warnings and precautions - grupo sym · df6 op dataplot group fields,4-24 retic histogram...

PN 4277303D (September 2004)

Beckman Coulter, Inc.Fullerton, CA 92835

COULTER® LH Series WorkStation

Host Transmission Specification

� � � � � � � � � � � � � � � ��

GEN•S

READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE ATTEMPTING TO OPERATE INSTRUMENT. DO NOT ATTEMPT TO PERFORM ANY PROCEDURE BEFORE CAREFULLY READING ALL INSTRUCTIONS. ALWAYS FOLLOW PRODUCT LABELING AND MANUFACTURER’S RECOMMENDATIONS. IF IN DOUBT AS TO HOW TO PROCEED IN ANY SITUATION, CONTACT YOUR BECKMAN COULTER REPRESENTATIVE.

BECKMAN COULTER, INC. URGES ITS CUSTOMERS TO COMPLY WITH ALL NATIONAL HEALTH AND SAFETY STANDARDS SUCH AS THE USE OF BARRIER PROTECTION. THIS MAY INCLUDE, BUT IT IS NOT LIMITED TO, PROTECTIVE EYEWEAR, GLOVES, AND SUITABLE LABORATORY ATTIRE WHEN OPERATING OR MAINTAINING THIS OR ANY OTHER AUTOMATED LABORATORY ANALYZER.

HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS

WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:

WARNING - Can cause injury.CAUTION - Can cause damage to the instrument.

IMPORTANT - Can cause misleading results.

WARNING Risk of operator injury if:

r All doors, covers and panels are not closed and secured in place prior to and during instrument operation. r The integrity of safety interlocks and sensors is compromised. r Instrument alarms and error messages are not acknowledged and acted upon. r You contact moving parts. r You mishandle broken parts. r Doors, covers and panels are not opened, closed, removed and/or replaced with care. r Improper tools are used for troubleshooting.

To avoid injury:

r Keep doors, covers and panels closed and secured in place while the instrument is in use. r Take full advantage of the safety features of the instrument. Do not defeat safety interlocks and sensors. r Acknowledge and act upon instrument alarms and error messages. r Keep away from moving parts. r Report any broken parts to your Beckman Coulter Representative. r Open/remove and close/replace doors, covers and panels with care. r Use the proper tools when troubleshooting.

CAUTION System integrity might be compromised and operational failures might occur if:

r This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals. r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s

computer with software authorized by Beckman Coulter. r You install software that is not an original copyrighted version. Only use software that is an original copyrighted

version to prevent virus contamination.

IMPORTANT If you purchased this product from anyone other than Beckman Coulter or an authorized Beckman Coulter distributor, and, if it is not presently under a Beckman Coulter service maintenance agreement, Beckman Coulter cannot guarantee that the product is fitted with the most current mandatory engineering revisions or that you will receive the most current information bulletins concerning the product. If you purchased this product from a third party and would like further information concerning this topic, call your Beckman Coulter Representative.

WARNINGS AND PRECAUTIONS

REVISION STATUS

Initial Issue, 7/01Software Version 1A.

Revision B, 4/02Software Version 2A. Derived from internal Part Number 0302000G.Updated Section 4.3, Dilution Factor field comments.Removed all references to “+ Subset” globally.Added WBC Threshold Full Histogram Group Fields.Added new Section 3.18, Collate the ToDo List.Updated General Information Group Fields from 29 groups to 24 groups.Added notes to Tables 4-3 and 4-15, respectively.Updated the following tables: 4-1; 4-3; 4-6; 4-8; 4-12; 4-13; 4-21; 4-30; 4-31; 4-33; 4-39; 4-41; 4-46; 4-48; 4-52; 5-1; 5-2; 5-4.Removed NRBC note from Tables 4-24 and 4-46.Added “Note: Parameter selection does not affect control transmissions.” to Tables 4-43; 4-44; 4-46; 4-48; and 4-50.Updated Control Information Group 34, removed reference WBC Count Field.Updated Section 6.2, added SOH to table.Updated Section 8.1.

Revision C, 9/03Software Version 2B. Derived from internal Part Number 0302000K.Updated the following tables: 4-23; 4-24; 4-43; 4-4; 4-46; 4-48; 4-50.Added new section 3.19.

Revision D, 9/04LH 700 Software Version 2B and LH 500 Software Version 2A. Derived from internal Part Number 0302000N.Updated the following tables: 4.12; 4.20; 4.22; 4.25; 4.28; 4.30; 4.37; 5.1.

iiiPN 4277303D

This document applies to the latest software listed and higher versions. When a subsequent software version changes the information in this document, a new issue will be released.

REVISION STATUS

PN 4277303D iv

CONTENTS

REVISION STATUS, iii

1 INTRODUCTION, 1-1

1.1 PURPOSE, 1-1

2 HARDWARE INTERFACE, 2-1

2.1 HARDWARE HANDSHAKE, 2-1

3 COMMUNICATION PARAMETERS, 3-1

3.1 COMMUNICATION PARAMETERS, 3-1

3.2 COMMUNICATION PROTOCOLS, 3-1Low Level Protocol, 3-1High Level Protocol, 3-1

3.3 TIME-OUT, 3-2

3.4 BAUD RATE, 3-2

3.5 DATA BITS, 3-2

3.6 PARITY, 3-2

3.7 STOP BITS, 3-2

3.8 HANDSHAKE, 3-2

3.9 BLOCK SIZE, 3-2

3.10 GRAPHICS, 3-2

3.11 COMPATIBILITY, 3-3

3.12 SPOOLER, 3-3

3.13 CLEARING THE SPOOLER, 3-3

3.14 REPLACE NULL BY SP, 3-3

3.15 DISABLING HOST COMMUNICATIONS, 3-3

3.16 ENABLE VALIDATION CODES, 3-3

3.17 REPLACE DLE E WITH DLE C, 3-4

3.18 COLLATE THE TODO LIST, 3-4

3.19 REPLACE V AND V FLAGS WITH R (LH 500 ONLY), 3-4

4 LH SERIES WORKSTATION TO HOST COMMUNICATIONS, 4-1

4.1 DATA-LINK, 4-1

PN 4277303D v

CONTENTS

Full Software Handshake, 4-1Software Handshake Action, 4-1No Handshake, 4-2Data Block Structure, 4-2Group Definition Locator Table, 4-3

4.2 1G.1 + RETICS FORMAT, 4-4Message Structure, 4-4

Preamble, 4-4Group, 4-4Field Count, 4-4Fields, 4-5Postamble, 4-5General Information Group Fields, 4-6CBC Parameter Group Fields, 4-9DIFF Count Parameter Group Fields, 4-10DIFF Percent Parameter Group Fields, 4-12Comment Group Fields, 4-14Flag Group Fields, 4-14Demographics Group Fields, 4-18DF1 DataPlot Group Fields, 4-20DF2 DataPlot Group Fields, 4-20DIFF (VCS) Histogram Group Fields, 4-20RBC Histogram Group Fields, 4-21PLT Histogram Group Fields, 4-21RETIC Parameters Group Fields, 4-22DF5 LS DataPlot Group Fields, 4-24DF6 OP DataPlot Group Fields, 4-24RETIC Histogram Group, 4-25

4.3 LH 750 WORKSTATION FORMAT, 4-25Message Structure, 4-25Group Definition, 4-28

General Information Group Fields, 4-29CBC Parameters Group Fields, 4-33DIFF Count Parameters Group Fields, 4-35DIFF Percent Parameters Group Fields, 4-37Comment Group Fields, 4-38Definitive Flags Group Fields, 4-39Suspect Flags Group Fields, 4-40Conditional Flags Group Fields, 4-41Other Flags Group Fields, 4-43Demographics, 4-43RBC Histogram Group Fields, 4-47PLT Histogram Group Fields, 4-47RETIC Parameters Group Fields, 4-48WBC Threshold Group Fields, 4-50WBC Threshold Full Histogram Group Fields, 4-50DIFF Latex Parameters Group Fields, 4-50RETIC Latex Parameters Group Fields, 4-52

PN 4277303Dvi

CONTENTS

Control CBC Parameters Group Fields, 4-56Control DIFF Count Parameters Group Fields, 4-57Control DIFF Percent Parameters Group Fields, 4-58Control RETIC Parameters Group Fields, 4-59DIFF Graphics, 4-61Retic Graphics, 4-61

4.4 HOST PARSING OF TRANSMITTED DATA, 4-62

5 HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST), 5-1

5.1 DATA-LINK, 5-1Protocol, 5-1Data Block Structure, 5-4

5.2 PRESENTATION, 5-4Message Structure, 5-4

DLE Responses From The Workstation And Effects After A ToDo List Download, 5-5

5.3 MESSAGE DEFINITION, 5-7

5.4 DELETE MESSAGE, 5-14

6 ASCII Tables, 6-1

6.1 7-BIT ASCII CODES, 6-1

6.2 VALID HOST COMMUNICATIONS ASCII CODES, 6-1

7 CRC, 7-1

7.1 CRC ALGORITHM, 7-1

7.2 CRC EXAMPLE WRITTEN IN ASM86, 7-2

7.3 CRC EXAMPLE WRITTEN IN C, 7-3

8 GRAPHIC RECONSTRUCTION, 8-1

8.1 RBC, WBC THRESHOLD, AND PLT HISTOGRAMS, 8-1

8.2 LH 750 DATAPLOT FORMAT, 8-1Dataplots, 8-1

Dataplot Data Header, 8-2Dataplot Data Header Elements, 8-2Dataplot Data Block, 8-2Dataplot Data Block Header, 8-3Rendering Information Block, 8-3Rendering Information Block Header, 8-4Color Palette, 8-4Dithered Display Bitmap Library, 8-4

PN 4277303D vii

CONTENTS

Dataplot Header, 8-5

TRADEMARKS, 1

COULTER LH SERIES SYSTEM HARD-COPY DOCUMENTATION, 2

ILLUSTRATIONS8.1 Plotting RBC, WBC Threshold and PLT Histograms, 8-18.2 Dataplot Transmission, 8-28.3 Dataplot Data Header, 8-28.4 Dataplot Data Block Format, 8-28.5 Dataplot Data Block Header, 8-38.6 Rendering Information Block, 8-48.7 Rendering Information Block Header Format, 8-48.8 Palette Table Entry, 8-48.9 Bitmap Format, 8-58.10 Dataplot Header, 8-58.11 Dataplot Data Format, 8-6

PN 4277303Dviii

CONTENTS

TABLES3.1 Communication Parameters Available by Format, 3-13.2 Graphics Options, 3-34.1 Group Definition Locator, 4-34.2 Group Definition: 1G.1 + Retics, 4-64.3 General Information -- Group 1, 4-64.4 CBC Parameters -- Group 2, 4-94.5 Flag Data for CBC Parameter Group Fields, 4-104.6 DIFF Count Parameters -- Group 3, 4-104.7 Flag Data for Diff Count Parameter Group Fields, 4-124.8 DIFF Percent Parameters -- Group 4, 4-124.9 Flag Data for DIFF Percent Parameter Group Fields, 4-134.10 Comment -- Group 5, 4-144.11 Flag -- Group 6, 4-144.12 LH 750 Workstation/STKS 1G.1 + Retics Messages, 4-154.13 Demographics -- Group 7, 4-184.14 RBC Histogram -- Group 11, 4-214.15 PLT Histogram -- Group 12, 4-214.16 RETIC Parameters -- Group 13, 4-224.17 Flag Data for Retic Parameter Group Fields, 4-244.18 Transmission Tests and Test IDs by Test Type, 4-254.19 Transmitted Groups, 4-284.20 General Information -- Group 1, 4-294.21 Possible Values for Sample Status, 4-334.22 CBC Parameters -- Group 2, 4-334.23 Flag Data for CBC Parameter Group Fields, 4-344.24 DIFF Count Parameters -- Group 3, 4-354.25 Flag Data for DIFF Count Parameter Group Fields, 4-364.26 DIFF Percent Parameters -- Group 4, 4-374.27 Flag Data for DIFF Percent Parameter Group Fields, 4-384.28 Comment -- Group 5, 4-384.29 Definitive Flags -- Group 6, 4-394.30 Suspect Flags -- Group 7, 4-404.31 Conditional Flags -- Group 8, 4-414.32 Other Flags -- Group 9, 4-434.33 Demographics -- Group 10, 4-434.34 RBC Histogram -- Group 14, 4-474.35 PLT Histogram -- Group 15, 4-474.36 RETIC Parameters -- Group 16, 4-484.37 Flag Data for Retic Parameter Group Fields, 4-494.38 WBC Threshold Monitor Group 26, 4-504.39 DIFF Latex Parameters -- Group 31, 4-504.40 Flag Data for DIFF Latex Parameters Group Fields, 4-514.41 RETIC Latex Parameters -- Group 32, 4-524.42 Flag Data for RETIC Latex Parameters Group Fields, 4-534.43 Control Information -- Group 34, 4-534.44 Control CBC Parameters -- Group 35, 4-564.45 Flag Data for Control CBC Parameters Group Fields, 4-574.46 Control DIFF Count Parameters -- Group 36, 4-57

PN 4277303D ix

CONTENTS

4.47 Flag Data for DIFF Count Parameter Group Fields, 4-584.48 Control DIFF Percent Parameters -- Group 37, 4-584.49 Flag Data for Diff Percent Parameter Group Fields, 4-594.50 Control RETIC Parameters -- Group 38, 4-594.51 Flag Data for Control RETIC Parameter Group Fields, 4-614.52 DIFF DataPlot -- Group 50, 4-614.53 Retic DataPlot -- Group 51, 4-615.1 Message Definition Table, 5-75.3 Possible Combinations of Sample Requests with Multiple Test Requests, 5-135.2 Possible Values and Examples of Test Requests, 5-135.4 Patient ID Deletions, 5-16

PN 4277303Dx

1
1INTRODUCTION
1.1 PURPOSEThis document is the specification for communication between the LH Series Workstation and a host computer. The functionality will remain backward compatible. This document is intended for use by software engineers needing to either maintain or modify the operation of the LH Series Workstation Host Communications.

This document describes the transfer of data from the LH Series Workstation to an LIS/HIS computer in the following formats:

r STKS 1G.1 + Retics

r LH 750 Workstation: host communication protocol which supports transmission of CBC, DIFF and Reticulocytes parameters for samples and control runs as well as collated sample runs. This format is an extension of the STKS 2A and 3A formats.

The LH 750 “STKS 1G.1 + Retics” format is provided for use with existing STKS™ and MAXM™ interfaces. Due to the LH Series Workstation’s new features and enhancements, STKS and MAXM instrument formats are not completely supported (see details in following sections).

PN 4277303D 1-1

INTRODUCTIONPURPOSE

PN 4277303D1-2

2
2HARDWARE INTERFACE
The system is equipped with a Host connector on the rear of the LH SeriesWorkstation that lets the system interface with a host computer via a Standard EIA-type 25-pin connector and uses EIA Standard RS-232-C signals. The LH 500 is equipped with a 9 pin to 25 pin adapter allowing the the system to interface with a host computer. This is a Data Terminal Equipment (DTE) configuration.

The LH SeriesWorkstation/Host communications uses only the following signals:

The LH SeriesWorkstation is the DTE, by RS-232 standards, which explains the RTS/DTR pin-outs. Whether the HOST is a DTE or DCE will determine its use of CTS/DSR or RTS/DTR. A DTE HOST requires the use of a NULL MODEM Cable.

Note: Pin number 5 CTS must be active for LH Series Workstation to send any transmissions. If the host computer does not support the above hardware handshake lines, it will be necessary to connect pin 4 to pin 5 and to connect pin 20 to pin 6.

2.1 HARDWARE HANDSHAKEDuring a transmission, the host logically raises CTS to allow the LH Series Workstation to send data and logically lowers CTS to prevent the LH Series Workstation from sending data.

Note: If CTS is lowered to hold off the LH Series Workstation host transmission, it must be raised again within the Workstation time-out period. If CTS is not raised within the time-out period, the LH Series Workstation transmission to the host will time-out and reset, ready for the next transmission.

P3 Pin No. Signal Name Flow Direction

2 Transmit Data From LH Series Workstation

3 Received Data To LH Series Workstation

4 Ready To Send (RTS) From LH Series Workstation

5 Clear To Send (CTS) To LH Series Workstation

6 Data Set Ready (DSR) To LH Series Workstation

7 Signal Ground Reference

8 Carrier Detect Reference

20 Data Terminal Ready (DTR) From LH Series Workstation

22 Ring Indicator Reference

PN 4277303D 2-1

HARDWARE INTERFACEHARDWARE HANDSHAKE

Diagram of Hardware Handshake Mode

LH Series Workstation (SENDER) Host (RECEIVER)

<-- CTS on (send me data)

send data ----->

send data ----->

send data ----->

<-- CTS off (stop sending data)

wait

• process received data

• •

• •

• •

•

<-- CTS on (send more data)

send data ----->

send data ----->

- etc.-

PN 4277303D2-2

3
3COMMUNICATION PARAMETERS
3.1 COMMUNICATION PARAMETERSThe LH SeriesWorkstation allows the user to configure the following communication parameters on the Host Computer Definition screen. These parameters include communication modes, as well as parameters enabling the transmission of graphic data.

3.2 COMMUNICATION PROTOCOLSThe transmission specification consists of data-link (low level) and presentation (high level) protocols. Data-link protocol provides the means of transmitting data without any regard to actual information. Presentation protocol describes the actual information and its format.

Low Level ProtocolThe Host Low Level Communication Protocol will be supported by all formats.

High Level ProtocolThe High Level Communication Protocols currently supported are the “STKS 1G.1 + Retics” and “LH 750 Workstation”. The High Level Communication Protocol is selected using the compatibility field.

Note: To satisfy the host communication requirements, the receiver MUST parse for all of the data.

DO NOT ASSUME FIXED OFFSETS

Table 3.1 lists the communication parameters available by format.

Table 3.1 Communication Parameters Available by Format

Communication Parameter 1G.1 + Retics LH 750 WorkstationTime-out X XBaud Rate X XData Bits X XParity X XStop Bits X XHandshake X XBlock Size X XGraphics X XCompatibility X X

X in 1G.1 + Retics column = Supported by the LH 750 “STKS 1G.1 + Retics ” format.X in LH 750 Workstation column = Supported by the LH 750 Workstation format.

PN 4277303D 3-1

COMMUNICATION PARAMETERSTIME-OUT

3.3 TIME-OUTThe time-out value determines the amount of time the LH SeriesWorkstation waits for a response from the host before retrying to send data to the host. The LH SeriesWorkstation continues to transmit at the time-out intervals until the host successfully receives the data.

The time-out values are 30, 60, and 90 seconds. The default value is 90 seconds.

3.4 BAUD RATEThe following baud rates are supported: 2400, 4800, 9600, 19200 and 38400.

Note: The minimum recommended baud rate is 2400 for non-graphic transmissions and 9600 Baud for graphic transmissions.

3.5 DATA BITSHost Communication can be configured for 7 or 8 bit mode.

3.6 PARITYOdd, Even, and Off (None) parity modes are supported. Odd parity is the default value.

3.7 STOP BITSNone, 1, or 2 Stop Bit modes are supported. The default value is 2.

3.8 HANDSHAKEThis option determines whether or not the LH SeriesWorkstation looks for a handshake from the host computer. See Heading 4.1, Data-Link, for additional information.

3.9 BLOCK SIZEThe LH SeriesWorkstation/Host Communications support block sizes of 128 and 256 bytes. The default value is 256 bytes.

3.10 GRAPHICSThe LH SeriesWorkstation allows DIFF and Retic graphic transmission if the compatibility format selected is the LH SeriesWorkstation format.

Table 3.2 lists the graphic items that can be enabled or disabled for the corresponding test based on the current compatibility format and report profile selected.

PN 4277303D3-2

COMMUNICATION PARAMETERSCOMPATIBILITY 3

3.11 COMPATIBILITYThis option determines which transmission format is used by the LH SeriesWorkstation to send results to an LIS/HIS computer. The Host High Level Communication Protocols supported are: “STKS 1G.1 + Retics” and “LH 750 Workstation”.

3.12 SPOOLERTransmissions to the Host are spooled before sending. When a transmission is sent to the Host, it will be kept on the Spooler and resent until the Host acknowledges that the transmission was successfully received. If the Host is offline when Transmit To Host is enabled, all transmissions intended for the Host will be kept on the Spooler until the Host comes online or the Host Spooler is cleared.

3.13 CLEARING THE SPOOLERSelect the Clear button in the LIS folder on the System Run Configuration screen to clear all spooled transmissions intended for the Host. It should be remembered that new transmissions to the Host will still be subsequently spooled. See Section 3.15 to disable host communications and hence the host spooler.

3.14 REPLACE NULL BY SPThis is no longer an option because NULL characters are not included in the transmission buffer.

3.15 DISABLING HOST COMMUNICATIONSSelect the Disable button in the LIS folder on the System Run Configuration screen to disable all auto transmissions from the LH SeriesWorkstation to the Host. This will not affect manual transmissions or transmissions from the Host to the LH SeriesWorkstation.

3.16 ENABLE VALIDATION CODESThis checkbox enables the codes used to identify the validation status of individual test requests on a sample as detailed in Conditional Flags Group Fields under AutoValidation in Chapter 4. If “Enable Validation Codes” in System Setup - [Communications] is checked (default), the individual test group validation status will be transmitted. Applied only to LH 750 format.

Table 3.2 Graphics Options

Test Graphics 1G.1 + Retics LH 750 WorkstationCBC RBC Histogram X X

PLT Histogram X XWBC Threshold N/A X

DIFF DataPlot N/A XRETICS DataPlot N/A XSubset DataPlot N/A X

X in 1G.1 + Retics column = Supported by the LH 750 STKS 1G.1 + Retics ” format.X in LH 750 Workstation column = Supported by the LH 750 Workstation format.

PN 4277303D 3-3

COMMUNICATION PARAMETERSREPLACE DLE E WITH DLE C

3.17 REPLACE DLE E WITH DLE CSelect this checkbox to replace DLE E with DLE C in the standard data set transmitted to your information system.

3.18 COLLATE THE TODO LISTThe ToDo List generation is derived from task pre-assignment, by determining what sets of data are to be collated into a final report, test panels are pre-assigned either manually or by LIS download.

3.19 REPLACE V AND V FLAGS WITH R (LH 500 ONLY)If transmitting in the protocol “STKS 1G.1 + Retics” all transmissions of V and v flags will be sent as R. If transmitting in the “LH 750 Workstation” protocol, transmissions of V and v flags will be sent as R only if the “Replace V and v flags with an R” option is enabled, otherwise V and v flags will be sent as V and v.

PN 4277303D3-4

4
4LH SERIES WORKSTATION TO HOST COMMUNICATIONS
4.1 DATA-LINKA sample transmission to the Host consists of one or more framed data blocks. See Data Block Structure below. All transmitted data bytes are ASCII characters. All numeric values are ASCII representations of hexadecimal numbers. Hence, for example, the number FF (hex) is represented by the two ASCII bytes "FF" (46H, 46H). The first two bytes of the transmission are the number of blocks to be sent, in ASCII, followed by transmission of data blocks (see format below).

The final block will be padded with ASCII spaces (20H) to fill a whole block.

The LH Series Workstation supports “full software handshake” and “no software handshake” protocols.

Full Software HandshakeThe LH Series Workstation sends the following control characters plus data and expects the indicated host response:

Up to 255 blocks of data can be sent. The actual number of blocks sent is specified by the two-byte ASCII Block Count.

Software Handshake ActionIf the host NAKs a data block, the block is retransmitted. It is up to the host to determine how many times it retries receiving a NAKed block before aborting. Typically, the Host will NAK a datablock if the current block number is incorrect(e.g. out of sequence), the Host’s calculated CRC does not match the transmitted block’s CRC or block framing is not recognized as correct.

A SYN sent by the Host after a block has been transmitted by the LH Series Workstation forces the Workstation to retransmit all data starting with the first block. This does not include the

LH Series Workstation (SENDER) HOST (RECEIVER)

SYN (ready)------>

<-------SYN (go ahead)

Block Count------>

<-------ACK (ready to receive)

or NAK (receiver abort)

send data blocks

Block ------>

for each block••••

<-------ACK (block received ok) or

NAK (retransmit this block) or

SYN (retransmit all blocks)

SYN (all done)------>

<-------ACK (transmission accepted)

PN 4277303D 4-1

LH SERIES WORKSTATION TO HOST COMMUNICATIONSDATA-LINK

initial SYN and block count. It is up to the Host to determine under what conditions to transmit a SYN.

The LH Series Workstation continues to send the same sample transmission until the Host accepts it entirely (ACKs each block). To abort a transmission, the host must ACK each block of the transmission and discard it locally.

No HandshakeIf “Handshake” option is disabled, the protocol ignores all hardware and software host hanshake responses. In addition, in the No Handshake mode, the LH SeriesWorkstation does not send a SYN prior to transmitting the data. CTS is always used to indicate that a Host is MS and turned on.

Data Block Structure

Each data block is framed with a Start Of Text(STX = 02H) and an End Of Text(ETX =03H) control character.

The next two bytes after STX have the Current Block Sequence Number(BLK NBR) in ASCII starting with the number 1(MS byte = 30H, LS byte = 31H, to give ‘01’) for the first block. The block number of the last block must equal the Block Count sent at the beginning of the transmission.

Every data block will have either 128 or 256 bytes (whichever is chosen). If there is insufficient data to fill a block, it will be padded with space characters (last block transmitted).

The CRC for the block’s data is calculated by the workstation and sent as the last four bytes of the block before the ETX in the ASCII format shown above. The Most Significant Byte (MSB) is generated as a Most Significant and Least Significant ASCII character (MS and LS CHAR). The same is true for the Least Significant Byte (LSB).

Byte # # of bytes

1 STX 1 byte

2 BLK NBR MS CHAR 1 byte

3 BLK NBR LS CHAR 1 byte

4 DATA BYTES•

•

•

256 (128) C bytes R

C

259 (131)

260 (132) CRC MSB MS CHAR 1 byte

261 (133) CRC MSB LS CHAR 1 byte

262 (134) CRC LSB MS CHAR 1 byte

263 (135) CRC LSB LS CHAR 1 byte

264 (136) ETX 1 byte

PN 4277303D4-2

LH SERIES WORKSTATION TO HOST COMMUNICATIONSDATA-LINK 4

The algorithm used to calculate the CRC for each block is a modified CCITT CRC16. CRC is only calculated on the data bytes.

Chapter 7 details the CRC algorithm and includes application notes for implementing the algorithm in both C and assembly language.

Group Definition Locator TableTable 4.1 lists the group names for each format.

Table 4.1 Group Definition Locator

Group Name Group Number1G.1 + Retics,Heading 4.2

Group Number

LH 750 Workstation,Heading 4.3

General Information 1 X 1 XCBC Parameters 2 X 2 XDIFF Count Parameters 3 X 3 XDIFF Percent Parameters 4 X 4 XComment 5 X 5 XDefinitive Flags 6 XSuspect Flags 7 XConditional Flags 8 XOther Flags 9 XFlags 6 XDemographics 7 X 10 XDF1 DataPlot 8 X 11DF2 DataPlot 9 X 12DIFF (VCS) Histogram 10 X 13RBC Histogram 11 X 14 XPLT Histogram 12 X 15 XRETICS Parameters 13 X 16 XDF5 LS DataPlot 14 X 17DF6 OP DataPlot 15 X 18RETICS Histogram 16 X 19WBC Threshold 26 XWBC Threshold Full Histogram 27 XDIFF Latex Parameters 31 XRETIC Latex Parameters 32 XControl Information 34 XControl CBC Parameters 35 XControl DIFF Count Parameters 36 XControl DIFF Percent Parameters 37 XControl RETICS Parameters 38 XDIFF graphics 50 XRetic graphics 51 X

X in 1G.1 + Retics column = Described under Heading 4.2, STKS 1G.1 + Retics Format.X in LH 750 Workstation column = Described under Heading 4.3, LH 750 Workstation Format.

PN 4277303D 4-3

LH SERIES WORKSTATION TO HOST COMMUNICATIONS1G.1 + RETICS FORMAT

4.2 1G.1 + RETICS FORMAT

Message StructureThe Presentation deals with the high level format of the message. The data bytes of the transmission blocks, when collected together, exhibit the following high level format.

PreambleThe preamble marks the beginning of a message.

Group

The DC1 (11H) character marks the beginning of a Group. There is no relationship between the order in which the groups are defined in the following sections and the order in which they appear after the preamble. Groups will never be omitted.

Each Group contains a DC1 (11H) character, marking the beginning of the section, a two character field count and a variable number of fields each ending with a CR (0DH) and an LF (0AH).

Note: When there are no data for a Group, the Group will appear as follows:

Field CountEach Group has a Field Count which identifies the number of fields contained within the Group. Valid field counts can be from 0-255. The field count uses two characters to give the ASCII representation of the hexadecimal value.

The field count will reflect the total number of fields transmitted within the Group. The field count will always be the sum of CR/LF pairs within the Group minus one.

PREAMBLE GROUP x1 • • • GROUP xn POSTAMBLE

CR

LF

CR

LF

CR

LF

CR

LF

CR

LF

CR

LF

-

-

-

-

-

-

-

-

-

-

-

-

-

-

CR

LF

DC1

FIELD

COUNT

CR

LF

FIELD

1

CR

LF

• • • • FIELD

N

CR

LF

DC1

0

0

CR

LF

MS

CHAR

LS

CHAR

Two-byte ASCII representation of Hex value between 00 and FF (0 to 255)

PN 4277303D4-4

LH SERIES WORKSTATION TO HOST COMMUNICATIONS1G.1 + RETICS FORMAT 4

Fields Each Group is made up of variable length fields which may or may not be padded with SP (20H) characters. A field may have a variable length tag preceding the data separated with one or more SP (20H) characters. The data portion of a field will only contain ASCII characters in the range of 20H to 7EH inclusive. Chapter 6 tabulates the valid ASCII characters. Fields are separated by a CR (0DH) and an LF (0AH) character.

Note: A field's position within a Group must not be used to identify it. Fields within a Group may appear in any order or may be omitted. The CR/LF should be used to find a field and the Tag used to identify it.

The syntax for the Data Format of a field is:

Postamble

The postamble marks the end of the current message. There are 16 groups defined in the current implementation of the “STKS 1G.1 + Retics” format. Future revisions may include additional groups. Any future additions will not disrupt the numbering of the groups as presently defined.

A - Alpha characters (a..z and A..Z and space) or (61H..7AH and 41H..5AH and 20H)

N - Numeric characters (0..9 and + - .) or (30H..39H and 2BH 2DH 2EH)

X - Printable characters (20H..7EH)

Sep - Separator one or more SP characters

SP Space (ASCII 20H). Please note that all field transmissions will include a SP between the tag and data format (this will be implied in the following sections), as illustrated below:

Tag Sep Data Format

T I M E N N : N N : N N

DC1

CR

LF

CR

LF

-

-

-

-

-

-

-

-

-

-

-

-

-

-

CR

LF

PN 4277303D 4-5


Group Definition

Table 4.2 lists the groups supported by the 1G.1 + Retics format.

General Information Group FieldsTable 4.3 identifies the field names, test types, and applicable format of General Information Group transmissions.

Table 4.2 Group Definition: 1G.1 + Retics

GroupNumber Group Name

1G.1 +Retics +Subsets

1 General Information X

2 CBC Parameters X

3 DIFF Count Parameters X

4 DIFF Percent Parameters X

5 Comments X

6 Flags X

7 Demographics X

8 DF1 DataPlot *

9 DF2 DataPlot *

10 DIFF (VCS) Histogram *

11 RBC Histogram X

12 PLT Histogram X

13 Retic Parameter X

14 DF5 DataPlot *

15 DF6 DataPlot *

16 Retic Histogram *

X = Supported by the LH 750, STKS 1G.1 + Retics ” format.* = Not supported the LH 750 “STKS 1G.1 + Retics ” format. The Group is transmitted with a

field count of 00.

Table 4.3 General Information -- Group 1

Field Name Test Type 1G.1 + Retics

Run Date C, CD, CDR, CR, R X

Run Time C, CD, CDR, CR, R X

ID #1 C, CD, CDR, CR, R X

ID #2 C, CD, CDR, CR, R X

Cassette/Position C, CD, CDR, CR, R X

Sequence Number C, CD, CDR, CR, R X

1E Cassette/Position C, CD, CDR, CR, R X

ID #1 Status C, CD, CDR, CR, R X

PN 4277303D4-6


Run Date Field:

Run Time Field:

ID #1 Field:

ID #2 Field:

Cassette/Position Status C, CD, CDR, CR, R X

ToDo List Status C, CD, CDR, CR, R X

X = Supported by the LH 750, “STKS 1G.1 + Retics” format.

Tag Data Format Data Length Comments

D A T E N N / N N / N N 9 bytes Date output is month/day/year, regardless of the system date configuration.


T I M E N N : N N : N N 12 bytes Time output is on 24 hours, regardless of the system time configuration. Time ouput is hours:minutes:seconds..


I D 1 X X X • • • X X X X X 16 bytes SAMPLE ID


I D 2 X X X • • • X X X X X 16 bytes Concatenation of Patient last and first name separated by a comma and a space. Only the first 16 characters of the concatenated string are transmitted.

Example: “Doe, John.”


PN 4277303D 4-7


Cassette/Position Field:

Sequence Number:

1E Cassette/Position Field:

ID #1 Status Field:

Cassette/Position Status Field:


C A S S / P O S X X X X / X X 8 bytes CASS/POS output is cassette number/cassette position. ‘SECONDA’ - Manual sampling mode.


S E Q U E N C E X X X X X X 7 bytes Sequence Number


1 E C / P A X X X X X X 8 bytes Date Values:‘S’ - Manual sampling mode‘ ‘ - Automatic sampling mode.


I D 1 S T A T U S A 2 bytes Data Values: ‘P’ - positive ID. ‘E’ - edited ID. ‘ ‘ - not edited and not positive ID.

‘N’ - Auto-numbered.Precedence order is ‘E’, ‘N’, ‘P’ and ‘ ‘.


C / P S T A T U S A 2 bytes Data Values: ‘P’ - positive ID. ‘E’ - edited ID. ‘ ‘ - not edited and not positive ID.

Precedence order is: ‘E, ‘P’ and ‘ ‘.

PN 4277303D4-8


ToDo List Status:

CBC Parameter Group FieldsTable 4.4 identifies the field names, test types, and applicable format of CBC Parameter Group transmissions

Each field in the CBC Parameters Group will have the following format:

Result Format:**


W L S T A T U S A A A A A A A A 9 bytes See note below.*

*Data Values:‘GOOD’ = O.K. (Complete)‘PART ASP’ = Partial aspiration‘NO READ’ = Bar code not read‘NO MATCH’ = Match not found in ToDo list‘ ’ (20H) = Primary ID is cassette position and sample ID does not match preassigned ID

Table 4.4 CBC Parameters -- Group 2


WBC C, CD, CDR, CR, Edited XRBC C, CD, CDR, CR, R†, Edited X

HGB C, CD, CDR, CR, Edited XHCT C, CD, CDR, CR, Edited XMCV C, CD, CDR, CR, Edited XMCH C, CD, CDR, CR, Edited XMCHC C, CD, CDR, CR, Edited XRDW C, CD, CDR, CR, Edited XPLT C, CD, CDR, CR, Edited XPCT C, CD, CDR, CR, Edited XMPV C, CD, CDR, CR, Edited XPDW C, CD, CDR, CR, Edited X

X = Supported by the LH 750, “STKS 1G.1 + Retics” format.†For “Retic only” samples, only the RBC parameter is applicable. If the RBC parameter value is not

available, then it will not be transmitted and this group will have a field count of 00.


A A A A X X X X X X A A A See “Data Format” column.

See Below.**

6 char numeric data max.

3 char flag data max.

PN 4277303D 4-9


The parameter result will be transmitted in the Reporting Units with the extra digit criteria selected on the LH SeriesWorkstation.

Numeric Data: **

If the numeric data of each format (for example, xx.xx) does not contain a decimal number, then it contains one of the following: Flag Data: **

The flag data characters for the CBC Parameter Group Fields are as follows in Table 4.5. Table 4.5 lists all the possible flags generated on the LH Series Workstation and how they translate to the STKS “1G.1 + Retics” format.

DIFF Count Parameter Group FieldsTable 4.6 identifies the field names, test types, and applicable format of DIFF Count Parameter Group transmissions.

Result Comments

. . . . . Incomplete computation

+ + + + + Parameter overrange

- - - - - Parameter voteout

Table 4.5 Flag Data for CBC Parameter Group Fields

Comments

STKS 1G.1 Formats Translation (3 characters)

Review parameter. RExceeds reference interval high limit HExceeds reference interval low limit LExceeds action high limit HExceeds action low limit LExceeds critical high limit HExceeds critical low limit LOperator edited result ERecalculated result from edited result eMCV threshold failure * RDelta check failedPartial aspiration/blood detector disabled RResult exceeds linearity* = Not supported by the “STKS 1G.1 + Retics“ format.

Table 4.6 DIFF Count Parameters -- Group 3


LY# CD, CDR, Edited X

MO# CD, CDR, Edited X

NE# CD, CDR, Edited X

PN 4277303D4-10


This group is applicable to “CBC/DIFF” and “CBC/DIFF/Retic” sample runs. For all other sample runs, this group will have a field count of 00.

Each field in the DIFF Count Parameters Group will have the following format:

Result Format: **

The parameter result will be transmitted in the Reporting Units with the extra digit criteria selected on the LH Series Workstation.

Numeric Data: **

If the numeric data of each format (for example, xx.xx) does not contain a decimal number, then it contains one of the following:

Flag Data: **

The flag data characters for the DIFF Count Parameter Group fields are as follows in Table 4.7:

EO# CD, CDR, Edited X

BA# CD, CDR, Edited X

NRBC# CD, CDR, Edited

X = Supported by the “STKS 1G.1 + Retics ” format.



See Below.**

Result Comments


: : : : : Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)

????? Invalid data: could not calculate DIFF#.

+ + + + + WBC parameter overrange or sum of DIFF# parameters is greater than 500.0 x 106 cells/µL (whole blood) or sum of DIFF# parameters is greater than 600.0 x 106 cells/µL (predilute).




PN 4277303D 4-11


DIFF Percent Parameter Group Fields Table 4.8 identifies the field names, test types, and applicable format of DIFF Percent Parameter Group transmissions.

This group is applicable to “CBC/DIFF” and “CBC/DIFF/Retics” sample runs. For all other sample runs, this group will have a field count of 00.

Table 4.7 Flag Data for Diff Count Parameter Group Fields

Comments


Review parameter: WBC threshold failure or WBC exceeds linearity or sum of DIFF% results exceeds 100.

R

Exceeds reference interval high limit HExceeds reference interval low limit LExceeds action high limit HExceeds action low limit LExceeds critical high limit HExceeds critical low limit LOperator edited result ERecalculated result from edited result eParameter value exceeds Delta check limitsPartial aspiration/blood detector disabled RResult exceeds linearity

Table 4.8 DIFF Percent Parameters -- Group 4


LY% CD, CDR, Edited X

MO% CD, CDR, Edited X

NE% CD, CDR, Edited X

EO% CD, CDR, Edited X

BA% CD, CDR, Edited X

NRBC% CD, CDR, Edited

X = Supported by the “STKS 1G.1 + Retics” format.

PN 4277303D4-12



Result Format:**


Numeric Data: **


Flag Data: **

The flag data characters for the DIFF Percent Parameter Group fields will be as follows in Table 4.9:



See Below.**

Result Comments



+ + + + + Sum of DIFF% parameter results is not equal to 100% +0.1, or DIFF# parameters is greater than 600.0 x 106 cells/µL (predilute).

Table 4.9 Flag Data for DIFF Percent Parameter Group Fields

Comments


Review parameter R

Exceeds reference interval high limit H

Exceeds reference interval low limit L

Exceeds action high limit H

Exceeds action low limit L

Exceeds critical high limit H

Exceeds critical low limit L

Operator edited result E

Recalculated result from edited result e

Delta check failed



PN 4277303D 4-13


Comment Group FieldsTable 4.10 identifies the field names, test types, and applicable format of Comment Group transmissions.

.

The Comment Group can have a variable number of fields which consist of a variable number of bytes. Each Comment field is made up of an ASCII string identifying the appropriate flag.

Flag Group FieldsTable 4.11 identifies the field names, test types, and applicable format of Flag Group transmissions.

Note: This group will always be transmitted and will only be sent once per sample transmission.

The Flag Group can have a variable number of fields that consist of a variable number of bytes. There are no Tags associated with a Flag field. Each Flag field is made up of an ASCII string identifying the appropriate flag.

Table 4.12 lists all the possible flags generated on the LH Series Workstation and how they translate to the “STKS 1G.1 + Retics” format. For example, a Low Event # suspect flag

Partial aspiration/blood detector disabled R

Result exceeds linearity

Table 4.10 Comment -- Group 5


Comment C, CD, CDR, CR, R X

X = Supported by the STKS 1G.1 + Retics ” format.

Data Format Data Length Comments

X X X X X X X X • • • X X X X X X X 64 bytes maximum

Table 4.11 Flag -- Group 6


Variable C, CD, CDR, CR, R X


Data Format Data Length Comments

X X X X X X X X • • • X X X X X X X Variable


PN 4277303D4-14


message in LH 750 format is a Review message and an Abnormal WBC Pop message in “STKS 1G.1 + Retics ” format.

Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages

LH Series Workstation Message STKS 1G.1 + Retics

Suspect Flags

Low Event # Review Slide Abnormal WBC Pop

Verify Diff Review Slide Abnormal WBC Pop

Ne Blasts Blasts Abnormal WBC Pop

Ly Blasts Blasts Abnormal WBC Pop

Mo Blasts Blasts Abnormal WBC Pop

Imm. NE 1 Imm Grans/Bands 1 Abnormal WBC Pop

Imm. NE 2 Imm Grans/Bands 2 Abnormal WBC Pop

Variant LY Variant Lymphs Abnormal WBC Pop

NRBC NRBCs Abnormal RBC Pop

WBC Interference (N/A) (N/A)

RBC Interference (N/A) (N/A)

Dimorphic Reds Dimorphic RBC Pop Abnormal RBC Pop

Red Cell Agglutination RBC Agglutination Abnormal RBC Pop

Verify Retic (N/A) Verify Retic

Abnormal Retic Pattern (N/A) (N/A)

Platelet Clumps Platelet Clumps Abnormal PLT Pop

Giant Platelets Giant Platelets Abnormal PLT Pop

Inconsistent Diff CD (N/A) (N/A)

Abnormal Subsets Abnormal Subsets (N/A)

WBC Exceeds Linearity (N/A) (N/A)

Definitive Flags

Leukopenia Leukopenia Abnormal WBC Pop

Leukocytosis Leukocytosis Abnormal WBC Pop

Neutropenia % Neutropenia % Abnormal WBC Pop

Neutropenia # Neutropenia # Abnormal WBC Pop

Neutrophilia % Neutrophilia % Abnormal WBC Pop

Neutrophilia # Neutrophilia # Abnormal WBC Pop

Lymphopenia % Lymphopenia % Abnormal WBC Pop

Lymphopenia # Lymphopenia # Abnormal WBC Pop

Lymphocytosis % Lymphocytosis % Abnormal WBC Pop

Lymphocytosis # Lymphocytosis # Abnormal WBC Pop

Monocytosis % Monocytosis % Abnormal WBC Pop

PN 4277303D 4-15


Monocytosis # Monocytosis # Abnormal WBC Pop

Eosinophilia % Eosinophilia % Abnormal WBC Pop

Eosinophilia # Eosinophilia # Abnormal WBC Pop

Basophilia % Basophilia % Abnormal WBC Pop

Basophilia # Basophilia # Abnormal WBC Pop

Anemia Anemia Abnormal RBC Pop

Anisocytosis Anisocytosis Abnormal RBC Pop

1+ Anisocytosis 1+ Anisocytosis Abnormal RBC Pop



Microcytosis Microcytosis Abnormal RBC Pop

1+ Microcytosis 1+ Microcytosis Abnormal RBC Pop



Macrocytosis Macrocytosis Abnormal RBC Pop

1+ Macrocytosis 1+ Macrocytosis Abnormal RBC Pop



Hypochromia Hypochromia Abnormal RBC Pop

1+ Hypochromia 1+ Hypochromia Abnormal RBC Pop



(N/A) 1+ Poikilocytosis Abnormal RBC Pop



Erythrocytosis Erythrocytosis Abnormal RBC Pop

Pancytopenia Pancytopenia Abnormal WBC Pop and

Abnormal RBC Pop and

Abnormal PLT Pop

Reticulocytosis (N/A) (N/A)

Thrombocytopenia Thrombocytopenia Abnormal PLT Pop

Thrombocytosis Thrombocytosis Abnormal PLT Pop

Small Platelets Small Platelets Abnormal PLT Pop

Large Platelets Large Platelets Abnormal PLT Pop

H&H Check Failed (N/A) (N/A)

Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages (Continued)


PN 4277303D4-16


Parameter Flags

. . . . . . . . . . Abnormal WBC Pop and/or

Abnormal RBC Pop and/or

Abnormal PLT Pop and/or

Verify Retic

+ + + + + + + + + + Abnormal WBC Pop and/or


Abnormal PLT Pop

- - - - - - - - - - Abnormal WBC Pop and/or



Verify Retic

????? ????? Abnormal WBC Pop and/or



Verify Retic

: : : : : : : : : : Abnormal WBC Pop and/or



Verify Retic

R R (N/A)

H H (N/A)

L L (N/A)

E E Edited Data

e e Edited Data

D (N/A) (N/A)

+ +++++ Abnormal WBC Pop and/or



Verify Retic

Other Flags

Edited Data Edited Data (N/A)

Preliminary Report Preliminary Report (N/A)

Additional Test Report (N/A) (N/A)

Incomplete Collation Collate Failed (N/A)

SYSTEM ALARM (N/A) (N/A)



PN 4277303D 4-17


Note: These flags will be produced only for the research parameters that are transmitted.

Demographics Group FieldsTable 4.13 identifies the field names, test types, and applicable format of Demographics Group transmissions.

Note: This group will be transmitted only once per sample transmission.

Completed Report (N/A) (N/A)

Blood Detector Disabled (N/A) (N/A)

Sample validated (N/A) (N/A)

Sample not validated (N/A) (N/A)

AutoValidated (N/A) (N/A)



STKS Messages

PCT IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES

PDW IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES

Table 4.13 Demographics -- Group 7


Date of Birth C, CD, CDR, CR, R X

User Field #1 C, CD, CDR, CR, R X



Sex C, CD, CDR, CR, R X

Location C, CD, CDR, CR, R X

Physician C, CD, CDR, CR, R X

User Date C, CD, CDR, CR, R X

User Time C, CD, CDR, CR, R X


PN 4277303D4-18


Date of Birth Field:

User Field #1:

User Field #2:

User Field #3:

Sex Field:

Location Field:


B I R T H N N / N N / N N N N 11 bytes Date of birth output is month/day/year, regardless of the system date configuration.

The Date Of Birth Year is Always 4 Characters.


U F 1 X X • • • X X X 17 bytes






S E X A 2 bytes Data Value:

‘M’ Male

‘F’ Female

‘ ’ (20H) Unknown


L O C A T I O N X X X • • • X X X 17 bytes

PN 4277303D 4-19


Physician Field:

User Date Field:

User Time Field:

DF1 DataPlot Group FieldsThe 1G.1 + Retics format does not support transmission of this group. This group will be transmitted with field count of 00.

DF2 DataPlot Group FieldsThe 1G.1 + Retics format does not support transmission of this group. This group will be transmitted with field count of 00.

DIFF (VCS) Histogram Group Fields The 1G.1 + Retics format does not support transmission of this group. This group will be transmitted with field count of 00.


P H Y S I C I A N X X X • • • X X X 22 bytes Concatenation of Physician last and first name separated by a comma and a space. Only the first 22 characters of the concatenated string are transmitted. Example: “Smith, Peter.”


U D A T E N N / N N / N N 9 bytes Sample Drawn Date. Output format is month/day/year, regardless of the system date configuration.


U T I M E N N : N N : N N 9 bytes Sample Drawn Time. Output is on 24 hours, regardless of the system time configuration. Time output is hours:minutes: seconds.

PN 4277303D4-20


RBC Histogram Group FieldsTable 4.14 identifies the field names, test types, and applicable format of RBC Histogram Group Field transmissions.

If RBC histogram graphic transmission is disabled, this group is transmitted with a field of 00.

RBC Histogram Field:

PLT Histogram Group Fields Table 4.15 identifies the field names, test types, and applicable format of PLT Histogram Group Field transmissions.

If PLT histogram graphic transmission is disabled, this group is transmitted with a field of 00.

Note: When the PLT histogram graphic transmission is enabled the data in the PLTF field is always sent. Ignore the data sent in the PLTF field if an R flag is present for the PLT parameter sent in the CBC Parameter Group.

PLT Histogram Field:

Table 4.14 RBC Histogram -- Group 11


RBC Histogram C, CD, CDR, CR X



R B C H H H H • • • H H H 512 bytes See Chapter 8 for graphic reconstruction.

Table 4.15 PLT Histogram -- Group 12


PLT Histogram C, CD, CDR, CR X

PLT Fit Histogram C, CD, CDR, CR X



P L T H H H H • • • H H H 256 bytes See Chapter 8 for graphic reconstruction.

PN 4277303D 4-21


PLT Fit Histogram Field:

RETIC Parameters Group FieldsTable 4.16 identifies the field names, test types, and applicable format of RETIC Parameters Group Field transmissions.

Retic Run Date Field:


P L T F H H H • • • H H H 512 bytes See Chapter 8 for graphic reconstruction.

Table 4.16 RETIC Parameters -- Group 13


Retic Run Date CDR, CR, R X

Retic Run Time CDR, CR, R X

Retic ID CDR, CR, R X

Retic Cassette Position CDR, CR, R X

RET% CDR, CR, R, Edited X

RET# CDR, CR, R, Edited X

MRV CDR, CR, R, Edited X

MI (now called IRF) CDR, CR, R, Edited X

X = Supported by the LH 750, STKS 1G.1 + Retics ” format.


R E T D A T E N N / N N / N N 9 bytes Retic Run Date. Output is month/day/year, regardless of the system time configuration. Note: In the case of a “CBC/Retic” or “CBC/Diff/Retic” sample run, the Date and Time of the General Information group are duplicated in the RETDATE and RETTIME fields.

PN 4277303D4-22


Retic Run Time Field:

Retic ID Field:

**Note: In the case of a “CBC/Retic” or “CBC/DIFF/Retic” sample run, the Sample ID transmitted in the ID1 field is duplicated in this field.

Retic Cassette/Position Field:

Retic Parameter Results Field:

Result Format:**

1. The parameter result will be transmitted in the Reporting Units with the extra digit criteria selected on the LH Series Workstation.


R E T T I M E N N : N N : N N 9 bytes Retic Run Time. Output is on 24 hours, regardless of the system time configuration. Time output is hours:minutes:seconds.Note: In the case of a “CBC/Retic” or “CBC/Diff/Retic” sample run, the Date and Time of the General Information group are duplicated in the RETDATE and RETTIME fields.


R I D X X • • • X X X 17 bytes See Note**


R C A S S / P O S X X X X / X X 8 bytes This field only applies to the 1G.1+ Retics host transmission protocol.



See Below.**



PN 4277303D 4-23


Numeric Data: **


Flag Data: **

The flag data characters for the Retic Parameter Group fields will be as follows in Table 4.17:

If a Retic parameter is disabled, results for that parameter will NOT be transmitted. The IRF parameter result is transmitted with the label MI for backward compatibility reasons.

DF5 LS DataPlot Group Fields1G.1 + Retics format does not support transmission of this group. This group will be transmitted with field count of 00.

DF6 OP DataPlot Group Fields1G.1 + Retics format does not support transmission of this group. This group will be transmitted with field count of 00.

Result Comments



????? Invalid data: could not calculate RET#.+ + + + + Retic parameter result overrange

RET# > 999.9 x 106cells/µL

RET% > 100%

Table 4.17 Flag Data for Retic Parameter Group Fields

LH 750 Workstation Comments STKS 1G.1 + Retics

Review parameter R

Exceeds reference interval high limit H

Exceeds reference interval low limit L

Exceeds action high limit H

Exceeds action low limit L

Exceeds critical high limit H

Exceeds critical low limit L

Operator edited result E

Recalculated result from edited result e

Delta check failed

Partial aspiration/blood detector disabled R

Result exceeds linearity

PN 4277303D4-24

LH SERIES WORKSTATION TO HOST COMMUNICATIONSLH 750 WORKSTATION FORMAT 4

RETIC Histogram Group1G.1 + Retics format does not support transmission of this group. This group will be transmitted with field count of 00.

4.3 LH 750 WORKSTATION FORMAT

Message StructureThe Presentation deals with the high level format of the message. The data bytes of the transmission blocks, when collected together, exhibit the following high level format.

Preamble: The preamble marks the beginning of a message.

Transmission Identification:

The ASCII character "S" marks the beginning of the transmission.

TRANSMISSION TEST COUNT defines the number of tests that will be transmitted in this particular transmission. Not all Test Types are sent in all transmissions.

Each Transmission Test is identified by the order in which it appears after the preamble. The transmission test will never be transmitted out of order.

The Transmission Tests are up to 32 characters long. Table 4.18 lists the available Transmission Tests and Test IDs by Test Type.

PREAMBLE TRANSMISSION IDENTIFICATION TEST a IDENTIFICATION GROUP x1 • • • •• • • •

GROUP xn • • •

• • • TESTz IDENTIFICATION GROUP x1 • • • •• • • •

GROUP xn POSTAMBLE

CR

LF

CR

LF

CR

LF

CR

LF

CR

LF

CR

LF

-

-

-

-

-

-

-

-

-

-

-

-

-

-

CR

LF

S TRANSMISSION

TESTCOUNT

CR

LF

TRANSMISSION

TEST1

CR

LF O O O

TRANSMISSION

TESTN

CR

LF

Table 4.18 Transmission Tests and Test IDs by Test Type

Test Type Transmission Tests Test IDs

C “CBC” “CBC”CD “CBC”, “DIFF” “CBC”, “DIFF”R “RETIC” “RETIC”CR “CBC”, “RETIC” “CBC”, “RETIC”CDR “CBC”, “DIFF”, “RETIC” “CBC”, “DIFF”, “RETIC”C & CD Control “5CC” “CBC”, “DIFF”R Control “RETICC” “RETIC”CR Control “RETICC” “CBC”, “RETIC”CDR Control “RETICC” “CBC”, “DIFF”, “RETIC”CD Latex “DLATEX” “CBC”, “DIFF”

PN 4277303D 4-25

LH SERIES WORKSTATION TO HOST COMMUNICATIONSLH 750 WORKSTATION FORMAT

Currently only manually entered data is transmitted.

Test Identification:

The ASCII character "T" marks the beginning of a test identification.

TEST ID, see Table 4.18.

GROUP COUNT defines the number of groups of data in this particular TEST Identification.

Group:

The ASCII character "G" marks the beginning of a group. A group follows the test identification fields.

GROUP NUMBER defines the group number. See Group Definition.

FIELD COUNT defines the number of fields in a particular group. See Field Count.

FIELD defines a field within a group. See Fields.

Note: When there is no data for a Group, the Group will not be sent.

Test Type Count:The transmission identification section has a Test Type Count that identifies the number of tests contained within the transmission. Valid test type count can be from 0-255. The test type count uses two characters to give the ASCII representation of the hexadecimal value.

Group Count:Each test identification has a Group Count that identifies the number of groups contained within the test. Valid group counts can be from 0-255. The group count uses two characters to give the ASCII representation of the hexadecimal value.

Group Number:Each group identifier has a Group Number that identifies the group number contained within

CDR Latex “LATEXC” “CBC”, “DIFF”, “RETIC”Retic Latex “RLATEX” “RETIC”

T TESTID

CR

LF

GROUPCOUNT

CR

LF

G GROUP

NUMBER

CR

LF

FIELD

COUNT

CR

LF

FIELD

1

CR

LF • • • •

FIELD

N

CR

LF

MSCHAR

LSCHAR


MSCHAR

LSCHAR


Table 4.18 Transmission Tests and Test IDs by Test Type

PN 4277303D4-26


the group identifier. Valid group number can be from 0-255. The group number uses two characters to give the ASCII representation of the hexadecimal value.

Field Count:Each Group has a Field Count that identifies the number of fields contained within the Group. Valid field counts can be from 0-255. The field count uses two characters to give the ASCII representation of the hexadecimal value.

Fields:Each Group is made up of variable length fields which may or may not be padded with SP (20H), or HT (09H) characters. A field may have a variable length tag preceding the data, separated by one or more SP (20H), or HT (09H) characters. Please note that all field transmissions will include a SP between the tag and data format (this will be implied in the following sections), as illustrated below:

The data portion of a field will only contain ASCII characters in the range of 20H to 7EH inclusive. Heading 6.2, VALID HOST COMMUNICATIONS ASCII CODES identifies the valid ASCII characters. Fields are separated with a CR (0DH) and an LF (0AH) character.

Note: A field's position within a Group must not be used to identify it. Fields within a Group may appear in any order or may be omitted. The CR/LF should be used to find fields and the Tag used to identify it.

ALL FIELDS ARE OF MAXIMUM 32 CHARACTERS LONG UNLESS OTHERWISE NOTED.

The following abbreviations are used to describe the fields:

SEP - one or more SP (20H) or HT (09H) characters.

The syntax for the Data Format of a field is:

MSCHAR

LSCHAR


MSCHAR

LSCHAR


Tag Sep Data Format

T I M E N N : N N : N N

A - Alpha characters (a..z and A..Z and space) or (61H..7AH and 41H..5AH and 20H)

N - Numeric characters (0..9 and + - .) or (30H..39H and 2BH 2DH 2EH)

X - Printable characters (20H..7EH)

H - Hexadecimal characters

(0..9 and A..F)

O - Other characters (CR and LF) or (0DH and 0AH)

PN 4277303D 4-27


Postamble:

The postamble marks the end of the current message.

There are 24 groups defined in the current implementation of the LH 750 Workstation Format. Future revisions of LH Series Workstation may include additional groups. Any future additions will not disrupt the order of the groups as currently defined.

Group Definition The abbreviations used to identify tests the group applies to are:

Table 4-19 lists the groups transmitted under 2A, 3A, GEN•S, and LH 750 Workstation formats.

CR

LF

CR

LF

-

-

-

-

-

-

-

-

-

-

-

-

-

-

CR

LF

C CBC

D DIFF

R RETICS

Table 4.19 Transmitted Groups

Group Number Group Name Tests LH 750 Workstation

1 General Information C D R X, *

2 CBC Parameters C X

3 DIFF Count Parameters D X

4 DIFF Percent Parameters D X

5 Comments C D R X, *

6 Definitive Flags C D R X, *

7 Suspect Flags C D R X, *

8 Conditional Flags C D R X, *

9 Other Flags C D R X, *

10 Demographics C D R X, *

14 RBC Histograms C X

15 PLT Histograms C X

16 RETIC Parameters R X

26 WBC Threshold C X

27 WBC Threshold Full Histogram C X

31 DIFF Latex Parameters D X

32 Retic Latex Parameters R X

34 Control Information C D R X

35 Control CBC Parameters C X

36 Control DIFF Count Parameters D X

37 Control DIFF Percent Parameters D X

PN 4277303D4-28


Details of Group fields are defined in the following sections.

General Information Group FieldsTable 4.20 identifies the field names, test types, and applicable format of General Information transmissions.

38 Control Retics Parameters R X

50 DIFF Graphics D X

51 Retic Graphics R X

X = Supported by the LH 750, Workstation format.* = Indicates that the group information will not be repeated with every test.

Table 4.19 Transmitted Groups

Group Number Group Name Tests LH 750 Workstation


Field Name Test Type LH 750 Workstation

Run Date C, CD, CDR, CR, R X

Run Time C, CD, CDR, CR, R X

Sample Age C, CD, CDR, CR, R X

Sample ID C, CD, CDR, CR, R X

Pre Assigned Sample ID C, CD, CDR, CR, R X

Cassette / Position C, CD, CDR, CR, R X

Pre Assigned Cassette / Position C, CD, CDR, CR, R X

Sample ID Status C, CD, CDR, CR, R X

Cassette / Position Status C, CD, CDR, CR, R X

Sample Status C, CD, CDR, CR, R X

Instrument ID C, CD, CDR, CR, R X

Operator ID C, CD, CDR, CR, R X

Dilution Factor C ~ X

Body Fluid C ~ X

X = Supported by the LH 750, Workstation format.~ = New for the LH 750 Workstation format.

PN 4277303D 4-29


Run Date Field:

Run Time Field:

Sample Age Field:

Sample ID Field:


D A T E N N / N N / N N 8 bytes Run Date. Output is month/day/year, regardless of the system date configuration.


T I M E N N : N N : N N 8 bytes Run Time. Output is on 24 hours, regardless of the system time configuration. Time output is hours:minutes:seconds.


S A N N N N : N N : N N 11 bytes Age of sample at run time. Format is DD HH:MM:SS. Computed from Draw Date and Draw Time. This field will not appear if draw date and time are invalid or after run date and time.


I D 1 X X X • • • X X X X X 16 bytes Bar code read on tube by instrument at aspiration time, entered on the Diluter keypad for Manual mode aspiration, or ID generated by Auto-numbering feature.

PN 4277303D4-30


Pre Assigned Sample ID Field:


Pre-Assigned Cassette/Position Field:

Sample ID Status Field:


P I D 1 X X X • • • X X X 16 bytes PRE ASSIGNED

SAMPLE ID


C A S S P O S A C C C C P P 7 bytes CASS/POS output is cassette number (‘C’)/ cassette position (‘P’).

Data values for 'A' are:

‘A’ for Automatic.

‘M’ for Manual.


P C A S S P O S C C C C P P 6 bytes CASS/POS entered through ToDo list. Format is cassette number (‘C’)/ cassette position (‘P’). The sample mode is not specified at preassignment time.


I D 1 S T A T U S A 1 byte Data Values:

‘P’ - positive ID

‘E’ - edited ID

‘N’ - Auto-numbered ID

‘ ’ (20H) - not edited, not auto-numbered and not part of positive identification scheme.

Precedence order is: ‘E’, ‘N’, ‘P’ and ‘ ’ (20H).

PN 4277303D 4-31


Cassette/Position Status Field:

Sample Status:

Instrument ID:

Operator ID:

Dilution Factor::


C / P S T A T U S A 1 byte Data Values:

‘P’ - positive ID

‘E’ - edited ID

‘ ’ (20H) - not edited and not part of positive identification scheme.

Precedence order is: ‘E’, ‘P’ and ‘ ’ (20H).


S A S T A T U S X X • • • X X X 8 bytes See Table 4.21.


I N S T A A A • • • A A 16 bytes Identifies the LH 750 instrument this sample was run on.


O P R A A A • • • A A 14 bytes Identifies the user logged on the LH 750 Workstation at the time this sample run was analyzed.


D F A C T N N N N N 5 bytes Identifies amount by volume by which sample is diluted.This field is always transmitted. All have the default value of 1.0 when used. The factor is applied to transmitted parameter values.

PN 4277303D4-32


Body Fluid::

CBC Parameters Group FieldsTable 4.22 identifies the field names, test types, and applicable format of CBC Parameter transmissions.


B O D F A A A • • A A A 16 bytes This field is only transmitted when the sample is a body fluid and then only if “Enable Body Fluid Tag” in System Setup - (Communication) is checked. Current data is always “Body Fluid”. The parameters displayed are WBC, and RBC. (For LH 750 ONLY)

Table 4.21 Possible Values for Sample Status

Possible Value LH 750 Workstation Comments

PART ASP X Partial aspiration

NO READ X Bar code not read

NO MATCH X Match not found in ToDo list

MISMATCH X Non positive ID mismatch

INC COL X Incomplete collation

COMPLETE X No ToDo List errors

X in the LH 750 Workstation column = Supported by the LH 750 Workstation format.

Table 4.22 CBC Parameters -- Group 2


WBC C, CD, CDR, CR, Edited XRBC C, CD, CDR, CR, Edited XHGB C, CD, CDR, CR, Edited XHCT C, CD, CDR, CR, Edited XMCV C, CD, CDR, CR, Edited XMCH C, CD, CDR, CR, Edited XMCHC C, CD, CDR, CR, Edited XRDW C, CD, CDR, CR, Edited XPLT C, CD, CDR, CR, Edited XPCT C, CD, CDR, CR, Edited XMPV C, CD, CDR, CR, Edited XPDW C, CD, CDR, CR, Edited X

PN 4277303D 4-33


Each field in the CBC Parameters Group will have the following format:

Result Format: **


Numeric Data: **


Flag Data: **

The flag data characters for the CBC Parameter Group Fields are as follows in Table 4.23:

X = Supported by the LH 750 Workstation format.


A A A • • A X X • • • X X A A A A See “Data Format” column.

See Below.**

Result Comments


+ + + + + Parameter overrange

- - - - - Parameter voteout


LH 750 Workstation Flags 4 (characters) Comments

R Review parameterH Exceeds reference interval high limitL Exceeds reference interval low limit

a H Exceeds action high limita L Exceeds action low limitc H Exceeds critical high limitc L Exceeds critical low limit

E Operator edited result

Table 4.22 CBC Parameters -- Group 2 (Continued)




1 char separator min.

PN 4277303D4-34


DIFF Count Parameters Group FieldsTable 4.24 identifies the field names, test types, and applicable format of DIFF Count Parameter transmissions.


Note: NRBC% will only be present if the NRBC Suspect Flag was triggered.

Result Format: **

e Recalculated result from edited result* MCV threshold failure

D Delta check failedP Partial aspiration/blood detector disabled

+ Result exceeds linearityV Single Aperture Voteout (LH 500 Only)v Result calculated from Single Aperture Voteout



LY# CD, CDR, Edited X

MO# CD, CDR, Edited X

NE# CD, CDR, Edited X

EO# CD, CDR, Edited X

BA# CD, CDR, Edited X

NRBC# CD, CDR, Edited X




See Below.**


LH 750 Workstation Flags 4 (characters) Comments




PN 4277303D 4-35



Numeric Data: **


Flag Data: **

The flag data characters for the DIFF Count Parameter Group fields are as follows in Table 4.25:

Result Comments



????? Invalid data: could not calculate DIFF#.+ + + + + WBC parameter overrange or sum of DIFF# parameters is greater than 500.0 x

106 cells/µL (whole blood) or sum of DIFF# parameters is greater than 600.0 x 106 cells/µL (diluted).

Table 4.25 Flag Data for DIFF Count Parameter Group Fields

LH 750 Workstation Flags (4 characters) Comments

R Review parameter: WBC threshold failure or WBC exceeds linearity or sum of DIFF% results exceeds 100.

H Exceeds reference interval high limitL Exceeds reference interval low limit

a H Exceeds action high limita L Exceeds action low limitc H Exceeds critical high limitc L Exceeds critical low limit

E Operator edited resulte Recalculated result from edited result

D Parameter value exceeds Delta check limitsP Partial aspiration/blood detector disabled

+ Result exceeds linearityv Result calculated from Single Aperture Voteout (For LH 500 ONLY)

PN 4277303D4-36


DIFF Percent Parameters Group FieldsTable 4.26 identifies the field names, test types, and applicable format of DIFF Percent Parameter transmissions.

Each field in the DIFF Percent Parameters Group will have the following format:

Result Format: **


Numeric Data: **


Flag Data: **

The flag data characters for the DIFF Percent Parameter Group fields will be as follows in Table 4.27:

Table 4.26 DIFF Percent Parameters -- Group 4

Field Name Test Type GEN•S Workstation

LY% CD, CDR, Edited X

MO% CD, CDR, Edited X

NE% CD, CDR, Edited X

EO% CD, CDR, Edited X

BA% CD, CDR, Edited X

NRBC% CD, CDR, Edited X




See Below.**

Result Comments



+ + + + + Sum of DIFF% parameter results is not equal to 100% +0.1, or DIFF# parameters is greater than 600.0 x 106 cells/µL (predilute).




PN 4277303D 4-37


Comment Group FieldsTable 4.28 identifies the field names, test types, and applicable format of Comment Group Field transmissions.


The Comment Group can have a variable number of fields which consist of a variable number of bytes. Each Comment field is made up of an ASCII string identifying the appropriate flag.

When WBC correction is performed, the Uncorrected WBC will be printed at the beginning of the Comments field as UWBC=XXXX. For non-English applications, the UWBC will be translated into the language being used. (For LH 750 ONLY)



R Review parameter

H Exceeds reference interval high limit

L Exceeds reference interval low limit

a H Exceeds action high limit

a L Exceeds action low limit

c H Exceeds critical high limit

c L Exceeds critical low limit


e Recalculated result from edited result

D Delta check failed

P Partial aspiration/blood detector disabled

+ Result exceeds linearity

Table 4.28 Comment -- Group 5


Comment C, CD, CDR, CR, R X



C O M M E N T X X X • • • X X X 254 bytes STKS format is only 64 characters long.

PN 4277303D4-38


Definitive Flags Group FieldsTable 4.29 identifies the field names, test types, and applicable format of Definitive Flag transmissions.


** The possible definitive flags generated on the LH Series Workstation are:

Table 4.29 Definitive Flags -- Group 6


Definitive Flag C, CD, CDR, CR, R X



D E F I N I T X X X • • • X X X Up to 128 bytes

See Below.**

LH 750 Workstation MessagesLeukopeniaLeukocytosisNeutropenia %Neutropenia #Neutrophilia %Neutrophilia #Lymphopenia %Lymphopenia #Lymphocytosis %Lymphocytosis #Monocytosis %Monocytosis #Eosinophilia %Eosinophilia #Basophilia %Basophilia #AnemiaAnisocytosis1+ Anisocytosis2+ Anisocytosis3+ AnisocytosisMicrocytosis1+ Microcytosis2+ Microcytosis3+ MicrocytosisMacrocytosis1+ Macrocytosis2+ Macrocytosis3+ Macrocytosis

PN 4277303D 4-39


Suspect Flags Group FieldsTable 4.30 identifies the field names, test types, and applicable format of Suspect Flag transmissions.


Note: When the LH System generates a “Cellular Interference” suspect message, the Host transmission will replace the message with “WBC Interference”.

** The possible Suspect flags generated on the LH Series Workstation are:

Hypochromia1+ Hypochromia2+ Hypochromia3+ HypochromiaErythrocytosisPancytopeniaReticulocytosisThrombocytopeniaThrombocytosisSmall PlateletsLarge PlateletsH & H Check Failed

Table 4.30 Suspect Flags -- Group 7


Suspect Flag C, CD, CDR, CR, R XX = Supported by the LH 750 Workstation format.


S U S P E C T X X X • • • X X X Up to 128 bytes

See Below.**

LH 750 Workstation MessagesLow Event #Verify DiffNe BlastsLy BlastsMo BlastsImm NE 1 Imm NE 2 Variant LY NRBCDimorphic RedsRed Cell AgglutinationVerify Retic

(Continued) LH 750 Workstation Messages (Continued)

PN 4277303D4-40


Conditional Flags Group FieldsTable 4.31 identifies the field names, test types, and applicable format of Conditional Flag transmissions.

Note: This group will be transmitted with the first test only in each sample transmission.

**The possible Conditional flags generated on the LH Series Workstation are:

AutoValidation:

The auto validation flags show the validation status of test requests for a sample on printouts and within the results transmission. A functional example of the validation codes is described below.

A specimen is run in CDR mode with a CDR report format requested. Suppose results are acceptable for CBC and Retic, but not for the Diff (e.g., a Decision Rule fails, such as “If Blast [suspect flag], Perform Manual Diff”).

Abnormal Retic PatternPlatelet ClumpsGiant PlateletsWBC InterferenceRBC InterferenceWBC Exceeds Linearity

Table 4.31 Conditional Flags -- Group 8


Conditional Flag C, CD, CDR, CR, R XX = Supported by the LH 750 Workstation format.


C O N D I T I O N X X X • • • X X X Up to 128 bytes

See Below.**

LH 750 Workstation Messages

Edited DataPreliminary ReportAdditional Test ReportIncomplete CollationSYSTEM ALARMCompleted ReportBlood Detector DisabledSample validatedSample not validatedAutoValidated

LH 750 Workstation Messages (Continued)

PN 4277303D 4-41


Note: The configuration of the Workstation affects the validation messages and codes.

The CBC and Retic test groups are AUTOVALIDATED, but the Diff group is NOT VALIDATED. There are two ways to indicate this. A conditional text message SAMPLE NOT VALIDATED would be transmitted. Additionally, validation codes C AV, R AV, D NV indicate the status of the individual test groups. The Diff can later be validated manually, resulting in SAMPLE VALIDATED, C AV, R AV, D V.

It is possible through use of the report format to have validation messages based on the report profile (report format). For example, using the scenario above, if a CBC report profile was requested, validation would be based solely on the results of the CBC group. For a complete explanation, refer to the LH Series Workstation on-line help.

One of the conditional text messages below will always be transmitted.

r Sample not validated r Sample validated r AutoValidated

If “Enable Validation Codes” in Sytem Setup - [Communications] is checked (as described in Heading 3.16, ENABLE VALIDATION CODES), the individual test group validation status will be transmitted based on the profile selected and test mode run. The following columns display the possible codes transmitted for the individual groups:

Validation Flags

AV AutoValidatedNV Not ValidatedV Validated (manually)

Validation CodesC CBC parameter blockD Diff parameter blockH H&H parameter blockR Retic parameter blockW WBC/Hgb/Plt parameter block

Sample Not Validated Sample Validated AutoValidated

C NV C V C AV

D NV D V D AV

R NV R V R AV

H NV H V H AV

W NV W V W AV

PN 4277303D4-42


Other Flags Group FieldsTable 4.32 identifies the field names, test types, and applicable format of Other Flag transmissions.

Note: This group will be transmitted only once in each sample transmission.

**The other possible flags generated on the LH Series Workstation are:

Note: These flags will be produced only for the research parameters that are transmitted.

DemographicsTable 4.33 identifies the field names, test types, and applicable format of Demographics Group Field transmissions.

Table 4.32 Other Flags -- Group 9


Other Flag C, CD, CDR, CR, R XX = Supported by the LH 750 Workstation format.


O T H E R X X X • • • X X X Up to 128 bytes

See Below.**

LH 750 Workstation Messages

HLR% IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES

HLR# IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES

PCT IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES

PDW IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES

MSCV IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES

Table 4.33 Demographics -- Group 10


Date of Birth C, CD, CDR, CR, R XUser Field #1 C, CD, CDR, CR, R XUser Field #2 C, CD, CDR, CR, R XUser Field #3 C, CD, CDR, CR, R XSex C, CD, CDR, CR, R XLocation C, CD, CDR, CR, R XPhysician C, CD, CDR, CR, R XSample Drawn Date C, CD, CDR, CR, R XSample Drawn Time C, CD, CDR, CR, R XPatient Identification C, CD, CDR, CR, R XPatient Last Name C, CD, CDR, CR, R XPatient First Name C, CD, CDR, CR, R X

PN 4277303D 4-43



Date of Birth Field:

User Field #1:

User Field #2:

User Field #3:

Sequence Number C, CD, CDR, CR, R XProfile C, CD, CDR, CR, R XNumber of slides made C, CD, CDR, CR, R XNumber of slides requested C, CD, CDR, CR, R XReport Profile C, CD, CDR, CR, R X


Table 4.33 Demographics -- Group 10 (Continued)



B I R T H N N / N N / N N N N 10 bytes Date of BirthOutput is month/day/year, regardless of the system date configuration.The Date Of Birth Year is Always 4 Characters.


U F 1 X X X • • • X X X 16 bytes





PN 4277303D4-44


Sex Field:

Location Field:

Physician Field:

Sample Drawn Date Field:

Sample Drawn Time Field:


S E X A 1 byte Data Value:

‘M’ Male

‘F’ Female

‘U’ Unknown


L O C A T I O N X X X • • • X X X 16 bytes


P H Y S I C I A N X X X • • • X X X Up to 22 bytes Concatenation of Physician’s last and first name separated by a comma and a space. Example: “Smith, Peter.”


D D N N / N N / N N 8 bytes Sample Drawn Date. Output is month/day/year, regardless of the system date configuration.


D T N N : N N : N N 8 bytes Sample Drawn Time. Output is on 24 hours, regardless of the system time configuration. Time output is hours:minutes:seconds.

PN 4277303D 4-45


Patient Identification:

Patient Last Name:

Patient First Name:

Sequence Number:

Number of Slides Made:

Number of Slides Requested:


P I D X X X • • • X X X 16 bytes


P L N X X X • • • X X X 16 bytes


P F N X X X • • • X X X 16 bytes


S E Q U E N C E X X X X X X 6 bytes


S M N 1 byte Indicates how many slides were made for that sample on the LH 750 SlideMaker. Possible values are: 0 or 1. If 0, this field is not sent.


S R N 1 byte Indicates how many slides were requested through preassignment in the ToDo list for that sample. Possible values are: 0 or 1.

PN 4277303D4-46


Report Profile Field:

Profile Selection:

Data transmission reflects profile selection. Parameters not in the selected profile will not be transmitted. Test blocks (e.g., CBC, DIFF, etc.) which are not in the current profile will not be transmitted. If a complete test block is omitted from the current profile, the General Information Block (for sample identification) will still be sent.

RBC Histogram Group FieldsTable 4.34 identifies the field names, test types, and applicable format of RBC Histogram Group Field transmissions.

If CBC histograms graphic transmission is disabled, this group will NOT be transmitted.

RBC Histogram Field:

PLT Histogram Group FieldsTable 4.35 identifies the field names, test types, and applicable format of PLT Histogram Group Field transmissions.



R P N N 2 bytes Report Profile possible values are from “01" to "10". Always transmitted.

Table 4.34 RBC Histogram -- Group 14


RBC Histogram C, CD, CDR, CR X



R B C H H H H • • • H H H 512 bytes See Chapter 8 for graphic construction.

Table 4.35 PLT Histogram -- Group 15


PLT Histogram C, CD, CDR, CR X

PLT Fit Histogram C, CD, CDR, CR X


PN 4277303D 4-47


PLT Histogram Field:

Note: When the PLT histogram graphic transmission is enabled the data in the PLTF field is always sent. Ignore the data sent in the PLTF field if an R flag is present for the PLT parameter sent in the CBC Parameter Group.

PLT Fit Histogram Field:

RETIC Parameters Group FieldsTable 4.36 identifies the field names, test types, and applicable format of RETIC Parameters Group Field transmissions.

Result Format: **


P L T H H H H • • • H H H 256 bytes See Chapter 8 for graphic construction.


P L T F H H H • • • H H H 512 bytes See Chapter 8 for graphic construction.

Table 4.36 RETIC Parameters -- Group 16


RET% CDR, CR, R, Edited X

RET# CDR, CR, R, Edited X

MRV CDR, CR, R, Edited X

MSCV CDR, CR, R, Edited X

IRF CDR, CR, R, Edited X

HLR% CDR, CR, R, Edited X

HLR# CDR, CR, R, Edited X




See Below.**




PN 4277303D4-48



Numeric Data: **


Flag Data: **

The flag data characters for the Retic Parameter Group fields will be as follows in Table 4.37:

MRV, MSCV, HLR%, HLR# and IRF results will be transmitted only if they have been enabled. If a Retic parameter is disabled, results for that parameter will NOT be transmitted.

The LH Series Workstation does not support transmission of MI and OTHER fields. These fields will NOT be transmitted.

Result Comments




RET# > 999.9 x 106 cells/µL

RET% > 100%

Table 4.37 Flag Data for Retic Parameter Group Fields


R Review parameter

H Exceeds reference interval high limit

L Exceeds reference interval low limit

a H Exceeds action high limit

a L Exceeds action low limit

c H Exceeds critical high limit

c L Exceeds critical low limit


e Recalculated result from edited result

D Delta check failed



v Result calculated from Single Aperture Voteout (For LH 500 ONLY)

PN 4277303D 4-49


WBC Threshold Group FieldsTable 4.38 identifies the field names, test types, and applicable format of the WBC Threshold Monitor Group Field transmissions.


WBC Threshold Monitor Field:

WBC Threshold Full Histogram Group FieldsThis group has the same description as WBC Threshold Group Fields with the exception that additional data elements are added.

Table 4.38.1 WBC Full Histogram Monitor Group 27

WBC Threshold Full Monitor Field:

DIFF Latex Parameters Group FieldsTable 4.39 identifies the field names, test types, and applicable format of DIFF Latex Parameters Group transmissions.

Table 4.38 WBC Threshold Monitor Group 26

Field Name Test Type LH 750S Workstation

WBC Threshold Monitor C, CD, CDR, CR X



W B C T H H H • • • H H H 112 bytes See Chapter 8 for graphic construction.

Field Name Test Type LH 750S Workstation

WBC Threshold Monitor C, CD, CDR, CR X



W B C F H H H • • • H H H 512 bytes See Chapter 8 for graphic construction.

Table 4.39 DIFF Latex Parameters -- Group 31


PRIMER F55, F57 X

V_MN F55, F57 X

C_MN F55, F57 X

S_MN F55, F57 X

PN 4277303D4-50


Each field in the DIFF Latex Parameters Group will have the following format:

Result Format: **


Numeric Data: **


Flag Data: **

The flag data characters for the DIFF Latex Parameter Group fields will be as follows as in Table 4.40:

V_CV F55, F57 X

C_CV F55, F57 X

S_CV F55, F57 X



A A A • • A X X • • • X X A See “Data Format” column.

See Below.**

Result Comments



Table 4.40 Flag Data for DIFF Latex Parameters Group Fields

LH 750 Workstation Flags (1 character) Comments

H Greater than expected value range.

L Less than expected value range.

Table 4.39 DIFF Latex Parameters -- Group 31




PN 4277303D 4-51


RETIC Latex Parameters Group FieldsTable 4.41 identifies the field names, test types, and applicable format of RETIC Latex Parameters Group transmissions.

Each field in the RETIC Latex Parameters Group will have the following format:

Result Format: **


Numeric Data:**


Flag Data: **

Table 4.41 RETIC Latex Parameters -- Group 32

Field Name Test TypeLH 750 Workstation

PRIMER F56, F57 X

V_MN F56, F57 X

C_MN F56, F57 X

S_MN F56, F57 X

V_CV F56, F57 X

C_CV F56, F57, X

S_CV F56, F57 X



A A A • • A X X • • • X X A See “Data Format” column.

See Below.**

Result Comments






PN 4277303D4-52


The flag data characters for the RETIC Latex Parameter Group fields will be as follows as in Table 4.42:

Table 4.43 identifies the field names, test types, and applicable format of Control Information Group transmissions.

Run Number Field:

The LH Series Workstation does not support transmission of this field. This field will NOT be transmitted.

Lot Number Field:

User Control Name Field:

Table 4.42 Flag Data for RETIC Latex Parameters Group Fields

LH 750 Workstation Flags (1 character) Comments

H Greater than expected value range.

L Less than expected value range.

Table 4.43 Control Information -- Group 34


Run number C, CD, CDR, CR, R, LATEX *

Lot number C, CD, CDR, CR, R, LATEX X

User Control Name C, CD, CDR, CR, R, LATEX X

Expiration Date C, CD, CDR, CR, R, LATEX X

Run Date C, CD, CDR, CR, R, LATEX X

Run Time C, CD, CDR, CR, R, LATEX X

Operator C, CD, CDR, CR, R, LATEX X

IQAP ID C, CD, CDR, CR, R, LATEX X

Delete Flag C, CD, CDR, CR, R, LATEX X

Shift C, CD, CDR, CR, R, LATEX X

Cassette / Position C, CD, CDR, CR, R, LATEX X

Reference RBC Count R X

X = Supported by the LH 750 Workstation format.* = Not supported by the LH 750 Workstation format.


L O T # N N • • • N N 16 bytes


PN 4277303D 4-53


Expiration Date Field:

Run Date Field:

Run Time Field:

Operator Field:

IQAP ID Field:

U C N A M E X X X X X • • • X X 12 bytes


E X P D A T E N N / N N / N N 8 bytes Expiration Date. is month/day/year, regardless of the system date configuration.


D A T E N N / N N / N N 8 bytes Run Date. Output is month/day/year, regardless of the system date configuration.


T I M E N N : N N : N N 8 bytes Run Time. Output is on 24 hours, regardless of the system time configuration.

Time output is hours:minutes:seconds


O P E R A T O R X X • • • X 14 bytes


I Q A P X X X • • • X 12 bytes

PN 4277303D4-54


Delete Flag Field:

Shift Field:


Reference RBC Count Field:

Reference WBC Count Field:


D F L A G X 1 byte Run was excluded from statistical calculation on review screen.’Y’ = Run excluded.

SP = Run not excluded.


S H I F T X 1 byte Possible values are 0, 1, 2, or 3. Reference the Online Help for more information.


C A S S P O S A C C C C P P 7 bytes CASS/POS output is cassette number (‘C’) / cassette position (‘P’).Data values for ‘A’ are: ‘A’ - Automatic sampling ‘M’ - Manual sampling


R R B C X X X X X 5 characters numeric data max.


R W B C X X X X X 5 characters numeric data max.

PN 4277303D 4-55


Control CBC Parameters Group FieldsTable 4.44 identifies the field names, test types, and applicable format of Control CBC Parameters Group transmissions.

Each field in the Control CBC Parameters Group will have the following format:

Result Format: **


Numeric Data:**


Table 4.44 Control CBC Parameters -- Group 35


WBC C, CD, CDR, CR X

RBC C, CD, CDR, CR X

HGB C, CD, CDR, CR X

HCT C, CD, CDR, CR X

MCV C, CD, CDR, CR X

MCH C, CD, CDR, CR X

MCHC C, CD, CDR, CR X

RDW C, CD, CDR, CR X

PLT C, CD, CDR, CR X

PCT C, CD, CDR, CR X

MPV C, CD, CDR, CR X

PDW C, CD, CDR, CR X


Tag Data Format Data Length CommentsA A A • • A X X • • • X X A A A A See “Data

Format” column.

See Below.**

Result Comments


- - - - - Total voteout

+ + + + + Overrange




PN 4277303D4-56


Flag Data: **

The flag data characters for the Control CBC Parameter Group fields will be as follows as in Table 4.45:

Control DIFF Count Parameters Group FieldsTable 4.46 identifies the field names, test types, and applicable format of Control DIFF Count Parameters Group transmissions.

Each field in the Control DIFF count Parameters Group will have the following format:

Note: NRBC# will only be present if the NRBC Suspect Message was triggered.

Result Format: **

Table 4.45 Flag Data for Control CBC Parameters Group Fields


R Review parameter.H Exceeds expected range high limitL Exceeds expected range low limit

* MCV threshold failureP Partial aspiration/blood detector disabled


Table 4.46 Control DIFF Count Parameters -- Group 36


LY# CD, CDR XMO# CD, CDR XNE# CD, CDR XEO# CD, CDR XBA# CD, CDR X

NRBC# CD, CDRX = Supported by the LH 750 Workstation format.



See Below.**




PN 4277303D 4-57



Numeric Data:**


Flag Data: **

The flag data characters for the DIFF Count Parameter Group fields will be as follows in Table 4.47:

Control DIFF Percent Parameters Group FieldsTable 4.48 identifies the field names, test types, and applicable format of Control DIFF Percent Parameters Group transmissions.

Result Comments


????? Invalid data: could not calculate DIFF#.


Table 4.47 Flag Data for DIFF Count Parameter Group Fields


R Review parameter.

H Exceeds expected range high limit

L Exceeds expected range low limit



Table 4.48 Control DIFF Percent Parameters -- Group 37


LY% CD, CDR X

MO% CD, CDR X

NE% CD, CDR X

EO% CD, CDR X

BA% CD, CDR X

NRBC% CD, CDR


PN 4277303D4-58


Each field in the Control DIFF Percent Parameters Group will have the following format:

Result Format: **


Numeric Data:**


Flag Data: **

The flag data characters for the DIFF percent Parameter Group fields will be as follows in Table 4.49:

Control RETIC Parameters Group FieldsTable 4.50 identifies the field names, test types, and applicable format of Control RETIC Parameters Group transmissions.



See Below.**

Result Comments


+++++ Sum of DIFF% parameter results not equal to 100% +0.1: : : : : Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)

Table 4.49 Flag Data for Diff Percent Parameter Group Fields




Table 4.50 Control RETIC Parameters -- Group 38


RET% CDR, CR, R X




PN 4277303D 4-59


Each field in the Control RETICS Parameters Group will have the following format:

Result Format: **


Numeric Data:**


Flag Data: **

The flag data characters for the Control RETIC Parameter Group fields will be as follows in Table 4.51:

RET# CDR, CR, R XMRV CDR, CR, R XIRF CDR, CR, R XMI CDR, CR, R *OTHER CDR, CR, R *

X = Supported by the LH 750 Workstation format.* = Not supported by the LH 750 Workstation format.



See Below.**

Result Comments



RET# >999.9 x 106cells/µL

RET% >100%


Table 4.50 Control RETIC Parameters -- Group 38




PN 4277303D4-60


DIFF GraphicsTable 4.52 identifies the field names, test types, and applicable format of DIFF Graphics Group Field transmissions.

If DIFF graphic transmission is disabled, this group will NOT be transmitted.

DIFF Graphics Field:

Retic GraphicsTable 4.53 identifies the field names, test types, and applicable format of Retic Graphics Group Field transmissions.

If Retic graphic transmission is disabled, this group will NOT be transmitted.

Retic Graphics Field:

Table 4.51 Flag Data for Control RETIC Parameter Group Fields




Table 4.52 DIFF DataPlot -- Group 50


DIFF graphics CD, CDR X



D I F F H H H • • • H H H Variable .

Table 4.53 Retic DataPlot -- Group 51


Retic graphics CDR, CR, R X



R E T G H H H • • • H H H Variable

PN 4277303D 4-61

LH SERIES WORKSTATION TO HOST COMMUNICATIONSHOST PARSING OF TRANSMITTED DATA

4.4 HOST PARSING OF TRANSMITTED DATACoulter reserves the right to make additions, deletions, and/or change the order of parameters in future releases. However, if the communications protocol is adhered to as specified in this document, future releases will not require reprogramming on the part of the host.

We do not guarantee the appearance of any particular parameter. Neither do we guarantee any particular order to the transmitted data. This ensures that any program written to parse an existing transmission format will not have to be restructured if it is designed properly.

Note that each information item is an ASCII character string. Because of delimiters, field terminators and so on, these information strings may be extracted from the transmitted data block without having to identify the strings at that time.

PN 4277303D4-62

5
5HOST TO LH SERIES WORKSTATION COMMUNICATIONS(HOST ToDo LIST)
5.1 DATA-LINK

ProtocolThe LH Series Workstation requires full handshaking to receive data. The protocol is similar to that used for LH Series Workstation to Host transmissions with the SYN character replaced with the ENQ character.

The data is transmitted as a sequence of up to 255 blocks of data of 256 [or 128] bytes each. Generally, these blocks contain 256 data bytes each, but due to the unique needs of differing hosts, the system is configurable to allow shorter blocks with 128 data bytes. The final block must be padded with ASCII spaces (20H) if it has less than the number of data bytes necessary to complete.

.

Host Computer (SENDER) LH Series Workstation (RECEIVER)

SYN indicates receiver busy, prepare to receive sample(s) record.

2-byte data block

(NAK indicates receiver abort}

After each block

“All Done”

ENQ

ENQ/SYN

X X

ACK/NAK

Send block and await response. Repeat data block and response until done.

STX 2-byteblk number

Exactly 256 or 128 data bytes

4-byteCRC

ETX

ACK/NAK/ENQ

ENQ

ACK/NAK/ENQ

PN 4277303D 5-1

HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)DATA-LINK

The LH Series Workstation ACKs or NAKs each block sent by the Host. If the LH Series Workstation NAKs a block, the Host must retransmit that block. If the Workstation NAKs the same block 3 times in a row, the Workstation aborts and is then prepared to receive the next transmission. If the LH Series Workstation sends an ENQ after the reception of any block, the Host must retransmit all blocks starting with the first, but should not resend the initial ENQ character. Some common reasons why the LH Series Workstation would NAK a block are: bad block sequence number, incorrect block byte count, CRC mismatch, etc. If the Data Link transmission successfully completes, the LH Series Workstation then transmits a DLE character followed by a single ASCII character indicating whether the transmission is accepted or rejected at the presentation level. The ASCII characters following the DLE are listed below:

The following demonstrates the above protocol:

Host Computer (SENDER) LH Series Workstation (RECEIVER)

ENQ (ready)

send data blocks

DLE

Accept / Reject

ENQ (go ahead) orSYN (busy)

# of data blocks(2 bytes)

ACK (read to receive) or NAK (receiver abort)

DataBlock

ACK (block received ok) or for each blockNAK (retransmit this block) orENQ (retransmit all blocks, starting with block #1)

PN 4277303D5-2

HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)DATA-LINK 5

Return Codes

For the Delete request, codes A, B, C, and D still apply. In addition to these, the following codes are returned for the Delete Function only:

“G” Error in Patient ID field format

“H” Error in Test field format

“I” ToDo list Item not Deleted

•

•

•ENQ (all done)

ACK (transmission accepted) orNAK (transmission rejected) (Presentation Level Response)DLE‘A’ (transmission accepted, ready for next) or‘B’ (transmission accepted, do NOT send more) or‘C’ (transmission rejected, retry) or‘D’ (transmission rejected, abandon) or*‘E’ (transmission rejected, at least one duplicate

pending test request in sample request)

‘F’ (transmission rejected, patient ID already exists in the LH database)

*Please Note: User may select to replace DLE ‘E’ with DLE ‘C’ in the LIS section of the Communications Screen in the LH 750 Workstation System Setup. This option is provided for backward compatibility and is not the optimum configuration for the interface.

PN 4277303D 5-3

HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)PRESENTATION

Data Block Structure

5.2 PRESENTATION

Message Structure

The data which is blocked for transmission will consist of a sequence of ASCII lines each terminated by a CR/LF pair. The block boundaries for the 256 [or 128] byte blocks would have no special significance with respect to this data. The data may be thought of as a large ASCII buffer which is being sent 256 [or 128] bytes at a time.

Byte # # of bytes

1 STX 1 byte

2 BLK NBR MS CHAR 1 byte

3 BLK NBR LS CHAR 1 byte

4 DATA BYTES•

•

•

256 (128) Cbytes R

C

259 (131)

260 (132) CRC MSB MS CHAR 1 byte

261 (133) CRC MSB LS CHAR 1 byte

262 (134) CRC LSB MS CHAR 1 byte

263 (135) CRC LSB LS CHAR 1 byte

264 (136) ETX 1 byte

PREAMBLE INTRO FIELD 1 0 0 0 FIELD N

PreambleField

SOHCR LF2-byte

num fields

“00” to “FF”

PN 4277303D5-4

HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)PRESENTATION 5

The 2-byte num fields indicate the number of fields that follow the preamble field, that is, the intro field plus all data fields.

The record type indicates the target data set, for example, "WL" for ToDo list (TDL).

The operation code indicates the action to be performed:

The ASCII Tag is unique to each field within each record type.

DLE Responses From The Workstation And Effects After A ToDo List DownloadINTRO FIELD: AD Add or Update

1. Conditions prior to ToDo List Download - No samples are in the ToDo list with the same Primary Identifier(s) and no samples are in the ToDo List or Completed list with the same Patient ID.

a. Workstation Response - DLE A or DLE B

b. Effect - Add the downloaded request to the ToDo list.

2. Conditions prior to ToDo List Download - No sample(s) are in the ToDo list with the same Primary Identifier(s) but there are sample(s) are in the ToDo List and/or Completed list with the same Patient ID.


b. Effect - Add the downloaded request to the ToDo list. Update the Last Name, First Name, Gender and Birth Date for all samples in the ToDo List and/or Completed list that have the same Patient ID.

3. Conditions prior to ToDo List Download - Sample(s) are in the ToDo list with the same Primary Identifier(s) and sample(s) are in the ToDo List and/or Completed list with the same Patient ID.

a. Workstation Response - DLE+E, or DLE+C if the workstation was configured to replace DLE+E with DLE+C

IntroField

CR LF2-byterecord type

2-byteoperation

For ToDo list“WL”

“AD”

“UN”

“RU”

“DE”

EachField

ASCIITag

ASCIIData CR LF

Repeat until all fields given.

PN 4277303D 5-5

HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)PRESENTATION

b. Effect - Update the Last Name, First Name, Gender and Birth Date for all samples in the ToDo list and/or Completed List that have the same Patient ID.

4. Conditions prior to ToDo List Download - The downloaded ToDo list format is not correct.

a. Workstation Response - DLE+C or DLE+D.

b. Effect - No modifications are made to the ToDo list or to the Completed list.

INTRO FIELD: UN Unique

1. Conditions prior to ToDo List Download - No samples are in the ToDo list with the same Primary Identifier(s) and no samples are in the ToDo List or Completed list with the same Patient ID. If samples are deleted or edited to remove the patient ID then the Workstation will have to be rebooted before downloading a sample with the same patient ID using the Unique intro tag. Logging off and back on is not sufficient to allow the reuse of a patient ID.



2. Conditions prior to ToDo List Download - No sample(s) are in the ToDo list with the same Primary Identifier(s) but there are sample(s) are in the ToDo List and/or Completed list with the same Patient ID.

a. Workstation Response - DLE F



a. Workstation Response - DLE F


4. Conditions prior to ToDo List Download - The downloaded ToDo list format is not correct.



INTRO FIELD: RU Reuse

1. Conditions prior to ToDo List Download - No samples are in the ToDo list with the same Primary Identifier(s) and no samples are in the ToDo List or Completed list with the same Patient ID.



2. Conditions prior to ToDo List Download - No sample(s) are in the ToDo list with the same Primary Identifier(s) but there are sample(s) in the ToDo List and/or Completed list with the same Patient ID.

PN 4277303D5-6

HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)MESSAGE DEFINITION 5


b. Effect - Add the downloaded request to the ToDo list. Remove the Last Name, First Name, Gender and Birth Date for all samples in the ToDo List and/or Completed list


a. Workstation Response - DLE+E, or DLE+C if the workstation was configured to replace DLE+E with DLE+C

b. Effect - Remove the Last Name, First Name, Gender and Birth Date for all samples in the ToDo list and the Completed list that have the same Patient ID.

4. Conditions prior to ToDo List Download -. The downloaded ToDo list format is not correct.


b. Effect - No modifications are made to the ToDo list or to the Completed list

INTRO FIELD: DE Delete

DE in this field is a command by the host to delete one or more test entries in the LH Series Workstation ToDo List. See Section 5.4, Delete Message, for a specification for this function.

5.3 MESSAGE DEFINITIONTable 5.1 lists the fields and the format to which they apply. The table also identifies fields that require data transmission in another field in order to be included in the test request. If data is not in the associated field, the LH Series Workstation ignores the field. For example, if a Patient ID is not transmitted, the LH Series Workstation ignores any data included in the Patient Last Name field.

After the Table is the list of possible fields, their field width and the appropriate ASCII Tag to be used for transmission.

A field will have a variable length TAG preceding the data separated by one or more SP (20H) or HT (09H) characters. A field tag must be transmitted with data. If a field tag is transmitted without data, the Workstation rejects the whole sample request. If an invalid or unrecognized tag is transmitted, the LH Series Workstation will ignore the field and go to the next field provided the field to be ignored has the correct field format (i.e. is terminated by a CRLF).

Table 5.1 Message Definition Table

Field NameLH 750 Workstation

Field Requires

Patient ID X Test

Patient Last Name X Patient ID

Patient First Name X Patient ID

Sequence Number X Test

Birth Date X Patient ID

PN 4277303D 5-7

HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)MESSAGE DEFINITION

Only one sample’s demographics can be sent per transmission.

FIELDS CANNOT HAVE LEADING OR TRAILING SPACES (MESSAGE WILL BE REJECTED).

Patient ID Field:

Sex X Patient ID

Location X Test

Physician Last Name X Test

Physician First Name X Test

User Field 1 X Test

User Field 2 X Test

User Field 3 X Test

Comments X Test

Report Profile X Test

Sample Drawn Date X Test

Sample Drawn Time X Test

Test X

Slide Request X Test



I D X X X • • • X X 16 bytes Patient ID is 16 characters, alphanumeric.Value in Intro field determines what is done:

AD

UN

RU

See Heading 5.2, PRESENTATION for details.

Table 5.1 Message Definition Table

PN 4277303D5-8


Patient Last Name Field:

Patient First Name Field:

Sequence Number Field:

Birth Date Field:

Sex Field:


P L X X X • • • X X 16 bytes Patient Last Name is 16 characters, alphanumeric.Requires Patient ID.


P F X X X • • • X X 16 bytes Patient First Name is 16 characters, alphanumeric.Requires Patient ID.


S N N N N N N N 6 bytes Sequence Number


B D N N / N N / N N N N 10 bytes Date format must be the same as the Workstation configuration. Any of the following formats can be used. DD/MM/YYYY, MM/DD/YYYY, or YYYY/MM/DD. Requires Patient ID.


S X A 1 byte Data Value:

‘M’ - Male

‘F’ - Female

‘U’ - Unknown

‘O’ - Other (equivalent to ‘Unknown’)Requires Patient ID.

PN 4277303D 5-9


Location Field:

Physician Last Name Field:

Physician First Name Field:

User Field 1 Field:

User Field 2 Field:

User Field 3 Field:

Comments Field:


L N X X X • • • X X 16 bytes


D L X X X • • • X X 16 bytes Physician Last Name is 16 characters, alphanumeric.


D F X X X • • • X X 16 bytes Physician First Name is 16 characters, alphanumeric.


U 1 X X X • • • X X 16 bytes Requires Test.






C M X X X • • • X X 64 bytes Requires Test.

PN 4277303D5-10


Report Profile Field:

Sample Drawn Date Field:

Sample Drawn Time Field:


R P N N 2 bytes Report Profile possible values are from “01" to "10".


E D N N / N N / N N 8 bytes Date format must be the same as the Workstation configuration. Any of the following formats can be used. DD/MM/YY, MM/DD/YY, or YY/MM/DD. Requires Test.

If the combined Date/Time of the sample is annotated later than when the actual Date/Time the sample is received, the sample drawn will be rejected.


E T N N : N N 5 bytes Time output is on 24 hours, regardless of the system time configuration. Time output is hours:minutes.Requires Test.

If the combined Date/Time of the sample is annotated later than when the actual Date/Time the sample is received, the sample drawn will be rejected.

PN 4277303D 5-11


Profile Selection:

Data transmission reflects profile selection. Parameters not in the selected profile will not be transmitted. Test blocks (e.g., CBC, DIFF, etc.) which are not in the current profile will not be transmitted. If a complete test block is omitted from the current profile, the General Information Block (for sample identification) will still be sent.

Test Field:

Slide Request Field:


T S X X X • • • X X Up to 64 bytes This field indicates test requests. Format for a test request is: ”TEST,TUBE ID,CASS/POS” or ”TEST,TUBE ID,” or ”TEST,CASS/POS” . The commas are required as separators. Note that a comma is required after TUBE ID, even if it is not followed by a CASS/POS.See Table 5.2 for possible values.Required for transmission.

The TS tag can repeat itself for as many tests as may be required. Only one test request can be sent per test tag occurrence. See Table 5.3 for sample and text request combinations.


S R N 1 byte Valid entries are 0 and 1. For a Retic Mode request, the only valid entry for the Slide Request field is 0. If a value > 1 is sent, this is converted by the LH 750 Workstation to a value of 1.

PN 4277303D5-12


Table 5.2 Possible Values and Examples of Test Requests

Test Tag LH 750 Workstation Comment Example

CBC X CBC only test. “TS CBC,000101”

“TS CBC,123456789012,”

“TS CBC,123456789012,000101”

DIFF X CBC/DIFF test “TS DIFF,000101”

“TS DIFF,123456789012,”

“TS DIFF,123456789012,000101”

CDR X CBC/DIFF/Retic test “TS CDR,000101”

“TS CDR,123456789012,”

“TS CDR,123456789012,000101”

RETIC X Retic only test “TS RETIC,000101”

“TS RETIC,123456789012,”

“TS RETIC,123456789012,000101”

CR X CBC/Retic test “TS CR,000101”

“TS CR,123456789012,”

“TS CR,123456789012,000101”

X = Supported by the LH 750 Workstation format. *CASS POS is six characters TUBEID is no more than 16 characters

Table 5.3 Possible Combinations of Sample Requests with Multiple Test Requests

Test CBC DIFF CDR RETIC CR

CBC NO NO NO YES NO

DIFF NO NO NO YES NO

CDR NO NO NO NO NO

RETIC * YES YES NO NO NO

CR NO NO NO NO NO

* For a Retic Mode request, the only valid entry for the Slide Request field is 0.

PN 4277303D 5-13

HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)DELETE MESSAGE

A sample request with multiple test requests for CBC+DIFF or Retic only, would appear similar to the following:

.

.

.“TS DIFF,123456,000101”“TS RETIC,123,000203”...

5.4 DELETE MESSAGEWhen the Intro Field of a ToDo List upload from the host contains the Operation string “DE” (see page 5-77), the host has requested the deletion of one or more entries from the LH Series Workstation ToDo List. The LH Series Workstation allows deletion of preassigned test orders from the ToDo List by the host according to;

Patient ID

Sample ID Number

Cassette Position

Test Type

The Sample ID, Cassette Position and Test Type are part of the Test Field. The formats for the Patient ID and Test fields are described in 5.3 Message Definitions. The Patient ID and Test field are the only fields of the message definition section used in the Deleted Function. All others are ignored and need not be sent.

In the Deleted context, an ID or TS Tag followed by a CRLF will be interpreted as an empty Patient ID or Test Field.

If the Patient ID field and the Test field are empty, the Workstation deletes all entries from the ToDo List.

According to the above definition, these fields are empty when they have the following form:

It is possible for a pre assignment to be made to the ToDo List that has no Patient ID. If the host requests deletion of this pre assignment, or some part of it, the transmission of the deletion request must contain no Patient ID field. Note that this is not the same as an empty Patient ID field. No field with an ID tag should be sent. See example 10.

On page 5-83, the Data Format of the Test field is given as “TEST,TUBE ID, CASS/POS” or “TEST,TUBE ID,” or “TEST,CASS/POS”. The TUBE ID here is the SAMPLE ID. The commas

Patient ID Field: I D CR LF See Pages 5-5 & 5-9

Test Field: T S CR LF See Pages 5-5 & 5-13

PN 4277303D5-14

HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)DELETE MESSAGE 5

are required as separators. Note that a comma is required after TUBE ID even if it is not followed by a CASS/POS. This Test Field Data Format has additional flexibility in the Delete Function only.

Note: “TEST,TUBE ID,” represents an empty CASS/POS indicator and “TEST,CASS/POS” represents and empty TUBE ID indicator.

Additional format flexibility in the Delete Function only is as follows.

“,TUBE,ID,CASS/POS”shall represent an empty TEST indicator.

“,,CASS/POS” shall represent empty TEST and TUBE ID indictors.

“,TUBE ID,” shall represent empty TEST and CASS/POS indicators.

“TEST,,” shall represent empty TUBE ID and CASS/POS indicators.

“,,” shall represent empty TEST,TUBE ID, and CASS/POS indicators.

Note: The last entry has the same effect as an empty Test field.

In the Delete context, an Empty Indicator is interpreted to refer to all values of that indicator relative to the next highest indicator in the ToDo List. For example; If the TUBE ID indicator is not empty and the TEST indicator is empty, this is interpreted to mean “delete all TEST’s preassigned to this TUBE ID”. An empty Test field refers to all preassigned tests relative to a given Patient ID (this is also true for the Test Field Data Format”,,”).

It should be noted again, that if there is no Patient ID field, this is not the same as an empty Patient ID field. This is interpreted as referring to a preassignment with no Patient ID.

The following table illustrates the variables.

PN 4277303D 5-15

HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)DELETE MESSAGE

* This field was not transmitted.

Note: TS_<CR><LF> is to TS_, , <CR><LF>

Table 5.4 Patient ID Deletions

Example Patient ID Test ID Action

1 ID_<CR><LF> TS_<CR>< LF> Delete all entries of all types in ToDo list.

2 ID_123456<CR><LF>

TS_<CR><LF> Delete all Patient ID “123456” entries of all test types in ToDo list.

3 ID_<CR><LF> TS_CBC,,<CR><LF> Delete all CBC tests from all Patient ID entries in ToDo list.

4 ID_123456<CR><LF>

TS_CBC,,<CR><LF> Delete all CBC tests from Patient ID 123456.

5 ID_123456<CR><LF>

TS_CDR,987654,<CR>< LF> Delete CDR test from Tube ID “987654”and all Cass/Pos for Patient ID 123456.

6 ID_123456<CR><LF>

TS_CDR,,001001,<CR>< LF> Delete all Patient ID “123456” entries of CDR test type in ToDo list with Cass/Pos “001001”.

7 ID_123456<CR><LF>

TS_CBC,987654,001001, <CR>< LF>

Delete all Patient ID “123456” entries of CBC test type in ToDo list with Tube ID “987654” and Cass/Pos “001001”.

8 ID_<CR><LF> TS_CBC,001001 Delete CBC test for all the Tube IDs with Cass/Pos “001001”for all Patient IDs.

9 ID_<CR><LF> * Delete all entries of all types in ToDo list.

10 * TS_ , ,<CR><LF> Delete all entries in ToDo list without Patient IDs.

11 ID_<CR><LF> TS_ ,ID12345,<CR><LF> Delete Tube ID from all test types.

PN 4277303D5-16

6
6ASCII Tables
6.1 7-BIT ASCII CODES

6.2 VALID HOST COMMUNICATIONS ASCII CODES

Bold used only for Data-link control.

+ ) ) ) ) ) ) 0 0 0 0 1 1 1 1* Ú

+) ) ) ) ) 0 0 1 1 0 0 1 1

* +Ú

) ) ) ) 0 1 0 1 0 1 0 1

! ! !

7 6 5 4 3 2 1

X X X 0 0 0 0 NUL DLE SP 0 @ P ` pX X X 0 0 0 1 SOH DC1 ! 1 A Q a qX X X 0 0 1 0 STX DC2 “ 2 B R b rX X X 0 0 1 1 ETX DC3 # 3 C S c sX X X 0 1 0 0 EOT DC4 $ 4 D T d tX X X 0 1 0 1 ENQ NAK % 5 E U e uX X X 0 1 1 0 ACK SYN & 6 F V f vX X X 0 1 1 1 BEL ETB ‘ 7 G W g wX X X 1 0 0 0 BS CAN ( 8 H X h xX X X 1 0 0 1 HT EM ) 9 I Y I yX X X 1 0 1 0 LF SUB * : J Z j zX X X 1 0 1 1 VT ESC + ; K [ k {X X X 1 1 0 0 FF FS , < L \ l |X X X 1 1 0 1 CR GS - = M ] m }X X X 1 1 1 0 SO RS . > N ^ n `X X X 1 1 1 1 SI US / ? O _ o DEL

+ ) ) ) ) ) ) 0 0 0 0 1 1 1 1* Ú

+) ) ) ) ) 0 0 1 1 0 0 1 1

* +Ú

) ) ) ) 0 1 0 1 0 1 0 1

! ! !7 6 5 4 3 2 1X X X 0 0 0 0 DLE SP 0 @ P ` pX X X 0 0 0 1 SOH DC1 ! 1 A Q a qX X X 0 0 1 0 STX “ 2 B R b rX X X 0 0 1 1 ETX # 3 C S c sX X X 0 1 0 0 $ 4 D T d tX X X 0 1 0 1 ENQ NAK % 5 E U e uX X X 0 1 1 0 ACK SYN & 6 F V f vX X X 0 1 1 1 ‘ 7 G W g wX X X 1 0 0 0 ( 8 H X h xX X X 1 0 0 1 HT ) 9 I Y I yX X X 1 0 1 0 LF * : J Z j zX X X 1 0 1 1 + ; K [ k {X X X 1 1 0 0 , < L \ l |X X X 1 1 0 1 CR - = M ] m }X X X 1 1 1 0 . > N ^ n `X X X 1 1 1 1 / ? O _ o DEL

PN 4277303D 6-1

ASCII TablesVALID HOST COMMUNICATIONS ASCII CODES

PN 4277303D6-2

7
7CRC
7.1 CRC ALGORITHMThe CRC algorithm used is a modified CCITT CRC16 algorithm and is the same as used in the STKS™ analyzer.

The polynomial for this algorithm is: X**16 + X**12 + X**5 + 1.

Note: D = current data byte that is input to the algorithm.

CRCLSB,CRCMSB = data byte. Least significant and most significant CRC accumulator bytes.

x>>n means x is shifted n bits to right or is the same as x divided by 2n.

x<<n means x is shifted n bits to left or is the same as x multiplied by 2n.

//------------------------------------------------------------------------------------------------------------------

at beginning,CRCLSB = 0FFH (octal 377) (decimal 255) CRCMSB = 0FFH (octal 377) (decimal 255)

then for each data byte in a block,X = D XOR CRCMSBX = X XOR ( X >> 4 )CRCMSB = CRCLSB XOR ( X >> 3 ) XOR ( X << 4 )CRCLSB = X XOR ( X << 5 )

and at end,CRCLSB = CRCLSB XOR 0FFHCRCMSB = CRCMSB XOR 0FFH

//------------------------------------------------------------------------------------------------------------------

PN 4277303D 7-1

CRCCRC EXAMPLE WRITTEN IN ASM86

7.2 CRC EXAMPLE WRITTEN IN ASM86The following is an example of CRC16 code written in assembler for 8086:

;-------------------------------------------------------------------------------------------------------------------

GET_CRC_BYTES PROCPUSH CX

;;This is the Algorithm used (CCITT CRC16):;; X = D XOR CRCMSB; X = X XOR ( X >> 4 ); CRCMSB = CRCLSB XOR ( X >> 3 ) XOR ( X << 4 ); CRCLSB = X XOR ( X << 5 );;Data arrives in AL.;Finished CRC is in BX ( BH = MSB, BL = LSB );To use this algorithm:; 1. Initialize BX to 0FFFFH.; 2. At end:; CRCLSB = CRCLSB XOR 0FFH.; CRCMSB = CRCMSB XOR OFFH.;

XOR AL,BH ;X=D XOR CRCMSB (AL=X) <--- 1

MOV AH,AL ;Save X for later in AHSHR AL,4 ;Then AL=X >> 4

XOR AL,AH ;X=X XOR ( X >> 4 )<--- 2

MOV AH,AL ;Save XMOV CH,AL ;Save XSHR AL,3 ;Then AL= ( X >> 3 )XOR AL,BL ;AL=CRCLSB XOR ( X >> 3 )SHL AH,4 ;AH=( X << 4 )

XOR AL,AH ;CRCMSB=CRCLSB XORMOV BH,AL ; ( X >> 3 ) XOR ( X << 4 )<--- 3

MOV AL,CH ;Recover X in ALSHL AL,5 ;AL=( X << 5 )XOR AL,CH ;AL=X XOR ( X << 5 )

MOV BL,AL ;CRCLSB=X XOR ( X << 5 )<--- 4

POP CXRET

GET_CRC_BYTES ENDP;-------------------------------------------------------------------------------------------------------------------

PN 4277303D7-2

CRCCRC EXAMPLE WRITTEN IN C 7

7.3 CRC EXAMPLE WRITTEN IN CThe following is an example of CRC16 code written in C.//------------------------------------------------------------------------------------------------------------------void calc_crc ( unsigned char DataByte ){DataByte ^= crcmsb;

DataByte ^= ( DataByte / 16 ); crcmsb = crclsb ^ ( DataByte / 8 ) ^ ( DataByte * 16 ); crclsb = DataByte ^ ( DataByte * 32 );

}//------------------------------------------------------------------------------------------------------------------

PN 4277303D 7-3

CRCCRC EXAMPLE WRITTEN IN C

PN 4277303D7-4

8
8GRAPHIC RECONSTRUCTION
8.1 RBC, WBC THRESHOLD, AND PLT HISTOGRAMSThe histograms display a simple line graph which is representative of the count of events occurring in each A/D channel (or voltage level). The histograms are transmitted as 512 ASCII bytes for RBC (256 bytes for PLT, 112 bytes for WBC and 512 bytes for WBC Full Histogram) representing a 256-byte for RBC (128-byte for PLT, 56 bytes for WBC and 256 bytes for WBC Full Histogram) Hex array. It takes two ASCII bytes to represent one Hex byte.

Each Hex byte represents a scaled count for each channel 0-255 (14-142 for PLT). The histograms can be constructed by plotting the count for each channel then drawing a line between plots.

To display (or print) the histogram you must plot the channel vs. count. The channel represents the X-axis while the count is the Y-axis (see Figure 8.1). There are 128 channels for the platelet histogram, 256 channels for RBC, 56 channels for WBC and 256 channels for WBC Full Histogram. The value range is 14-255 (0D-FF hex) for the platelet histogram and 0-255 (00-FF hex) for the WBC and RBC histograms.

Figure 8.1 Plotting RBC, WBC Threshold and PLT Histograms

8.2 LH 750 DATAPLOT FORMATThe 2-dimensional dataplots communicate a greater amount of information to the viewer about the events. The following sections describe the format of the dataplot data and provide a how-to example of displaying the dataplot data on a raster device such as a video display or printer.

DataplotsThe dataplots consist of two major components: dataplot data and rendering information. Taken together, these two components allow a receiving application to display the dataplots in the same fashion as the LH Series Workstation.

The dataplot portion of the host transmission consists of a dataplot data header and one dataplot data block.

CHANNEL

COUNT

<< x

y

PN 4277303D 8-1

GRAPHIC RECONSTRUCTIONLH 750 DATAPLOT FORMAT

Dataplot Data HeaderThe dataplot data header tells the receiving application how large the dataplot transmission is and how many dataplot data blocks are contained in the transmission. A complete dataplot transmission, including the dataplot data header, is illustrated in Figure 8.2.

Figure 8.2 Dataplot Transmission

Dataplot Data Header ElementsThe dataplot data header contains two elements: the total length of the dataplot transmission and the number of dataplot data blocks in the transmission. The data are represented as one 16-bit integer which indicates the total size of the dataplot transmission and one 8-bit integer which indicates the total number of dataplot data blocks in the transmission (between 1 and 4). Only one block is supported currently, but up to four may be used in future software. See Figure 8.3 for details.

Figure 8.3 Dataplot Data Header

Dataplot Data BlockThe dataplot data block contains both the rendering information and the actual 2-dimensional dataplot data. Each dataplot data block is composed of four subsections: dataplot data block header, rendering information block, dataplot header and the dataplot data. Figure 8.4 illustrates the dataplot data block format.

Figure 8.4 Dataplot Data Block Format

Dataplot data header

Dataplot data block

Transmission Length LSB

Transmission Length MSB

Total Number of Dataplot data blocks

Dataplot data block header

Rendering information block

Dataplot header

Dataplot data

PN 4277303D8-2

GRAPHIC RECONSTRUCTIONLH 750 DATAPLOT FORMAT 8

Dataplot Data Block HeaderThe dataplot data block header tells the receiving application about the type of dataplot being transmitted as well as details about any included rendering information. See Figure 8.5. The dataplot data block header has the following format:

Figure 8.5 Dataplot Data Block Header

Rendering Information BlockThe rendering information block contains information the receiver can use to reconstruct the dataplot as it was displayed by the LH Series Workstation. The dataplots are color-coded to identify the population and dithered to code the density. The rendering information block contains color palettes and dither pattern bitmaps used by the LH Series Workstation software when displaying or printing the dataplots. It is highly recommended that host software employ the same scheme when displaying the dataplots to maintain consistency of operator conclusions across platforms. See Figure 8.6. The structure of the rendering information block is as follows:

Dataplot Type code Dataplot Type Code is an 8-bit integer which represents the run type of the sample which produced this dataplot. It will be one of the following values:

SC_TYPE_5DIFF (0x01) is 5 Part Differential Dataplot.

SC_TYPE_RETIC (0x02) is Reticulocyte Dataplot.

SC_TYPE_CD4 (0x04) is CD4‡ Subset Dataplot.

SC_TYPE_CD8 (0x05) is CD8‡ Subset Dataplot.

Dataplot Option code Dataplot Option Code is an 8-bit integer which contains option flags that pertain to the current dataplot. The following flags are currently defined and can be combined into Dataplot Option Code with a logical or when applicable:

SO_COMPRESSED (0x01) indicates that the dataplot has been compressed. This option is not supported currently, but may be available in future software.‡

SO_HAS_RENDER_INFO (0x02) indicates that a valid rendering information block follows.

Dataplot width (LSB) Dataplot width is a 16-bit integer which specifies the width of the dataplot.

Dataplot width (MSB)

Dataplot height (LSB) Dataplot height is a 16-bit integer which specifies the height of the dataplot.

Dataplot height (MSB)‡This symbol indicates that at the time of this release this feature was not available.

PN 4277303D 8-3


Figure 8.6 Rendering Information Block

Rendering Information Block HeaderThe rendering information block header contains fields which indicate the size and format of the bitmaps and the color palette. It is sent on the first transmission after a user log-on and a shutdown/restart occurs. As notification is not provided when a shutdown/restart is initiated, the rendering block transmission cannot be predicted in advance. Figure 8.7 is an illustration of the rendering information block header.

Figure 8.7 Rendering Information Block Header Format

Color PaletteEach color palette entry contains three 8-bit integers which represent the red, green and blue values for that index as shown in Figure 8.8.

Figure 8.8 Palette Table Entry

Dithered Display Bitmap LibraryThe dithered display bitmap library contains the dithering bitmaps used by the LH Series Workstation to display the dataplots. They indicate the density of a specific cell population. Receiving applications should use these bitmaps when generating displays to ensure that host

Rendering information block header

Color palette

Dithered display bitmap library

Rendering information block options

Rendering Information Block Options may be one or more of the following flags:

RH_OPTIONS_NONE (0x00) indicates that no options are set.

It is an 8-bit integer.

Number of color palette table entries

Number of Color Palette Table Entries is the number of entries in the color palette. It is an 8-bit integer.

Number of bitmap library entries

Number of Bitmap Library Entries is the number of bitmap patterns contained in the Dithered display bitmap library. It is an 8-bit integer.

Bitmap width in pixels Bitmap Width in Pixels is the width of each bitmap in pixels. It is an 8-bit integer.

Bitmap height in pixels Bitmap Height in Pixels is the height of each bitmap in pixels. It is an 8-bit integer.

Red Component

Green Component

Blue Component

PN 4277303D8-4

GRAPHIC RECONSTRUCTIONLH 750 DATAPLOT FORMAT 8

displays will match LH Series Workstation displays even when Coulter updates the dither patterns or population colors. The bitmap format is a standard raster scanned array of pixels. Each value is an 8-bit integer which corresponds to an entry in the color palette described above. Figure 8.9 describes the bitmap format.

Figure 8.9 Bitmap Format

Each bitmap is stored as a column-major array of pixels represented as 8-bit integers. The array contains:

Bitmap width in pixels x Bitmap height in pixels 8-bit integers

Dataplot HeaderThe dataplot header contains instructions of how the dataplot data is to be interpreted. The structure of the dataplot header is shown in Figure 8.10.

Figure 8.10 Dataplot Header

Dataplot data options Dataplot data options may be one of the following flags:

SO_OPTIONS_NONE (0x00) no option have been selected.

It is an 8-bit integer.

Dataplot datum size Dataplot datum size is the size in bits of each point in the dataplot. It is usually 8 which represents a byte. It is an 8-bit integer.

Reserved Reserved for future use and should be ignored. It is an 8-bit integer.

Reserved Reserved for future use and should be ignored. It is an 8-bit integer.

0Bitmap widthin pixels

Bitmap heightin pixels 7306002B

PN 4277303D 8-5


The format of the dataplot is shown in Figure 8.11.

Figure 8.11 Dataplot Data Format

0 Scatterplotwidth

Scatterplotheight 7306003B

PN 4277303D8-6

PN 4277303D

TRADEMARKS

5C Series Control, Beckman Coulter, Coulter, Coulter Clenz, Coulter Counter, Lin-C, Lyse S, S-Cal, Z Series, Zap-Oglobin are trademarks of Beckman Coulter, Inc.

s Getting StartedPN 4277302 (LH 750)PN 624601 (LH 500)

Overview of system hardware and software.

s Host TransmissionPN 4277303

Specifications for transmitting to a host computer

s ReferencePN 4277248 (LH 750)PN 624604 (LH 500)

Use and Function • Installation • Operation Principles • Specifications/Characteristics • Precautions/Hazards • References • Glossary • IndexAvailable in hard copy by request.

s Operator’s Guide/IFUPN 4277249 (LH 750)PN 624602 (LH 500)

Controls and Indicators • Startup • QC • Sample Analysis • Data Analysis • Shutdown • Analyzer CRT Functions • Workstation • Run Samples Display • To Do List • Database • Controls • Setup • AppendicesAvailable in hard copy by request.

s Special Procedures and TroubleshootingPN 4277250 (LH 750)PN 624603 (LH 500)

Calibration • Cleaning Procedures • Replace/Adjust Procedures • Troubleshooting • Error MessagesAvailable in hard copy by request.

s SlideMaker Operator’s GuidePN 4277299 (LH 750)

Use And Function • Installation • Operation Principles • Specifications/characteristics • Operating Procedures • Cleaning/Replacement Procedures • ReferencesAvailable in hard copy by request.

s SlideStainer Operator’s GuidePN 4277300 (LH 750)

Use And Function • Installation • Operation Principles • Specifications/characteristics • Operating Procedures • Cleaning/Replacement Procedures • ReferencesAvailable in hard copy by request.

s Master IndexPN 4277301 (LH 750)PN 624605 (LH 500)

Combined index for Reference, Operator’s Guide, and Special Procedures and Troubleshooting manuals. Comes with hard copy of Operator’s Guide and Special Procedures and Troubleshooting.

r Operator Manuals CD-ROMPN 6418430 (LH 750)PN 624495 (LH 500)

HTML file of the System Help • PDF files of the Reference manual, Instructions for Use, and Special Procedures manual.

The Getting Started booklet and the Host Transmission manual come with your LH Series System.

The information in the Reference manual, Operator’s Guide, Special Procedures and Troubleshooting manual, SlideMaker Operator’s Guide and the SlideStainer Operator’s Guide comes from the Online Help System.

COULTER LH SERIES SYSTEM HARD-COPY DOCUMENTATION

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