xray crystallography - ifm · terms used in xray crystallography ... • assymmetric unit •...
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Xray crystallography
Lasse Mårtensson, IFM-Chemistry
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Ribosome
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• © 2006 • Academic Press
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• © 2006 • Academic Press
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Diffraction pattern from a protein obtained by xray crystallography
• © 2006 • Academic Press
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Electrondensity map
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• © 2006 • Academic Press
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• © 2006 • Academic Press
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• © 2006 • Academic Press
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• © 2006 • Academic Press
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• © 2006 • Academic Press
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• Requirements: • Pure protein of high concentration 10-50
mg/ml AND… • Protein crystals How to obtain protein crystals??
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• © 2006 • Academic Press
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• © 2006 • Academic Press
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• © 2006 • Academic Press
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• © 2006 • Academic Press
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• © 2006 • Academic Press
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Crystallization robot
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Terms used in Xray crystallography
• Crystal system • Spacegroup • Unit cell • Assymmetric unit • Miller indices hkl • Braggs law • Ewald sphere