work done a centre for soft and condensed
TRANSCRIPT
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Work done a Centre for Soft and
Condensed Matter.
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Fabrication of glass nano-pipettes and micro-pipettes
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Pore diameter: 104.7 nm Shrunk diameter: 74.36 nm
Pore diameter: 88.62 nm Shrunk diameter: 54.78 nm
Shrinking of glass nanopores
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Device characterization
I-V curves for 5 salt buffer concentrations. Using the I-V curves calculate the
conductance.
Experimental Set up
and reading
Pore diameter = 176.1 nm
Molarity Resistance (MΩ) Irms (Noise) pA Beta value (β) Alpha Value (α)
1M 2.13 8.47 0.1 0.5
0.1M 7.75 3.94 0.1 0.5
50mM 11.34 4.33 0.1 0.5
1mM 87.53 3.91 0.1 0.5
100µM 95.99 3.41 0.1 0.5
1µM 98.15 3.87 0.1 0.5
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Results
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Flow cell designed for imaging pipettes
under 20X and 40X objective
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Micro fluidic cell designed for pressure and
diffusion experiments through pipettes.
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Flow cell designed for visualizing electrical and pressure
driven translocation events through pipettes.
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Oscillation of 1 micron polystyrene beads in
alternating electric field.
Position A Position B
Polystyrene
bead
Function
generator
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Calculating the velocity of 1 micron polystyrene
beads oscillating in alternating electric field.
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1a 1b 1c 1d
5a 5b 5c 5d 5e
Translocation of Polystyrene beads through
sub micron glass pipettes
Fig (1a-1d) translocation of 15 micron beads through 50 micron pipette
Fig (5a-5e) translocation of fluorescent 15 micron beads through 30 micron pipette
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2a 2b 2c 2d
3a 3b 3c 3d 3e
4a 4b 4c 4d
Fig (2a-2d); (3a-3e); (4a-4d) – Translocation of 3 micron beads through 5 micron pipette
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Pressure driven Translocation of 450 nm polystyrene beads through a 900 nm pipette,
image taken under 40X objective.
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Pressure driven diffusion through pipettes to track the
trajectory of polystyrene beads at the mouth of the
micropipette
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Pore diameter: 1.6 µm
Translocation and visualization of polystyrene beads
(500 nm and 1 µm) under alternating electrical stimulus.
Flow cell used for visualizing and performing translocation events.
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Visualizing a single electrical translocation event, of a 1 micron polystyrene bead oscillating
under alternating field at 3V.
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