Xray crystallography
Lasse Mårtensson, IFM-Chemistry
Ribosome
• © 2006 • Academic Press
• © 2006 • Academic Press
Diffraction pattern from a protein obtained by xray crystallography
• © 2006 • Academic Press
Electrondensity map
• © 2006 • Academic Press
• © 2006 • Academic Press
• © 2006 • Academic Press
• © 2006 • Academic Press
• © 2006 • Academic Press
• Requirements: • Pure protein of high concentration 10-50
mg/ml AND… • Protein crystals How to obtain protein crystals??
• © 2006 • Academic Press
• © 2006 • Academic Press
• © 2006 • Academic Press
• © 2006 • Academic Press
• © 2006 • Academic Press
Crystallization robot
Terms used in Xray crystallography
• Crystal system • Spacegroup • Unit cell • Assymmetric unit • Miller indices hkl • Braggs law • Ewald sphere