yellowstone‘s brave heart „layla“ · / yellowstone's brave heart “leyla”...
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Yellowstone‘s Brave Heart „Layla“
YellowstoneAustralian Shepherds
Yellowstone‘s Brave Heart „Layla“
J e n n i f e r & M i r k o Wa l t e rA m E h r e n m a l 1 4 | 4 1 5 6 4 K a a r s t | N R W | G e r m a n y
Te l . : + 4 9 ( 0 ) 2 1 3 1 / 1 7 6 0 8 2 8M o b i l : + 4 9 ( 0 ) 1 6 3 / 0 8 1 8 1 5 0
E m a i l : i n f o @ Ye l l o s t o n e A u s s i e s . d eW e b : w w w .Ye l l o w s t o n e A u s s i e s . d e
Hip / Ellbow (HD & ED)
DNA
AUSTRALIAN SHEPHERD CLUB OF AMERICA, INC.
presents to the A USTRALIAN SHEPHERD YELLOWSTONE'S BRA VE HEART DNA- VP
Established 1957
Registration No. E184190
.. M,u, n,
THE AWARD OF DNA-VP has been verified and recorded in the DNA Database.
having fully completed the requirements of this club
On this date March 22, 2014
OWNER JENNIFER WALTER
March 24. 2014 ^S^fryns/G JM.CÁ&WA~ Issued this date ASCA President
Therion International, LLC Australian Shepherd Club of America DNA Certification
Date Reported: 3/22/2014
Offspring Yellowstone's Brave Heart
Internal ID Registry Number Sex A140324 E184190 Female
Sire: Highlands Heartbreaker Luke Registry Number E176726
Dam: Blue Mountains The One 'n' Only Registry Number El 79130
Individual qualifies as offspring of the indicated Sire and Dam
1.9A
190 194
Sire DNA Genotype constructed by Biopsytec (Adjusted by Therion International, LLC)
AH125 AH126 UK101 UK109 UK111 UK118 UK133 CPH3 CXX403 CXX2137
108 108 205 205 139 144 110 115 91 91 124 132 161 165 168 182 272 272 162 166
LEI007
272 274
1.9A
Offspring DNA Genotype constructed by Therion International, LLC
AH125 AH126 UK101 UK109 UK111 UK118 UK133 CPH3 CXX403 CXX2137
94 108 199 205 127 144 110 115 77 91 124 124 161 161 182 0 272 276 166 170
LEI007
272 0
1.9A
Dam DNA Genotype constructed by Therion International, LLC
AH125 AH126 UK101 UK109 UK111 UK118 UK133 CPH3 CXX403 CXX2137
94 108 199 0 127 137 110 0 77 91 124 124 0 0 184 184 272 276 170 170
LEI007
272 0
Augen / Eyes
Gebiss / Teeth
Multi Drug Resistance Gene (MDR1)
Slovgen s.r.o. , Diagnostické laboratórium, Dúbravská cesta 21, 841 04 Bratislava 4, tel : 02/5930 7434, 0905 550 916, fax : 5930 7434
________________________________________________________________________________
PROTOCOL OF DNA ANALYSIS FOR DETECTION OF INHERITED DISEASES
Submitter of analysis : Jennifer Mewes Date of sampling : 20.08.2013 Am Ehrenmal 14 Date of samples receipt: 06.09.2013 41564 Kaarst, Germany Date of analysis : 10.09.2013
Breed/name Tattoo or RFID
id/Certificate of origin Laboratory code Type of
analysis Result
/ Yellowstone's Brave Heart “Leyla”
953000010134010 130906/H2068 MDR1 N/N Non-affected
The samples and results of analysis are stored in a database under the lab code 130906/H2068. Hints: Genotype MDR1 +/+ or N/N (non-affected): Both genes, inherited from both mother and father are undamaged (healthy).
Genotype MDR1 +/– or N/P (carrier): Subjects with confirmed heterozygous genotype are carriers. Defective gene can be transmitted to offspring. Unwanted side effects are unlikely to occur but cannot be excluded. Genotype MDR1 –/– or P/P (affected): Particular caution is necessary in case an individual is diagnosed MDR1 -/- genotype. Treatment with certain drugs in this case can cause significant problems in some cases lethal neurotoxic reaction.
Notice: This protocol applies exclusively to the sample and the data that were supplied by the submitter. DNA analysis concerns only the above mentioned disease. No information regarding the customer as well as purpose and results of the analysis will be provided to third parties. In Bratislava 11.09.2013 Ing. Marcela Bieliková, PhD.
Heat Shock Fac tor Pro tein 4 (HSF4)
B e f u n d - N r . : 1 3 0 2 - W - 0 4 7 1 6 H M k I ^k ^ \ D ^ ^ i \ L \LABOR FÜR KLINISCHE DIAGNOSTIK GMBH & CO. KG
D i e V e r a n t w o r t u n g f ü r d i e R i c h t i g k e i t d e r A n g a b e n z u d e ne i n g e s a n d t e n P r o b e n l i e g t b e i m E i n s e n d e r .
H e r e d i t ä r e r K a t a r a k t ( H C ) - P C R
E r g e b n i s : G e n o t y p : N / N ( H S F 4 f r e i )I n t e r p r e t a t i o n : D e r u n t e r s u c h t e H u n d i s t r e i n e r b i g { h o m o z y g o t ) f ü rd a s n o r m a l e G e n . D i e s e r H u n d t r ä g t n i c h t d i e M u t a t i o n , d i e a l su r s ä c h l i c h f ü r d i e s e F o r m d e s K a t a r a k t s b e s c h r i e b e n w u r d e .E s b e s t e h t k e i n R i s i k o , d a s s d e r H u n d a n d i e s e r F o r m d e sh e r e d i t ä r e n K a t a r a k t s ( H C ) e r k r a n k t - E r g i b t n u r d a s n o r m a l e G e n a ns e i n e N a c h k o m m e n w e i t e r . U n t e r s u c h t w u r d e d i e b i s h e r b e k a n n t eM u t a t i o n . D a s E r g e b n i s g i l t n u r f ü r d a s i m L a b o r e i n g e g a n g e n eU n t e r s u c h u n g M a t e r i a l u n d f ü r d i e R a s s e n A u s t r a l i a n S h e p h e r d u n dW ä l l e r .
D a s E r g e b n i s g i l t n u r f ü r d a s i m L a b o r e i n g e g a n g e n e P r o b e n m a t e r i a l .D i e V e r a n t w o r t u n g f ü r d i e R i c h t i g k e i t d e r A n g a b e n z u d e n e i n g e s a n d t e nP r o b e n l i e g t b e i m E i n s e n d e r . G e w ä h r l e i s t u n g s v e r p f l i c h t u n g e n k ö n n e nn i c h t ü b e r n o m m e n w e r d e n . S c h a d e n s e r s a t z v e r p f l i c h t u n g e n s i n d , s o w e i tg e s e t z l i c h z u l ä s s i g , a u f d e n R e c h n u n g s w e r t d e r d u r c h g e f ü h r t e nU n t e r s u c h u n g / e n b e s c h r ä n k t .
W e i t e r e G e n v e r ä n d e r u n g e n , d i e e b e n f a l l s d i e A u s p r ä g u n g d e rE r k r a n k u n g / M e r k m a l e b e e i n f l u s s e n k ö n n e n , k ö n n e n n i c h ta u s g e s c h l o s s e nw e r d e n . D i e U n t e r s u c h u n g / e n e r f o l g t e / n n a c h d e m d e r z e i t i g e n w i s s e ns c h a f t l i c h e n K e n n t n i s s t a n d .
D a s L a b o r i s t f ü r d i e a u f d i e s e m B e f u n d a u f g e f ü h r t e n U n t e r s u c h u n g e na k k r e d i t i e r t n a c h D I N E N I S O 1 7 0 2 5 ( D - P L - 1 3 1 8 6 - 0 1 ) . ( a u s g e n o m m e nP a r t n e r l a b o r - L e i s t u n g e n ) .
Z u c h t v e r b a n d s r a b a t t e w u r d e n b e r ü c k s i c h t i g t !
* * * E N D E d e s B e f u n d e s * * *
F r . D i p l , - B i o l . B ä r b e l G u n r e b e nA b t . M o l e k u l a r b i o l o g i e
* : A u s f ü h r u n g d u r c h P a r t n e r l a b o r
Sleubenstraße 4 • 97688 Bad Kissingen • Tel.: 0971/72020 ♦ Fax: 0971/68546 • Geschäftsführender Gesellschafter: LAB0KL1N Vefwaltungs-GmbH • RG. Schweinfurt HRA 3631Bankverbindung; Postgiroamt NQmt>erg (BLZ 76010085) Kto.-Nr. 3060851, BCEE Luxembourg Kto.-Nr. 1507/3600-5, Salzburger Sparkasse Kio.-Nr. 122762 • e-Mail: [email protected]
Steuernr.: 205/167/00301 • USMD DE206897824
SEP 08 2010 is 33PM HP LRSERJET 3EQG 03717536303 p.l
HAX-Nummer: 0-02651/494181 4 £ -
Tierfcherspeutische Praxis Manuela und Peter Mayer »lacht igallBftweg 17 56729 Ettlingen Deutschland
ißPr.SnftStfGBTOMGSBSffüHO
fi*£tradnuaB*rt 10Q8-P-Q4104 Srobeaeiagang omt 35-08-2010 Probenentnahme am: 23-08-20X0
untersucoungsmaterial: Blut Datan -des Patienten: Hund Australian Shepherd "Blue mountain silk» Geschlecht : weiblich Alter: %2 Monate _ Name dee Tierbesitzers: Craê
R«i«restwart
Tiemasoe» »Blue Jtermfcain S i l k stocking Sentara" Heriditarer Katarakt HC: Ergebnis.- Genotyp:. N/K (HSP4 frei) interpretation; Der untersuchte Hund ist reinerbig {homozygot} fur das normale Gen. Dieser Hund trägt nicht die Mutation, die als ursächlich für diese Form des Katarakts beschrieben wurde. Es besteht kein Riaifco, dasa der Hund an̂ dieser Por« dee hereditären: Katarakts (HC) erkrankt. Er gibt nur das normale Gen an seine Nachkommer welter •. ^fcérsucht w»*de die bisher bekannte Muta-tion ; Bas Bräebnis gilt nur für das im Labor eingegangene Unterauchungsmaterial und für die Rassen Australian Sheperd und Waller.
ZB-Nummer s iös*ii Chip-Nummer ---Tat o -Summer BBrJSO0O720üOO003?0
Die Verantwortung für die Richtigkeit der Angaben su den einge-sandten Proben liegt beim Einsender. Gewährleistungsverpfilchtungen können nicht übettonnrnöen werden.
- 1 -
sMtwttwiii r • mea Bau KMMM • w.: oa n / n s 1?6 • N * W n /1% ia va • »MA hto«v*ttí««n¿,
VBT8CBE6W OMtftclvrft mibe«tt«n*MrKah^eSiS^I^ Q.USSáL R»«,«,»
eMail : heikegierOweb.de LABOR FÜR KLINISCHE DIAGNOSTIK GMBH & CO. KC
UVBOKLIN GmbH&CoKG . P o s t f a c h JL810 . 97668 Bad K i s s i n g e n
Tierarztpraxis Dres. Domagk / Geks Huckelhovener Str. 17 41836 Hückelhoven-Doveren Deutschland
Untersuchungsbefund Nr.: 0904-S-23149 Datum Eingang: 12-06-2007 Datum Befund:
Angaben zum Patienten:
Probenentnahme : Patientenbesitzer : Probenmaterial :
Hund Australian Shepherd männlich 09-06-2007 Gier, Heike Blut
Messgrößen Ist Referenzwert
Nachbestellung vom 29.04.09 zu Befund-Nr. 0706-S-33300
Name: "C.H. Very Welcome Chenius Cathares"
(HC) PCR Hereditärer Katarakt Ergebnis HC Ergebnis: Genotyp: N/N (HSF4 frei)
» Interpretation: Der untersuchte Hund ist reinerbig (homozygot) für das normale Gen. Dieser Hund trägt nicht die Mutation, die als ursächlich für diese Form des Katarakts beschrieben wurde. Es besteht kein Risiko, dass der Hund an dieser Form des hereditären Katarakts (HC) erkrankt. Er gibt nur das normale Gen ar seine Nachkommen weiter. Das Ergenbis gilt nur für das im Labor eingegangene Untersuchung Material und für die Rasse Australian Shepherd.
ZB-Nummer: Chip-Nummer: Täto-Nummer:
E 134880
2 DPH 105 Das Labor ist für die oben stehende/n Untersuchung/en akkreditiert nach DIN EN ISO 17025 (AKS-PL-20922). Die Verantwortung für die Richtigkeit der Angaben zu den eingesandten Proben liegt beim Einsender. Gewährleistungsverpflichtungen können nicht übernommen
Steubenstraße 4 • 87688 Bad Kissingsn • Tel:0971/72020 • Fax: 0971/68548 • GeschättstChrender Gesellschafter; LABOKUN Vemaitungs-GmbH • RG. Schwainlurt HRA 3631 Bankverbindung: Postgtroam! Nürnberg (BLZ 76010085) Kto -Nr. 3060651. BCEE Luxembourg Kto.44r.lS07/3S00-S. Satzburger Spartesse wo.-Nr. 122 /62 • e-Mail: [email protected]
Brachyurie
Slovgen s.r.o. , Diagnostické laboratórium, Dúbravská cesta 21, 841 04 Bratislava 4, tel : 02/5930 7434, 0905 550 916, fax : 5930 7434
________________________________________________________________________________
DNA ANALYSIS PROTOCOL FOR DETECTION OF HEREDITARY DISEASES
Submitter of analysis : Jennifer Walter Date of sampling : 03.03.2014 Am Ehrenmal 14 Date of samples receipt: 06.03.2014 41564 Kaarst, Germany Date of analysis : 10.03.2014
Breed/name Tattoo or RFID id/ Certificate of origin
Laboratory code Type of analysis
Result
/ Yellowstone's Brave Heart “Layla” 953000010134010 140306/K0731 Bob tail/ Short tail
N/N (C/C) Non-affected
The results of analysis are stored in a database under the lab code 140306/K0731.
Hints: Bob tail –congenital/naturally short-tail, The naturally occurring short-tail phenotype has dominant expression
The short-tail phenotype is caused by the mutation C189G in exon 1 of T-gene affecting the DNA-binding ability of T-protein. Dogs carrying the mutation in T-gene have natural short tails, but can differ in many respects. Some dogs have no tail and some have only half tails.
N/N (C/C) – homozygous individual carrying two C alleles – non – affected (clear) N/A (C/G) – heterozygous carrier carrying one allele C and one G, congenital/naturally short-tail
Notice: This protocol applies exclusively to the sample and the data that were supplied by the submitter. DNA analysis concerns only the above mentioned disease. No information regarding the customer as well as purpose and results of the analysis will be provided to third parties. In Bratislava 11.03.2014 Ing. Marcela Bieliková, PhD.
Collie Eye Anomalie (CEA)
Slovgen s.r.o. , Diagnostické laboratórium, Dúbravská cesta 21, 841 04 Bratislava 4, tel : 02/5930 7434, 0905 550 916, fax : 5930 7434
________________________________________________________________________________
PROTOCOL OF DNA ANALYSIS FOR DETECTION OF INHERITED DISEASES
Submitter of analysis : Jennifer Mewes Date of sampling : 20.08.2013 Am Ehrenmal 14 Date of samples receipt: 06.09.2013 41564 Kaarst, Germany Date of analysis : 10.09.2013
Breed/name Tattoo or RFID
id/Certificate of origin Laboratory code Type of
analysis Result
/ Yellowstone's Brave Heart “Leyla”
953000010134010 130906/H2068 CEA R/R Non-affected
The samples and results of analysis are stored in a database under the lab code 130906/H2068. Hints: Genotype CEA R/R – healthy subject – non-affected Both genes, inherited from both male and female are unaffected. That means that the subject has both alleles healthy. Genotype CEA R/r - carrier Subjects with confirmed heterozygous CEA R/r genotype are carriers. Gene mutation can be transmitted to offspring. Genotype CEA r/r – affected subject
The subject is a homozygote with r/r genotype, which inherited the affected allele from both parents and thus is affected by the disease.
Notice: This protocol applies exclusively to the sample and the data that were supplied by the submitter. DNA analysis concerns only the above mentioned disease. No information regarding the customer as well as purpose and results of the analysis will be provided to third parties. In Bratislava 11.09.2013 Ing. Marcela Bieliková, PhD.
Pro gres sive Re ti naa tro phie (PRA)
Slovgen s.r.o. , Diagnostické laboratórium, Dúbravská cesta 21, 841 04 Bratislava 4, tel : 02/5930 7434, 0905 550 916, fax : 5930 7434
________________________________________________________________________________
PROTOCOL OF DNA ANALYSIS FOR DETECTION OF INHERITED DISEASES
Submitter of analysis : Jennifer Mewes Date of sampling : 20.08.2013 Am Ehrenmal 14 Date of samples receipt: 06.09.2013 41564 Kaarst, Germany Date of analysis : 10.09.2013
Breed/name Tattoo or RFID
id/Certificate of origin Laboratory code Type of
analysis Result
/ Yellowstone's Brave Heart “Leyla”
953000010134010 130906/H2068 PRA-prcd N/N Non-affected
The samples and results of analysis are stored in a database under the lab code 130906/H2068. Hints: Genotype PRA-prcd N/N – healthy subject – non-affected Both genes, inherited from both male and female are unaffected. That means that the subject has both alleles healthy. Genotype PRA-prcd N/A – carrier Subjects with confirmed heterozygous N/A genotype are carriers. Gene mutation can be transmitted to offspring. Genotype PRA-prcd A/A – affected subject
The subject is a homozygote with A/A genotype, which inherited the affected allele from both parents and thus is affected by the disease.
Notice: This protocol applies exclusively to the sample and the data that were supplied by the submitter. DNA analysis concerns only the above mentioned disease. No information regarding the customer as well as purpose and results of the analysis will be provided to third parties. In Bratislava 11.09.2013 Ing. Marcela Bieliková, PhD.
Dilute
Befund-Nr.: 1303-W-08686 LABOKLIN LABOR FÜR KLINISCHE DIAGNOSTIK GMBH & CO. KG
werden. Die Untersuchung/en erfolgte/n nach dem derzeitigen wissen-schaftlichen Kenntnisstand.
Das Labor ist für die auf diesem Befund aufgeführten Untersuchungen akkreditiert nach DIN EN ISO 17025 (D-PL-13186-01). (ausgenommen Partnerlabor-Leistungen).
Neuronale Ceroid Lipofuszinose (NCL) - PCR Ergebnis : Genotyp N/N Interpretation: Der untersuchte Hund ist reinerbig für das intakte Gen. Er ist kein Träger des Gens für Neuronale Zeroid-lipofuszinose. Das Tier ist genetisch gesund und wird die von der Mutation ausgelösten Symptome nicht ausprägen. An die Nachkommen wird nur das intakte Gen weitergegeben. Das Ergebnis gilt nur für das im Labor eingegangene Untersuchungsmaterial und für die Rasse Border Collie, Dackel, English Setter, Tibet Terrier, Australian Shepherd und American Staffordshire Terrier.
D-Lokus (Dilution, Fellfarbverdünnunq) - PCR
.Ergebnis: Genotyp D/D Interpretation: Der untersuchte Hund ist kein Anlageträger des Farbverdünnungsallels Dilution. Der Hund kann an seine Nachkommen nur das Wildtypallel (D), nicht aber das Verdünnungsallel (d) weitergeben.
Das Ergebnis gilt nur für das im Labor eingegangene Untersuchungsmaterial.
*prcd-PRA (Partnerlabor) - PCR
Ergebnis: Genotyp: N/N (A, frei) Interpretation: Der untersuchte Hund ist reinerbig (homozygot) für das normale Gen. Dieser Hund trägt nicht die prcd-PRA-Mutation, die als ursächlich für diese Form der PRA beschrieben wurde. Es besteht kein Risiko, dass der Hund an dieser Form der iprcd-PRA erkrankt. Der untersuchte Hund kann mit jedem beliebigen Hund verpaart werden, . da hierbei kein Risiko für betroffene Nachkommen besteht.
Das Ergebnis gilt für das eingesandte Untersuchungsmaterial und die Rassen Australian Cattle Dog, American Cocker Spaniel, American Eskimo, Chesapeake Bay Retriever, Chinese Chrested, Spanischer Wasserhund, Golden Retriever, Kleinpudel, English Cocker Spaniel, Entlebucher Sennenhund, Finnischer Lapphund,
Steubenstraße 4 • 97688 Bad Kissingen • Tel.: 0971/72020 • Fax: 0971/68546 • Geschäftslührender Gesellschafter: LABOKLIN Verwaltungs-GmbH • RG. Schweinfurt HRA 3631 Bankverbindung: Postgiroamt Nürnberg (BLZ 760 10085) Kto.-Nr. 3060851. BCEE Luxembourg Kto.-Nr. 1507/3600-5, Salzburger Sparkasse Kto.-Nr. 122762 • e-Mail: infoeiaboklin.de
Steuernr.: 205/167/00301 • USt.lD DE206897824
DEGENERATIVE MYE LO PA THIE (DM)
Slovgen s.r.o., Diagnostické laboratórium, Dúbravská cesta 21, 841 04 Bratislava 4, tel : 02/5930 7434, 0905 550 916, fax : 5930 7434
DNA ANALYSIS PROTOCOL FOR DETECTION OF HEREDITARY DISEASES
Submitter of analysis Jennifer Walter Am Ehrenmal 14 41564 Kaarst, Germany
Date of sampling : Date of samples receipt: Date of analysis :
03.03.2014 06.03.2014 07.03.2014
Breed/name
/ Yellowstone's Brave Heart "Layla"
Tattoo or RFID id/ Certificate of origin 953000010134010
Laboratory code
140306/K0730
Type of analysis
DM
The results of analysis are stored in a database under the lab code 140306/K0730.
Result
N/N (G/G) Non-affected |
Hints: DM -degenerative myelopathy DM N/N (G/G) - homozygous individual carrying two G alleles - non - affected (clear) DM N/DM (G/A) - heterozygous carrier carrying one allele G and one A DM DM/DM (AJA) - homozygous at risk/affected individual - both alleles are A
Notice: This protocol applies exclusively to the sample and the data that were supplied by the submitter. DNA analysis concerns only the above mentioned disease. No information regarding the customer as well as purpose and results of the analysis will be provided to third parties.
In Bratislava 10.03.2014 Ing. Marcela Bieliková, PhD.
Pedigree
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Sex ; FEMALE m.Sßoi3 & í •..
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ASCA CERTIFIES THAT IT ACeüFiÄTEtY MAINTAINS THE
: GENEAlOGiCAL-ÍNFORIWATlÓN. VWHICH IS FUkNI5HEErT0;ITBY BREEDEFtS, THE lilNEAG&OF AfcREGISTERED DOG CAN ,BE DETERMINED .WITH ' . ÖEftTAItlTY BYDNA TESTiNG. ' 'SpONSÔREb BY ASCA
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