondvirialcoefficientcanbeusedtodetermineproteinsolubility
• CombinesizedatafromDLStodeterminestructurefactors.
0.000Concentration (g/mL)
Debye plot
0.001 0.002 0.003 0.004 0.005 0.0060.005
0.006
0.007
0.008
0.009
0.010 5000
4000
3000
2000
1000
KC
/RoP
(1/k
Da)
Corrected Scattering (kcps)
Debye plot for molecular weight measurement
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microrheology
Dynamiclightscattering(DLS)-basedopticalmicrorheologyusestracerprobeparticlestomeasuretherelationshipbetweenstressanddeformationinmaterials.Analogoustomechanicalrheometry,astressisapplied(byBrownianmotionofthetracerparticle)andthedeformationorstrainismeasured(throughchangesinthetracerposition).Thermally-drivenmotionofthetracerparticleisintimatelylinkedtotherheologicalpropertiesofthesuspendingfluid,andisverydifferentinapurelyviscousmedium(e.g.water)thanitisinaviscoelasticmedium(e.g.concentratedproteinsolution).Fromanalysisofthemeansquaredisplacement(MSD)oftheprobeparticles,rheologicalpropertiesofcomplexfluids,suchasviscosity,elasticmodulusG’andviscousmodulusG’’canbedetermined.
Probe particle indifferent environments
1
G’,
G”,
(Pa)
1
10
100
10 100
Frequency (rad/sec)
1000 10000
Example of a viscoelastic spectrum derived from MSD plot for a macromolecular solution
Viscous systeme.g. Solvent
Viscoelastic systeme.g. Protein/polymer solution
Time (s)
MSD
(m²)
Schematic of Mean Square Displacement (MSD) versus time
Viscousbehavior
Viscoelastic behavior
Performance, Simplicity, Versatility
DLS Microrheologyprovides:
• Advancedrheologicalcharacterizationonverysmallsamplevolumesdownto12µL
• Viscoelasticcharacterizationoflowviscosity,weakly-structuredandhighlystrain-sensitivesamples–measurementswhichcanbeinaccessiblebymechanicalrheometrytechniques
• Accesstoveryhighfrequency(shorttime)dynamics-highlyrelevantfordilutesamples
Applications
• Rheologicalcharacterizationoftherapeuticproteinsandbiopolymersolutions
• Viscoelasticmeasurementsofproteinsolutionstoassessonsetofprotein-proteininteractionsandinsolubleaggregateformation
• Formulationdevelopmentandscreening
• Highfrequencyrheologyofdilutesystems-applicationorprocess-relevantcharacterization
• Monitorstructuredevelopmentincomplexfluidswithtimeortemperature,orstructurebreakdownondilution
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Cells
Awiderangeofcellsareavailableforsize,zetapotentialandmolecularweightmeasurement.Theseextendtheapplicationsthatcanbeaddressedtolowvolumes,higherconcentration,non-aqueousdispersantsandsolvents.
ZETASIZER nAno cEllS AnD oPTIonS
Options
• ChromatographyaccessorykittointegratewithanSECchromatographyunitasanabsolutesizedetector
• TheMPT-2AutotitratoranddegasserautomatesmeasurementsofzetapotentialandsizeasafunctionofpH,conductivityoranadditive
• Surfacezetapotentialaccessoryforthemeasurementofthezetapotentialofflatsurfaces
• TheSV-10ViscometertoensureaccurateDLSmeasurements
• Microrheologypackageenablesviscoelasticmeasurementsofsolutionsofproteinsandpolymers
• Hightemperatureoptionto120ºC
• Narrowbandfilteroptionforfluorescentsamples
• 21CFRpart11softwareassistswithER/EScompliance
• Researchsoftwareforthelightscatteringspecialist.
Standard materials• Thezetapotentialtestmaterialispre-
preparedtosimplifytheregularvalidationoftheoperationofthesystem
• SizestandardsareavailableasaNanosphererangefrom20nmto900nmwhichareverifiedagainstNISTcertifiedmaterials.
Moreinformationat:http://www.malvern.com/labeng/products/zetasizer/accessories/mpt2.htm
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Celltype Description
DisposablefoldedcapillarycellSize,zetapotentialandproteinchargeavoidingcrosscontamination
DipcellZetapotentialforaqueousandnon-aqueousapplications
Highconcentrationcell Zetapotentialofsampleswithlittleornodilution
Surfacezetapotentialcell Zetapotentialofmaterialsurfaces
Disposablepolystyrenecell Lowcostsizecellforaqueoussamples
Disposablelowvolumepolystyrenecell Lowvolumesizecellforaqueoussamples
Glassorquartzcell SizeandMWcellforallsampletypes
Quartzflowcell Forusewithautotitratorandchromatographysystems
Ultralowvolumequartzcell Lowvolumesize,MWcellforallsampletypes
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Parameters measured
Particleandmacromoleculesize,zetapotential,proteinchargesurfacezetapotential,molecularweight,secondvirialcoefficientA2,B22,DLSinteractionparameterkD,proteinaggregationandmicrorheologyproperties.
What is so special about these systems?
TheZetasizerseriesusesnovelandpatentedtechnologieswithintherangethatgiveunrivalledperformancewhileretainingeaseofuse.
• Non-InvasiveBack-Scatter(NIBS)increasessizingsensitivity,dynamicrangeandresultstability
• Thediffusionbarriertechniqueimprovesthestabilityofthechargemeasurementofproteins,anddramaticallyreducesthevolumeofsamplerequired.
• M3-PALSimproveszetapotentialsensitivityandprovidesadistributionaswellasameanvalue.
• Disposablecelloption,includingtheelectrodes,reducemeasurementtimeandensureaccuracybyeliminatingcrosscontamination
• Precisiontemperaturecontrolensuresaccurateandrepeatableresults.
Performance, Simplicity, Versatility
non-InVASIVE BAcK-ScATTER (nIBS) SySTEmSZETASIZER nAno ZSP, nAno ZS and nAno S
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Applications
• Developmentofnanoparticleproductionprocesses
• Shorteningformulationstabilitydevelopmentandtrials
• Improvingproductshelflife
• Investigatingthezetapotentialofsurfaces
• Optimisingflocculantdosageinwatertreatment
• Optimisingproteinformulationforstabilityandminimisingaggregates
• Determiningproteinaggregationpointtoinvestigatepurityandimproveshelflife.
Performance
• Thesensitivitytomeasuresmallanddilutesamples
• Thetechnologytomeasureatupto40%w/v
• Forwardscatteringanglefortheenhanceddetectionofaggregates
• Verysmallsamplevolumerequired
• Measurementinhighsaltandnon-aqueousmedia.
Features and benefits
• Littleornodilutionrequiredtosimplifysamplepreparation
• Widerangeofsamplesuitabilityfromnanoparticlestoemulsionsandmacromolecules
• MPT-2Autotitratortoimproveproductivity
• Eliminationofcross-contaminationusingdisposablecellstoimproveaccuracy
• Dataassessmentusingqualityreportand‘Expertadvice’system
• Useasachromatographydetectorforon-linesizemeasurement.
ThecombinationoftheNIBSandzetapotentialopticsprovidebothbackandforwardscatteringanglesforsizemeasurementtogiveanenhancedconcentrationrangeandsensitivitytoaggregates
APD Correlator
Fibre coupler
Laser
To Zetasizer software
To Zetasizer software
To Zetasizer software
Beamdump
Cell holder
Lens &translationassembly
Sizeattenuator
NIBSbackscatteroptics
Forwardscatteroptics
APD Correlator
Fibre coupler
Laser
Beamdump
Cell holderLens
Sizeattenuator
90 degreescattering
APD Correlator
Fibre coupler
Laser
Beamdump
Cell holder
Zeta attentuator
Zeta Compensationassembly
Lens
Reference beam
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What makes the Zetasizer Nano ZS so popular?
TheNanoZShasbecomethemarketleadingsystembyprovidinggreatvalueandbysimplifyingthemeasurementofarangeofimportantparameterstohelpmakeyoumoreproductive.
TheNIBSopticsandM3-PALStechnologyensureawidesizeandconcentrationrange,whichreducesyourtimeandeffortforsamplepreparation.
Ahighdegreeofautomationofthemeasurementproceduremeansthatyoucanbeupandrunninginminutes,minimisingthetrainingrequirement.
ZETASIZER NANO ZS
ZETASIZER NANO ZSP
Get to grips with formulation development
TheZetasizerNanoZSPisthepremiummemberoftheZetasizerNanoseriesdesignedtoprovideexceptionalsensitivityforthemeasurementofsize,zetapotentialandmolecularweight.Thisisparticularlyimportantforapplicationswherethesampleissmall,diluteorscatterspoorly.Solutionsofproteinsareprimeexamplesofthis,andmeanthattheZetasizerisanalternativetotheuseofcapillaryelectrophoresisandisoelectricfocussingforchargemeasurement.
Moreover,theextendedproteintoolshavebeenre-designedtohelpyouunderstandmoreaboutyourformulationdevelopment.
Zetasizer Nano ZSP performance
• Higherpowerlaserandnewopticsgives10timeshigherzetapotentialsensitivitythantheZetasizerNanoZS–forthemeasurementofsmallermoredilutematerials
• Accuratelymeasuressmallersizesatlowerconcentrations
• Fastermeasurementtimesimprovestheefficiencyofuse.
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TheZetasizerNanoSisdedicatedtothemeasurementofsizeandmolecularweight,usingthesamehighperformanceNIBSopticsastheZetasizerZS.
Zetasizer Nano S features and benefits
• Thesensitivitytomeasuresmallanddilutesamples
• Littleornodilutionrequiredtosimplifysamplepreparation
• Thetechnologytomeasureatupto40%w/v
• Minimumsamplevolumeof12µLforsizemeasurement
• Widerangeofsamplesuitabilityfromnanoparticlestoemulsionsandmacromolecules
• Automationusingautotitratortoimproveproductivity
• Dataassessmentusingqualityreportand‘Expertadvice’system
• Useasachromatographydetectorforon-linesizemeasurement.
Performance, Simplicity, Versatility
ZETASIZER NANO S
ZETASIZER NANO Z
TheZetasizerNanoZisdedicatedtothemeasurementofzetapotential.
Zetasizer Nano Z features and benefits
• Measuresamplesupto40%w/v
• Minimumsamplevolumeof20µLforzetapotential
• Measurementinhighsaltandnon-aqueousmedia
• Widerangeofsamplesuitabilityfrom
• Automationusingautotitratortoimproveproductivity
• Eliminationofcross-contaminationusingdisposablecellstoimproveaccuracy
• Dataassessmentusingqualityreportand‘Expertadvice’system.
APD Correlator
Fibre coupler
Laser
To Zetasizer software
To Zetasizer software
To Zetasizer software
Beamdump
Cell holder
Lens &translationassembly
Sizeattenuator
NIBSbackscatteroptics
Forwardscatteroptics
APD Correlator
Fibre coupler
Laser
Beamdump
Cell holderLens
Sizeattenuator
90 degreescattering
APD Correlator
Fibre coupler
Laser
Beamdump
Cell holder
Zeta attentuator
Zeta Compensationassembly
Lens
Reference beam
Thezetapotentialopticslayoutusedforallzetapotentialandproteinchargemeasurements
Applications
• Colloidandemulsioncharacterization
• Pharmaceuticaldispersionsandemulsions
• Liposomesandvesicles
• Zetapotentialofparticlesandsurfaces
• Improvingink,tonerandpigmentperformance
• Optimisingflocculantdosageinwatertreatment.
Zetasizer Nano ZS90 features and benefits
• Thetechnologytomeasurezetapotentialatupto40%w/v(optionalhighconcentrationcellrequired)
• Eliminationofcross-contaminationusingdisposablecellstoimproveaccuracy
• Minimumsamplevolumeof20µLforsizeandzetapotential
• Measurementinhighsaltandnon-aqueousmedia
• Automationusingautotitratortoimproveproductivity
• Dataassessmentusingqualityreportandexpertadvicetoreducetraining.
Zetasizer Nano S90 features and benefits
• Comparabilityofsizemeasurementswithother90degreeDLSsystems
• Minimumsamplevolumeof20µL
• Researchgradecorrelatortocoverawiderangeofrequirements
• Automationusingautotitratortoimproveproductivity
• Dataassessmentusingqualityreportandexpertadvicetoreducetraining
• Precisiontemperaturecontroltoensureaccuracyandrepeatability.
AffoRDABlE 90˚ OPTIC SYSTEMS ZETASIZER nAno ZS90 AnD nAno S90
What makes the Zetasizer Nano ZS90 and S90 ideal for routine applications?
Thesesystemsincorporate90ºscatteringopticsthatareanentry-levelsolutionforroutineapplicationsthatdon’trequiretheultimateinsensitivityandsizerange.
ThesoftwarebenefitsfromalltheeaseofusefeaturesanddevelopmentsincorporatedintherestoftheZetasizerNanorangetoensurecompatibilityinuseandtominimisetraining.
Zeta potential with the ZS90
ThezetapotentialdesignoftheZS90hasthesamecapabilityandspecificationasthemarketleadingsystem,theZetasizerNanoZS,ensuringexceptionaleaseofuseandthesamewideapplicationrange.ThismakestheZS90exceptionalvalueforapplicationsasdiverseasnanoparticles,emulsions,pigmentsandbiologicalcells.
APD Correlator
Fibre coupler
Laser
To Zetasizer software
To Zetasizer software
To Zetasizer software
Beamdump
Cell holder
Lens &translationassembly
Sizeattenuator
NIBSbackscatteroptics
Forwardscatteroptics
APD Correlator
Fibre coupler
Laser
Beamdump
Cell holderLens
Sizeattenuator
90 degreescattering
APD Correlator
Fibre coupler
Laser
Beamdump
Cell holder
Zeta attentuator
Zeta Compensationassembly
Lens
Reference beam
The90ºscatteringsizeoptics
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Performance, Simplicity, Versatility
WoRlD-BEATIng SOFTWARE
The software is built using four criteria:
• Easeofmeasurement
• Easeofviewingdata
• Easeofdatainterpretation
• Compliancewithcurrentstandards
Software benefits include:
• Ahighdegreeofautomationsominimaltrainingisrequired
• Extensivefunctionalitywithoutcomplicatingthesoftware
• Dataqualityreporttogiveconfidenceintheresult
• Expertadvicesoftwaretohelpimprovethemeasurement
• Workspacecustomisationforprojects,sampletypesorindividualpersonnel
• FullintegrationofMPT-2AutotitratorforunattendedpHorionicstrengthtrendmeasurements
Optional software packages
• 21CFRpart11providessupportforElectronicRecords/ElectronicSignature(ER/ES)compliance
• Researchsoftwareforthelightscatteringspecialist
• Microrheologysoftwareforstudyingsolutionviscoeleasticproperties
• Proteinsoftwareforproteinmobility,chargeandinteractionstudies
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Well-designedsoftwareisoneofthekeystotheeaseandefficientuseofasystem.TheexperiencegainedbyMalvernInstrumentsbydevelopingdynamiclightscatteringsystemsovermorethanthreedecadeshasensuredthattheversatilityincorporatedhasnotcompromisedeaseofuse.
Thesoftwareenablesyoutoextractthemaximuminformationfromyoursampleandpresentthedatainaclearway.Themeasurementproceduresarehighlyautomated,requiringminimaltrainingtouse.
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TheZetasizerfamilyalsoincludestheZetasizerAPSandtheZetasizerµVsystemsthatareprimarilydesignedforthesizemeasurementofproteins.
Zetasizer APS
ToimproveDLSthroughputtheZetasizerAPSprovidesmeasurementautomationusing96or384wellplates.TheperformanceisidenticaltothebatchcapabilitiesoftheZetasizerµV,andensuresthatreliablesizemeasurementsandthermaltrendsofproteinscanbemadeunattended,improvingproductivity.
• Equivalentaccuracy,repeatabilityandsensitivityoftheZetasizerµV
• Multiplemeasurementtypesfromasingleplate,includingthermaltrends
• Platecoolingoptiontopreservesample
• Graphicalplatereportingtooltosimplifydatainterpretation
Moreinformationat:www.malvern.com/ZetasizerAPS
Zetasizer µV
Thisisadualfunctiondynamiclightscatteringsystem;ahighsensitivitysystemforproteinsolutionmeasurementsincuvettes,aswellasanSECdetector.ThesystemseamlesslyintegrateswithViscotekSECsystemsviathestandardOmniSECsoftware,aswellasthirdpartysystemsviatheOmniFACE.Thisgivesareal-timereadoutofbothproteinsizeandmolecularweighttogiveoligomerandaggregateidentificationwithoutcalibration.
• LightscatteringdetectorcompatiblewithanySECsystem
• AbsolutesizemeasurementbyDLSandSEC
• AbsolutemolecularweightofproteinsbySEC
• Measureincuvetteswithaminimumof2µLofsample
Moreinformationat:www.malvern.com/ZetasizeruV
oThER mEmBERS of THE ZETASIZER SERIES
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ZETASIZER nAno SPECIFICATIONS
Notes
*sampledependent**0.1°at25°C,0.2°at0°C,0.5°at90°C***Continalgorithminresearchsoftwareoption■ Optionalaccessory,purchasedseparately.
ZSP ZS S
ParametersmeasuredSize,Zetapotential,Molecularweight,A2
Size,Zetapotential,Molecularweight,A2
Size,Molecularweight,A2
Temperaturecontrolrange 0ºCto90ºC+/-0.1ºC** 0ºCto90ºC+/-0.1ºC** 0ºCto90ºC+/-0.1ºC**
Condensationcontrol Purgeusingdryair Purgeusingdryair Purgeusingdryair
Standardlaser 10mW,633nm 4mW,633nm 4mW,633nm
Correlator 25nsto8000s,max4000channels 25nsto8000s,max4000channels 25nsto8000s,max4000channels
Size
Absolutesensitivity(Toluenekcps) 300 150 150
Range(Maximumdiameter) 0.3nm-10microns* 0.3nm-10microns* 0.3nm-10microns*
Minsamplevolume 12µL 12µL 12µL
Minconcentration,protein 0.1mg/mL15kDaprotein 0.1mg/mL15kDaprotein 0.1mg/mL15kDaprotein
Minconcentration,forwardangle 1mg/mL15kDaprotein 10mg/mL66kDaprotein -
Maxconcentration 40%w/v* 40%w/v* 40%w/v*
Measurementangles(waterasdispersant) 13°+173° 13°+173° 173°
Analysisalgorithms***GeneralpurposeNNLS,multiple
narrowmodes,proteinGeneralpurposeNNLS,multiple
narrowmodes,proteinGeneralpurposeNNLS,multiple
narrowmodes,protein
Zetapotential
Sensitivity 1mg/mL15kDaprotein 10mg/mL66kDaprotein -
Zetapotentialrange >+/-500mV >+/-500mV -
Mobilityrange >+/-20µ.cm/V.s >+/-20µ.cm/V.s -
Maximumsampleconcentration 40%w/v 40%w/v -
Minimumsamplevolume(usingdiffusionbarrier) 20µL 20µL -
Maximumsampleconductivity 200mS/cm 200mS/cm -
Signalprocessing M3-PALS M3-PALS -
Molecularweight
Molecularweightrange(estimatedfromDLS) <1000Da-2x107Da* <1000Da-2x107Da* <1000Da-2x107Da*
Molecularweightrange(Debyeplot) <1000Da-2x107Da* <1000Da-2x107Da* <1000Da-2x107Da*
Accessories
MPT-2Autotitratoranddegasser ■ ■ ■
Dipcell ■ ■ -
Highconcentrationcell ■ ■ -
Surfacezetapotentialcell(sample5mmx4mm) ■ ■ -
SV-10viscometer ■ ■ ■
Highpowerlaser,50mW,532nm - ■ ■
Hightemperaturerange,120ºC - ■ ■
Narrowbandfluorescencefilter ■ ■ ■
Chromatographyoption ■ ■ ■
21CFRpart11software ■ ■ ■
Microrheologysoftware ■ ■ -
Proteinmeasurementsoftware Included ■ ■
Researchsoftware ■ ■ ■
Dimensions
DxWxH,mm(Weight) 600x320x260(19kg) 600x320x260(19kg) 600x320x260(19kg)
Z ZS90 S90
ZetapotentialSize,Zetapotential,Molecularweight,A2
Size,Molecularweight,A2 Parametersmeasured
0ºCto90ºC+/-0.1ºC** 0ºCto90ºC+/-0.1ºC** 0ºCto90ºC+/-0.1ºC** Temperaturecontrolrange
Purgeusingdryair Purgeusingdryair Purgeusingdryair Condensationcontrol
4mW,633nm 4mW,633nm 4mW,633nm Standardlaser
25nsto8000s,max4000channels 25nsto8000s,max4000channels 25nsto8000s,max4000channels Correlator
Size
- 2 2 Absolutesensitivity(Toluenekcps)
- 0.3nm-5microns* 0.3nm-5microns* Range(Maximumdiameter)
- 20µL 20µL Minsamplevolume
- 10mg/mL15kDaprotein 10mg/mL15kDaprotein Minconcentration,protein
- - - Minconcentration,forwardangle
- Dilute Dilute Maxconcentration
- 13°+90° 90° Measurementangles(waterasdispersant)
-GeneralpurposeNNLS,multiple
narrowmodes,proteinGeneralpurposeNNLS,multiple
narrowmodes,proteinAnalysisalgorithms***
Zetapotential
10mg/mL66kDaprotein 10mg/mL66kDaprotein - Sensitivity
>+/-500mV >+/-500mV - Zetapotentialrange
>+/-20µ.cm/V.s >+/-20µ.cm/V.s - Mobilityrange
40%w/v 40%w/v - Maximumsampleconcentration
20µL 20µL - Minimumsamplevolume(usingdiffusionbarrier)
200mS/cm 200mS/cm - Maximumsampleconductivity
M3-PALS M3-PALS - Signalprocessing
Molecularweight
- <1000Da-2x107Da* <1000Da-2x107Da* Molecularweightrange(estimatedfromDLS)
- <10,000Da-2x107Da* <10,000Da-2x107Da* Molecularweightrange(Debyeplot)
Accessories
■ ■ ■ MPT-2Autotitratoranddegasser
■ ■ - Dipcell
■ ■ - Highconcentrationcell
■ ■ - Surfacezetapotentialcell(sample5mmx4mm)
■ ■ ■ SV-10viscometer
■ ■ ■ Highpowerlaser,50mW,532nm
■ ■ ■ Hightemperaturerange,120ºC
■ ■ ■ Narrowbandfluorescencefilter
- ■ ■ Chromatographyoption
■ ■ ■ 21CFRpart11software
- - - Microrheologysoftware
■ - - Proteinmeasurementsoftware
■ ■ ■ Researchsoftware
Dimensions
600x320x260(19kg) 600x320x260(19kg) 600x320x260(19kg) DxWxH,mm(Weight)
Notes
*sampledependent**0.1°at25°C,0.2°at0°C,0.5°at90°C***Continalgorithminresearchsoftwareoption■ Optionalaccessory,purchasedseparately.
19
Malvern Instruments Limitedgrovewood Road, malvern, Worcestershire, UK, WR14 1XZ
Tel +44 1684 892456fax +44 1684 892789www.malvern.com
MalvernInstrumentsispartofSpectrisplc,thePrecisionInstrumentationandControlsCompany.
SpectrisandtheSpectrislogoareTradeMarksofSpectrisplc.
Allinformationsuppliedwithiniscorrectattimeofpublication.
MalvernInstrumentspursuesapolicyofcontinualimprovementduetotechnicaldevelopment.Wethereforereservetherighttodeviatefrominformation,descriptions,andspecificationsinthispublicationwithoutnotice.MalvernInstrumentsshallnotbeliableforerrorscontainedhereinorforincidentalorconsequentialdamagesinconnectionwiththefurnishing,performanceoruseofthismaterial.
Patents:
Non-InvasiveBackScatter(NIBS)EP0884580,DE19725211,US6016195,JP2911877
Multi-frequencyelectrophoresis(M3-PALS)UK2361772,EP1154266,US09/843339,JP2001-134510
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