Welcome to
Poultry Tech Summit 2019
Dr. Caitlin CooperCSIRO Australian Animal Health LaboratoryGenome Engineering Team
7 billion
Need a fast, accurate, cost effective solution
Males don’t lay eggs …
$500Msavings
Labor intensiveOnly 95% accurate
Global stock value
$6B/pa
7B males incubated,
hatched then culled
Wastefulethical
problem
The AdaptivEggs advantage
Selectively incubate and hatch females
Selectively incubating and hatching female chicks is a priority for the industry
Detect and remove males
prior to incubation
Point of lay High through-put
High accuracyNon-invasive
Applications for male embryos in biomedicine
Male egg removed at point of lay
ZFP Z
Female incubated and
hatched
Marked male production generation
Z W
ZFP W Z Z
Identified as male or female
Male eggs removed and used for other purposes
Female eggs incubated to hatch layer hens
Up to 6¢ savings per egg
Hatchery point of lay detection
Labor, Incubation, Waste
ZFP Z Z W
World first POL detection
No target detected Target detected
Z W ZFP Z
Great Grand Parent Stock
Grandparent Stock
Parent Stock
Commercial layers
Processing/Retailers
Consumers
Pedigree selection
Genetic improvement
Global genetics companies
Breeders
Hatcheries
Farmers
Pure Lines AdaptivEggs marker integration
AdaptivEggs marker enabled point of lay sex sorting
Industry integration 24 - 36 months
6 months
6 months
6 months
Grand Parent Lines
Parent Lines
Commercial chickens
Great Grand Parent Lines
ZFP ZFP
ZFP WZFP ZFP
ZFP W
Z W
Z Z
Z W ZFP Z
Detected and removed at point of lay
A line B line C line D line
ZFP Marked Z chromosomeZ Unmarked Z chromosomeW Unmarked W chromosomeZFP ZFP /Z Z = Male ZFP W / Z W = Female
Marker passed through breeding
• Many vaccines are produced in embryonated eggs
• These eggs are very similar to embryonated eggs produced in the layer industry
• Multiple ways to improve embryonated eggs so they are tailor-made for vaccine production
• New use for sorted male embryos
Improved eggs for vaccine production
Genetically engineered line of chickens producing eggs for improved vaccine production
Wildtype
Modified
Expression of a novel catalysis enzyme that improves epitopes for vaccine growth
Chicken epitope
Novel epitope
Chicken epitope
Novel epitope
Thank you