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What we’ve learned about sour rot: An update on research Wendy McFadden-Smith Tender Fruit & Grape IPM Specialist CCOVI Lecture March 14, 2012

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Page 1: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

What we’ve learned about sour

rot: An update on research

Wendy McFadden-Smith

Tender Fruit & Grape IPM Specialist

CCOVI Lecture

March 14, 2012

Page 2: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Acknowledgements

• Ontario Grape and Wine Research Inc.

• Niagara Peninsula Fruit and Vegetable Growers

Association

• Vincor Canada

• Schenck Greenhouses and Farms Ltd.

Page 3: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Acknowledgements

• Dr. Debra Inglis (Co-researcher, CCOVI, Brock University)

• Cristina Huber (Ph.D. candidate, Brock University)

• Lisa Dowling (undergrad thesis)

• Rhiannon Plant (undergrad thesis)

• Summer students

– Ed Gigliotti, Patrick Schenck, Teia Clement, Rachel Kvas, Lisa Indris, Izabelle Sagan

• Dr. Ai-Lin Beh (Inglis lab)

• Shiri Sauday (Inglis lab)

• Linda Tremblay (juice analysis, CCOVI)

• Paula Haag, Dan O’Gorman & Dr. Peter Sholberg, AAFC Summerland (molecular techniques)

• Dr. Keith Seifert, AAFC (ID of non-pathogenic organisms)

• Agri-Corp (sample collection)

Page 4: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Acknowledgements

• BASF Canada

• N.M. Bartlett

• Biosafe Systems

• Forterra Inc.

• NORAC Concepts Inc.

• Plant Products

• Bioworks Inc.

• Innovotech

Page 5: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 6: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 7: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 8: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Relationship between Sour Rot Severity and VA

P. noir, 2011

0.00

0.05

0.10

0.15

0.20

0.25

0.30

Vo

lati

le a

cid

ity (

g A

A/L

)

0

1

2

3

4

5

6

So

ur

rot

severi

ty (

%)

Sour rot

Page 9: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Relationship between Sour Rot Severity and VA

P. noir, 2011

0.00

0.05

0.10

0.15

0.20

0.25

0.30

Vo

lati

le a

cid

ity (

g A

A/L

)

0

1

2

3

4

5

6

So

ur

rot

severi

ty (

%)

VA Sour rot

Page 10: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

What’s causing it????

Page 11: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

What’s causing it?

• Samples of sour rotted berries

• Flamed to remove surface organisms

Page 12: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Isolations from rotted berries, 2010

0

10

20

30

40

50

60

70

80

90

100

B Pnoir C Gam C Ries Cr Niag Cr

SauvBl

G Niag G Ries H Baco I Ries K Ries L Pnoir L Ries M Pnoir S Pnoir W Ries

AA Bacteria Hanseniaspora Bacillus Aureobasidium

O O

Page 13: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Isolations from rotted P. noir berries, 2011

0

10

20

30

40

50

60

70

80

90

100

AAbacteria Hanseniaspora Candida Pichia Bacillus Aureobasidium

Page 14: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Isolations from rotted Chardonnay berries, 2011

0

10

20

30

40

50

60

70

80

90

100

AAbacteria Hanseniaspora Candida Pichia Bacillus Aureobasidium

Page 15: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Isolations from rotted Riesling berries, 2011

0

10

20

30

40

50

60

70

80

90

100

AAbacteria Hanseniaspora Candida Pichia Bacillus Aureobasidium

Page 16: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Rating scale for sour rot

Page 17: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Pathogencity of Organisms

0

0.5

1

1.5

2

2.5

3

3.5

4

Negative Gluconobacter Acetobacter Hanseniaspora

uvarum

Candida

zemplinina

Complex

Page 18: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Pathogencity of Organisms

0

0.5

1

1.5

2

2.5

3

3.5

4

4.5

AA Bacteria

Candida

zemplina

Hanseniaspora uvarum

Page 19: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Why does it happen?

Page 20: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Factors that promote sour rot

• Tight clusters/Thin skins

– Varieties Affected

• Pinot noir, Pinot gris,

Gamay, Chardonnay,

Riesling,

Gewurztraminer, Baco

noir

Page 21: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Factors that Promote Sour Rot

• Favoured by

– Physical damage

• Powdery mildew

Gadoury et al., 2007

Page 22: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Factors that Promote Sour Rot

• Clusters infected with bunch rot are more prone to

infection by sour rot

Page 23: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 24: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Factors that Promote Sour Rot

• But

– Frequently found sour rot without bunch rot sporulation

– Frequently found sour rot in areas of clusters (shoulders)

where no berry squeeze occurred

– Very weak correlation (r= 0.028 - 0.147) between severity of

bunch rot and sour rot 2008, 2010, 2011

Page 25: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Factors that Promote Sour Rot

• Grape Berry Moth

– Bunch rot frequently associated with GBM injury

– But a problem in vineyards with low GBM pressure

Page 26: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 27: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 28: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 29: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Factors that Promote Sour Rot

• Vinegar flies attracted by volatile

compounds released during berry

degradation

• Vector sour-rot organisms

– passive transport by adults

– eggs laid near base of berry where it

pulls away from stem

– transmitted throughout cluster

during larval stages

– larvae carry sour rot organisms in

their gut.

Page 30: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 31: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 32: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Factors that Promote Sour Rot

0

5

10

15

20

21 28 5 12 19 27

0.0

1.0

2.0

3.0

4.0

5.0

Frost

Sept Oct

2009 Riesling

Brix Avg Temp (C)

Page 33: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Factors that Promote Sour Rot

0

5

10

15

20

25

30

35

40

10 17 24 31 7 14 21 30

2010 Riesling

Avg Temp (C)

Brix

Aug Sept

Page 34: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Factors that Promote Sour Rot

0

5

10

15

20

25

30

35

20 22 24 26 28 30 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 1 3 5 7 9 11 13 15Aug Sept Oct

2011 Riesling

Avg Temp (C)

Brix

TA

Page 35: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Factors that Promote Sour Rot

0

5

10

15

20

25

30

20 21 22 23 24 25 26 27 28 29 30 31 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Aug Sept

2011 P. noir

Avg Temp (C)

Brix

TA

Page 36: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 37: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 38: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Brix vs Sour rot Development

P. noir, 2011

0

0.5

1

1.5

2

2.5

3

3.5

4

4.5

9.7° 11.2° 13.4° 15.5°

Page 39: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

What can we do about it?

Page 40: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Sour Rot Management

• Reduce berry injury

• Eliminate causal organisms

Page 41: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Reduce Berry Injury

• Loosen grape clusters

– Reduce berry squeeze

– Thicker cuticle

Page 42: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Treatments to loosen clusters

• Fruiting zone sprays @ 100 gal/A

• Gibberellic Acid

– 5, 10, 20 ppm

– (6.7, 13.4, 26.8 oz/100 US gal)

– Prebloom, 50-80% bloom, bloom + 7 days

• Prohexidione-Ca

– High, medium and low rate

– (9.7, 4.8 and 2.4 oz/100 US gal)

• Stimplex

– 38, 48, 68 oz/A at 80% bloom.

Page 43: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Cluster Compactness Scale

Zabadal and Dittmer

Page 44: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Reduce Injury

• Loosen grape clusters

– Bloom basal leaf removal

• 6 basal leaves removed at trace bloom

• starves clusters for photosynthate and fewer flowers set

fruit.

• looser cluster with fewer berries

Page 45: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Before Bloom Leaf Removal

Page 46: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

After Bloom Leaf Removal

Page 47: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Untreated

No leaf removal

Veraison

Page 48: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Leaf removal

at bloom

Veraison

Page 49: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Pea-sized berry

Leaf removal

Veraison

Page 50: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Veraison

Leaf removal

Veraison

Page 51: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Effect of Bloom Treatments

on Cluster Looseness, 2009

0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

Unt

reat

ed

Bas

al le

af p

ull

Pro

hex-

Ca

high

Pro

hex-

Ca

med

Pro

hex-

Ca

low

GA 5

ppm

GA 5

ppm

2X

GA 1

0 pp

m p

rebl

GA 1

0 pp

m

GA 1

0 pp

m 2

X

GA 2

0 pp

m

Clu

ste

r L

oo

se

ne

ss

Riesling Pinot noir

Page 52: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Effect of Bloom Treatments

on Incidence of Sour Rot, Riesling, 2009

0

5

10

15

20

25

30

Pro

d X 4

5 g

Pro

d X 9

0 g

Pro

d X 1

80 g

GA 5

ppm

GA 5

ppm

2X

GA 1

0 pp

m

GA 1

0 pp

m 2

X

GA 2

0 pp

m

Stim

plet

2.8

L

Stim

plex

3.5

L

Stim

plex

5L

Bloom

leaf

Unt

reat

ed

Inc

ide

nc

e (

%)

Very low sour rot severity in both Riesling & P. noir

Page 53: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Cluster Looseness and Disease Severity

P. noir, 2010

0

1

2

3

4

5C

heck

pre

-blo

om

leaf

GA

5 p

pm

pre

bl

GA

5 p

pm

GA

5 p

pm

2X

GA

10 p

pm

GA

20 p

pm

Pro

hex-C

a

low

Pro

hex-C

a

med

Pro

hex-C

a

hig

h

Sour rot Cluster Looseness

No Sig Dif among treatments -- Bloom timing for GA and Prohex Ca missed!!!

Page 54: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Sour Rot Severity, Riesling, 2010

0

2

4

6

8

10

12

Check Prebl leaf GA 5 ppm

Bl

GA 5 ppm

2X

GA 10

ppm Bl

GA 20

ppm

Prohex-Ca

low

Prodhex-

Ca med

Prohex-Ca

high

Sour rot Cluster looseness

No Sig Dif among treatments -- Bloom timing for GA and Prohex Ca missed!!!

Page 55: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Disease Severity and Cluster Looseness

Riesling, 2011

0

5

10

15

20

25

30

35

40

45

50

Chec

k

vera

ison

leaf

pea-

size

leaf

Pre

bl lea

f

GA 5

ppm

GA 5

ppm

2X

GA 1

0 pp

m

GA 2

0 pp

m

Pro

hex-C

a low

Pro

hex-C

a m

ed

Pro

hex-C

a high

Dis

ease S

everi

ty (

%)

0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

Clu

ste

r L

oo

sen

ess (

1-6

)

Sour rot Botrytis Cluster Looseness

Page 56: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Disease Severity and Cluster Looseness

Pinot noir, September, 2011

0

1

2

3

4

5

6

7

8

9

Untre

ated

peas

ize

leaf

preb

loom

leaf

GA 5

ppm

GA 5

ppm

2X

GA 1

0 pp

m

GA 2

0 pp

m

Pro

hex-C

a low

Pro

hex-C

a m

ed

Pro

hex-C

a high

Sour rot Botrytis Cluster looseness

No significant difference btw treatments & peasize leaf pulling

Page 57: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Disease severity October 2011

0

5

10

15

20

25

30

35

peasize leaf prebloom leaf Prohex-Ca med Prohex-Ca high GA 20 ppm

Sour rot Botrytis

Page 58: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Return Fruitfulness Riesling

2009/10 2010/11

Untreated 88 96

prebloom leaf 88 93

GA 5 ppm prebl 90 94

GA 5 ppm bl 92 97

GA 5 ppm 2X 86 96

GA 10 ppm bl 92 94

Prohex-Ca low 88 94

Prohex-Ca med 86 95

Prohex-Ca high 89 97

Page 59: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 60: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 61: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock
Page 62: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Reduce Mechanical Injury

• Suggestions for Cherry Cracking

– Osmoticum sprays

• Mineral salts (CaCl2) applied prior to or during rain

• Reduce absorption of water across skin

– Calcium

• Strengthen cell walls?

• Timing between fruit set and veraison

– Surfactants

• Raingard? (non-ionic surfactant)

• Desikote (new formulation of VaporGuard)

Page 63: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Reduce Mechanical Injury

• Treatments

• CaCl2

– Stopit (12% CaCl2 w/v)

• 1.64 gal/100 gal (US) biweekly (peasize berry + veraison)

• 0.87 gal/100 gal (US) weekly starting at peasize berry

• Leaf removal

– Peasize berry vs veraison

• Raingard

• Desikote

Page 64: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Treatments to Reduce Berry Injury

P. noir, 2011

0

2

4

6

8

10

12

Untreated peasize leaf 2X CaCl2 4X CaCl2 RainGard

Sour rot Botrytis

Page 65: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Treatments to Reduce Berry Injury

Riesling, 2011

0

10

20

30

40

50

Check veraison leaf pea-size leaf 2X CaCl2 4X CaCl2 Raingard Desikote

Sour rot Botrytis

Page 66: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Large Plot Calcium and Leaf Removal, P. noir, 2011

0

2

4

6

8

10

12

14

16

0Ca 2XCa 4XCa Pea-size Veraison

Sour rot Severity Sour rot 10%+ Bot rot Severity Bot rot 25%+

**

**

** ** **

**

** **

Page 67: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Sour Rot Management

• Potassium Metabisulphite?

– Used as anti-oxidant and anti-microbial (vs

microbes) in vinification (40-60 g/tonne)

– Rengasamy & Poole (NZ):

• 5 kg per 1000 L water

• Botrytis-infected berries dry out

– Wicks (Australia):

• 3-4 g/L KMS killed Botrytis spores & inhibited growth of

germ tubes

• If 4 g/L applied w/i 48 hr of infection, inhibits sporulation

from infected berries

• Little effect on sporulation after that

Page 68: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Sour Rot Management

• Potassium Metabisulphite (KMS)

– Concerns:

• Does it work?

– Rate? Timing?

• How does it work? (anti-oxidant/anti-microbial/both?)

• Excess sulphites & SO2 in wine?

Page 69: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Post-Veraison Treatments

• Treatments – KMS 2.5, 5, 10 kg/1000 L

– Milstop (K bicarb)

– KMS 5 kg + Milstop (K bicarb)

– Oxidate

– Blight Ban 506 (Pseudomonas fluorescens)

– Actinovate (Streptomyces lydicus)

– Agress (oxysilver nitrate)

– Vermicompost (not shown)

• 600 L/ha in fruiting zone @ – 50-75% veraison

– + 2 wk

– + 1 wk (Ries)

– + 1 wk (Ries)

– + 1 wk (1 wk pre-harvest)

– 3 d pre-harvest

– 1 d pre-harvest

Page 70: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Disease Severity with Post-Veraison Treatments

P. noir, 2011

0

1

2

3

4

5

6

7

8

9U

ntr

eate

d

peasiz

e leaf

KM

S 2

.5 k

g

KM

S 5

kg

KM

S 1

0 k

g

Kbic

arb

KM

S +

Kbic

arb

Agre

ss

Rain

Gard

Sour rot Botrytis

No significant difference btw treatments & peasize leaf pulling check

Page 71: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Disease Severity with Post-Veraison Treatments

Riesling, 2011

0

5

10

15

20

25

30

35

40

45

50

Chec

k

vera

ison

leaf

pea-

size

leaf

Actinov

ate

San

idate

Blig

ht B

an

Agr

ess 0.5

x

Agr

ess1x

Agr

ess 2x

KM

S 2.5

kg

KM

S 5 kg

KM

S 10

kg

Mils

top

KM

S 5 +

Mils

top

Sour rot Botrytis

Page 72: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Disease Severity with Post-Veraison Treatments

Riesling, 2011

0

5

10

15

20

25

30

35

40

45

50

Check veraison

leaf

pea-size

leaf

KMS 5 kg KMS 2 w k

post-ver

KMS 3 w k

post-ver

KMS 1 w k

pre-harv

KMS 3 d

pre-harv

KMS 1 & 3

d pre-harv

KMS 1 d

pre-harv

Sour rot Botrytis

Page 73: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

All Treatments

Riesling, 2011

Page 74: What we’ve learned about sour rot: An update on research · •Vincor Canada • Schenck Greenhouses and Farms Ltd. Acknowledgements •Dr. Debra Inglis (Co-researcher, CCOVI, Brock

Future Research

• Effects of temperature & wetness duration on infection

• Effect of soil moisture/soil type/vine vigour on disease development

• Changes in berry between 13 and 15 Brix that relate to susceptibility

• Screen other potential control agents

• Repeat prebloom leaf removal, GA and Prohex-Ca on same plots

• Effect of temperature on KMS activity

• Interactions between causal organisms

• Progression of berry microflora pea-size to harvest