Welcome to

Poultry Tech Summit 2019

Dr. Caitlin CooperCSIRO Australian Animal Health LaboratoryGenome Engineering Team

7 billion

Need a fast, accurate, cost effective solution

Males don’t lay eggs …

$500Msavings

Labor intensiveOnly 95% accurate

Global stock value

$6B/pa

7B males incubated,

hatched then culled

Wastefulethical

problem

The AdaptivEggs advantage

Selectively incubate and hatch females

Selectively incubating and hatching female chicks is a priority for the industry

Detect and remove males

prior to incubation

Point of lay High through-put

High accuracyNon-invasive

Applications for male embryos in biomedicine

Male egg removed at point of lay

ZFP Z

Female incubated and

hatched

Marked male production generation

Z W

ZFP W Z Z

Identified as male or female

Male eggs removed and used for other purposes

Female eggs incubated to hatch layer hens

Up to 6¢ savings per egg

Hatchery point of lay detection

Labor, Incubation, Waste

ZFP Z Z W

World first POL detection

No target detected Target detected

Z W ZFP Z

Great Grand Parent Stock

Grandparent Stock

Parent Stock

Commercial layers

Processing/Retailers

Consumers

Pedigree selection

Genetic improvement

Global genetics companies

Breeders

Hatcheries

Farmers

Pure Lines AdaptivEggs marker integration

AdaptivEggs marker enabled point of lay sex sorting

Industry integration 24 - 36 months

6 months

6 months

6 months

Grand Parent Lines

Parent Lines

Commercial chickens

Great Grand Parent Lines

ZFP ZFP

ZFP WZFP ZFP

ZFP W

Z W

Z Z

Z W ZFP Z

Detected and removed at point of lay

A line B line C line D line

ZFP Marked Z chromosomeZ Unmarked Z chromosomeW Unmarked W chromosomeZFP ZFP /Z Z = Male ZFP W / Z W = Female

Marker passed through breeding

• Many vaccines are produced in embryonated eggs

• These eggs are very similar to embryonated eggs produced in the layer industry

• Multiple ways to improve embryonated eggs so they are tailor-made for vaccine production

• New use for sorted male embryos

Improved eggs for vaccine production

Genetically engineered line of chickens producing eggs for improved vaccine production

Wildtype

Modified

Expression of a novel catalysis enzyme that improves epitopes for vaccine growth

Chicken epitope

Novel epitope

Chicken epitope

Novel epitope

Thank you